Objective:To explore the clinical significance detecting adrenomedullin (ADM)and high sensitive C reac-tive protein (hsCRP)in patients with acute coronary syndrome (ACS).Methods:A total of 51 inpatients,which were eliminated for coronary heart disease (CHD)by coronary angiography (CAG),were enrolled as non CHD group,and 124 ACS patients diagnosed by CAG were regarded as ACS group.ADM and hsCRP concentrations were measured before and after CAG in non CHD group and before and after percutaneous coronary intervention (PCI) in ACS group,and they were compared between two groups.Results:Before CAG/PCI,compared with non CHD group,ADM [(28.7±1.6)pg/ml vs.(36.7±2.5)pg/ml]and hsCRP [(4.74±3.21)mg/L vs.(27.65±11.76) mg/L]levels significantly rose in ACS group,P<0.05 or <0.01.Compared with before PCI,there were signifi-cant increase in levels of ADM [(36.7±2.5)pg/ml vs.(47.1±5.3)pg/ml]and hsCRP [(27.65±11.76)mg/L vs.(39.53±4.83)mg/L]in ACS group after PCI,P<0.01 both,and significantly higher than those of non CHD group (P<0.05~<0.01).There were no significant difference in ADM and hsCRP levels in non CHD group be-tween before and after CAG (P>0.05 all).Conclusion:Detecting arenomedullin combined high sensitive C-reac-tive protein is gelpful to early diagnosis and evaluating lesion severity for acute coronary syndrome.

ObjectiveTo observe the clinical effect of low molecular weight heparin calcium in the treatment of chronic obstructive pulmonary disease combined with pulmonary heart disease.MethodsA total of 64 patients with chronic obstructive pulmonary disease combined with pulmonary heart disease from December 2008 to December 2010 were enrolled in this study and divided into treatment group and control group by random digits table with 32 cases each.The control group was given routine treatment and the treatment group was given low molecular weight heparin calcium for 7 days at the basis of routine treatment.ResultsThe total effective rate in treatment group[ 90.6％ (29/32) ] was significantly higher than that in control group [ 68.8％ (22/32) ] (P ＜ 0.05 ).In treatment group,the levels of arterial carbon dioxide tension (PaCO2) and arterial oxygen tension (PaO2) after treatment [(54.64±9.63),(74.21 ± 11.76) mm Hg (1 mm Hg =0.133 kPa)] were significantly decreased compared with before treatment [(78.66 ± 11.22),(53.42 ± 8.84 ) mm Hg ] (P ＜ 0.01 ).In control group,the levels of PaCO2 and PaO2 after treatment [ (61.10 ±7.24),(65.07 ± 8.21 ) mm Hg] were significantly decreased compared with before treatment[ (79.52 ± 12.54),(51.35 ± 7.31 ) mm Hg ] (P ＜ 0.05 ).After treatment the levels of PaCO2 and PaO2 in treatment group were better than those in control group (P ＜ 0.05).ConclusionsThe low molecular weight heparin calcium in treatment of chronic obstructive pulmonary disease combined with pulmonary heart disease can effectively improve the clinical manifestation.It is worth the clinical promoted application.

H type hypertension is essential hypertension complicated elevated plasma homocysteine level ,which ac-counts for about 75% in Chinese adults with hypertension and is closely related to cerebral stroke and other cardio-vascular diseases .Lowering homocysteine level in patients with hypertension possesses important significance for preventing stroke .

Objective To investigate the distribution of traditional Chinese medical syndromes and clinical characteristics of influenza in South of Five Ridges.Methods A retrospective analysis was carried out in 162 cases of influenza patients admitted from outpatient department,emergency department and inpatient department of the First Affiliated Hospital of Guangzhou University of Chinese Medicine from 2014 to 2016.The distribution of clinical manifestations and syndrome types of the included influenza patients was analyzed.Results The average age of the included influenza patients was 35.76 ± 11.4 years old.The clinical syndromes were mainly characterized by fever,aversion to cold and chills,fatigue and weakness.And damp-accumulation manifestations of heaviness in the body,poor appetite,dry mouth without willing to drink,nausea and vomiting were also predominant.The main syndrome types were wind-heat attacking defense phase syndrome,wind-cold fettering exterior syndrome,heat-toxin attacking lung syndrome,heat-toxin accumulating lung syndrome,and damp syndrome.Of the syndrome types,wind-heat attacking defense phase syndrome and heat-toxin attacking lung syndrome were the leading types,accounting for 77.79％ and interweaving with damp syndrome and heat-damp syndrome.Conclusion The syndromes of influenza patients in South of Five Ridges are usually complicated by damp syndrome or damp-heat syndrome,and the predominant syndrome type is wind-heat interweaved with damp syndrome,which is correlated with the climate being damp and hot in South of Five Ridges.

