The case teaching method was applied in the course of Clinical Rehabilitation. The key factors included the case design, operation and assessment in its working program. In the teaching of Clinical Rehabilitation, the use of the case teaching method can stimulate the interest of studence and enhance the effect of teaching.

Objective To observe the effect of rhythmic auditory stimulation (RAS) on gait of stroke hemiplegic patients. Methods From April, 2014 to April, 2015, eleven stroke hemiplegic patients were divided into RAS group (n=6) and control group (n=5) randomly. Both groups accepted routine rehabilitation, and the RAS group accepted RAS walking exercise, while the control group accepted verbal in-structed walking exercise. They were tested with Brunnstrom's Stage and gait analysis before and three weeks after training. Results There was no significant increase in Brunnstrom's Stage (Z<1.414, P>0.05) in both groups after training. The RAS group increased in stride length, velocity and cadence (t>4.829, P<0.01) after training, and improved in temporal symmetry (t=3.323, P<0.05), while there was no significant change in all of the indices in the control group (t<1.765, P>0.05). Conclusion Walking exercise under RAS facilitates to improve gait in stroke hemiplegic patients.

BACKGROUND: Lanceolata and astragalus can improve myocardial ischemia, which are widely used in clinics.OBJECTIVE: To compare the effects of extract of lanceolata and astragalus on electrocardiogram (ECG) of rats with experimental myocardial ischernia.DESIGN: Completely randomized and controlled study.SETTING: Pharmacological College of Harbin Commerce University.MATERIALS: The experiment was carried out at the Laboratory of Pharmacological College of Harbin Commerce University from September to December 2002. Totally 30 healthy adult Wistar rats were divided randomly into 3 groups: control group, lanceolata group and astragalus group with 10 in each group.METHODS: ① After anesthesia, lead ECG Ⅱ of rats was recorded.30 g/kg saline, 30 g/kg lanceolata extract and 30 g/kg astragalus extract [extract of lanceolata and astragalus (raw drug: 1 g/mL)] were injected into rats in the control group, lanceolata group and astragalus group respectively. After 1-3 minutes, 0.5 U/kg pituitrin was injected intravenously (for model of myocardial ischemia), and then saline was used to dilute from 0.1to 0.2 mL for 10-second injection. Changes of ECG after injection of pituitrin were recorded with LMA-2B Ⅱ physiological recorder (migration of T wave and ST section and change of heart rate). ② Differences of data were compared with t test.MAIN OUTCOME MEASURES: Height of T wave and Migrations of ST section and changes of heart rate of ECG of rats before and after medication.RESULTS: Data of totally 30 rats was entered the final analysis. ①Height of T wave: The height was higher after medication than that before medication in three groups (P ＜ 0.01), but that in astragalus group was obviously lower than that in lanceolata group [(1.63±3.81), (2.18±2.93) mm,P ＜ 0.01]. ② Heart rate: The rate was lower after medication than that of before medication in three groups (P ＜ 0.01), and that in astragalus group was obviously lower than that in lanceolata group [(212.21 ±5.01),(221.18±5.66) time/minute, P ＜ 0.01]. ③ Height of ST section: There were not significantly different in three groups (P ＞ 0.05), and the difference was not obvious before and after medication (P ＞ 0.05).CONCLUSION: ① Both astragalus and lanceolata can protect experimental myocardial ischemia induced by pituitrin. ② Effect of lanceolata on reduction of T-wave height is stronger than that of astragalus, but the effect of lanceolata on reduction of heart rate is weaker than that of astragalus.

This paper Summarized Wu Jutong's academic thought and diagnosis and treatment characteristics. The contribution of Wu Jutong to the traditional Chinese medicine was his legislation and theory of innovation, especially for the treatment of febrile diseases. Many prescriptions and theories that he left has been further improved TCM in exogenous diseases and febrile diseases treatment. Besides he also made great achievements on the diagnosis and treatment of arthralgia syndrome and diarrhea diseases. The academic thought and clinical characteristics of Wu Jutong had important practical significance to the prevention and control of emerging infectious diseases and infectious diseases.

Objective To observe the distribution of ER? in the sigmoid colon specimens from the patients with slow transit constipation(STC).Methods The expression of ER? was detected by immunohistochemical staining in formalin-fixed,paraffin-embedded sigmoid colon tissues from 16 cases of STC,and from 8 cases of rectal cancer at Dukes' stage A,2 cases of rectal villous adenoma(10 as control).The positive cells were analyzed by computer assistant image analysis system.Results ER? immunoreactivity was present in normal epithelium and interstitial cells of the sigmoid colon tissues in the two groups,but nucleus-positive in STC group and cytoplasm-positive in control group.In comparison with the control group,the ER? immunoreactivity in STC group was much lower(P

Objective　To investigate the involvement of cell cycle negative regulator p16 in the replicative senescence of normal human diploid fibroblasts. 　Methods　 p16 cDNA and retroviral vector was introduced into normal human fibroblast 2BS cells by transfection technique. Then the effects of p16 on replicative cellular senescence of 2BS cells were examined. 　Results　 Compared with the control cells, 2BS cells transfected with p16 cDNA showed significant suppression of growth rate (decrease 50%) with cell cycle arrested at G1 phase. This phenomenon similar to that of the senescent cells, with the cellular response to stimulation by growth factor decreased 79.4%, showing the characteristics in morphology of senescent fibroblasts. 　Conclusions　 The over expression of p16 gene contributes to the process of cellular senescence of normal human diploid fibroblasts.

