The use ofYang transforming Qi, Yin constituting the bodycan be used in the treatment of metastasis of breast cancer from pathogenesis transformation view. Based on literature, this article analyzed the theory of Yang transforming Qi, Yin constituting the body and its possible guidance to clinical practice. Then the article illustrated the application cases of this theory in the treatment of breast cancer metastasis.

Objective To improve the poor water-solubility of nimodipine,nimodipine-loaded PLEG copoly- met nanopartieles were prepared to avoid the sedimentation of its crystal from water,which could also shun the blood vessel stimulation caused by ethanol,a cosolvent,in the injection.Methods Solvent evaporation method was used to prepare water-dispersing nimodipine-loaded nanoparticles,whose lyophilized powder was further prepared,with PLEG2000 as drug carrier.Particle size,Zeta potential,embedding ratio,drug loading,shape and redispersion ability of the lyophilized powder were determined.Results Concerning the lyophilized nanoparticle powders,the particle sizes were 136nm,the Zeta potentials were -29.90mV,the embedding ratio was 67.9% and the drug loading was 6.17%.Conclusion Prepared nimodipine-loaded nanoparticles solved the problem of poor water-solubility of ni- modipine basically.It also avoided the recrystallization process of the drug and reduced the blood vessel stimulation caused by ethanol in the injection.

Objective To analyze the situation and trend of the application of antibacterial drugs in the case of typeⅠincision operation in our hospital.Methods A total of 1 964 patients in our hospital from 2010 to 2014 were randomly selected,and the changes of antimicrobi-al drugs during the treatment period were analyzed.The surgical site infection probability and antimicrobial application rules in typeⅠincision surgery in 3 years,including probability,category,first time and duration of drug use,and the relationship between antibacterial drugs and typeⅠincision infection were analyzed.Results Application of antibacterial drugs in typeⅠincision operation probability decreased significant-ly,and the surgical infection probability decreased year by year,with statistically significant difference(P<0.05).The first application of an-tibacterial drug time was more reasonable,the choice of antimicrobial drugs were mainly one generation or two generation of Cephalosporin. There was a significant increase in the probability of stopping medication within 2 days after surgery,and the difference was statistically signif-icant(P<0.05).Conclusion The application of antibiotics should be strictly applied to the application of the indications for the use of anti-biotics.To understand the scope of application of antimicrobial drugs,and should try to shorten the use of time,control of infection during peri-operative period,reduce drug resistance.

Fibroblast growth factor 23 (FGF23) is a new endocrine factor, mainly expressed in osteoblasts and osteoblasts. Many studies have found that FGF23 can act on kidney, parathyroid and other tissues after binding with Klotho protein, and participate in bone mineral metabolism. In addition, in recent years, the role of FGF23 in exoskeleton has been gradually discovered, such as FGF23 and thyroid diseases, diabetes and so on. Therefore, understanding the biological characteristics, regulatory mechanism of FGF23 and its relationship with related diseases is of great clinical significance for the diagnosis and treatment of diseases. This article reviews the relationship between FGF23 and metabolic diseases such as phosphorus metabolic disease, parathyroid disease, hyperthyroidism, osteoporosis, diabetes, iron metabolism and so on.

Objective To observe the effect of puerarin on the level of malondialdehyde (MDA) and myeloperoxidase (MPO) in myocardial in scalded rat.Methods Eighty-eight Wistar rats were randomly divided into the recovery group (group R,n=40),the treatment group (group T,n=40) and the control group (group C,n=8) . The rats in the recovery and the treatment groups were subjected to 30% TBSAⅢdegree scald.Myocardial tissue samples from the group R and group T were harvested at 1,3,6,12,24 postburn hours for the determination of MDA and MPO. The morphological change in the myocardial tissue was observed with transmission electronic microscope.Results (1)In group R,MDA、MPO went up 1 hours after burns,and all attaining the top at 12 hours post burn (P＜0.01).(2)In group T,the indexes above had the same trends as group R.But comparing with group R,MPO and MDA were much lower at 1,3,6,12 hours (P＜0.05 or P＜0.01).(3)Comparing with group R,the altrastructural changes were obviously alleviated at 24 hours in group T.Conclusion The production of MDA,MPO in severely burned rats can be inhibited by puerarin,which was beneficial in the management of myocardial injuries after severe burns.

