Objective To observe the effect ofXuebijing injection on vascular endothelial function and inflammatory factor in the patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods A total of 100 patients with AECOPD were randomly divided into the study and control groups, 50 patients in each group. The control group were given the conventional therapy. The study group were given the Xuebijing injection on the basis of the control group. Both The changes of lung function, vascular endothelial function and inflammatory factor were observed.Results The expression of FVC (3.23 ± 0.83 Lvs. 2.79 ± 0.71 L,t=1.786), PEF (5.36 ± 0.51 L/svs. 4.79 ± 0.46 L/s,t=1.904), FEV1/FVC (59.21 ± 4.38vs. 54.94 ± 4.29,t=1.882), NO (3.84 ± 0.81 ng/mlvs.2.97 ± 0.62 ng/ml,t=1.869) increased significantly in the study group than those in the control group after treatenmt (P<0.05). The VEGF (0.14 ± 0.04 ng/ml vs.0.17 ± 0.05 ng/ml, t=2.097), TNF-a (0.11 ± 0.03 ng/ml vs.0.14 ± 0.02 ng/ml,t=1.903), IL-13 (107.27 ± 44.37 ng/mlvs. 136.54 ± 48.32 ng/ml,t=2.005) in the study group decreased significantly than those in the control group (P<0.05). ConclusionsXuebijing injection could significantly decrease inflammatory factor,and obviously improve vascular endothelial function and lung function,the clinical curative effect is distinct.

Objective To explore variance and resource of intracellular free Ca2+ and extracellular arachidonic acid (AA) in different types of passage cells during the invasion of T. gondii. Methods The variance and resource of extracellular AA and intraceUular free Caz+ of Vero and J774A. 1 cells during the invasion of T. gondii were detected by multi-purpose scintillation counter and laser scanning confocal microscope. Data were analyzed using Chi-square test and t test. Results The intracellular free Ca2+ levels in J774A. 1 and Vero cells were both increased after T. gondii infection. The maximal changes of fluorescence intensity were (1 219.7±58.4)% (P＜0.01) and (356.3±23.6)% (P＜0.05), respectively.The increase of intracellular Ca2+ level in Vero cell was mostly from the release of intracellular Ca2+ store.And the Ca2+ increase in J774A. 1 cell was from both the release of intracellular Ca2+ store and extracellular Ca2+ influx. Extracellular AA levels were significantly increased in both Vero and J774A. 1 cells after T.gondii infection 5.02 and 8. 44 times respectively (t= 3. 124, t = 3. 852, P＜0.01). The AA elevations could be significantly inhibited by phospholipase A2 (PLA2) inhibitor pretreating T. gondii. Conclusions The phospholipase C of phagocytic host cell and PLA2 of T. gondii are activated by T. gondii infection,which results in the increase of intracellular free Ca2+ and extraeellular AA level. Combined actions of Ca2+and AA play a major role in the invasion of T. gondii to host cell. While only PLA2 of T. gondii may be activated in nonphagoeytic host cell.

Objective To study a multiple frequency tympanometry on ear system. Methods Based on the original low frequency and single ingredient SY-IA tympanometry, the study mainly focused on such aspects as multiple frequency probe tone circuit, constant pressure sound control system, micropressor control system and multiple frequency acoustic immitance & phase angle detection circuit. Results Multiple frequency tympanometry on ear system could detect multiple frequency acoustic immitance and phase angle for normal ear, tympanum loosed ear and ossicular chain break ear under different probe tone. Conclusion Experiments show that multiple frequency tympanometry on ear system based on ARM is helpful to the diagnosis of pathological changes which are insensitive to single low frequency tympanometry.

This article discussed the necessity and method of setting up a comparative imaging medicine course, which is considered to be valuable for the future development and creativity of graduate students majored in nuclear medicine and imaging medicine.

