Objective To observe the clinical efficacy ofZhibai Dihuang Decoction combined with endocrine therapy for kidney-yin deficiency type of advanced prostate cancer and its influence on patient symptoms of hot flashes.Methods Totally 72 cases of advanced prostate cancer patients were randomly divided into the combined group (36 cases) and the control group (36 cases). Patients in the control group were treated with endocrine therapy, while patients in the combined group were additionally treated byZhibai Dihuang Decoction. After 12 weeks of treatment, serum prostate specific antigen (PSA), the international prostate symptom score (I-PSS), the clinical symptoms score, the number of cases of hot flashes and safety index before and after treatment were observed. Results In the course of treatment, the control group and the combined group each had 1 case lost to follow up. PSA and I-PSS in the two groups significantly decreased after treatment (P<0.01). The total efficiency rate of TCM syndrome was 94.3% (33/35) in the combined group, while it was 57.1% (20/35) in the control group, with significant difference (P<0.01). After treatment, the incidence of hot flushes was reduced after treatment in the combined group, but the control group increased, with significant difference (P<0.01). No adverse reaction was found in the two groups.ConclusionZhibai Dihuang Decoction combined with endocrine therapy is more effective than endocrine therapy alone, which can reduce the symptoms of hot flashes.

Objective To study the urban elder people's cancer-spectrum in Chongqing from 1991 to 1998 in order to obtain the first-hand data and references for the prevention and the treatment of carcinoma in the seniles. Methods The data of population and death causes were collected with cluster sampling method in the communities of business, culture and industry in Chongqing. Their mortality rate, disease-specific mortality rate and the sequence of the death causes were analyzed. Results Their first death causes was cancer (mortality rate 811.95/105 persons, accounted for 21.25% of all death causes). The mortality rate of five cancers was as follows: lung cancer (282.45/105), esophagus cancer(119.47/105), liver cancer(108.41/105), stomach cancer (73.75/105) and intestine cancer (55.31/105). The prevalence of different cancer between male and female at different communiteis was different. Conclusions It is very important to strengthen the protection of the environment and advocate the healthy life mode in order to lower the mortality rate by cancer in urban seniles.

Objective To investigate clinical effects of transurethral resection of bladder tumor (TURBT) combined with postoperative perfusion of mitomycin C to urinary bladder on superficial bladder cancer. Methods 32 patients with superficial bladder cancer were treated by TURBT and discontinuous perfusion of mitomycin C (20mg/20ml) to urinary bladder. Results 32 cases of the superficial bladder cancers all were once ablated. 8 cases relapsed in 6 months to 2 years of postoperation, which were treated by TURBT and discontinuous perfusion of mitomycin C to urinary bladder again, and relapse was not found from postoperation to present. Conclusions Treatment of TURBT combined with perfusion of mitomycin C on superficial bladder cancer is repeatable, simple, safe and effective.

Adult ; China ; epidemiology ; Construction Industry ; Female ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; epidemiology ; Ships ; Welding ; Young Adult

Objective To investigate the expression and biological significance of microRNA-126 in colon cancer tissue.Methods The expression of microRNA-126 in colon cancer tissues and adjacent tissues of colon cancer patients was detected by real-time quantitative polymerase chain reaction (RT-PCR) in 104 patients with colon cancer surgery (total of 104 pairs).The lentiviral vector was used to construct microRNA-126 cell line,and the effects of microRNA-126 on proliferation,migration and invasion of colon cancer cells were further studied in vitro.Results The relative expression of microRNA-126 in colon cancer tissues (0.63±0.11) was significantly lower than that in adjacent tissues (1.08±0.15),the difference was statistically significant(t=14.561,P＜0.01).The positive rate of microRNA-126 expression in colon cancer tissues (61.5％) was significantly lower than that in adjacent cancer tissues (86.5 ％) the difference was statistically significant(x2=16.908,P＜0.05).The expression of microRNA-126 was significantly correlated with Dukes stage,depth of invasion,lymph node metastasis and distant metastasis (P＜0.05).In the Transwell experiment with matrix glue,the number of cell migration in transfection group (11.26±4.85) was significantly lower than that in blank control group (264.37±32.15),the difference was statistically significant (t=23.418,P＜0.01).In the Transwell experiment without matrix glue,the number of cell migration in transfection group (83.75 ± 13.74) was significantly lower than that in blank control group (339.64 ± 26.38),the difference was statistically significant(t=12.682,P＜0.01).MicroRNA-126 could inhibit the proliferation and invasion of SW480 cells.Conclusion MicroRNA-126 can significantly inhibit the development of colon cancer cells and affect the biological behavior of colon cancer cells.

OBJECTIVE To compare the clinical efficacy between adenoidectomy combined with auripuncture (AT+A) and adenoidectomy combined with tympanostomy tubes (AT+T) in children wiht otitis media with effusion. METHODS 92 cases of children with otitis media with effusion were selected from September 2010 to September 2014 in our hospital which was divided into AT+T group and AT+A group, depending on therapy methods.There were 48 in AT+T group and 44 in AT+A group. Retrospective analysis were made on a series of clinical data ,including the therapeutic effect, hearing threshold before and after treatment, infection rate and recurrence rate. RESULTS The hearing threshold has been significantly reduced within one year of follow-up after treatment,however,there was no significant difference (P>0.05). Total efficiency rate was 97.9% in AT+T group and 95.4% in AT+A group, no significant difference was found (P>0.05). The average middle ear fluid duration, infection and recurrence rate of children in AT+T group and AT+A group was (7.3±0.8) days, 6.3%, 4.2% and (11.7±0.4) days, 15.9%, 11.4% respectively, there was significant differences (P<0.05). CONCLUSION Both AT+T and AT +A have a good therapeutic effect for children with otitis media with effusion, which can significantly improve the hearing. Compared to AT+A, AT+T have a higher recovery rate,significantly reduce the recurrence and infection rate,and may be more suitable for clinical treatment of children with otitis media with effusion.

