Objective To investigate the changes in Doppler perfusion index(DPI)of the liver and the platelet derived-endothelial cell growth factor(PD-ECGF)in patients with liver metastasis from colorectal carcinoma by determination DPI and PD-ECGF mRNA and protein expression levels.Methods One hundred patients were enrolled,including 50colorectal carcinoma patients(22of whom with liver metastasis and 28patients without liver metastasis)and 50healthy subjects as control.For all the objects involved,hepatic perfusion was documented with color Doppler flow imaging,and the DPI(hepatic arterial/total liver blood supply)was computed.The expression levels of protein and mRNA of PD-ECGF in 50specimens of carcinoma and adjacent tissues of colorectal carcinoma were assayed by immunohistochemistry and real-time fluorescence quantitative PCR methods in patients with colorectal carcinoma.Results According to pathology and imaging results,the DPI of colorectal carcinoma patients with liver metastasis was significantly higher than that in healthy control group and non-liver metastasis group(P0.05).According to the results of immunohistochemistry and real-time fluorescence quantitative PCR,the expression levels of PD-ECGF protein and mRNA were significantly higher in colorectal carcinoma than that in adjacent tissues(P

Objective To propose an improved C-means segment method based on Gibbs random field accelerated by GPU.Methods The parallel computation of pixel shades was used to take the place of the classical point-by-point method of CPU.By this way,the efficiency was higher than merely using the CPU computation.Results The efficiency of computation was improved over 400%.The load of CPU was reduced and the effect of accelerator was obvious.Conclusion The improved C-means segment method based on Gibbs random field accelerated by GPU enhances the clinical application of image segmentation,the computer rate of which is improved distinctly and closely to real time.[Chinese Medical Equipment Journal,2008,29(2):6-9]

6cm,the recrudescenc rates when factors were male,mild or moderate malignant,stage of Dukes C,distance from tumor to anus 4～6cm were significantly higher(?2=4.53,P

Objective: To explore a simple method of cryptotia correction with good effect. Methods: 7 patients and 10 ears were operated on, using a supra-auricular V-Y flap. The auricular muscles′ adhesion with cartilage was sufficiently released, and the scapha cartilage was remodeled by a reverse suture. The flap was rotated to post-auricular to close the wound, and deepenen the auriculocephalic angle. Results: All patients′ ears nearly had a normal appearance and an obvious crus of helix. Patients could wear glasses or respirator easily, as their supra-auricular part was free from the skull. Our technique ensured all scars were hidden behind the ear or in the hair. No patients required revisional surgery. Conclusions This technique is a simple method , which has a good result , covert scar and low recurrence. We can use it for majority of patients with cryptotia.

MR diffusion tensor imaging (DT-MRI) is a new technology which reflects the direction of molecule diffusion better than traditional diffusion weighted imaging (DWI) does. DT-MRI has great advantages of showing the distribution of white matter fiber path and its spatial structures because the water molecules in brain white matters have the obvious anisotropic diffusion. This paper introduces the basics of diffusion tensor imaging, and reviews the visualization methods used with DT-MRI.
Brain ; pathology ; Brain Mapping ; methods ; Diffusion Magnetic Resonance Imaging ; methods ; trends ; Humans ; Image Enhancement ; methods ; Image Processing, Computer-Assisted ; methods

OBJECTIVEThis study aims to evaluate the bond strength of a self-adhesive resin cement to dentin by ethylene diamine tetraacetic acid (EDTA) and NaClO.

METHODSTwenty-seven freshly extracted non-carious human premolars were prepared to expose the buccal dentin and randomly divided into three groups: control group (A group), EDTA group (B group) and NaClO group (C group). All teeth were bonded to dentin using a self-adhesive resin cement after the teeth in the A group were processed with distilled water. The B and C group were processed with 3%EDTA and 1%NaClO, respectively. After 24 hours at 37 °C water, the shear bond strengths of the twenty-four specimens were measured. All statistical analysis was performed using SPSS 17.0 software package. Each fractured specimen was examined under dental microscope. Three new specimens were cut, and the morphologies of the cement-dentin interface were observed under scanning electron microscope (SEM).

RESULTSThe shear bond strength in the A group, B group and C group was (8.55±0.63), (8.47±0.56) and (12.97± 0.59) MPa, respectively. The difference between A group and B group was no statistically significant (P>0.05), whereas the difference between C group and B group (or A group) was statistically significant (P<0.05). SEM observation of the cement-dentin interface in the C group showed good adaptation, but resin tags were not observed. The other two groups showed poor bonding interface. Most of the fractured adhesive dentin surfaces exhibited cohesive failure in the A group and B group. All the fractured adhesive dentin surfaces exhibited cohesive failure in the C group.

CONCLUSION1% NaClO can increase the bond strength of self-adhesive resin cement to dentin, but 3%EDTA has no effect.

