Objective To observe the effect ofXuebijing injection on vascular endothelial function and inflammatory factor in the patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods A total of 100 patients with AECOPD were randomly divided into the study and control groups, 50 patients in each group. The control group were given the conventional therapy. The study group were given the Xuebijing injection on the basis of the control group. Both The changes of lung function, vascular endothelial function and inflammatory factor were observed.Results The expression of FVC (3.23 ± 0.83 Lvs. 2.79 ± 0.71 L,t=1.786), PEF (5.36 ± 0.51 L/svs. 4.79 ± 0.46 L/s,t=1.904), FEV1/FVC (59.21 ± 4.38vs. 54.94 ± 4.29,t=1.882), NO (3.84 ± 0.81 ng/mlvs.2.97 ± 0.62 ng/ml,t=1.869) increased significantly in the study group than those in the control group after treatenmt (P<0.05). The VEGF (0.14 ± 0.04 ng/ml vs.0.17 ± 0.05 ng/ml, t=2.097), TNF-a (0.11 ± 0.03 ng/ml vs.0.14 ± 0.02 ng/ml,t=1.903), IL-13 (107.27 ± 44.37 ng/mlvs. 136.54 ± 48.32 ng/ml,t=2.005) in the study group decreased significantly than those in the control group (P<0.05). ConclusionsXuebijing injection could significantly decrease inflammatory factor,and obviously improve vascular endothelial function and lung function,the clinical curative effect is distinct.

This article discussed the necessity and method of setting up a comparative imaging medicine course, which is considered to be valuable for the future development and creativity of graduate students majored in nuclear medicine and imaging medicine.

Objective To explore variance and resource of intracellular free Ca2+ and extracellular arachidonic acid (AA) in different types of passage cells during the invasion of T. gondii. Methods The variance and resource of extracellular AA and intraceUular free Caz+ of Vero and J774A. 1 cells during the invasion of T. gondii were detected by multi-purpose scintillation counter and laser scanning confocal microscope. Data were analyzed using Chi-square test and t test. Results The intracellular free Ca2+ levels in J774A. 1 and Vero cells were both increased after T. gondii infection. The maximal changes of fluorescence intensity were (1 219.7±58.4)% (P＜0.01) and (356.3±23.6)% (P＜0.05), respectively.The increase of intracellular Ca2+ level in Vero cell was mostly from the release of intracellular Ca2+ store.And the Ca2+ increase in J774A. 1 cell was from both the release of intracellular Ca2+ store and extracellular Ca2+ influx. Extracellular AA levels were significantly increased in both Vero and J774A. 1 cells after T.gondii infection 5.02 and 8. 44 times respectively (t= 3. 124, t = 3. 852, P＜0.01). The AA elevations could be significantly inhibited by phospholipase A2 (PLA2) inhibitor pretreating T. gondii. Conclusions The phospholipase C of phagocytic host cell and PLA2 of T. gondii are activated by T. gondii infection,which results in the increase of intracellular free Ca2+ and extraeellular AA level. Combined actions of Ca2+and AA play a major role in the invasion of T. gondii to host cell. While only PLA2 of T. gondii may be activated in nonphagoeytic host cell.

Objective To selectively resect the lymph nodes in the endometrial cancer by risk factors evaluation pre-and intra-operation.Methods Three hundred and seventy-four cases who in the Obstetric and Gynecology Hospital Affiliated to Fudan University from Oct.2010 and Dec.2012 were clinically diagnosed as stage Ⅰ pre-and intra-operation,grouped as one (staged Ⅰ A,294 cases) and two (staged Ⅰ B,80 cases),and the situation of lymph nodes metastasis and the related risk factors were analyzed.Results (1) Lymph nodes metastasis were positive in 6 cases in group one (2.0％) and 12 cases in group two(15.0％),and the difference was significant between two groups (x2 =23.054,P =0.000).(2) University analyses showed that pathologic type,grade,myometrial invasion,lymphovascular invasion and cervical gland invastion were the risk factors contributed to the lymphatic metastasis in early staged endometrial cancer (P＜ 0.05).Multivariate analysis showed that myometrial invasion was independent risk factor affecting early metastasis of lymph node in endometrial carcinoma (regression coefficient =2.339,standard error =0.585,OR =10.897,95％ CI 3.273 ～ 32.367,P =0.000).(3)Based on the gynecology oncology group test and risk factors that recent used in different countries and clinical centers,superficial myometrial layer invasion,G2,age＞ 60 years,tumor ≥ 2 cm in diameter as the medium risk factors,and deep myometrial layer invasion,G3,lower portion of the uterus or cervical gland invasion as high risk factors,and patients with two or more medium risk factor (106 cases) and with one or more high risk factors(138 cases) were inclined to develop lymph node metastasis((5.66％ (6/ 106),0(0/130),P =0.04;8.70％ (12/138),2.54％ (6/236),P =0.007).(4) Two hundred and thirteen patients were found with superficial layer invasion by frozen section,and 11 of them were upgraded to Ⅰ B by postoperative pathologic examination.Interestingly,all of the 11 cases were accompanied by two medium or one of high risk factor.Conclusion Low incidence of lymph nodes involvement is found in stage Ⅰ endometrial cancer,especial stage Ⅰ A.Myometrial invasion is closely linked to lymph node metastasis,thus,lymphadenectomy should routinely be carried out in endmetrial cancer diagnosed as Ⅰ B whether pre-or intraoperation.As in stage Ⅰ A,unless two medium and one high risk factors are found.

