AIM: To investigate the influence of microwave-assisted extraction on flavone contents of Pollen Tyhae micropowder. METHODS: Ultraviolet spectrophotometer and HPLC were applied to analyze Pollen Tyhae micropowder, total flavon and isorhamnetin-3-O-neohespridoside were adopted as the marker, respectively. RESULTS: Appropriate conditions of microwave-assisted extraction included: extraction time of 8min, ethanol concentration of 70%, Solid/liquid ratio of 1∶18 (g?mL -1) and the power of microwave oven of 540w. CONCLUSION: Compared with normal reflux method, microwave-assisted extraction of Pollen Typhae micropowder is more useful and can improve the extraction rate the reduce the extracting time.

With the increasing incidence and prevalence of male sexual dysfunction, andrologists are more and more in need of accurate and efficient tools to assess therapeutic efficacy and patients' satisfaction and to help patients achieve satisfactory treatment results. This article summarizes some of the most commonly used questionnaires for the diagnosis and assessment of the treatment of male sexual dysfunction, including International Index of Erectile Function (IIEF), Erection Hardness Score (EHS), Quality of Erection Questionnaire (QEQ), Erectile Dysfunction Inventory of Treatment Satisfaction (EDITS), Treatment Satisfaction Scale (TSS), Self-Esteem and Relationship (SEAR), Premature Ejaculation Profile (PEP), Premature Ejaculation Diagnostic Tool (PEDT), Index of Premature Ejaculation (IPE), Arabic Index of Premature Ejaculation (AIPE), Aging Male Symptoms Scale (AMS), Androgen Deficiency in the Aging Male (ADAM), and Symptomatic Inventory for Screening Late-Onset Hypogonadism in Males (SILOH), and presents an overview on their clinical application.
Erectile Dysfunction ; Humans ; Male ; Surveys and Questionnaires

Gold nanorods is a capsule-shaped gold nanoparticles. Gold nanorods give rise to two absorption peaks corresponding to their plasmonic modes, transverse mode and longitudinal mode, corresponding to light absorption and scattering along the short and long axis, respectively. The longitudinal surface plasmon resonance can be tuned by adjusting their aspect ratio from the visible to the NIR region and extremely sensitive to changes in the dielectric properties of the surroundings including solvents, adsorbates, and the interparticle distance of the gold nanorods. This unique optical property of gold nanorods opens up fascinating applications in biological and chemical sensors. Optical properties and biomedical application of gold nanorods are introduced, and its future research prospects are discussed.

Objective A retrospective study was carried out for the analysis of correlation of traditional Chinese medical syndrome patterns of asthma patients at period of onset with five-element motions and six climatic factors. Methods All of the subjects were the inpatients of the First Affiliated Hospital of Guangzhou University of Chinese Medicine admitted from January 20, 2011 to January 19, 2014, matching the year of Xinmao, Renchen, Guisi correspondingly in ancient Chinese calendar. The patients were confirmed as asthma at period of onset and were differentiated into various traditional Chinese medical syndrome patterns. The time of onset was classified into various Yunqi periods according to the theory of five-element motions and six climatic factors, and then the correlation of syndrome patterns with the Yunqi periods was analyzed. Results A total of 308 inpatient case files were collected. In the year of Xinmao (2011) , 31 cases were differentiated as cold pattern asthma, 48 as heat pattern asthma, 6 as turbidity pattern asthma, 5 as wind pattern asthma. In the year of Renchen (2012) , 37 cases were differentiated as cold pattern asthma, 58 as heat pattern asthma, 13 as turbidity pattern asthma, 7 as wind pattern asthma. In the year of Guisi (2013) , 32 cases were differentiated as cold pattern asthma, 47 as heat pattern asthma, 14 as turbidity pattern asthma, 10 as wind pattern asthma. The results of statistical analysis showed that the differences of the distribution of syndrome patterns were insignificant among the year of Xinmao, Renchen, Guisi (P>0.05) . During the 3 years, heat pattern asthma had the highest incidence, cold pattern asthma came next, and turbidity pattern asthma and wind pattern asthma came last. Distribution of syndrome patterns of asthma in the year of Xinmao was consistent with the law of five-element motions and six climatic factors of the year of Xinmao. On the aspects of the theory of six Jianqi ( climatic periods) , the distribution of patterns of asthma in the climatic periods of Chuzhiqi, Erzhiqi, Sizhiqi was consistent with the law of climatic periods, while that in the climatic periods of Sanzhiqi, Wuzhiqi, Liuzhiqi was not consistent with the law of climatic periods. In the year of Renchen, syndrome pattern distribution was not consistent with the law of five-element motions and six climatic factors of the year of Renchen. In the climatic periods of Chuzhiqi, Erzhiqi, Sanzhiqi, the distribution of patterns of asthma was not consistent with the law of climatic periods, while that in the climatic periods of Sizhiqi, Wuzhiqi, Liuzhiqi was consistent with the law of climatic periods. In Guisi year, the distribution of syndrome pattern was not consistent with the law of five-element motions and six climatic factors of Guisi year either. On the aspects of the theory of six Jianqi, except for Erzhiqi, the distribution of patterns of asthma in the other five climatic periods was consistent with the law of climatic periods. Conclusion The theory of five-element motions and six climatic factors has some significance in predicting the pathogenic characteristics of asthma and in guiding the prevention and diag nosis of asthma, but convinced conclusion needs more proof from large-sample clinical trial.

