Objective To establish a method for determination of new abused drugs in plasma by using ultra-high performance liquid chromatography tandem mass spectrometry ( UPLC-MS/MS ) combined with in-tube solid-phase microextraction. Methods 2 mL of borax buffer was added to 1 mL of plasma with drugs. Then,the mixture was withdrawn into a syringe connected to a stainless steel needle welded with DB-1701 capillary column. 50 μL acetonitrile was used as elution solvent at the flow rate of 300 μL·min-1 for 2 min. Subsequently,the drugs on elute were determined by using UPLC-MS/MS in positive mode with multiple reaction monitoring( MRM) to determine drug concentration in human plasma samples. ResultsThe recoveries rates of the five abused drugs ( codeine hydrochloride, tramadol, diphenoxylate hydrochloride, diazepam, and triazolam) in plasma were between 69. 2% -81. 7% with the RSDs less than 10%. LODs of the drugs were less than 25 ng·mL-1 . Seven out of the 26 plasma samples were determined positive. Conclusion The in-tube SPME method coupled to UPLC-MS/MS is simple, rapid, and environmental friendly, which is suitable for the the rapid screening of the new abused drugs.

Oestrogen(E), oestrogen receptor (ER) and oestrogen induced proteins form a cellular signal transduction pathway. As a member of this pathway, PS2 protein has close relations with gastric cancer. To elucidate its role in the course of tumourigenesis and advancing may contribute to the insight into the tumourigenic mechanisms and signal-transduction therapy of the gastric cancer.

Status epilepticus (SE) is one of the common medical emergencies of the pediatric age group.It may lead to serious and even irreversible neurological abnormalities,even death,if not diagnosis and treatment properly.To recognise etiology,rise factors,seizure type,treatment,outcome and the correlation between each other of children with status epilepticus are significant for management.This paper aimed to review recent epidemiological studies on children with status epilepticus,in order to provide evidences for the current clinical practice and further research.

OBJECTIVE To compare the monitoring effect of 2 kinds of B-D testing package.METHODS 2 kinds of testing package around the air vent of first pot sterilizer before antiseptic every day were used preheating 5 min.Start to move the B-D procedure when the band pressure to achieve above 100 kPa with 3 time of pulsation vacuum,inner chamber pressure of 210 kPa,the temperature of 134 ℃ and time of 3.5 min.RESULTS Except the false negative caused by package factor,the testing accurate rate of disposable B-D testing package was 100%.But the testing accurate rate of repeated uses B-D testing package was 90%.CONCLUSIONS The disposable B-D test package may respond promptly the breakdown of sterilizer and avoid the false negative result which the artificial pack factor creates.The test result is accurate.The disposable B-D testing package for examination of autoclave exhaust cold air degree is obviously better than the self-made cotton fabric testing package.

Mycoplasma, Chlamydia, Rickettsia, Spirochetes and Actinobacteria were generally called "four pathogens and one bacterium". It was always difficult to be denominated and classified rightly in textbooks, while it was also a key interfering with students to grasp the concept of bacteria exactly. So we raise the question and hope to learn from each other by an exchange of views here.

Yersinia enterocolitica,which is pertaining to pathogenetic Yersinia,is a human and animal pathogen found in most countries of the world.The microorganism is widely distributed in nature in aquatic and animals.It is transmitted through the respiratory tract or digestive tract.The epidemical seasons are mainly autumn and winter.Within a broad spectrum of clinical diseases,gastroenteritis is commonly encountered.To detect Yersinia enterocolitica,molecular biological method is the highest sensitive and special.

The mammalian TOR ( mTOR ) pathway is a key regulator of cell growth and proliferation which is also a fundamentally important player in a large variety of human diseases. The pathway regulates many key cellular processes and is implicated in a large number of pathological conditions, including cancer, obesity, diabetes, and cardiovascular disease, age - related diseases and other proliferative disease. It integrates signals from nutrients, energy status and growth factors to regulate many processes, including autophagy, ribosome biogenesis and metabolism. Our article reviews the advancement of laboratory results on mTOR pathway in proliferative diseases in ophthalmology.

It has been demonstrated by our previous research that 4-(2-acetoxybenzoylamino) butyramide derivatives exhibited good antiepileptic activities. In this paper, to explore the SAR and improve the antiepileptic activities of these derivatives, a series of novel 4-(2-acetoxybenzoylamino) butyramide heterocyclic compounds (5a-5n) were synthesized and biologically evaluated. Their structures were confirmed by 1H MNR, ESI-MS and elemental analysis. Pharmacological test in vivo showed that target compounds (5f, 5i-5n) displayed strong antiepileptic activities on 4-AP induced epilepsy in mice with ED50 values ranging from 0.3137 to 0.3604 mmol x kg(-1).

AIM To study the relation between the antiarrthymic effect and the inhibition of slow reaction cardiomyocyte of ethacizine(EC). METHODS The beating rate and action potentials of cultured slow response cardiomyocyte from neonatal rats was recorded. RESULTS EC 0 1～5 0 ?mol?L -1 produced a concentration dependent suppressing effect on spontaneous beating rate of cultured cardiac cells with IC 50 of 0 17 ?mol?L -1 and the maximal inhibitory rate was 52%.The steady state of inhibition to beating rate was achieved after adding EC for 15～30 min. The effective inhibition of beating rate by EC lasted for over 1 h.As EC 2 5 ?mol?L -1 depressed beating rate to the steady state ,effects of CaCl 2 2 mmol?L -1 and Iso 1 ?mol?L -1 on beating rate were partially reduced,and the effect of aconitine 0 2 mg?L -1 was abolished. EC 0 3～2 5 ?mol?L -1 pruduced the concentration dependent depression on APA,V max ,OS,SP 4 and MDP,and prolongation of SCL of the slow response action potentials.APD 90 was also lengthened at the concentration of EC 0 9～2 5 ?mol?L -1 . CONCLUSIONS The effective depression of EC on abnormal automaticity and conductance of the slow response cardiomyocyte in heart ischemic zone may be an important mechanism for its effective termination on some arrhythimas.EC may depress Ca 2+ ,especially Na + currents on slow response cardiomyocyte.

Objective:To explore the potential targets for Traditional Chinese Medical Formula against tuberculosis infection.Methods:U937 cells were incubated with the drug-serum prepared with Clehnia root Ophioponis Decoction(COD),Lily Metal-Consolidating Decoction (LMCD) and Radix Ginseng Gecko Powder(RGGP) for 24 hours.The mRNA and protein level of CR1,CR3,CD14,CD43,TLR2 and TLR4 expressed in macrophages respectively were then measured by RT-PCR and FACS.The phagocytic and killing activity of the U937 cells interfered with the drug-serum for 24 h were then detected after BCG stimulation.Meanwhile,secretion of IL-10,IL-12 and the activity of iNOS in the macrophages were detected by ELISA and biochemistry method in each group.Results:The drug-serum prepared with COD,LMCD and RGGP had different regulatory effects on the expression of PRRs and the production of IL-10 and IL-12 in the macrophages,and could enhance the phagocytosis percentage and phagocytic index(P