Adhesives ; Dental Bonding ; Dental Stress Analysis ; Dentin ; chemistry ; Dentin-Bonding Agents ; Detergents ; chemistry ; Humans ; Resin Cements

Objective To determine the baseline echocardiographic characteristics and the time course and degree of recovery of left ventricular (LV) systolic dysfunction in patients with tachycardia-induced cardiomyopathy ( TCM ) . Methods Seven hundred and fifteen patients received radiofrequency cather ablation ( RFCA ) for tachycardiarrhymias from July 2010 to July 2013 were screened in this study. Only 33 patients with reduced left ventricular ejection fraction (LVEF) (LVEF<50% and improved≥15%) were diagnosed with tachycardia-induced cardiomyopathy and were included in the study. Patients with early improvement (over 25%increase in LVEF at 1-week follow-up compared to the baseline ) were enrolled in the improved group , and the rest patients were enrolled in the improved group. All Patients received transthoracic echocardiography for LV size and function detection at 1 week and at 3,6,12 months follow-up. Results The average baseline of the LV end-diastolic diameter, and the LVEF were (55 ± 10.7)mm and (38 ± 4.6)%, repectively. Early improvement ( over 25%increase in LVEF at 1-week follow-up compared to the baseline ) in the improved group was observed in 16 patients. Patients with early improvement had higher LVEF at 12-month follow-up compared to the patients without early improvement [(69.2± 4.2)% vs (58.1 ± 6.9)%, P < 0.001]. Conclusions RFCA is proved to be a relatively safe and effecient treatment method. Atrial fibrillation related to TCM , rhythm control is superior to the rate control. The early improvement in LVEF may potentially predict the complete reversibility of LV systolic dysfunction.

OBJECTIVE To investigate the produce of extended-spectrum ?-lactamases(ESBLs) and the presence of genotype of the ?-lactamases-encoding genes in Klebsiella pneumoniae isolated from the 98th Hospital of PLA,Huzhou,Zhejiang Province,China.METHODS Twenty-five strains of K.pneumoniae were isolated from the inpatients between Sep 2005 and Apr 2006.ESBLs were tested by phenotypic confirmatory tests recommended by CLSI.Twenty-one kinds of ?-lactamases genes of blaTEM,blaSHV,blaLEN,blaOKP,blaCTX-M-1 group,blaCTX-M-2 group,blaCTX-M-9 group,blaOXA-1 group,blaOXA-2 group,blaOXA-10 group,blCARB,blaPER,blaVEB,blaGES,blaLAP,blaDHA,blaACT/MIR,blaCMY/MOX,blaFOX,blaCMY/LAT,and blaACC were analyzed by PCR and verified by DNA sequencing.RESULTS In 25 strains of K.pneumoniae,the positive,negative,and "uncertainty" rates of ESBLs were 56.0%,20.0%,and 24.0%,respectively.The positive rate of genes of blaTEM,blaSHV,blaCTX-M-1 group,blaOXA-10 group,blaLAP,and blaDHA were 80.0%,4.0%,4.0%,80.0%,4.0% and 32.0%,respectively.The 15 kinds of rest genes were all tested negative.The total positive rate of 21 kinds of ?-lactamases gene was 92.0%.Among them,the blaLAP-2 gene sequence of the HZ12593 strain has been registered in GenBank(GenBank Accession Number: EU529981).CONCLUSIONS There are higher rate of ESBLs-producing strains in K.pneumoniae isolated from the inpatients,and at least 6 kinds of ?-lactamases gene existed.Both genes of blaTEM and blaOXA-10 group are the most common genotypes.Carring blaDHA Gene may influence the result of phenotypic confirmatory test for ESBLs in K.pneumoniae.

Objective: This study aimed to present our clinical experience using a novel method for autologus costal cartilage framework fabricationin fully expansion technique ear reconstruction without skin graft. Methods: During Steptember 2016 to Steptember 2017, autologus costal cartilage framework with expansion technique were performed for 63 patients with microtia. Autologous costal cartilage was stacked up to form the structure and height, the natural appearance of auriculocephalic angle was achieved at the same stage, so the second-stage elevation of ear and postauricular skin graft was unnecessary. The delicate structure of the reconstructed ear and ideal bilateral symmetry were achieved by the optimized cartilage stack-up. Results: Three patients out of total 63 patients had cartilage frame work exposure, and were treated with superficial temporal fascia transfer combined with skin graft. The rest 60 patients had satisfied results. Follow-up ranged from 8 months to 18 months and 12 months follow-up was on average. The novel frame work fabrication method was based on the sufficient full pre-expansion procedure. The ideal structure, bilateral symmetry, and excellent definition of the framework, as well as the natural dorsal appearance of the reconstructed auricular were achieved. Conclusions The cartilage " stack-up" method is essential for the full expansion without skin grafts ear reconstruction technique. The combination of the cartilage " stack-up" framework fabrication and full pre-expansion provide a well-defined, well-projected and bilateral symmetrical ear.

ObjectiveTo explore the expression of N-cadherin and β-catenin mRNA in human brainstem and supratentorial gliomas. MethodsN-cadherin and β-catenin mRNA expression in 18 cases of brainstem gliomas and 18 cases of supratentorial gliomas tissues were detected with PT-PCR. Resultsβ-catenin mRNA expression was more in human brainstem gliomas than in supratentorial gliomas （t=2.255,P<0.05）, but was not significantly different of N-cadherin mRNA (P＞0.05). The expression of N-cadherin mRNA in human brainstem gliomas of grades Ⅰ～Ⅱ were less than those in human gliomas of grades Ⅲ～Ⅳ (t=2.711，P<0.05), but was not of β-catenin mRNA (P＞0.05). N-cadherin mRNA expression was positively correlated with the β-catenin mRNA expression in either brainstem gliomas or supratentorial gliomas （r=0.480，r=0.809 respectively, P<0.05）. ConclusionThe over expressions of N-cadherin and β-catenin may play an important role in the invasion and malignant progress of human brainstem gliomas.