Anticoagulants ; therapeutic use ; Child, Preschool ; Fontan Procedure ; adverse effects ; Heparin ; therapeutic use ; Humans ; Male ; Protein-Losing Enteropathies ; drug therapy ; etiology

Objective To identify factors influencing behavioral changes among injection drug users(IDUs)by assessing the outcome of the Kunming Sunshine Homeland Project(SHP)in 2007,so as to design more effective and relevant interventions for HIV/AIDS prevention and control in China.Method Data obtained from SHP were analyzed by Logistic regression,in which the relationship between related factors and effectiveness of the interventions were examined.Results Based on Fishbein and Guinan’s hypothesis(1996),among the three factors,i.e.attitude,perceived behavioral norm and self-efficacy,the perceived behavioral norm was the most effective one to change IDUs’ behaviors.Conclusions In designing HIV/AIDS prevention and control projects targeted to IDUs,perceived behavioral norms should be given high priority in combination with other 2 factors,and the approaches applied should match with the objective of the designed project.

OBJECTIVE:To observe long-term efficacy of intensive rosuvastatin therapy in the treatment of acute coronary syn-drome (ACS) and explore its possible mechanism,so as to provide reference for clinical diagnosis and treatment. METHODS:ACS patients receiving rosuvastatin after cured in our hospital during Jan. 2013-Jan. 2015 were divided into observation group(55 cases)and control group(47 cases)according to the dose of rosuvastatin. Under same guidance of physicans,2 groups were addi-tionally given Rosuvastatin calcium tablets(20 mg for observation group,10 mg for control group,qd,po)orally on the basis of routine treatment. Both groups were treated for 1 year. The incidence of 1-year accumulative main adverse cardiac event (MACE) were compared between 2 groups as well as the levels of blood lipid and serum cytokines,the occurrence of ADR before treat-ment,after 3 months of treatment. RESULTS:The incidence of accumulative MACE in observation group was significantly lower than control group,with statistical significance (P<0.05). After treatment,the levels of TC and LDL-C in 2 groups were de-creased significantly;the level of LDL-C was inCreased significantly compared to before treatment,and the observation group was significantly lower than the control group,with statistical significance(P<0.05). The levels of IL-1β and TNF-α in 2 groups were decreased significantly (P<0.05);the observation group was significantly lower than the control group,with statistical signifi-cance (P<0.05). The peripheral blood LDL-C was positively correlated with IL-1β and TNF-α in observation group after treat-ment,with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Intensive rosuvastatin therapy can significantly improve the long-term prognosis for ACS patients,which may associate with lipid metabolic regulation and anti-inflammatory effect.

Vertigo is a kind of patients' subjective feelings. The severity of vertigo is closely related to many factors. But we are short of a well accepted quantitative evaluation method capable of accurately and comprehensively evaluating vertigo in clinics. Reducing the onset of vertigo, enhancing the re- covery of equilibrium function, and improving the quality of life of vertigo patients should be taken as the focus of evaluating therapeutic effects. As for establishing a Chinese medical effectiveness evaluation system for vertigo, we believe we should distinguish different "diseases". We could roughly identify it as systemic vertigo and non-systemic vertigo. For systemic vertigo, the efficacy of vertigo could be comprehensively evaluated by UCLA vertigo questionnaire or dizziness handicap inventory combined with equilibrium function testing indices. But for non-systemic vertigo, the efficacy of vertigo could be comprehensively evaluated by taking UCLA vertigo questionnaire or dizziness handicap inventory as main efficacy indices. Secondly, we should analyze different reasons for vertigo, choose symptoms and signs in line with vertigo features as well as with Chinese medical theories, and formulate corresponding syndrome effectiveness standards according to different diseases. We should not simply take syndrome diagnosis standards as efficacy evaluation standards.
China ; Dizziness ; diagnosis ; Humans ; Quality of Life ; Surveys and Questionnaires ; Vertigo ; diagnosis

The number of nerve fibers in the spared dorsal root of the L4 spinal nerve and the number of nerve terminals in the Lamina Ⅲ of the segment of the spinal cord between L2 and S1 were determined with electron microscopic morphometry,and the distribution and density of nerve fibers in the spinal cord were observed with silver staining.It was found that the distribution and density of nerve fibers in the spinal cord showed no difference between the operated and the control sides under optical microscopy;morphometric analysis revealed a significant increase of the number of unmyelinated fibers and a tendency to increase of their diameter;and the number of both simple and complex terminals in the Lamina Ⅲ of the spinal cord could return to the normal level on the 35th day after operation with a significant increase of their diameter.The importance of these changes was preliminarily discussed.

