Invasion and metastasis are critical determinants on cancer morbidity.Genes and molecules participating in these steps have been demonstrated as potential prognostic factors.Growth factors and their receptors,cell-cycle regulators,cell-adhesion molecules and m trix-degrading enzymes are potentially used as prognostic factors.However,increased understandings for molecular mechanisms underlying carcinogenesis and its implementation in the treatment of gastric cancers have recently been focused on the development and incorporation of targeted agents with chemotherapy.

Objective To study the expression, distribution and significance of C2 gene in hepatocellular carcinoma (HCC) and gastric cancer tissue. Methods ABC immunohistochemical method was used to detect the expression of C2 protein in 60 samples of HCC tissue and 58 samples of gastric cancer tissue, and Western blot technique was used to detect the expression of C2 protein in 10 samples of HCC tissue and pericancerous tissue. Results 1. Expression of C2 protein in HCC tissue: in 60 samples of HCC and 42 samples of pericancerous tissue, the positivity rate of C2 protein was 27.3% and 83.3%, respectively. The positivity rate of C2 protein was significantly higher in pericancerous tissue than that in HCC tissue ( P

Objective To evaluate the therapeutic effect of 89 Sr internal radiotherapy on multiple-bone metastasis of cancer. Methods Forty-nine cancer patients with multiple-bone metastasis received 89 Sr internal radiotherapy. The pain control effect, life quality improvement, change in levels of blood calcium and serum alkaline phosphatase (ALP), and side effects were analyzed respectively. Results The total effective rate of pain control was 77.6 %. The life quality was improved obviously. The levels of blood calcium and ALP were decreased. No obvious side effects were found during the treatment. Conclusion 89 Sr internal radiotherapy had a good therapeutic effect on multiple-bone metastasis of cancer.

ObjectiveTo observe the effection of different interventions of group B streptococcus (GBS) infection in gestation period.MethodsOne hundred and seventeen cases with GBS infection were obtained from 1885 pregnant women,who were got routine prenatal examination at 34 to 37 weeks of pregnancy,and drug seeitivity test of secretions which were taken from under paragraph 1/3 of vagina,and divided into treatment group (91 cases) and untreatment group (26 cases).The treatment group was divided into treatment group one (47 cases) and treatment group two (44 cases).Treatment group one was treated with oral antibiotics for 7 days after diagnosis,treatment group one and two were treated with postpartum antibiotics intravenous infusion once every 4 hours in labor.Comparison of maternal and fetal outcomes.ResultsThe GBS infection rate was 6.2％ (117/1885).The morbidity of premature delivery,premature rupture of membrane and neonatal infection of treatment group [ 5.5％ ( 5/91 ),13.2％ ( 12/91 ),5.5％ (5/91 ) ]were lower than those of untreatment group [ 19.2％(5/26),30.8％(8/26),23.1％(6/26) ](P ＜ 0.05).The morbidity of premature dehvery and premature rupture of membrane of treatment group one[ 0,6.4％ (3/47)]were lower than those of treatment group two[ 11.4％ (5/44),20.5％ (9/44)](P ＜ 0.05).Conclusion Anti-GBS treatment can improve the outcomes of mothers and infants,especially early anti-GBS treatment during the period of pregnancy.

Through summarization on published literatures on allergic rat model in recent 20 years, domestic current research status and trend were analyzed. Some insights were provided on how to build a standard process for modeling and post-modeling evaluation. “Allergic rhinitis,” “rat model of allergic rhinitis”, or “animal experiment” was used as search terms. Related published articles were searched in the CNKI database from August, 1993 to October, 2013. Documents and data, including modeling method, drug dose, modeling cycle, model assessment described in the articles were extracted, summarized and quantitative analyzed. The results showed that 208 valid articles were included in this study. SD rat was used as animal model. Ovalbumin plus aluminum hydroxide were used as adjuvant sensitizing agents. Intraperitoneal injection plus nasal stimulation were the most commonly used modeling method. Behavioral evaluation scale score was used as the main evaluation index. It was concluded that currently there were many methods for allergic rhinitis animal modeling domestically. The post-modeling evaluation was relatively single. It was urgent and necessary to establish the standard process for modeling and post-modeling evaluation for allergic rhinitis animal model.

Objective To improve the deficiencies of current methods ,explore a new way to estimate endogenous antibodies in‐terference in immunoassay reagent .Methods According to EP14‐A3 ,RF samples and normal samples were tested at the same time by reference reagents ,reagent A and B respectively .Reagent A and B were to be evaluated .RF samples′location was compared to 95% CI of Deming regression line based on the normal samples .Results In comparison of reagent A vs .reference reagent ,RF sam‐ples exceeded 95% CI upper limit ,which indicated the anti‐interference ability to RF of reagent A was different from the reference reagent statistically .Meanwhile ,all RF samples tested by reagent B fell in 95% CI ,RF samples interfered reagent B hardly ,which indicated the reagent B had similar anti‐interference performance to RF as reference reagent .Conclusion The method from EP14‐A could intuitively reflect the resistance to endogenous antibodies for newly developed immunoassay reagents .