This study was intended to evaluate the effects of hypoxic exposure on gene expression and coordination of cytochrome oxidase (COX) subunits I (COX I) and IV (COX IV) encoded by mtDNA and nDNA respectively in rat cerebral cortex. Male Wistar rats were exposed to hypoxia in a hypobaric chamber simulating high altitude at 5000 m for 2, 5, 15 and 30 d. Control rats were fed outside the hypobaric chamber (the height was 300 m above sea level). Rats were sacrificed and mitochondria from cerebral cortex were isolated by differential centrifugation at each time point. COX I and COX IV proteins in isolated rat cerebral cortex mitochondria were detected by Western blot analysis and mRNA in the cerebral cortex by RT-PCR. The ratios of protein and mRNA were used to estimate the coordinative expression of two subunits. The results showed that COX I mRNA increased significantly at 2 and 5 d, and decreased to the control level at 15 and 30 d; COX IV mRNA remarkably increased at 2, 5 and 15 d, and dropped below the control level at 30 d. The mRNA ratio of COX IV to COX I reached a peak at 15 d, but showed no differences between other time points. The Western blot analysis of COX I and COX IV in isolated rat cerebral cortex mitochondria showed no obvious changes during hypoxic exposure. Our findings demonstrate that hypoxia can affect mRNA expression of COX I and COX IV and their coordination, while protein expression of both subunits are stable and coordinative. This study suggests that the expression of COX I and COX IV proteins during hypoxic exposure is coordinately regulated by post-transcriptional mechanisms.
Animals ; Cerebral Cortex ; metabolism ; Electron Transport Complex IV ; metabolism ; Gene Expression Regulation, Enzymologic ; Hypoxia ; metabolism ; Male ; Mitochondria ; metabolism ; Rats ; Rats, Wistar

OBJECTIVETo explore the relationship between nurse occupational stress and salivary alpha- amylase (SAA).

METHODSEvaluation of occupational stress was conducted in 131 nurses. The activity of SAA was determined using enzyme-linked immunosorbent assay (ELISA).

RESULTSThe activity of SAA in nurses varied with age and working years. The baseline, work period, recovery, average activities of >35 age group were less than those of ≤ 30 age group; work period, recovery, average activities of ≤ 10 years group were higher than other two groups; there was no statistical difference between SAA vitalities of different degree groups (P>0.05). In nurses with high scores for job demands, the activity of SAA in working period was significantly higher than that in nurses with low scores (P < 0.05). The baseline SAA activity in nurses with high scores for role conflict and ambiguity was significantly higherthan thatin nurses with low scores (P < 0.05). The baseline SAAactivity was positively correlated with workload, role conflict, and role ambiguity (P < 0.05). The activity of SAA in working period was negatively correlated with task control, decision control, and technology utilization (P < 0.05), and was positively correlated with quantitative load, load change, work monotony, and workload (P < 0.05). The activity of SAA in recovery period was negatively correlated with task control, decision control, resource control, and technology utilization (P < 0.01). The average activity of SAA was negatively correlated with task control, decision control, resource control, technology utilization, opportunity for participating in decision-making, and promotion (P < 0.05), and was positively correlated with quantitative load, load change, workload, and role ambiguity (P < 0.05).

CONCLUSIONThe occupational stress in 131 nurses is correlated with the activity of SAA, which can be used as an objective biomarker for identification and evaluation of occupational stress.

