Objective To study the distribution and drug‐resistance of pathogens isolated from vaginal secretion in the patients with vaginitis in Dalian area so as to provide an evidence for clinical diagnosis and treatment .Methods The statistical analysis was performed on the detection results of pathogens isolated from vaginal secretion in 5 308 patients with vaginitis .Results In 5 308 patients with vaginitis ,the routine detection of vaginal secretion found that 810 cases(15 .3% ) were bacterial vaginosis ,1 110 cases (20 .9% ) were candida positive and 60 cases (1 .1% ) were trichomoniasis positive ,in which 3 526 cases were performed the patho‐gen detection ,2 615 strains of pathogen were isolated ,including 1 948 strains (74 .5% ) of mycoplasma ,136 strains (5 .2% ) of Chlamydia ,17 strains (0 .7% ) of Neisseria gonorrhoeae ,276 strains(10 .6% ) of candida ,140 strains (5 .4% ) of Gram‐positive cocci and 98 strains(3 .7% ) of Gram‐negative bacilli .The susceptibility rates of Ureaplasma urealyticum to ofloxacin ,levofloxacin ,spar‐floxacin and roxithromycin were lower ,which were 7 .8% ,18 .2% ,26 .3% and 27 .2% respectively ,the susceptibility rates to clar‐ithromycin ,doxycycline and minocycline were higher ,which were 89 .4% ,99 .8% and 99 .9% respectively .The drug resistance of Mycoplasma humenis was higher .The more sensitive antibacterial drugs were only josamycin ,doxycycline and minocycline ,the sen‐sitivity rates were 75 .3% ,96 .2% and 97 .3% respectively ,which to other antibacterial drugs were less than 7 .0% .Candida albi‐cans was generally sensitive to antifungal drugs .The susceptibility rates of Staphylococcus aureus to commonly used antibacterial drugs were higher .All of 24 strains of Streptococcus agalactiae were sensitive to penicillin .No Gram‐positive coccus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to imipenem and meropenem was found .Conclusion The pathogens causing vaginitis are very complex .In the treatment of female vaginitis ,clinicians should rationally use antibacterial drugs according to the results of pathogens detection and drug susceptibility test .

Objective To detect the expression of mutational p53 and inducible nitric oxide synthase in ovarian neoplasm and study the correlation between them. Methods The expression of p53 gene and inducible nitric oxide synthase was detected by immunohistochemistry method. Results ①The expression of p53、iNOS in healthy ovary,benigh and malignant ovarian tumor increased gradually(909%、13.13%、65.31%;18.18%、20.00%、61.22%) (P

Objective:To investigate the clinical features,pathological changes of 34 cases that had suffered infantile hepatic glycogen storage disease in order to improve the recognition.Methods:The clinical manifestations of 34 cases of infantile hepatic glycogen storage disease during May 2005 to May 2009 were collected.The hepatic tissues from liver biopsy were observed under microscope and electron microscope.Results:Hepatomegaly and growth retardation were the main clinical manifestations of hepatic glycogen storage disease, partial cases were complicated with the manifestations of hypoglycemia.Hypoglycemia,hyperlactacidemia and abnormality of liver function were detected.And the pathology features were observed under microscope and electron microscope.Conclusion:The clinical manifestations of infantile hepatic glycogen storage disease were common,and the final diagnosis can depende on liver biopsy.

microRNA (miRNA) is a class of endogenous small non-coding RNA (20-25 nt) which can regulate the expression of protein-coding genes post-transcriptionally and participate in a series of critical biological processes including cell proliferation,differentiation,metabolism,apoptosis,et al.Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma subtype.DLBCL is defined as the malignant lymphoma which originates from the B cells,with aggressive clinical behaviors and significant clinical heterogeneity.In recent years,the researches on miRNA in DLBCL have shown that several kinds of miRNA directly involved in the occurrence and development of tumors and there is a correlation between the miRNA expression profiles and molecular subtypes of DLBCL.As a kind of new molecular target,miRNA has a broad prospect in the application both for diagnosis and biotherapy of DLBCL.