Objective To selectively resect the lymph nodes in the endometrial cancer by risk factors evaluation pre-and intra-operation.Methods Three hundred and seventy-four cases who in the Obstetric and Gynecology Hospital Affiliated to Fudan University from Oct.2010 and Dec.2012 were clinically diagnosed as stage Ⅰ pre-and intra-operation,grouped as one (staged Ⅰ A,294 cases) and two (staged Ⅰ B,80 cases),and the situation of lymph nodes metastasis and the related risk factors were analyzed.Results (1) Lymph nodes metastasis were positive in 6 cases in group one (2.0％) and 12 cases in group two(15.0％),and the difference was significant between two groups (x2 =23.054,P =0.000).(2) University analyses showed that pathologic type,grade,myometrial invasion,lymphovascular invasion and cervical gland invastion were the risk factors contributed to the lymphatic metastasis in early staged endometrial cancer (P＜ 0.05).Multivariate analysis showed that myometrial invasion was independent risk factor affecting early metastasis of lymph node in endometrial carcinoma (regression coefficient =2.339,standard error =0.585,OR =10.897,95％ CI 3.273 ～ 32.367,P =0.000).(3)Based on the gynecology oncology group test and risk factors that recent used in different countries and clinical centers,superficial myometrial layer invasion,G2,age＞ 60 years,tumor ≥ 2 cm in diameter as the medium risk factors,and deep myometrial layer invasion,G3,lower portion of the uterus or cervical gland invasion as high risk factors,and patients with two or more medium risk factor (106 cases) and with one or more high risk factors(138 cases) were inclined to develop lymph node metastasis((5.66％ (6/ 106),0(0/130),P =0.04;8.70％ (12/138),2.54％ (6/236),P =0.007).(4) Two hundred and thirteen patients were found with superficial layer invasion by frozen section,and 11 of them were upgraded to Ⅰ B by postoperative pathologic examination.Interestingly,all of the 11 cases were accompanied by two medium or one of high risk factor.Conclusion Low incidence of lymph nodes involvement is found in stage Ⅰ endometrial cancer,especial stage Ⅰ A.Myometrial invasion is closely linked to lymph node metastasis,thus,lymphadenectomy should routinely be carried out in endmetrial cancer diagnosed as Ⅰ B whether pre-or intraoperation.As in stage Ⅰ A,unless two medium and one high risk factors are found.

Objective To keep the intensity of sound constant and reduce the effect of ear canal difference on detection process.Methods Real-time detection of the probe tone in ear canal was carried out and by control ling the output circuit of the probe tone through the digital signal compensation algorithm,to realize real-time digital tympanometry of ear system and the function of constant pressure control.Results The results showed that the probe tone remained constant at 226 Hz for the measurement of middle ear and tympanometry was accurate.Conclusion The study simplified system configuration and provides basis for the clinical application.

ObjectiveTo explore the relationship among resilience,attribution,and coping style for freshmen.Methods 977 freshmen were surveyed with Connor-Davidson Resilience Scale,Multidimensional-Multiattributional Causality Scale,and Simplified Coping Style Questionnaire,then compared different freshmen's resilience,attribution,and coping style,and analyzed their relationship.ResultsThe city freshmen showed significant higher resilience(68.88 ± 10.57 ),academic external attribution (21.14 ± 6.01 ),and interpersonal external attribution ( 22.87 ± 6.42 ) than rural freshmen ( ( 67.51 ± 10.43 ),( 19.81 ± 5.88 ),( 21.86 ± 5.88 ) respectively,t =2.03,3.48,2.57).Vocational freshmen showed significant higher academic internal attribution ( 28.68 ± 5.30 )and interpersonal internal attribution (26.81 ± 5.82) than undergraduate freshmen ( ( 27.61 ± 5.47 ),( 25.53 ±5.92),t =3.00,3.30).The only children showed significant higher resilience ( 68.88 ± 10.81 ) and academic failure luck (4.37 ± 2.21 ) than those having siblings ( (67.44 ± 10.18 ),(3.96 ± 2.12 ),t =2.15,3.02 ).Positive coping style,ncgative coping stylc,academic failure external attribution,academic success internal attribution,residence,and academic failure internal attribution had significant direct influence on resilience(β =16.435,- 4.919,- 0.344,0.308,1.396,- 0.182).ConclusionPositive coping styles and reasonable academic attribution can effectively predict great resilience of freshmen.

