Objective To investigate the thrombolytic therapy in patients with cerebral infarction.Methods 208 patients with cerebral infarction were randomly selected as the research object,in which 96 patients receivd thrombolytic therapy,another 112 patients received conventional treatment.Results The scores of NIHSS thrombolytic group after treatment than in the conventional group decreased more obviously.Thrombolysis group before treatment and after treatment of the various stages of NIHSS score have significant difference (t =6.05,5.83,6.96,7.94,all P ＜ 0.01) ; thrombolytic therapy group and the conventional group after treatment of 90d after the Barthel index difference was statistically significant(t =4.67,P ＜ 0.05).Thrombolytic effect in treatment group is better than the conventional group,the difference was statistically significant (x2 =13.47,P ＜ 0.05).Conclusion Thrombolytic therapy can cerebral infarction affect of patients with influence,it can make the patient improved neurological function recovery,recover life skills,improve the quality of life.

Objective To study the incidence of coronary atherosclerosis in patients with stroke.Methods All patients were selected from January 2010 to January 2012,of which 63 stroke patients with first attack were enrolled as stroke group and other 63 patients with non-cerebral vascular disease were as control group.Coronary angiography was given 1-2 weeks since patients were admitted to hospital.Coronary artery stenosis ≥50％ was considered as coronary atherosclerosis.Gensini score was used to identify severity of coronary atherosclerosis.Results There were 16 patients (25.4％,16/63) identified as coronary atherosclerosis in stroke group and 4 patients (6.3％,4/63) in control group,and there was significant difference between two groups(x2=8.56,P ＜ 0.01).Gensini score was (3.37 ± 0.72) points in stroke group and (0.44±0.16) points in control group,and there was significant difference between two groups (P＜0.05).Conclusion Patients with first stroke have a higher incidence of coronary atherosclerosis.

As a kind of nervous system disease,migraine is more common in female,and has the clinical characteristics of repeated attacks,it is of great significance with standardized treatment in the control of the attacks.Yong female patients with migraine during pregnancy and lactation stage will face lots of special problems because they must first consider the impact of treatment on the mother and fetus.Generally,non-drug therapy is recommended as a first-line treatment,if it is not sufficiently effective,paracetamol is recommended during the pregnancy or sporadic use of sumatriptan,NSAIDs is not recommended during the first or third trimester of pregnancy.Preventive therapy should only be considered in the most severe cases.This review summarized recent documents of the safety of the most used antimigraine medications during pregnancy and breastfeeding,in order to provide treatment recommendations in clinical practice.

Objective To study and the succession of sarcosaphagous insects and their regular activity on carcass in Xinxiang area. Methods Six rats and five rabbits were sacrificed and placed at the remote grassland of Xinxiang Medical University in July 2015. The main insect species and the decomposition process were observed. The different decomposition stages and the community succession of insects were recorded. Results Seven main species could be identified belonging to 3 families from Diptera, main including Musca domestica, Chrysomya megacephala, Lucilia sericata, Boettcherisca peregrina, Helicophagella melanura. Muscina stabulans and Fannia prisca. And showed regulations of the succession of sarcosaphagous flies community. Conclusion It may be used for estimating postmortem interval in Xinxiang area.

Objective To investigate the role of p38 mitogen-activated protein kinase(p38MAPK) pathway in the proliferation of SHR vascular smooth muscle cells(VSMC) induced by angiotensin Ⅱ(Ang Ⅱ) and platelet-derived growth factor-BB(PDGF-BB).Methods VSMC were obtained from SHR thoracic aorta.DNA synthesis was quantified by measuring [3H]-thymidine incorporation.The p38MAPK activity was evaluated by immunobloting technique with phospho-p38MAPK antibody.Results 1) Ang Ⅱ(10-8-10-6 mol/L),PDGF(3-30 ng/L) dose-dependently increased the proliferation activity of VSMC([3H]-TdR incorporation).Ang Ⅱ(10-7 mol/L) or PDGF(10 ng/L) significantly increased [3H]-TdR incorporation rate in VSMCs(Ang Ⅱ:11 588?1322 vs control 2546?207 counts/min,P

The transformation from the biomedical mode to socio-psycho-biological mode requires higher demands for medical education and comprehensive quality for the medical talents.The existing method of neurology education has disadvantages and cannot meet the needs of modern social life for doctors.By analyzing the neurology education situation that can not be adapted to progression of society,some measures to enhance neurology education are put forward.

Objective:To observe the changes of salivary Lysozyme (LZM),immunoglobulin A (IgA),lactate dehydrogenase (LDH),mean grey values (MGVs)of hard tissue of teeth and caries index(CSI)in patients after oral administration of glucocorticoid (GC).Methods:30 patients with oral administration of GC were included.The mixed saliva samples before and after 6 and 12 months of GC treatment were collected.LZMand IgA and LDH were examined.Surface layer panaorama films were taken and MGVs of hard tissue of teeth were measured.The decayed,missing and filled tooth(DMFT),decayed,missing and filled surface (DMFS), CSI were recorded.The same indexes were obtained from 30 age and gender matched healthy subjects as the control data.All statisti-cal analyses were performed using SPSS 17.0 software package.Results:After taking GC orally,the concentration of LZM,IgA and MGVs of hard tissue of teeth in the patients were lower than those in the controls(P <0.05),the LDH concentration,DMFT,DMFS and CSI in the patients were increased(P <0.05).In the patients the MGVs of hard tissue of teeth was negatively correlated with DM-FT,DMFS and CSI(P <0.01),LDH concentration was positively correlated with CSI(P <0.01),the concentration of LZMand IgA were negatively correlated with CSI(P <0.05).No change of the indexes was found in the controls.Conclusion:Oral administration of GC may increase DMFT,DMFS and CSI,decrease MGVs of hard tissue of teeth significantly reduce,the patients may be under higher risk of caries.