Chromatography, High Pressure Liquid ; methods ; Humans ; Neonicotinoids ; Pyridines ; blood

Chromatography, Gas ; methods ; Humans ; Paraquat ; analogs & derivatives ; blood

OBJECTIVE To investigate the effects and its underlying mechanis m of bisphenol-A (BPA)exposure on spine and synapse formation in detate gyrus (DG)area of hippoca mpus during criti-cal develop mental period.METHODS Sprague-Dawley(SD)rats were injected intraperitoneally with BPA (50,250 and 500 μg·kg -1·d -1 )fro m postnatal day 7 (PND7)to PND14.Dendritic spine morphol-ogy in DG area was exa mined using Golgi-Cox staining method and determined with I mage J software. Western blotting method was e mployed to test the Wnt related proteins.RESULTS The spine density and the average spine head size in BPA exposed groups significantly decreased in a dose-dependent manner when co mpared to control group(P<0.05).Meanwhile,Wnt related proteins were affected dur-ing BPA exposure.Specifically,the percentage of phosphorylated β-catenin increased following BPA ex-posure (P<0.05),whereas Wnt7a expression level was significantly decreased and Wnt5a expression level increased (P<0.05).CONCLUSION Wnt signaling pathway plays an i mportant role in BPA-in-duced i mpairments in spine and synapse formation.

With the rapid development of network technology,the data sharing and health research provides an efficient and convenient way for medical research and clinical guidance.In order to know patients' opinions on informed consent and data sharing,we investigated first-class hospitals in the coastal and inland areas with a total of 397 patients participating the questionnaire on clinical health and data sharing,reusing the remaining clinical blood samples in scientific researches,and the acceptance degree of broad consent investigation and related influencing factors.It was found that patients with strong consciousness of informed consent right tended to choose a simpler way of informed consent based on the analysis.Patients preferred social benefits than personal benefits with a strong spirit of dedication to the community.Medical institutions in China rarely applied broad consents and took relervant summative researhes,compared with the European and American countries' promotions at the national level.The research afforded the evidence,from most of patients' point of view to ethics committee in the review of such kind of studies to understand the public data sharing in privacy protection and broad consent of the individual opinion,in order to facilitate the rational development and promotion of health data sharing in China ethically.

Objective; To investigate the remodeling mechanism of the retrodiscal tissues of temporomandibular joint after mechanical loading. Methods; 15 adult New Zealand rabbits were subjected to traction between the mandibular ramus and zygomatic arch in the postero-superior direction unilaterally using elastic force. The animals were killed respectively at 2, 4 and 6 weeks. The histologic features were observed by Hematoxylin & Eosin and Alcian blue staining. The expressions of aggre can and collagen II antigen in the articular disc of temporomandibular joint were observed by immunohistochemical staining. Results: The articular disc of the traction side in temporomandibular joint was characterized by an early phase (2 weeks postoperatively) with partial anterior disc displacement and disc deformity and subsequently in deposition of fibrous material in the matrix of the retrodiscal tissues. With time going on, fibroblasts significantly decreased and connective tissues increased. A small quantity of cartilage-like cells were also investigated in that area. However, no obvious histological change was observed in the control group. Compared with the control group, glycosaminoglycan, ag-grecan and collagen II were weakly positive in the intermediate zone of articular disc in experimental group. Aggrecan was stronger positive in the intermediate zone of articular disc after two weeks in experimental group. When traction period prolonged, glycosaminoglycan, aggrecan and collagen II were strong positive in the retrodiscal tissue of articular disc in experimental group. Conclusion; These investigations reveal that distracting mandibule towards rear and top through ansa capitis, the synthesis of cartilage matrix increase and a small quantity of cartilage cells appear to adjust to its functions.