Objective To study a multiple frequency tympanometry on ear system. Methods Based on the original low frequency and single ingredient SY-IA tympanometry, the study mainly focused on such aspects as multiple frequency probe tone circuit, constant pressure sound control system, micropressor control system and multiple frequency acoustic immitance & phase angle detection circuit. Results Multiple frequency tympanometry on ear system could detect multiple frequency acoustic immitance and phase angle for normal ear, tympanum loosed ear and ossicular chain break ear under different probe tone. Conclusion Experiments show that multiple frequency tympanometry on ear system based on ARM is helpful to the diagnosis of pathological changes which are insensitive to single low frequency tympanometry.

ObjectiveTo explore the relationship among resilience,attribution,and coping style for freshmen.Methods 977 freshmen were surveyed with Connor-Davidson Resilience Scale,Multidimensional-Multiattributional Causality Scale,and Simplified Coping Style Questionnaire,then compared different freshmen's resilience,attribution,and coping style,and analyzed their relationship.ResultsThe city freshmen showed significant higher resilience(68.88 ± 10.57 ),academic external attribution (21.14 ± 6.01 ),and interpersonal external attribution ( 22.87 ± 6.42 ) than rural freshmen ( ( 67.51 ± 10.43 ),( 19.81 ± 5.88 ),( 21.86 ± 5.88 ) respectively,t =2.03,3.48,2.57).Vocational freshmen showed significant higher academic internal attribution ( 28.68 ± 5.30 )and interpersonal internal attribution (26.81 ± 5.82) than undergraduate freshmen ( ( 27.61 ± 5.47 ),( 25.53 ±5.92),t =3.00,3.30).The only children showed significant higher resilience ( 68.88 ± 10.81 ) and academic failure luck (4.37 ± 2.21 ) than those having siblings ( (67.44 ± 10.18 ),(3.96 ± 2.12 ),t =2.15,3.02 ).Positive coping style,ncgative coping stylc,academic failure external attribution,academic success internal attribution,residence,and academic failure internal attribution had significant direct influence on resilience(β =16.435,- 4.919,- 0.344,0.308,1.396,- 0.182).ConclusionPositive coping styles and reasonable academic attribution can effectively predict great resilience of freshmen.

Objective To keep the intensity of sound constant and reduce the effect of ear canal difference on detection process.Methods Real-time detection of the probe tone in ear canal was carried out and by control ling the output circuit of the probe tone through the digital signal compensation algorithm,to realize real-time digital tympanometry of ear system and the function of constant pressure control.Results The results showed that the probe tone remained constant at 226 Hz for the measurement of middle ear and tympanometry was accurate.Conclusion The study simplified system configuration and provides basis for the clinical application.