OBJECTIVE：To explore the possible mechanism of astragalus in improving cardiac function.METHODS：To observe the effects of astragalus,added to basic anti-heart-failure therapy,on cardiac function and TNF in 45 cases of thronic heart failure.RESULTS：In comparison with basic therapy group,the improvement of cardiac function was more obvious and the level of serum TNF-α was lower in astragalus group.CONCLUSION：Astragalus probably has the action of decreasing TNF resulting in improvement of cardiac function.

Objective To investigate the role of phosphatidyl-inositol 3-kinase-Akt (PI3k-Akt) signal pathway in the attenuation of ischemia-reperfusion (I/R) injury by sevoflurane preconditioning in isolated rat hearts. Methods Ninety-six adult male SD rats weighing 220-280 g were randomly divided into 6 groups ( n = 16 each): sham operation group (group S); I/R group; sevoflurane preconditioning group (group SP); wortmannin group (group W); dimethyl sulfoxide (DMSO) group (group D) and sevoflurane preconditioning + wortmannin group (group SW) . Their hearts were excised and perfused in a Langendorff apparatus with K-H solution saturated with 95%O2-5%C02 at 37 ℃ . The hearts were continuously perfused for 180 min in group S. After 15 min of equilibration, the isolated hearts were subjected to 30 min of ischemia followed by 120 min of reperfusion in SP, W, D and SW groups. Croups SP, W, D and SW received 10 min of perfusion with K-H solution containing 2. 4% sevoflurane, 100 nmol/L wortmannin, 20 μmol/L DMSO, and 2.4% sevoflurane + 100 nmol/L wortmannin, respectively, followed by 5 min washout before I/R. Eight hearts in each group were selected and HR, left ventricular end-diabetic pressure (LVEDP), left ventricular developed pressure (LVDP), and ± dp/dtmax were recorded at the end of equilibration and at 15 min of reperfusion, Myocardial tissues were obtained at 15 min of reperfusion for determination of apoptosis (by TUNEL) and phosphorylated Akt (p-Akt) expression (by Western blot) . Another 8 hearts were selected at 120 min of reperfusion for determination of myocardial infarct size by TTC staining. Result Compared with group S, LVDP and ± dp/dt,^ were significantly decreased and LVEDP was significantly increased in groups I/R, SP, W, D and SW, and myocardial p-Akt expression was up-regulated in groups I/R, SP and D ( P ＜ 0.05). Compared with group I/R, LVDP and ± dp/dtmax were significantly increased, LVEDP and apoptosis index were significantly decreased, myocardial p-Akt expression was up-regulated, and myocardial infarct size was significantly reduced in group SP (P ＜0.05) . Conclusion Activation of PI3K-Akt signal pathway is involved in the attenuation of I/R injury by sevoflurane reconditioning in isolated rat hearts.

Objective To establish a multi-drug resistant model of temporal lobe epilepsy and observe the effect of hippocampal stimulation on pharmacoresistant epileptic rats and its possible mechanism with the use of indexes including after discharges (AD) of the amygdalae,the stimulus-induced seizures and the extracellular levels of γ-aminobutyrate (GABA).Methods Totally,120 Wistar rats were used for the amygdaloid kindled model of epilepsy by chronic stimulation of amygaloid basal lateral nucleus.Based on the successful kindled model of epilepsy,we selected the pharmacoresistant and pharmacosensitive epileptic rats according to their response to phenobabital and phenytoin.We then divided the pharmacoresistant epileptic rats into the hippocampal stimulation group and control group,with 8 rats in each group.Low-frequency stimulus was conducted for two weeks in the hippocampal stimulation group.The hippocampus extracellular fluid collected by microdialysis was then used to determine the levels of GABA by a high performance liquid chromatography method after hippocampal stimulation.Results The stimulus-induced seizures were inhibited significantly,and the frequency of the AD was decreased,as well as the amplitude,compared with the control group.The extracellular level of GABA in the 8:00-9:00 am and the 8:00-9:00 pm was (32.69 ± 7.80) and (35.76 ± 6.27) μg/ml,respectively,both significantly increased as compared with the control ((26.58 ± 6.87) μg/ml,t =-21.45,P =0.000 ; (31.50 ± 4.87) μg/ml,t =-15.74,P =0.000).Conclusions The low-frequency hippocampal stimulation inhibits the seizures of the kindled model of epilepsy and decreases the frequency,the amplitude,as well as the duration of the amygdale AD in the pharmacoresistant epileptic rats,which may be contributed to the increased GABA content in extracellular fluid of the brain after stimulation.

Objective To build the county level hospital nursing assistance need indicator system in Jiangxi province.Methods Literature review,qualitative research method and Delphi expert consultation method were adopted to build the county level hospital nursing assistance need indicator system.The indicator system was revised through trial test.Results Two rounds of expert consultation showed that expert authority degree coefficients were 0.79,0.83; Coordination coefficient(Kendall's W) was 0.11～0.16; content experts consistency coefficient was 0.78,reliability Cronbach's α coefficient was 0.85.Through expert advice and pre-examinaion,the county level hospital nursing assistance need indicator system was built,consisting of 3 level Ⅰ indicators,8 level Ⅱ indicators,43 level ⅠⅡ indicators,including assistance contents,assistance ways and assistance time.Conclusions This study established the county level hospital nursing assistance need indicator system with better scientific and feasible characteristics.It can provide experience and guidance for the relevance and effectiveness of the implementation of follow-up care aid work.