No Abstract available.
Toxocariasis/*diagnosis ; *Toxocara canis ; Lung/parasitology ; Liver/parasitology ; Korea/epidemiology ; Hypereosinophilic Syndrome/epidemiology/*parasitology ; Humans ; Cookery ; Animals

A patient with multiple-organ echinococcosis suffered from liver echinococcosis,lung echinococcosis,and pelvic echinococcosis successively in the past three decades.From the first operation at 19 years-old,she underwent operations several times due to the recurrence of multiple organ involvement.Echinococcosis is a zoonotic disease.Although the liver usually is the primary site,the disease can also invade many other organs.Diagnosis is typically based on disease history and imaging findings.Thorough removal of the lesions during the first operation is particularly important.Comprehensive evaluations and multi-disciplinary team are helpful in the treatment of patients with multiple organ invasion.
Adult ; Diagnostic Imaging ; Echinococcosis/surgery* ; Female ; Humans ; Liver/parasitology* ; Lung/parasitology* ; Pelvis/physiopathology* ; Young Adult

From July 2008 to June 2009, livers of the swamp eels (Monopterus alba) were investigated for advanced third-stage larvae (AL3) of Gnathostoma spinigerum. Results revealed that 10.2% (106/1,037) and 20.4% (78/383) of farmed eels from Aranyaprathet District, Sa Kaeo Province and those of wild-caught eels obtained from a market in Min Buri District of Bangkok, Thailand were infected, respectively. The prevalence was high during the rainy and winter seasons. The infection rate abruptly decreased in the beginning of summer. The highest infection rate (13.7%) was observed in September and absence of infection (0%) in March-April in the farmed eels. Whereas, in the wild-caught eels, the highest rate (30.7%) was observed in November, and the rate decreased to the lowest at 6.3% in March. The average no. (mean+/-SE) of AL3 per investigated liver in farmed eels (1.1+/-0.2) was significantly lower (P=0.040) than those in the caught eels (0.2+/-0.03). In addition, the intensity of AL3 recovered from each infected liver varied from 1 to 18 (2.3+/-0.3) in the farmed eels and from 1 to 47 (6.3+/-1.2) in the caught eels, respectively. The AL3 intensity showed significant difference (P=0.011) between these 2 different sources of eels. This is the first observation that farmed eels showed positive findings of G. spinigerum infective larvae. This may affect the standard farming of the culture farm and also present a risk of consuming undercooked eels from the wild-caught and farmed eels.
Animals ; Aquaculture ; Fish Diseases/*epidemiology/*parasitology ; Gnathostoma/*isolation & purification ; Gnathostomiasis/epidemiology/parasitology/*veterinary ; Larva ; Liver/parasitology ; Parasite Load ; Prevalence ; Seasons ; Smegmamorpha/*parasitology ; Thailand/epidemiology

A study on the ultrastructures of the prostate gland of Clonorchis sinensis was conducted. The presence of the prostate gland in this fluke has not been known up to present time. Authors observed the ejaculatory duct epithelium including its surrounding parenchyma and found the prostate gland with the help of the electron microscope. The prostate gland was consisted of numerous unicellular glands grouped around the ejaculatory duct. The individual cell was lobulated, tapering in the direction of the ejaculatory duct. The secreting ducts of the gland penetrated into the ejaculatory duct through the muscular layer and the basement membrane, and finally opened to the lumen of the ejaculatory duct. The secreting duct had single layered microtubules along the inner wall of the duct. The secretory bodies produced by the prostate gland seemed to be moved to the lumen of the ejaculatory duct through the secreting duct. The prostate gland of this fluke was less developed than that of Fasciola hepatica but the basic structures were quite similar. There were well-developed lamellae in the epithelia of all ducts concerning passage of spermatozoa from the testes to the male genital opening.
parasitology-helminth-trematoda ; Clonorchis sinensis ; electronmicroscopy ; prostatic gland ; rat ; liver