Objective:To investigate the predictive value of the clinicopathological features of breast cancer for pathological complete response (pCR) after neoadjuvant chemotherapy (NAC) and to establish a predictive model based on the clinicopathological features.Methods:Clinicopathological data collected from 182 patients who underwent NAC and surgical treatment in Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine from Jan. 2013 to Dec. 2019 were retrospectively analyzed. The univariate and multivariate analysis were used to analyze the relationship between clinicopathological features and pCR after neoadjuvant chemotherapy. The predictive value in predicting the efficacy of NAC was evaluated, the receiver operating characteristic (ROC) curve and Nomogram prediction model were constructed.Results:Multivariate Logistic regression analysis showed that progesterone receptor (PR) , human epidermal growth factor 2 (HER2) and platelet distribution width (PDW) were independent predictors of pCR after NAC for breast cancer. The area under the curve (AUC) of model for predicting efficacy of NAC was 0.810 (95% CI:0.745-0.864) and the sensitivity and specificity was 68.75% and 82.67% respectively when the Jordan Index is at its maximum. Conclusion:ER-, HER2+ and PDW≤13.4% show better efficacy of NAC. The Nomogram model based on them can accurately predict the efficacy of NAC and can provide a reference for the selection of treatment options in clinical diagnosis and treatment.

Objective To evaluate the clinical value of SAA by detecting their expression levels in patients with lung cancer and the analysis of the relativity of SAA for early diagnosis.Methods There are 243 cases specimens obtained from lung cancer patients who were newly diagnosed and without any treatment in Zhejiang Cancer Hospital from April 2014 to June 2015.The 243 lung cancer individuals were 147 male, and 96 female, their ages ranged from 29 to 85 years, with an average age of 63 years.The distribution of pathological type was as follows:95 patients were adenocarcinoma, 102 patients were squamous carcinoma, and 46 patients were small cell carcinoma.The distribution of TNM staging systems was as follows: 59 patients in stage 1and stage 2, 54 patients in stage 3, and 130 patients in stage 4.While 179 cases physical examination as the control.There were 94 individuals male, and 85 individuals female.Their ages ranged from 26 to 86 years, with an average age of 61 years.By using latex enhanced immune turbidimetric method, serum SAA concentrations in patients with lung cancer and healthy controls were checked on the Hitachi-7600 automated chemistry analyzer ( Hitachi ).The comparisons of all analyses values between healthy controls and lung cancer were estimated by two independent samples nonparametric tests ( Mann-Whitney U).The association between SAA and lung cancer prognostic factors such as age, smoking status and metastasis, was evaluated by spearman correlation and multivariate analysis.Results The median and interquartile spacing of SAA concentration was 42.36 mg/L (9.35, 74.22) in lung cancer patients.While 24 mg/L ( 3.25, 21.45 ).The median level of SAA in lung cancer patients (42.36 mg/L) were significantly higher than in healthy controls (11.24 mg/L), and difference reached statistically significant (Z=-2.403,P=0.006).Nevertheless, there was no significant difference in SAA concentrations among the different pathological types(Z=-1.013, P=0.339), ages (Z=0.578, P=0.458) and gender(Z=0.726, P=0.246) of lung cancer patients.While the level of SAA in has smoking status (Z=-2.282, P=0.013) and distant metastasis (Z=-2.138, P=0.017) of lung cancer was higher.By drawing ROC curve, the cut off value of SAA in distinguishing lung cancer with healthy control was 14.48 mg/L.Meanwhile, the AUC was 0.811, the accuracy is 89.12% and the sensitivity was 88.73%.Serum concentration was positively related with smoking status ( r =0.331, P =0.018 ) and distant metastasis ( r =0.372, P=0.015 ) by Spearman correlation analysis .Conclusion Serum SAA concentrations may contribute to the auxiliary diagnosis of lung cancer, evaluate the clinical stage and distant metastases of lung cancer.

Objective To construct the prokaryotic expression vector pET30a( +)-VH-mms13 and identification of its protein after induced with IPTG.Method Heavy chain variable region VH gene of typeⅣcollagenase monoclonal antibody and magnetosome membrane protein gene mms13 were amplified separately,the fusion gene VH-linker-mms13 were synthesized by SOE-PCR technique and inserted into pET30a ( +) plasmid, which was confirmed by restriction enzyme digest and sequencing.Then the recombinant plasmid pET30a ( +)-VH-mms13 was transform into E.coli DE3 and induced with 0.4 mmol/L IPTG.The fused protein was identified by SDS-PAGE and Western blot.Results The length of fusion gene VH-mms13 was 738 bp,and the sequence was correct.After induced with IPTG,the fused protein was found in the inclusion body and Western blot results suggested that the fused protein can bind with His-tag antibody specifically.Conclusion Expression vector pET30a ( +)-VH-mms13 is successfully constructed and the fusion protein has good immunogenicity,which lay the foundation for the development of biomagnetism-targeted drug.