The neuromechanism of language function recovery in patients with aphasia after stroke is still unclear. It is supported that the language function of aphasia after extensive damage of left hemisphere mainly recovered through activating the right hemisphere of ho-mologous regions to improve the function of speech. Melodic intonation therapy (MIT) can be achieved by melody tone and left hand clap to activate the right hemisphere of the frontal temporal lobe, which is particularly applicable to improve language function of non-fluent aphasia patients with large left hemisphere lesion. Non-invasive brain stimulation techniques (NBS) changes brain activity through stimulat-ing affected or contralateral side, which has been used to facilitate language functional recovery after stroke. Excitatory transcranial stimula-tion on the right hemisphere homology combined with melodic intonation therapy can promote language recovery of patients with extensive damage of left hemisphere after stroke. It is valuable to combine traditional speech training with MIT and NBS to activate related language functional areas to maximum improvement of language recovery after stroke.

Objective: To obtain a strain which has stronger antagonistic effect against Streptococcus mutans(S. mutans), and then to study the antagonistic effect of the fermentation filtrate of the strains. Method:Surface soil samples were harvested and used in an in vitro bacteria culture system for screening of antagonistic strains against Streptococcus mutans. Ferment filtrate of the strains was respectively prepared and the antagonism of the filtrate against S. mutans was examined.Result:A strain with strong restrain effect on Streptococcus mutans was found and was identified to be Bacillus licheniformis. The strain was named strain 1#. At pH 7.0, 150 r/min and constant temperature of 30 ℃ cultivated for 24 hours,the antagonistic energy of ferment filtrate was 1 500 IU/ml,the ferment filtrate showed strong heat stability, at 37 ℃ for 1 hour, the activity decreased by 35%; at 60 ℃ for 30 min, by 47%, for 1 h, by 50%, at 100 ℃,the activity maintained 39%.The optimum pH for the activity of the ferment filtrate was 7.0, at pH 3.0, the activity kept 70% and at pH 11.0, 65%.Conclusion:The fermentation filtrate of strain 1# of Bacillus licheniformis is antagonistic against S. mutans.

Objective To explore the cell-mediated immune function in patients with colon cancer undergoing minilaparotomy or laparoscopic assisted right hemicolectomy. Methods From January 2009 to August 2014, the colon cancer patients receiving right hemicolectomy were retrospectively analyzed. According to the operation mode, the patients were divided into minilaparotomy group and laparoscopic-assisted group. The clinical and pathological data was analyzed. Cell counts of total CD3, CD4, CD8, CD19 as well as NK cells in venous blood samples were compared between 1 day before surgery and postoperative days (POD) 1 and 5. Measurement data with normal distribution was compared using the t test or Q test. Count data was analyzed usingχ2 test or Fisher exact probability. Results There were 408 patients with colon cancer undergoing right hemicolectomy, 26 patients of whom were excluded. The remaining 382 patients were recruited in the research, which were divided into minilaparotomy group (182 cases) and laparoscopic-assisted group (200 cases). There was no significant difference in the age, gender, body mass index, TNM staging, histological type, blood loss, return of bowel function, tumor location, hospital stay and postoperative complications between the two groups (all P> 0.05). The operating time in minilaparotomy group [(131.53 ± 22.57) min] was shorter than that in laparoscopic-assisted group [(167.53 ± 22.04) min], and there was significant difference (t=15.76, P= 0.00). Compared with prior to surgery, cell numbers of CD3, CD4, CD8, CD19 and NK cells were lower on POD 1 and POD 5 (all P< 0.05), but there was no difference between minilaparotomy group and laparoscopic-assisted group (all P>0.05). Conclusion The minilaparotomy and laparoscopic-assisted right hemicolectomy have same effect on cellular immune function of patients with colon cancer.

Objective To investigate the expressions of ER? protein and ER? isoform mRNA in sigmoid colon of female slow transit constipation patients. Methods RT-PCR and Western blot were used to detect the expression of ER? protein and ER? isoform mRNA of 20 patients with STC and 20 age-matched controls. Results ER? protein and ER?1, ER?2 and ER?5 mRNA were detected in the two groups lower in STC group than that in control group (P