Total 9 cases of extraintestinal amoebiasis, amebic liver abscess and amebic lung abscess, were treated with metronidazole. The diagnosis and progress check were done by (1) stool examination, (2) size and pain of liver, (3) hematological examination, (4) urinanalysis, (5) X-ray or liver scanning (6) examination of aspirated pus from liver and (7) immobilization test of E. histolytica. Results are summarized as follows: Amebic hepatitis: Metronidazole was given to 3 cases with daily dose of 0.9-1.2 gm for 3-7 days. Clinical symptoms were completely restored by the treatment. Amebic lung abscess: One case was treated with metronidazole for 3 days with the daily dose 1.2 gm. X-ray findings showed remarkable improvement by the treatment. An additional administration of the drug for 6 days resulted complete resolution of the pneumonic changes. Amebic liver abscess: Four cases were treated with metronidazole. The daily doses were 0.4-2.4 gm and continued for 2-10 days. Aspiration of the abscess for 1-10 times or continuous drainage of pus were performed during the course supplementarily. All cases were recovered to normal by the combining treatment. No remarkable side effect was noticed by the drug administration, and no relapse was experienced up to date.
parasitology-protozoa-Entamoeba histolytica ; chemotherapy ; metronidazole ; liver ; lung ; amoebiasis

The present study was carried out to examine the effect of "Cortisone" on the worm burden migrated in the organs, and granulomatous change in the liver of mice infected with Toxocara canis eggs. Daily does of 0.5 mg "Cortisone" was administrated subcutaneously to Toxocara-infected mice with different schedule. The results are summarized as follows: More number of Toxocara larvae migrated into the muscle tissue, especially into the carcass through the wall of the large intestine and higher distribution rate and longer persistence of worms were also recognized in the carcass of the experimental group than in the control. Cortisone administration resulted the inhibition of granuloma formation, as well as the resolution of granuloma in the liver. However, the discontinuation of the administration induced the reccurence of heavier grade of inflammation. In non-cortisone treated group (control), cellular infiltration appeared around the worms in the liver on the 10th day of the infection, and typical granulomatosis was followed on the 20th day. As in above results, it is considered cortisone has remarkable anti-inflammatory and immunosuppressive effects in mice infected with T.canis, but histopathological changes on the granuloma formation might be related closely with the duration of Toxocara infection, dosage, duration and timing of cortisone administration.
parasitology-helminth-nematoda-Toxocara canis ; liver ; mouse ; cortisone

Although Vietnam has a high risk of fishborne zoonotic trematode (FZT) infections for humans, little information exists on the epidemiology of these infections in the country's fish. Because of the importance of cultured catfish and snakehead production in An Giang province, a major production area in the Mekong Delta of Vietnam, a survey for FZTs was carried out in randomly selected fish farms between June 2005 and March 2006. For comparison, wild fish from the same area were also surveyed. A total of 852 cultured fish from 4 districts were collected and examined by pepsin digestion to determine their FZT infection status. In Tra catfish, the prevalence of all types of metacercariae was 2.6%, of which the prevalence of Haplorchis pumilio was 0.7%. The overall prevalence of metacercariae in wild fish was 30.6%, of which 10.3% harbored zoonotic species: H. pumilio (2.8%) and Procerovum sp. (5.6%). The prevalence of Opisthorchis metacercariae, which were diagnosed as O. viverrini, was 1.9%. No metacercariae were found in cultured snakehead fish, although wild-caught snakehead fish had a FZT prevalence of 10.3%: 5.1% were O. viverrini; 2.6% H. pumilio; and 2.6% were Procerovum sp. These are the first reports of H. pumilio, Procerovum sp., and O. viverrini metacercariae in Vietnamese fish. These results indicate that consumption of improperly prepared fish represents a significant risk of acquiring FZTs in this south Vietnam region.
Animals ; Aquaculture ; Fish Diseases/*epidemiology/parasitology ; Fishes ; Intestinal Diseases, Parasitic/epidemiology/parasitology/*veterinary ; Liver Diseases, Parasitic/epidemiology/parasitology/*veterinary ; Prevalence ; Trematoda/growth & development/*isolation & purification ; Trematode Infections/epidemiology/parasitology/*veterinary ; Vietnam/epidemiology ; Zoonoses/*epidemiology/parasitology

