OBJECTIVE: To investigate and analyze the changes of postmortem human biochemical indexes. METHODS: Subclavian venous blood samples were collected from 81 cases of traffic fatalities. Thirteen blood biochemical indexes including liver function (ALT, AST, TBIL and DBIL), renal function (UA and Cr), cardiac function (CK, CK-MB and LDH), electrolytes (K+, Na+ and Cl-), and glucose (GLU) were tested by Roche cobas c311 automatic biochemical analyzer. The descriptive analysis was made by SPSS 17.0 statistical software. RESULTS: The values of ALT, AST, CK, CK-MB, LDH and K+ were higher than normal reference values with more fluctuations. The values of TBIL, DBIL, UA, Cr, Na+, Cl- and GLU were relatively stable with less fluctuations. CONCLUSION The postmortem human blood biochemical indexes of liver function, renal function, cardiac function, electrolytes and glucose could be affected by the factors, especially hemolysis and autolysis. The biochemical indexes, particularly enzymes, increased significantly with higher standard deviation.
Accidents, Traffic/mortality* ; Autopsy ; Blood Chemical Analysis/methods* ; Heart Function Tests ; Humans ; Kidney Function Tests ; Liver Function Tests ; Reference Values

Carcinoma, Hepatocellular ; physiopathology ; Humans ; Liver ; physiopathology ; Liver Function Tests ; methods ; Liver Neoplasms ; physiopathology ; Preoperative Care

This paper reviews metabolomics/metabonomics technologies used in digestive disease clinical diagnosis, summarizes mainly two aspects for using metabolomic methods in current diagnosis for gastrointestinal disease, i.e. the one used for diagnosis of gastrointestinal malignancy, the other improving diagnosis of disease which can not be easily distinguished by traditional clinical methods. The future use of the metabonomics in clinic is also analyzed in the present paper.
Gastrointestinal Diseases ; diagnosis ; Humans ; Liver Function Tests ; methods ; Metabolome ; physiology ; Metabolomics ; methods ; Stomach Neoplasms ; diagnosis

Adult ; Aged ; Female ; Glucagon ; Glucose Tolerance Test ; Humans ; Liver ; physiopathology ; Liver Cirrhosis ; physiopathology ; Liver Function Tests ; methods ; Male ; Middle Aged

Humans ; Liver Function Tests ; methods ; Technetium Tc 99m Aggregated Albumin ; Technetium Tc 99m Pentetate

Risks persists during liver resection in the patients with cirrhosis. Routine examinations usually are unable to predict the morbidity and mortality following surgery. Evaluation approaches of liver function, including aminotransferase, bilirubin, albumin, prothrombin time, Child-Pugh classification, and indocyanine green (ICG) test, currently remain the most important method to determine the prognosis of liver resection, especially in cirrhotic patients. However, all these approaches have their weakness. Some new methods, including non-invasive ICG test, liver function evaluation by hepatic imaging, and remnant hepatic function test, are awaiting further investigation. This article reviews the current status and future prospect in the assessment of liver functions.
Hepatectomy ; Humans ; Indocyanine Green ; Liver Cirrhosis ; diagnosis ; surgery ; Liver Function Tests ; methods ; Magnetic Resonance Imaging ; Radionuclide Imaging ; Risk Assessment

Animals ; Breath Tests ; methods ; Carbon Isotopes ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Chemical and Drug Induced Liver Injury ; etiology ; physiopathology ; Liver Function Tests ; methods ; Male ; Phenylalanine ; Rats ; Rats, Sprague-Dawley

Animals ; Animals, Newborn ; Bifidobacterium ; growth & development ; metabolism ; Gastrointestinal Tract ; microbiology ; Liver Function Tests ; Parenteral Nutrition ; methods ; Rabbits

OBJECTIVETo evaluate the safety of donors of right lobe graft.

METHODSWe retrospectively studied 13 living donors of right lobe graft from January 2002 to June 2005. The right lobe grafts were obtained by transecting the liver on the right side of the middle hepatic vein. Liver transection was done by using an ultrasonic dissector without inflow vascular occlusion. The standard liver volume and the ratio of left lobe volume to the standard liver volume were calculated.

RESULTSThe mean blood loss was 490 ml. The mean blood transfusion was 440 ml. In the perioperative period the mean albumin administered was 85 g. One donor had portal vein trifurcation, two had a right posterior bile duct and a right anterior bile duct draining into the left bile duct, respectively. One had bile ducts from left lateral and left internal segment and right duct draining into common hepatic duct. On postoperative day 1 the donors' liver functions were found impaired to some extent, but all the indices rapidly returned to the normal level at the end of the first week. Postoperative complications included 1 case of abdominal bleeding, 2 wound steatosis and 1 chyle leak. There was no donor mortality. All donors are well and have returned to their previous occupations.

CONCLUSIONSThe donation of right lobe graft for adult living donor liver transplantation is safe provided that the patency of the remnant hepatic vasculature and bile duct is ensured, the volume of the remnant liver exceeds 30% of the total liver volume, and there is no injury to the remnant liver.

Adult ; Female ; Follow-Up Studies ; Humans ; Liver ; blood supply ; surgery ; Liver Function Tests ; Liver Transplantation ; methods ; Living Donors ; Male ; Middle Aged ; Postoperative Complications ; Retrospective Studies ; Transplantation, Homologous

Acetamides ; Aminopyrine ; Animals ; Breath Tests ; methods ; Carbon Isotopes ; Chemical and Drug Induced Liver Injury ; diagnosis ; pathology ; physiopathology ; Humans ; Liver ; injuries ; pathology ; physiopathology ; Liver Cirrhosis ; diagnosis ; pathology ; physiopathology ; Liver Diseases ; diagnosis ; pathology ; physiopathology ; Liver Function Tests ; Phenylalanine ; Prognosis