Biomarkers ; Humans ; Nurses ; psychology ; Occupational Diseases ; epidemiology ; Salivary alpha-Amylases ; analysis ; Stress, Psychological ; diagnosis ; epidemiology ; Work ; Workload

OBJECTIVETo investigate the correlation between nurse job burnout and salivary lysozyme activity.

METHODSThe saliva samples of 131 subjects were collected at four time points for two consecutive days with saliva collection tubes. The acquisition time points were 8:00 (baseline concentration), 10:00 (morning), 15:30 (afternoon), and 17:30 (recovery period). At the same time every subjects completed the job burnout questionnaire to investigate their general demographic characteristics and job burnout level. The salivary lysozyme concentration was measured with ELISA. The data were analyzed by partial correlation analysis and multiple stepwise regression analysis.

RESULTSThere were significant differences in the salivary lysozyme activity between subjects with different ages, working years, and education levels. The work period vitality and the average energy of ≤ 30 age group were higher than other two groups and the recovery energy was higher than >35 age group. Working period vitality, the average energy of group >15 years were less than ≤ 10 years group. The work period energy and the average energy of university (college) and above group were lower than high school (secondary) and the following group. Job burnout and its three dimensions had a significant negative correlation with salivary lysozyme concentration (P < 0.01). Depersonalization and emotional exhaustion were the negative impact factors for salivary lysozyme activity at baseline. Emotional exhaustion and personal fulfillment were the negative impact factors for salivary lysozyme activity during the working period. Personal fulfillment was the negative factor for salivary lysozyme activity during the recovery period and the average salivary lysozyme activity.

CONCLUSIONSalivary lysozyme activity is sensitive for nurse job burnout, so it can be used as an objective evaluation index of job burnout.

Burnout, Professional ; epidemiology ; psychology ; Emotions ; Fatigue ; Humans ; Muramidase ; analysis ; Nurses ; psychology ; Occupational Diseases ; epidemiology ; psychology ; Regression Analysis ; Salivary Proteins and Peptides ; analysis ; Surveys and Questionnaires

Objective: To explore the relationship between occupational stress and physiological and biochemical indexes, to research the health effect of the occupational stress in aircrew. Methods: 450 aircrews were conducted with the OSI-R questionnaire survey, examine the level of blood pressure, blood routine, ALT and UA. Results: The concentration of HB was positively related with task conflict and entertainment and leisure (β=0.262 and 0.106, both P<0.05) , while heavy task, task discomfort and psychological stress reactions were negatively related with HB (β=-0.163, -0.102, and -0.137, all P<0.05) ; task conflict and self-care were positively related with RBC (β=0.221 and 0.159, both P<0.01) , heavy task, psychological stress reactions and social support were negatively related with RBC (β=-0.157, -0.119, and -0.113, all P<0.05) ; task ambiguity and self-care had a positive relationship with ALT (β=0.144 and 0.159, both P<0.01) while heavy task, psychological stress reactions and social support had a negative relationship with ALT (β=-0.176, -0.096, and -0.102, all P<0.05) ; self-care was positively related with SBP (β=0.170, P<0.01) , task discomfort, interpersonal stress reactions and social support were negatively related with SBP (β=-0.093, -0.103, and -0.111, all P<0.05) ; while self-care was positively related with DBP (β=0.139, P<0.01) , social support was negatively related with DBP (β=-0.114, P<0.05) . Conclusion Occupational stress of the aircrew is significantly related with blood pressure, RBC, ALT and UA, occupational stress can make effects on the health of aircrew.