Objective To investigate the clinical correlative factors of malignant cystic pancreatic tumors.Methods The clinical data of 45 patients who received cystic pancreatic tumor resection at the General Hospital of Tianjin Medical University from May 2000 to May 2011 were retrospectively analyzed.All patients were divided into benign tumors + precancerous lesions group (35 patients) and malignant tumor group (10 patients).The clinical symptoms and imaging features of cystic pancreatic tumors were analyzed.All data were analyzed by chisquare test or Logistic regression analysis.Results Abdominal pain,jaundice,emaciation,nausea and vomiting were observed in 23 patients (51％),and 22 (49％) patients had no clinical symptoms.The clinical features of benign pancreatic cyst included pancreatic calcification and pancreatic divisum,while the clinical features of malignant pancreatic cystic tumors were nodules,swelling of lymph nodes,dilation of biliary and pancreatic duct.The results of univariate analysis showed that age ≥ 60 years,presence of symptoms,jaundice,emaciation,dilation of pancreatic duct were the correlative factors of malignant cystic pancreatic tumors ( x2 =4.220,4.294,4.645,7.705,4.645,P ＜ 0.05 ).The results of multiple logistic regression analysis found that age ≥60 years,dilation of pancreatic duct and presence of clinical symptoms were the correlative factors of malignant cystic pancreatic tumors ( OR =1.573,2.674,2.723,P ＜ 0.05).Conclusion Age≥60 years,dilation of pancreatic duct and presence of clinical symptoms are the correlative factors of malignant cystic pancreatic tumors.

Objective To analyze the short-term treatment effects and adverse effect on intermediate or advanced lung cancer by means of transbronchial arterial chemoembolization. Methods 68 patients were included in this study. First bronchial arteriography was performed to identify the tumor-feeding branch, and super-catheterize it. After CBP, EPI and 5-Fu was infused, embolization with gelatin sponge was carried out, chemoembolization was reperformed at 4~6 weeks intervals, 136 times of chemoembolization were done in 68 cases. Results The tumor blush of 51 patients stained obviously ,the feeding arteries with the second treatment decreased obviously. In 68 patients, the overall response rate was 82.3 %, the clinical beneficial rate was 97.0 %. The main side effect was myelosuppression, especially granulocytopenia, which was similar to that of conventional intravenous chemotherapy. No other severe adverse effect was seen. Conclusions Transbronchial arterial chemoembolization by selective tumor-feeding arteries catheterization has good short-term effect and clinic beneficial rate in the treatment of intermediate or advanced lung cancer, but the long-term treatment effects will be studied in the future.

Objective:To determine the distribution of CD4+CD25+FOXP3+ regulatory T cells (Treg) and Treg subsets in human colorectal carcinoma microenvironment and to explore their correlation with conventional clinico-pathological features.Methods:Frozen sections and Immunohistochemistry (IHC) were used to detect FOXP3+ Treg in fresh specimen collected from 42 patients with colorectal carcinoma.The number of FOXP3+ Treg was evaluated in terms of its association with clinico-pathological feature in tumor and peri-cancer tissue.Double staining was performed to determine the expression of ICOS and FOXP3.Results:The number of FOXP3+ Treg in the colorectal carcinoma (mean 24.1) was significantly higher than that in peri-cancer tissue (mean 0.7).A higher number of tumor infiltrating FOXP3+ Tregs was found in the patient groups with poor differentiation,lymphatic metastasis and non-distant metastasis as compared to the patient groups with well differentiation,non-lymphatic metastasis and distant metastasis.The percentage of FOXP3+ ICOS+ Treg was higher in colorectal carcinoma(81%) than that in peri-cancer tissue(10%).Conclusion:Increased FOXP3+ Treg may influence the occurrence and development of colorectal carcinoma.Our data support the hypothesis that tumor infiltrating FOXP3+ Tregs attenuate the immune response against cancer and suggest that strategy to overcome FOXP3+ Treg function may be beneficial in the treatment of human colorectal cancer.