Toxocariasis is a helminozoonosis caused by the infection of a human host by the larva of Toxocara canis and Toxocara catis, which are common nematodes in dogs and cats, and occurs more frequently in school age children. Toxocariasis shows variable clinical manifestations including hepatomegaly, bronchial asthma, uveitis, central nervous system symptoms with peripheral eosinophilia and an increased total serum IgE level. However a hepatic abscess caused by toxocara infection in adults is rare. We experienced a case of heavy alcoholic patients with a hepatic eosinophilic abscess caused by toxocara infestation, which was confirmed by microscopic examination of liver biopsy, enzyme-linked immunosorbent assay test, abdominal CT and the ultrasonography findings.
Adult ; Animals ; Humans ; Liver Abscess/*diagnosis/*parasitology/ultrasonography ; Male ; Tomography, X-Ray Computed ; Toxocara canis/*isolation & purification ; Toxocariasis/*diagnosis/parasitology/pathology

Clonorchiasis is caused by a chronic infestation of liver flukes, Clonorchis sinensis, and these reside mainly in the medium- and small-sized intrahepatic bile ducts. Therefore, diffuse, uniform, minimal or mild dilatation of these bile ducts, particularly in the periphery, without dilatation of the extrahepatic bile duct is the typical finding on several imaging modalities. We report here on the CT findings of an unusual case of hepatic parasitic abscess that was caused by clonorchiasis; this malady mimicked cholangiocarcinoma, and there was no dilatation of the intrahepatic bile ducts.
Tomography, X-Ray Computed/*methods ; Middle Aged ; Male ; Liver Abscess/*parasitology/*radiography/surgery ; Humans ; Diagnosis, Differential ; Clonorchiasis/*radiography/surgery ; Animals

No abstract available.
Albendazole/therapeutic use ; Animals ; Antiprotozoal Agents/therapeutic use ; Enzyme-Linked Immunosorbent Assay ; Eosinophilia/*diagnosis ; Humans ; Liver Abscess/*diagnosis/parasitology ; Male ; Middle Aged ; Tomography, X-Ray Computed ; Toxocara canis/*isolation & purification ; Toxocariasis/*diagnosis/drug therapy/parasitology

OBJECTIVETo compare the efficacy of three different tissue stains, namely haematoxylin and eosin (H&E), periodic-acid Schiff (PAS) and immunohistochemical (IHC) stains for detection of Entamoeba histolytica (E. histolytica) trophozoites in abscessed liver tissues of hamster.

METHODSAmoebic liver abscess was experimentally induced in a hamster by injecting 1 × 10(6) of axenically cultured virulent E. histolytica trophozoites (HM1-IMSS strain) into the portal vein. After a week post-inoculation, the hamster was sacrificed and the liver tissue sections were stained with H&E, PAS and IHC stains to detect the amoebic trophozoite.

RESULTSThe three stains revealed tissue necrosis and amoebic trophozoites, but with varying clarity. H&E and PAS stained the trophozoites pink and magenta, respectively, however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology. On the other hand, IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.

CONCLUSIONSIt can be concluded that out of the three stains, IHC is the best for identification of E. histolytica trophozoites in tissue sections.

Animals ; Disease Models, Animal ; Entamoeba histolytica ; cytology ; isolation & purification ; Histocytochemistry ; methods ; Immunohistochemistry ; methods ; Liver Abscess, Amebic ; diagnosis ; pathology ; Male ; Mesocricetus ; Microscopy ; Parasitology ; methods ; Staining and Labeling ; methods ; Trophozoites ; cytology