Objective To compare the efficacies of several commonly-used penetrating needling methods in treating post-stroke strephenopodia. Method Eighty-three patients with post-stroke strephenopodia were randomized into four groups, a control group intervened by conventional needling method (n=20), and the rest three groups were additionally intervened by different penetrating needling methods: penetrating group 1 [Qiuxu (GB40) towards Zhaohai (KI6), n=21], penetrating group 2 [Taibai (SP3) towards Shugu (BL65), n=21], and penetrating group 3 [Jiaoxin (KI8) towards Fuyang (BL59), n=21], to compare their effects on the function of ankle and lower extremity. Result The penetrating needling methods and conventional needling method all can significantly improve the function of ankle in patients with post-stroke strephenopodia (P<0.01). The effectiveness of penetrating group 1 was more significant than that of the rest groups (P<0.01), the effectiveness of penetrating group 2 was more significant than that of penetrating group 3 and the control group (P<0.05,P<0.01), and there was no significant difference in comparing the effectiveness between penetrating group 3 and the control group (P > 0.05). The penetrating needling methods and conventional needling method all can significantly improve the motor function of lower extremity in patients with post-stroke strephenopodia (P<0.01). The effectiveness of penetrating group 1 was more significant than that of the other groups (P<0.01), the efficacies of penetrating group 2 and 3 were more significant than that of the control (P<0.01), and there was no significant difference in comparing the effectiveness between penetrating group 2 and 3 (P>0.05). Conclusion Needling from Qiuxu towards Zhaohai and from Taibai towards Shugu can produce significant effects in improving ankle function and the motor function of lower extremity in patients with post-stroke strephenopodia, while needling from Jiaoxin to Fuyang doesn’t have notable advantage compared to the other penetrating needling methods and conventional needling method.

Objective To investigate the correlation between PaCO2 and cerebral blood flow (CBF), cerebral metabolism of oxygen (CMRO2), glucose (CMRglu) and lactate (CMRlact) and intracranial pressure during intracranial surgery. Methods Twenty ASA Ⅰ - Ⅱ patients (6 male, 6 female), aged between 26-54yr, weighing (65 ? 11) kg scheduled for elective intracranial surgery were studied. The patients were unpremedicated. Before general anesthesia radial artery was cannulated and a catheter was inserted into internal jugular vein and advanced cranially until jugular bulb. Lumber puncture was performed at L3-4 and a catheter was inserted into subarachnoid space for 3 cm, for pressure monitoring and CSF sampling. Anesthesia was induced with diazepam 10mg, fentanyl 3-4?g?kg-1, propofol 2mg?kg-1 and vecuronium 0.08mg?kg-1 iv. The patients were mechanically ventilated with a mixture of oxygen and argon (O2 : argon = 3 : 1) after tracheal intubation. Anesthesia was maintained with sevoflurane and intermittent iv boluses of vecuronium. Arterial and cerebral venous blood gases, glucose and lactate levels, CBF, ICP and CSF level of lactate were determined before anesthesia when patients were awake(Ⅰ) and during anesthesia when PETCO2 = 40, 30, 20 mm Hg (Ⅱ,Ⅲ,Ⅳ). CBF was measured by modified Kety-Schmidt inert gas saturation technique with argon. CMRO2 and CMRglu were calculated based on the difference in their arterial -cerebral venous blood levels. Results At PETCO2 20mm Hg (Ⅳ) CBF decreased by 57.75% and CMRO2 by 58.70% as compared with the baseline; CMRglu decreased by 46.93% as compared with the baseline. There was no significant change in lactate level, jugular venous blood O2 saturation and pH. ICP decreased from (22.14 ? 7.88)mm Hg( Ⅰ) to (17.57?5.03)mm Hg( Ⅱ ),(13.43?4.89)mm Hg(Ⅲ) and (10.00? 2.31)mm Hg(Ⅳ) and the differences were significant. All measurements were done when MAP and HR were stable. PET CO2 was (10? 2) mm Hg lower than PaCO2 . Conclusions Cerebral blood flow, cerebral oxygen and glucose metabolism and intracranial pressure change with changes in PET CO2 . Cerebral vascular reactivity to CO2 is not impaired by 1.3 MAC sevoflurane. Mild hypocapnia is necessary during neurosurgery.

Objective　To discuss the clinical value of 18　F-FDG PET imaging in postoperative and postradiotherapeutic intracranial glioma during follow up study. Methods　18F-FDG PET imaging in 16 cases of postoperative and postradiotherapeutic intracranial glioma was compared with CT and/or MRI. Results　Contrast CT or MRI showed prominent irregular circular or nodular enhancement in 14 of 16 cases (64%),can not distinguished from postopertative changes, radioinjury, tumor residue or recurrence.Among them, 9 cases showed tumor residue or recurrence based on significantly increased FDG uptake, 5 were confirmed by pathologic study. The other 5 cases of cerebral necrosis and 2 cases with postoperative cerebral malacia demonstrated FDG uptake defects. Conclusions　18F-FDG PET imaging has significant dominance in characterizing lesions for differentiating residue or recurrent disease from radioinjury in intracranial glioma. Combined with CT and MRI can provide both anatomical and functional informations.