Although Vietnam has a high risk of fishborne zoonotic trematode (FZT) infections for humans, little information exists on the epidemiology of these infections in the country's fish. Because of the importance of cultured catfish and snakehead production in An Giang province, a major production area in the Mekong Delta of Vietnam, a survey for FZTs was carried out in randomly selected fish farms between June 2005 and March 2006. For comparison, wild fish from the same area were also surveyed. A total of 852 cultured fish from 4 districts were collected and examined by pepsin digestion to determine their FZT infection status. In Tra catfish, the prevalence of all types of metacercariae was 2.6%, of which the prevalence of Haplorchis pumilio was 0.7%. The overall prevalence of metacercariae in wild fish was 30.6%, of which 10.3% harbored zoonotic species: H. pumilio (2.8%) and Procerovum sp. (5.6%). The prevalence of Opisthorchis metacercariae, which were diagnosed as O. viverrini, was 1.9%. No metacercariae were found in cultured snakehead fish, although wild-caught snakehead fish had a FZT prevalence of 10.3%: 5.1% were O. viverrini; 2.6% H. pumilio; and 2.6% were Procerovum sp. These are the first reports of H. pumilio, Procerovum sp., and O. viverrini metacercariae in Vietnamese fish. These results indicate that consumption of improperly prepared fish represents a significant risk of acquiring FZTs in this south Vietnam region.
Animals ; Aquaculture ; Fish Diseases/*epidemiology/parasitology ; Fishes ; Intestinal Diseases, Parasitic/epidemiology/parasitology/*veterinary ; Liver Diseases, Parasitic/epidemiology/parasitology/*veterinary ; Prevalence ; Trematoda/growth & development/*isolation & purification ; Trematode Infections/epidemiology/parasitology/*veterinary ; Vietnam/epidemiology ; Zoonoses/*epidemiology/parasitology

To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-alpha) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.
Animals ; Brain/parasitology ; Cytokines/*metabolism ; Female ; Gene Expression Regulation ; Heart/parasitology ; Interleukins/*metabolism ; Intestines/parasitology ; Larva Migrans, Visceral/*immunology/parasitology ; Liver/parasitology ; Lung/parasitology/pathology ; Mice ; Mice, Inbred C57BL ; Muscles/parasitology ; Spleen/parasitology ; Th1 Cells/immunology ; Th17 Cells/immunology ; Th2 Cells/immunology ; Toxascaris/*immunology

We trapped a rat (Rattus norvegicus) infected with Capillaria hepatica. At necropsy, grossly yellowish-white nodules (2-3 mm in diameter) were noted to be scattered on the liver's surface. Microscopically, granulomatous and fibrotic nodules that contained the eggs and/or adult worms of Capillaria hepatica were detected in the liver. Septal fibrosis was diffusely formed throughout the liver. There were a number of ED1-positive macrophages located in the sinusoids of the pseudolobules. On the double staining, myofibroblasts and mast cells were generally observed within the fibrous septa with the mast cells in close proximity to the myofibroblasts. We suggest that the interactions between macrophages, myofibroblasts and mast cells play a role in the septal fibrosis observed in rats infected by Capillaria hepatica.
Animals ; *Capillaria ; Enoplida Infections/immunology/parasitology/*veterinary ; Fibroblasts/immunology ; Liver/parasitology/pathology ; Macrophages/immunology ; Mast Cells/immunology ; Rats ; Rodent Diseases/*immunology/*parasitology/pathology