1.Research progress and prospect of electrical properties tomography for prostate.
Yang LIN ; Songshi DAI ; Shan'an ZHU
Journal of Biomedical Engineering 2012;29(5):987-990
We reviewed the research progress and prospect of electrical properties tomography for prostate in this paper. After the introduction of the basic principles of electrical impedance tomography (EIT) and magnetic resonance electric impedance tomography (MREIT), we presented the applications of the two techniques in electrical properties tomography of the prostate in detail. We then discussed the application prospects of induced current magnetic resonance electric impedance tomography (IC-MREIT) and magnetic resonance electrical properties tomography (MREPT) in the diagnoses of prostate cancer.
Electric Impedance
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Humans
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Magnetic Resonance Imaging
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methods
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Male
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Prostate
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physiology
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Prostatic Neoplasms
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diagnosis
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Tomography
2.Matrine suppresses the growth of human chronic myeloid leukemia K562 cells via inhibiting bcr-abl-mediated MEK-ERK pathway
Liuyang HE ; Haijun ZHOU ; Xiao SUN ; Zhichao ZHU ; Yu BAI ; Lijia JIANG ; Xuzhang LU ; Min ZHOU ; Sixuan QIAN ; Jianyong LI ; Lingdi MA
Cancer Research and Clinic 2015;(7):433-437,444
Objective To investigate the mechanism of matrine in inhibition of proliferation the proliferation of human chronic myeloid leukemia (CML) K562 cells via MEK-ERK signaling pathway. Methods Western blot was used to detect the expression of MEK1, ERK1/2, Shc and SHP2 (the signal effect molecules of MEK-ERK pathway) in K562 cells. The transcription and translation of bcr-abl and target protein (bcl-xL, Cyclin D1, c-myc and p27) were detected by RT-PCR and Western blot. Results Matrine was able to significantly inhibit the phosphorylation of MEK1, ERK1/2, Shc and SHP2 in K562 cells and suppress the protein and mRNA expression of bcr-abl. Moreover, the expressions of bcl-xL, Cyclin D1 and c-myc were down-regulated significantly, while the expression level of p27 (a negative regulator of cell cycle progression) was increased markedly after matrine treatment. Conclusions Suppression of the growth of human CML K562 cells is related to the inhibition of bcr-abl-mediated MEK-ERK pathway activity. The down-regulation of phosphorylated proteins or protein kinases activity in signaling pathways might be an important molecular mechanism in control the activity of MEK-ERK pathway.
3.Research progress of hepatectomy assisted by mixed reality technique for primary liver cancer
Liuyang ZHU ; Long YANG ; Jiancun HOU ; Yamin ZHANG
Chinese Journal of Hepatobiliary Surgery 2021;27(12):950-953
Mixed reality, as a new virtual simulation technology, has been initially applied in the field of surgery. In hepatectomy for primary liver cancer, mixed reality technology has its unique advantages in formulating and evaluating surgical plans before surgery, precise real-time navigation during surgery, and virtual liver teaching, making liver resections more accurate and personalized. This article summarizes the relevant literature at home and abroad in recent years, summarizes the research progress of mixed reality technology in assisted liver resection for primary liver cancer, and discusses its application potential and limitations.
4.Inhibitory effect of PARP inhibitor olaparib on acute myeloid leukemia HL-60 cells
Zhichao ZHU ; Yu BAI ; Xuzhang LU ; Xiao SUN ; Liuyang HE ; Chunjian QI
Practical Oncology Journal 2019;33(6):481-485
Objective The inhibitory effect of the PARP inhibitor olaparib on human acute myeloid leukemia HL-60 cells was studied. Methods The HL-60 cells in logarithmic growth phase were treated with different concentrations(1. 25,2. 5,5 and 10 μmol/L) of olaparib for different time. The CCK-8 assay was used to detect the inhibitory effect of olaparib on HL-60 cells. The apoptotic level of HL-60 cells was detected by Annexin-V/PI double staining method,and the expression of related signal proteins ( PARP-1 and caspase-3)in HL-60 cells was detected by Western blot. Results HL-60 cells were inhibited by olaparib at dif-ferent concentrations(1. 25,2. 5,5 and 10 μmol/L) for 48 h,and the inhibition rate gradually increased with the prolongation of the action time;at the same time,the apoptotic rate was increased in HL-60 cells after olaparib treatment for 48 h,showing a dose-de-pendent manner;the PARP activity was inhibited and caspase-3 was activated in HL-60 cells treated with olaparib. Conclusion The PARP inhibitor olaparib not only inhibits proliferation of HL-60 cells,but it also promotes apoptosis of HL-60 cells by inhibi-ting PARP activity and activating caspase-3.
5.Research progress of organoids in liver regenerative medicine
Liuyang ZHU ; Sen LIU ; Tao CUI ; Long YANG ; Chuanliang CHENG ; Pinsheng HAN ; Yamin ZHANG
Chinese Journal of Hepatobiliary Surgery 2024;30(1):72-76
Liver regenerative medicine can use functional liver cells to repair or replace damaged liver tissue and it is expected to be rapidly developed as an alternative treatment to liver transplantation. However, regenerative medicine requires cells with stable proliferation ability and liver cell characteristics. Liver organoids are derived from adult stem cells or pluripotent stem cells. They can be proliferated in large quantities and cultured for a long time in vitro, meanwhile maintain genetic stability, and simulate the structural and functional characteristics of organs in the body, providing a new strategy for liver regeneration. This article reviews liver organoids and their research progress in liver regenerative medicine, and discusses their application potential and existing limitations.
6.Physiological and enrichment characteristics of Paulownia fortunei seedlings under zinc, cadmium and their combined stress.
Xiuhong ZHU ; Hongmei CHENG ; Liuyang JI ; Guangxin RU ; Zhulin ZHAO ; Yaoqi CAI ; Daoyuan WEN
Chinese Journal of Biotechnology 2021;37(7):2463-2473
The hydroponic culture test method was used to study the physiological and biochemical responses of Paulownia fortunei seedlings under Zn stress, Cd stress, and combined Zn and Cd stress as well as changes in the enrichment and transfer characteristics of heavy metals. Under single and combined heavy metal stress, the biomass, plant height, and peroxidase (POD) activity of P. fortunei decreased as the treatment concentration increased. Combined Zn and Cd affected adversely plant height and biomass. As the concentration of Zn increased when applied alone, the chlorophyll content and catalase (CAT) activity of P. fortunei first increased and then decreased, the superoxide dismutase (SOD) activity increased, and the aboveground malondialdehyde (MDA) content first decreased and then increased. As the concentration of Cd increased when applied alone, chlorophyll content and CAT activity increased, and SOD activity and aboveground MDA content first increased and then decreased. Under both Cd and Zn, the physiological response was more complex. Cd in the seedlings of P. fortunei was concentrated in the root. In contrast, Zn was concentrated in the upper part of the ground, and its transfer coefficient was greater than 1.00. Thus, the addition of Zn promotes the transfer of heavy metals to the above-ground portions of plants. Generally, P. fortunei can effectively promote ecological restoration under complex forms of heavy metal pollution.
Cadmium
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Chlorophyll
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Metals, Heavy
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Plant Roots/chemistry*
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Seedlings
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Soil Pollutants
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Stress, Physiological
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Superoxide Dismutase
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Zinc