Infectious mononucleosis associated with Epstein-Barr virus infection (EBV-IM) is a proliferative disease of acute monocyte - macrophage system caused by primary EBV infection. Currently, the incidence IM has increased,and the cases are more severe. The mechanism of specific cellular immune response, new treatments and vaccines are increasingly becoming the focus.

Objective To analyze the short-term treatment effects and adverse effect on intermediate or advanced lung cancer by means of transbronchial arterial chemoembolization. Methods 68 patients were included in this study. First bronchial arteriography was performed to identify the tumor-feeding branch, and super-catheterize it. After CBP, EPI and 5-Fu was infused, embolization with gelatin sponge was carried out, chemoembolization was reperformed at 4~6 weeks intervals, 136 times of chemoembolization were done in 68 cases. Results The tumor blush of 51 patients stained obviously ,the feeding arteries with the second treatment decreased obviously. In 68 patients, the overall response rate was 82.3 %, the clinical beneficial rate was 97.0 %. The main side effect was myelosuppression, especially granulocytopenia, which was similar to that of conventional intravenous chemotherapy. No other severe adverse effect was seen. Conclusions Transbronchial arterial chemoembolization by selective tumor-feeding arteries catheterization has good short-term effect and clinic beneficial rate in the treatment of intermediate or advanced lung cancer, but the long-term treatment effects will be studied in the future.

Objective To study the refining of Radix Sophorae Flavescentis (RSF) decoction by microfiltration combined with ultrafiltration method,and to compare the refining effect with that refined by alcohol sedimentation method.Methods HPLC was applied to analyze the content of oxymatrine,ultroviolet (UV) spectrophotometry was used to determine the content of total flavonoids in RSF decoction,and suitable concentration of NaOH solution for the detergent were chosen in order to wash the membrane.Results The refining effect is better and the recovery ratio of efficient components is higher in RSF decoction refined by microfiltration combined with ultrafiltration method than in those refined by alcohol sedimentation method.Microfiltration combined with ultrafiltration method is of simple operation and short period,and ultrafiltration membranes can be easily washed.Conclusion The microfiltration combined with ultrafiltration method can be used as the alternative for the traditional alcohol sedimentation method in refining of RSF decoction.

Objective: To study the refinement of general flavone of sophora flavescens decoction by macroporous resins combined with ceramic microfiltration membranes (MR-CM), and compare it with macroporous resin combined with alcohol-sedimentation (MR-AS). Methods: UV-spectrophotometry method was applied to analyze the content of general flavone of sophora flavescens. Results: The porous ratio and effect of eliminating impurity of general flavone of sophora flavescens decoction by MR-CM method was higher than those by MR-AS method, but the MR-CM method is of simple operation and short period.Conclusion: MR-CM method is better than alcohol-sedimentation-macroporous resin method in refining of general flavone of sophora flavescens decoction.

OBJECTIVEOptical coherence tomography images may be distorted by motion artifacts in dynamic imaging, so it may lead to misdiagnosis in clinical diagnosis. Motion artifacts correction has become an urgent issue in optical coherence tomography imaging.

METHODSFirstly, using the improved complex nonlinear diffusion preprocessing filtering reduced the noise of the sequence images, then using the image mass center aligned the distortion data in the Y direction, finally, using the method of deviation average corrected motion artifacts along the Y direction.

RESULTSAfter correction, the motion artifacts in the longitudinal 2D images and the 3D image disappeared, the surface of the 2D and the 3D image became more smooth, the structure between layers of the images got clear and distinct, retinal en-face single slice image was sharp, and the fundus tissue structure could be observed.

CONCLUSIONThe algorithm of correction makes the physical structure of the retinal display truly after motion artifacts correction.