Objective:To explore the expression of PD-1 mRNA and PD-L1 mRNA in peripheral blood mononuclear cells(PBMCs)of OLP patients.Methods:The peripheral blood lymphocyte subsets (CD3 +,CD4 +,CD8 +,CD1 9 +,CD1 6 ++56 +)and humoral immunity indexes (lgG,lgA,lgM,C3,C4)were detected in 36 patients with OLP by flow cytometry and nephelometry respectively.The expres-sions of PD-1 mRNA and PD-L1 mRNA in PBMCs of 36 patients with OLP and 1 8 healthy individuals(the controls)were detected by fluorescence quantitative PCR,the correlation of PD-1 mRNA or PD-L1 mRNA with the immune function of OLP patients was analysed. Results:In OLP patients the percentages of peripheral blood lymphocyte subsets (CD3 +,CD4 +,CD8 +,CD1 9 +,CD1 6 + +56 +)as well as the complement of C3 and C4 were lower(P <0.05),the percentages of CD1 9 +(B)and the level of lgMwere higher(P <0.05),the expression levels of PD-1 mRNA and PD-L1 mRNA in rPBMCs were higher(P <0.05)than in the controls.There was positive correla-tion between PD-1 mRNA and PD-L1 mRNA expression level.The expression level of PD-1 mRNA was negatively correlated with CD4 +,but positively with lgG,the expression level of PD-L1 mRNA was positively correlated with CD1 9 +.Conclusion:The up-regu-lation of PD-1 mRNA and PD-L1 mRNA in PBMCs is correlated with the immunity of OLP patients.PD-1 /PD-L1 pathway may play an important role in immune pathogenesis of OLP.

OBJECTIVE:To improve the formulation of Kangfu cream,in order to increase the stability of it. METHODS:Ac-cording to the problem that demulsion of zinc oxide and poor stability of Kangfu cream,orthogonal test was used to optimize the formulation of cream matrix with oil phase ratio (stearic acid-white vaseline-glycerol monostearate),with the amount of polysor-bate 80,triethanolamine and SG-10 as factors,using comprehensive score of glossiness,delicacy,consistence,coating and stratifi-cation(10 points as full mark)as index. The stability of the cream was validated and investigated,such as compatibility,heat-re-sisting,cold-resistant,lighting. RESULTS:The formulation were as follows as 100 g cream containing stearic acid 8 g,white vase-line 8 g,glycerol monostearate 6 g,polysorbate 80 4 g,triethanolamine 3 g,SG-10 2 g and appropriate amount of water. The comprehensive scores of the cream were >9.5 points (n=3). Compared with the cream prepared by previous formulation,the cream prepared by modified formulation had no significant change in appearance and color,and demulsion and layering hadn’t been found. CONCLUSIONS:Kangfu cream with better stability than original formulation is successfully prepared.

