Objective:To establish a reliable sequence-based typing method for KIR2DS4 and study its allele polymorphism in Chinese Han population.Methods:Using PCR - SSP method to detect the positive or negative of KIR2DS4 gene in 222 random Chinese Han individuals, and then using the method of high fidelity and long-fragment PCR - SBT to amplified, sequence and genotype the exons 4 and 5 of KIR2DS4 positive individuals.Results:We successfully amplify the fragment with 3.2 kb length contains exons 4 and 5 of KIR2DS4 and detected the KIR2DS4 allele frequency in Chinese Han population. 209 KIR2DS4 positive individuals were detected, and the positive rate is 94.1%. By sequence-based typing, we identified 12 genotypes and 7 alleles of KIR2DS4. The 6 known alleles and their detection frequency is as follows: KIR2DS4 * 00101/011 (180, 81.1%), KIR2DS4 * 010 (53, 23.9%), KIR2DS4 * 004 (34, 15.3%), KIR2DS4 * 003 (15 and 6.8%), KIR2DS4 * 006 (2, 0.9%) and KIR2DS4 * 015 (1, 0.5%). In this study, we found a new allele, KIR2DS4 * 016, with the difference in exon 5 comparing its most similar allele KIR2DS4 * 010. In the exon 5 of KIR2DS4 * 010, there is a 22bp-deletion, while the exon 5 of KIR2DS4 * 016 is normal. This is not a rare allele because it was detected 3 times in studied population and with the frequency of 1.4%. The sequence of the new allele sequence has been submitted to GenBank (accession no.: KC414890) and the IPD - KIR database (submission no.: IWS40001804), and was nominated by WHO nomenclature committee for HLA system. Conclusion:In this study, a sequence-based typing method for KIR2DS4 was established, and the polymorphism data of KIR2DS4 in Chinese Han population was enriched by studying the allele polymorphism and new allele.

Objective To compare the performance of Montreal cognitive assessment scale (MoCA) and minimental state examination scale (MMSE) in screening HIV-associated neurocognitive disorders (HAND).Methods A case-control study of 127 HIV + and 60 HIV-individuals.All the subjects' cognitive functions were assessed using MoCA and MMSE separately.Results Mean MoCA score in cases was 26.28 ± 2.43 compared to 27.33 ± 1.30 in controls (P ＜ 0.01).Mean MMSE score in cases was 27.45 ± 1.31 compared to 27.70 ±0.89 in controls (P =0.139).Using the MoCA screening revealed 43 (33.9％) cases had HAND compared with 6 (6.7 ％) controls (x2 =16.01,P ＜ 0.001).Using MMSE,6 (4.7％) cases and 1(1.7％) control had HAND (P=0.124).Conclusion The MoCA scale is more sensitive in deteting HAND than MMSE and is suited for the early cognitive screening of HIV patients in clinic.

Objective:To determine the effects of preperitoneal balloon (PPB) tamponade with different volumes of fluid on hemodynamically unstable pelvic fracture-associated arterial and venous hemorrhage in a swine model.Methods:A model of open-book pelvic fracture with injuries to external iliac vessels was established in 18 female 12-month old Bama miniature pigs. After the successful establishment of hemodynamically unstable pelvic fracture with vascular injury was confirmed by contrast agent imaging, the animals were randomized into 3 even groups ( n=6): a control group (group C) subjected to PPB tamponade with 0 mL fluid injected, group T1 subjected to PPB tamponade with 500-mL fluid injected, and group T2 subjected to PPB tamponade with 1,000-mL fluid injected. The 3 groups were compared in terms of 60-min survival rate, balloon pressure, peritoneal pressure, bladder pressure, 70-min survival rate, blood loss, and infusion volume. Results:There was no statistically significant difference in the basic hemodynamic or other experimental indicators among the 3 groups before experiment, indicating comparability ( P>0.05). The 60-min survival rate in group T2 was 100.0% (6/6), significantly higher than those in group C and group T1 [0.0% (0/6), 0.0% (0/6)] ( P<0.05). After fluid injection, the balloon pressure and preperitoneal pressure in group T2 were respectively (127.2±4.7) mmHg and (34.5±3.6) mmHg, significantly higher than those in group T1 [(78.7±3.8) mmHg and (13.7±2.8) mmHg] and in group C [0 mmHg and (9.0±1.4) mmHg], and the 2 indicators in group T1 were significantly higher than those in group C (all P<0.05). After fluid injection, there was no statistically significant difference among groups C, T1, and T2 in bladder pressure [(6.7±1.0) mmHg, (5.8±1.9) mmHg, and (6.0±1.1) mmHg] or in bleeding volume [(1,163.0±191.3) mL, (1,212.0±148.4) mL, and (975.0±133.2) mL] (all P≥ 0.05). The infusion volume in group T1 [(1,250.0±225.8) mL] was significantly larger than that in group C [(951.7±177.8) mL] ( P<0.05). No colorectal or bladder injuries were found by the anatomy of the experimental animals in 3 groups. Conclusions:PPB tamponade with 1,000-mL fluid injected in a swine model can efficiently control pelvic fracture-associated arterial and venous hemorrhage, and increase the 60-min survival rate with no colorectal or bladder injuries.

Objective:To explore the effect of eCASH nursing model on the prevention of delirium and quality of care for patients with coronary heart disease in ICU after percutaneous coronary intervention (PCI) .Methods:A total of 188 patients with coronary heart disease who underwent PCI in Henan Provincial People's Hospital from January 2019 to December 2020 were selected by the convenient sampling method and they were admitted to ICU after surgery. Patients admitted from January to December 2019 were set as the control group ( n=94) and received routine care. Patients admitted from January to December 2020 were set as the observation group ( n=94) and received eCASH nursing mode. The incidence of delirium [Confusion Assessment Method for the Intensive Care Unit (CAM-ICU) ], hospitalization (bed time, ICU stay time, total length of stay) , nursing quality (Inpatient Nursing Job Satisfaction Scale) and complications during hospitalization were compared between the two groups. Results:The incidence of delirium, bed time, length of stay in ICU and total length of stay in observation group were lower than those in control group and the score of Inpatient Nursing Job Satisfaction Scale was lower than that in control group, and the differences were statistically significant ( P<0.05) . During hospitalization, there was no statistically significant difference in the incidence of puncture site hematoma, skin blister, infection and limb venous thrombosis between 2 groups ( P>0.05) . The incidence of urinary retention in observation group was lower than that in control group, and the difference was statistically significant ( P<0.05) . Conclusions:The eCASH nursing mode can help ICU patients with coronary heart disease to prevent delirium after PCI, shorten the length of hospital stay, improve quality of nursing and help to reduce complications during the hospital stay.