Objective To explore the correlation between cardiovascular risk factors and characteristics of coronary artery le‐sions ,the degree of stenosis in patients with coronary artery disease(CAD) .Methods Totally 156 patients who under went coro‐nary angiography(CAG) in our hospital were divided into CAD group(n=98) and non‐CAD group(n=58) which the patients with coronary stenosis <50% according to the result of CAG .Their clinical data were retrospectively analyzed ,including gender ,age , body mass index(BMI) ,family history ,smoking status ,left ventricular ejection fraction(LVEF) ,blood lipid ,blood pressure and blood sugar levels ,the correlation between cardiovascular risk factors and coronary artery lesions was analyzed .Results There was significant difference of gender ,systolic blood pressure (SBP) ,total cholesterol (TCH) ,three acyl glycerin (TG) ,low density lipo‐protein cholesterol (LDL‐C) ,fasting blood glucose (FBG) ,smoking between the two groups(P<0 .05) ,but no significant differ‐ence was found at the level of ages ,BMI ,high‐density lipoprotein cholesterol (HDL‐C) ,diastolic blood pressure (DBP) ,family his‐tory ,serum creatinine (Cr) ,LVEF between the two groups(P>0 .05) .The number of coronary artery lesion branch were increased with these risk factors incresed ,such as smoking ,SBP ,LDL‐C ,FBG ,no significant difference with age ,male ,TCH ,TG .The degree of stenosis were increased with SBP ,FBG ,no significant difference with age ,male ,smoking ,TCH ,TG and LDL‐C .Conclusion The smoking ,males ,FBP and SBP are the prominent risk factors of CAD in Qinghai area ,prosmote the occurrence and development of CAD together .

Purpose To investigate the effects of intervention with Tanakan on anterior ocular segment in diabetic retinopathy (DR) after retinal photocoagulation. Methods Prospective random controlled study was performed on 72 patients (72 eyes) with ultrasound biomicroscopy (UBM),by obtaining and quantitatively analyzing the changes of anterior ocular segment including anterior chamber, anterior chamber angle, ciliary body and choroids before and the 3rd day and the 7th day after retinal photocoagulation. Results Three days after photocoagulation, significant elevated IOP and narrowed chamber angle were observed in control group and 4 eyes (11.11%) in Tanakan group (P

[Objective]To study Bone microdmage to osteoavascular epiphysis caput humeralis in teenagers of the guts outbreak mechanism and make a diagnosis and give treatment in early stage important point.[Method]The review was from 138 patients of the shoulder department ache with shoulder joint movable function obstacle that orthopedics out-patient service of 2004~2005.There was X-rays / CT,the blood bio-chemical 5 example among the teenager of the patients,it was observed by medium short-term curative effect.We was combined a related cultural heritage study,put forward to make a diagnosis and gave treatment to have the instruction meaning to the clinic of four expect to categorize a method,and to early,medium,later period the non-surgical operation treatment observation.[Result]In 138 patients,there were 5 teenager patients who were the epiphysis of head humeralis bone microdamage ON,through the function instauration was normal:Earlier period 2 cases of 1~4 weeks,middle period 2 cases of 5~8 weeks,the later period 1 case of 4~6 months.We choose the medicine of prescription with the independence,"Huo xue hua yu","Bu shen zhuanggu","Tong luo zhi tong" medicinal broth,the right amount internal use outside use,having already obtained good result.We have already observated the serious bone arthritis/canceration,resulting in serious result(in another report).[Conclusion]Once or more shoulder was bone microdamage lead the caput epiphysis humeralis occurrence ON of guts.We observe the sufferer whom had the clinical symptom,physical sign,according to the clinical performance,course of disease's long/short,imageology atc.The suggestion is divided into early,medium,night and most late four periods,for the convenience of the choice treatment measure.Because of various reason,long and non-healing cases might develop to the most later period of case,such as the avascular osteo arthritis/canceration.

Objective To provide credible anatomic data and information for clinical diagnosis and surgery of hand by measuring the length of metacarpal bone and phalanges of hands on X-ray films and to study their mathematical relations.Methods The X-ray films of hands on posteroanterior position were taken in 186 healthy college students (M90,F96).The length of each metacarpal and phalange were measured.All the data were calculated by SPSS software.Results The mathematical relations of each metacarpal and phalange bone were obtained .It was found that there were sexual differences in the ratio of first to second,second to fourth,second to fifth and third to fourth metacarpal length,the ratio of first to second,first to fourth and second to fifth proximal phalangeal length,the ratio of second to fifth,third to fifth and fourth to fifth middle phalangeal length and the ratio of first to second and first to third distal phalangeal length.Conclusion The results of this mathematical relations are of great significant in orthopaedic surgery of hand.