Objective To investigate the effects of dulaglutide combined with insulin glargine on glucose and lipid metabolism,pancreatic islet function,oxidative stress and bone metabolism indica-tors in patients with type 2 diabetes mellitus.Methods A total of 98 patients with type 2 diabetes mellitus were randomly divided into insulin glargine group and combined group,with 49 cases in each group.The insulin glargine group was treated with insulin glargine and metformin,while the com-bined group was treated with dulaglutide,insulin glargine and metformin.The levels of blood glu-cose,blood lipids and pancreatic islet function were compared between the two groups before and after treatment;enzyme immunoassay analyzer was used to detect the levels of oxidative stress indicators;immunoturbidimetry was used to detect bone metabolism indicators;the urinary microalbumin to creat-inine ratio(UACR)was calculated in both groups,and the therapeutic effect was compared between the two groups.Results After treatment,the levels of fasting plasma glucose(FPG),2-hour post-prandial glucose(2 hPG)and glycated hemoglobin(HbA1c)in the combined group were significantly lower than those in the insulin glargine group(P＜0.05).After treatment,the levels of total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)in the combined group were significantly lower than those in the insulin glargine group,while the level of high-density lipo-protein(HDL)was significantly higher than that in the insulin glargine group(P＜0.05).After treatment,the homeostatic model assessment of insulin secretion(HOMA-IS)in the combined group was significantly higher than that in the insulin glargine group,while the homeostatic model assess-ment of insulin resistance(HOMA-IR)was significantly lower than that in the insulin glargine group(P＜0.05).After treatment,the levels of catalase(CAT)and osteocalcin(OC)in the combined group were significantly higher than those in the insulin glargine group,while the levels of lipid per-oxide(LPO)and β-collagen degradation products(β-CTX)were significantly lower than those in the insulin glargine group(P＜0.05).After treatment,the UACR in the combined group was(33.26±3.37)mg/g,which was significantly higher than(25.49±2.83)mg/g in the insulin glargine group(P＜0.05).The total effective rate in the combined group was 93.88％,which was significantly higher than 79.59％in the insulin glargine group(P＜0.05).Conclusion Dulaglu-tide combined with insulin glargine can effectively regulate the blood glucose and lipid levels of pa-tients with type 2 diabetes,improve the function of pancreatic islets,alleviate the oxidative stress re-action,improve bone metabolism,and regulate the UACR level.

Objective To investigate the effects of dulaglutide combined with insulin glargine on glucose and lipid metabolism,pancreatic islet function,oxidative stress and bone metabolism indica-tors in patients with type 2 diabetes mellitus.Methods A total of 98 patients with type 2 diabetes mellitus were randomly divided into insulin glargine group and combined group,with 49 cases in each group.The insulin glargine group was treated with insulin glargine and metformin,while the com-bined group was treated with dulaglutide,insulin glargine and metformin.The levels of blood glu-cose,blood lipids and pancreatic islet function were compared between the two groups before and after treatment;enzyme immunoassay analyzer was used to detect the levels of oxidative stress indicators;immunoturbidimetry was used to detect bone metabolism indicators;the urinary microalbumin to creat-inine ratio(UACR)was calculated in both groups,and the therapeutic effect was compared between the two groups.Results After treatment,the levels of fasting plasma glucose(FPG),2-hour post-prandial glucose(2 hPG)and glycated hemoglobin(HbA1c)in the combined group were significantly lower than those in the insulin glargine group(P＜0.05).After treatment,the levels of total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)in the combined group were significantly lower than those in the insulin glargine group,while the level of high-density lipo-protein(HDL)was significantly higher than that in the insulin glargine group(P＜0.05).After treatment,the homeostatic model assessment of insulin secretion(HOMA-IS)in the combined group was significantly higher than that in the insulin glargine group,while the homeostatic model assess-ment of insulin resistance(HOMA-IR)was significantly lower than that in the insulin glargine group(P＜0.05).After treatment,the levels of catalase(CAT)and osteocalcin(OC)in the combined group were significantly higher than those in the insulin glargine group,while the levels of lipid per-oxide(LPO)and β-collagen degradation products(β-CTX)were significantly lower than those in the insulin glargine group(P＜0.05).After treatment,the UACR in the combined group was(33.26±3.37)mg/g,which was significantly higher than(25.49±2.83)mg/g in the insulin glargine group(P＜0.05).The total effective rate in the combined group was 93.88％,which was significantly higher than 79.59％in the insulin glargine group(P＜0.05).Conclusion Dulaglu-tide combined with insulin glargine can effectively regulate the blood glucose and lipid levels of pa-tients with type 2 diabetes,improve the function of pancreatic islets,alleviate the oxidative stress re-action,improve bone metabolism,and regulate the UACR level.