Objective:To survey the status of anxiety/depression and lifestyle among junior high school students and explore their relation to put preference for psychological health education.Methods:Totally 1597 junior high school students aged 10 to 17 years in Shang Haicity were surveyed.The Generalized Anxiety Disorder-7 (GAD-7),Patient Health Questiormaire-9 items (PHQ-9) and Chinese Adolescent Lifestyle Scale (CALS),were used to assess anxious emotion,depressive emotion and lifestyle.Results:The rates of anxious emotion and depressive emotion were 16.4％ and 17.2％ respectively among students.Scores of GAD-7 and PHQ-9 were negatively correlated with all factor scores of CALS including eating habits,sleep status,health habits,learning habits,exercise,entertainment activities,interpersonal relationships,coping style and life satisfaction (-0.57 ≤ r ≤-0.15,Ps ＜ 0.01).Multivariate stepwise regression analysis showed that the sleep status factor scores,life satisfaction and exercise factor scores of CALS could negatively predict scores of GAD-7 (β3 =-0.17,-0.15,-0.05;Ps ＜ 0.05) and health habits positively predict scores of GAD-7 (β3 =0.08,P ＜0.001).Sleep status factor scores,life satisfaction factor scores and eating habits factor scores could negatively predict scores of PHQ-9 (β3 =-0.21,-0.16,-0.06;Ps ＜ 0.05).Conclusion:It suggests that the junior high school students'depression and anxiety are related to their lifestyle.The emotion of depression and anxiety of students may be predicted by some factors in the lifestyle.

Objective To observe the effects of lanthanum on learning and memory,and explore the corresponding mechanism on the basis of the levels of excitatory amino acids and calcium homeostasis of cerebral cortex in rats.Methods Forty female Wistar rats were randomly divided into control,low-,moderate-and high-dose lanthanum groups(ten rats in each group).The offspring of lanthanum exposure groups were administrated with lanthanum from weaning for one month.The capability of learning and memory was measured by neuroethology testing.The levels of excitatory amino acids,intracellular calcium ion and Nissl body expression of cerebral cortex were also detected.Results The capabilities of learning and memory in lanthanum exposure groups were significantly lower compared with the control,with a dose-dependent manner.The levels of glutamate and intracellular calcium ion of cerebral cortex in low-and moderate-dose group were significantly higher than those in controls,and the levels of glutamate,aspartate and intracellular calcium ion of cerebral cortex in high-dose group were increased significantly as compared with the control group,low dose group and moderate dose lanthanum group.Besides,Nissl body expression of cerebral cortex in low-dose group was lower than that in control group,and the levels of Nissl body expression of cerebral cortex in middle dose lanthanum group and high dose groups decreased further.Conclusion The increased levels of excitatory amino acids,calcium homeostasis unbalance and cellular injuries in cerebral cortex may be involved in lower capacity of learning and memory in the rats exposed to lanthanum.

Objective: To evaluate the malignant potential of oral lichen planus (OLP) by measuring and analyzing the dimensions of spinous cell in mucosal epithelium.Methods:Paraffin-embedded samples of oral mucosa, including normal (N,7 cases), OLP(16 cases), oral leukoplakia(12 cases) with mild and moderate epithelial dysplasia (LK) and well differentiated oral squamous cell carcinoma (SCC,10 cases), were analyzed by MIAS-99 System.The planar and stereoscopic dimensions of spinous cells were measured and compared.Results:The values of morphometric and stereologic parameters in OLP were between those in normal mucosa and in LK, significantly different from those in SCC and not significantly different from those in normal epithelium.Conclusion:Based on the changes of cell morphometric parameter value, OLP is generally a benign lesion with some degree of malignant potentiality that is less than LK.Nucleus/cytoplasm volume ratio and volume density of cell nucleus of spinous cells in mucosal epithelium are of value in distinguishing benign from maliganant lesions.

Objective:To explore the possible role and clinical significance of Th1 and Th2 class cytokines in the pathogenesis of re-current aphthous ulcer (RAU).Methods:35 cases with RAU,25 of minor RAU(MiRAU)and 1 0 of major RAU(MaRAU),were enrolled and 24 healthy subjects served as the controls.The expression levels of Th1 type cytokines (IFN-γ,TNF-α,IL-2)and Th2 type cytokines (IL-4,IL-6,IL-1 0)in the serum of peripheral blood were investigated by flow cytometry CBA methods and their rela-tionship with the clinical features of RAU was analyzed by SPSS 1 1 .5.Results:The expression level of IL-4 and IL-1 0 in MiRAU group was higher than that in the controls(P <0.05),and the expression level of IFN-γ,IL-4 and IL-1 0 in MaRAU group was higher than that in the controls(P <0.05).The expression level of IFN-γand IL-1 0 in MaRAU was higher than that in MiRAU.There was a positive correlation between the levels of IFN-γ,IL-4,IL-1 0 and the severity of RAU.Conclusion:Predominant Th2 immune re-sponse plays a role in RAU,and the level of IFN-γ,IL-4 and IL-1 0 is correlated with the severity of RAU.