AIM: To study if the effect of arsenic sulfide combined with IFN-α can be increased on K562 cells. METHODS: Telomerase activity was determined by PCRELISA. Flow cytometry was used to analyze the cell apoptosis. The final concentration of IFN-α and arsenic sulfide was 10 000 U/mL and 0.6 mg/L. RESULTS: The rates of apoptosis was 37.8% and 37% in K562 cells treated with IFN-α or arsenic sulfide alone for 8 days; The rates of apoptosis and inhibition of telomerase activity was 59.9% and 81.2% in K562 cells treated with IFN-α and arsenic sulfide simultaneously for 8 days, or 60.37% and 78.8% in K562 cells was treated with arsenic sulfide for 5 days after affected by IFN-α for 3 days. 71.3% telomerase activity was inhibited in K562 cells by arsenic sulfide alone for 8 days. CONCLUSION: Combination of arsenic sulfide and IFN-α can increase the apoptosis and inhibit the telomerase activity of K562 cells obviously comparing with the two drugs used alone. IFN-α maybe promote arsenic sulfide inducing apoptosis of K562 cells.

Objective To investigate the inhibitory effect on enhancement of contraction and relaxation functions of cardiomyocytes induced by of MN9202 TEA, Forskolin, and MB. Methods The twitch amplitude was measured with a video edge tracker method. The following indexes, including ph (peak height), peak height/baseline percent (ph/bl, %), maximal velocity of contraction (+dL/dt) and maximal velocity of diastolization (-dL/dt), were recorded by a computer. Results TEA, Forskolin and MB increased electrically-induced contraction as shown by the following indexes: ph, ph/bl%, +dL/dt and -dL/dt. Forskolin and MB markedly augmented function of cardiac myocyte contraction and diastolization. Comparing with the control group, Forskolin (10-8mol/L) increased ph from 0.12?0.03(um)to 0.24?0.06 (um), ph/bl % from 12?3% to 27?6%, +dL/dt from 1.8?0.5 (um/s) to 3.8?0.9 (um/s), -dL/dt from 1.8?0.22 (um/s) to 3.5?0.7 (um/s). These indexes increased by 91%, 123%, 110% and 89%, respectively. MB also increased significantly; ph, ph/bl %, +dL/dt and -dL/dt were 0.14?0.04um, 11?4%, 2.2?0.3um/s and 2.2?0.6um/s, respectively. MN9202 (3?10~ -6mol/L) decreased the indexes (+dL/dt and -dL/dt ) which were increased by Forskolin significantly. Conclusion TEA, Forskolin and MB increased function of cardiac myocyte contraction and diastolization. These drugs might elevate intracellular calcium content indirectly and directly. The effects of TEA, Forskolin and MB were attenuated by MN9202. MN9202 might not only block Dihydropyridine Receptor, but might also inhibit calcium influx. Further study is needed to elucidate exact mechanism of MN9202.

Transplantation of embryonic spinal cord was performed with microsurgical technique in rats after they were inflicted with acute spinal cord injury.Then the favorable factors affecting the acceptance of the transplanted cord were investigated with morphological technique.It was found that among the gestation ages from EH to E20,the spinal cord taken from the rats with the gestation age of E14 showed the highest survival rate of 70.83%.None of the transplants taken from the rats with the gestation age older than E14 survived.The connective tissue attached to transplants severely interfered with their acceptance and growth.The severity of the pathological manifestations of the injured spinal cord exerted unfavorable influence on the survival and growth of the transplants too.It is believed that in order to assure the success of the transplantation,besides the attention on the technical performance of the surgery,it is essential to determine the differentiation state of the embryonic spinal cord to be transplanted.

Objective To study the value of echocardiography in diagnosis of prenatal closure of fetal ductus arteriosus(FDA) and discuss its characteristics. Methods Two cases with prenatal closure and one case with constriction of the FDA were examined.The echocardiographic characteristics were analyzed and the literature was reviewed. Results Right atrial and ventricular dilatation and severe tricuspid valve insufficiency were observed. After timely delivery, impaired right ventricular function improved significantly. Conclusions Fetal echocardiography plays an important role in diagnosis of prenatal closure of the FDA.

Objective:With the development of science and technology, large medical equipments have been universally applied in clinical diagnosis. It is important in hospital management to search for the way to obtain the maximum economic and social benefits by efficiently analyzing cost benefit of large medical equipment. Methods:To ascertain cost benefit of medical equipment by profit margin of investments and cost-redemption periods. Results:Proposals on purchase and management of department medical equipment were presented. Conclusion:Scientific evaluation and analysis on large medical equipment would play a positive role in purchase, use and economic returns of department medical equipment.