The Epstein-Barr virus (EBV) is a gamma herpes virus associated with several types of malignancies. The EBV encodes viral microRNAs (miRNAs) that can target genes within cells. The EBV participates in signal transduction as well as the proliferation and differentiation of cells. How the target genes and functions of EBV-encoded miRNAs contribute to the pathogenesis of EBV is an important research topic. Some target genes have been validated since EBV-encoded miRNAs were discovered and, in this article, we summarize them and their functions.
Animals ; Epstein-Barr Virus Infections ; genetics ; metabolism ; virology ; Herpesvirus 4, Human ; genetics ; physiology ; Humans ; MicroRNAs ; genetics ; metabolism ; RNA, Viral ; genetics ; metabolism

OBJECTIVEUsing the algorithm on the layered structure of the retina and quantitative analysis of the automatic segmentation technique is the key to the early diagnosis of glaucoma and other retinopathy on optical coherence tomography. Existing methods require high quality image and have low reliability. This paper used the improved complex nonlinear diffuse filtering and other methods to solve this problem.

METHODSThis paper includes algorithm such as automatic threshold, improved complex nonlinear diffusion filtering, morphological operations and peak detection. Use the method for the segmentation of 20 retinal layers images which acquired on the self-builder OCT system, the boundary of inner limiting membrane(ILM), outer nuclear layer(ONL), the photoreceptor segments(IS/ OS) and the RPE_ChCap layer are detected accurately. At last, the photoreceptor layer thickness is measured.

RESULTSThe results of segmentation and measurement are good corresponded with expert manual segmentation and measurements, retinal foveal measurements data is consistent with Zeiss Stratus OCT central retinal thickness 212+/-20 micro m.

CONCLUSIONThe algorithm proposed is prospective applied to clinical diagnosis of retinal diseases.

Objective:To investigate whether the increased plasma fibrinogen level is an independent predictor of cardiogenic embolism in patients with nonvalvular atrial fibrillation (NVAF).Methods:Ninety-eight patients with NVAF hospitalized for cardiac causes were evaluated,and 24 of them had a history of ischemic stroke.The relationship between fasting plasma fibrinogen level and ischemic stroke were analyzed.Results:After adjusting traditional cardiovascular risk factors.the patients with NVAF who had the history of ischemic stroke were significantly older than those without(mean [SD] age 73.6 [8.4]vs.69.1[10.3] years,P=0.017).1he prevalence of hypertension was significantly higher(83.3%va.66.2%,P=0.011).The proportion of patients with left ventricular systolic dysfunction was significantly higher(63.1% vs.45.9%,P=0.006),and plasma fibrinogen level increased significantly (mean [SD] 365 [108]vs.312[82] mg/dL,P=0.008).Conclusions:Plasma fibrinogen level is independently associated with a history of ischemic stroke in older patients with NVAF hospitalized for cardiac causes.

Objective To develop a method for the determination of ganciclover in human plasma by RPHPLC.Methods Plasma containing ganciclover was extracted with methanol and methylene chloride,qualitative and quantitative analysis was carried out directly.Working curve,linear range,recovery,precision and so on was obtained according to the sample pre-processing method and analysis state.The HPLC method has been taken to investigate the plasma concentration of ganciclover for 12 volunteers.Results The relationship of the peak area of ganciclover concentration in plasma linear within the range of 0.05 μg/mL～1.60 μg/mL(r=0.9999).The lowest detection limit was 0.01 μg/mL(S/N≥13).The intra and inter-day RSD were less than 5.1%respectively.The recovery is about 90.0%～95.4%.Conclusion The established method in the article was shown to be sensitive,accurate and simple for the determination of ganciclover level.It is suitable for clinical detection of ganciclover and forensic medicine and toxicology analysis.

Objective To reduce the error caused by the non-linearity of headphone and device via the design of calibration algorithm for acoustic immittance.Methods Values of calibration chambers with different volumes (0.5,2.0 and 5.0 ml) were measured using the digital detection system.Then,the calibration data tables,for linearity calibration with inputting the values of acoustic immittance,were produced by means of processing the signal of acoustic immittance using the digital calibration algorithm based on the values from the calibration chambers,Results With the low frequency probe tone measurement,it was confirmed that the range of error among various calibration chambers with different volumes was 1％.Conclusion The calibration algorithm enables the improving of the detection accuracy and provides the reliable data for further clinical application.