Objective To detect the expression of mutational p53 and inducible nitric oxide synthase(iNOS) in ovarian neoplasm,and study the correlation between them. Methods The expression of p53 gene and inducible nitric oxide synthase was detected by immunohistochemistry method.Results(1)The expression of p53、iNOS in healthy ovary,benigh and malignant ovarian tumor increased gradually(9.09%,13.13%,65.31%;18.18%,20.00%,61.22%) P

Objective] The article summarizes the experience of using sanren decoction to treat pediatric diseases by Professor Liu Lin, the famous TCM physician of Hubei. [Methods] Through following Professor Liu Lin clinical diagnosis, organize his medical records, summarize the experience and thoughts of using Sanren decoction to treat pediatric diseases, and for some cases. [Results] Professor Liu Lin believes that the physiological and pathological characteristics of children prone to cause hot and humid accumulation, pediatric Chih-Yin is not substantial, with their lungs often delicate and spleens often inadequate, they prone to get hot and humid accumulation after sick, and later appear a variety of symptoms including upper burner, center burner and lower burner. Therefore, the treatment of pediatric disease should take the heat into account, bearing in mind the residence site of heat evil and syndrome differentiation. Professor Liu Lin makes good use of Sanren decoction to treat a variety of pediatric diseases, judging the residence site of heat evil and clinical manifestations, giving the corresponding treatment flexibly. Such as the treatment of bronchitis with almonds as the monarch herb to expectorant cough, treatment of infantile anorexia with White Kou Ren as the monarch herb to activate spleen and remove dampness, treatment of children with nephrotic syndrome with Yiyiren as the monarch herb for penetration of water, treatment of HFMD with the Sanren to regulate Qi of triple burner, according to the symptoms to medicate and always has good clinical effect. [Conclusion] Professor Liu Lin using Sanren decoction to treat pediatric diseases is effective, his thought is worthy of depth study and promotion.

Objective:To study the inhibitory effects of adenovirus mediated vascular endothelial cell growth inhibitor 151(VEGI_(151)) on venous endothelial cell proliferation. Methods: VEGI_(151) gene was cloned into adenovirus vector pCA13 and the product was packaged and amplified with 293A cells.The expressed protein was identified by Western blot. X-gal staining was used to determine the transfection efficiency of the recombinant adenovirus system. The inhibitory effect of Ad-VEGI_(151) on ECV304 cell proliferarion was observed and assessed by violet crystal assay. The protein expression of VEGI_(151) gene in ECV304 cells was detected by immunohistochemistry. Results: Liposome-mediated pCA13-VEGI_(151) and pJM17 cotransfection of 293A cells (through homologous recombination of intracellular plasmid) is a practical and feasible method to obtain high-titre recombinant adenovirus. The constructed Ad-VEGI_(151), with high transfection efficiency, significantly inhibited ECV304 cells proliferation and expressed functional protein in target cells. Conclusion: Ad-VEGI_(151) can express bioactive protein in target cells and can inhibit the proliferation of venous endothelial cells, and this may pave a new way for gene therapy of tumors and neovascular diseases.

Parathyroid carcinoma is a rare endocrine tumor.Clinical manifestations are varied with multiple organs involved,mainly due to hypercalcemia.So far the etiology and pathogenesis of parathyroid carcinoma are still unclear and preoperatively diagnosis is difficult.Technetium-99 m sestamibi scan and ultrasound can help to find the early lesions.Complete surgical resection with suspicious involved lymph nodes is the recommended treatment.Parathyroid carcinoma is insensitive to chemotherapy and radiotherapy.New immune therapy yields unsure efficacy.Therapeutic effect and prognosis mainly depend on primary surgical resection of the tumor.The disease recurs in more than 50％ of patients with poor prognosis.

OBJECTIVES:To isolate and purify a new peptide named as NV-PP-1,from anti-inflammatory protein extracts of Nidus Vespae and to characterize it.METHODS:It was purified by ammonium sulfate precipitation,DEAE-Sephadex A50 ionexchange chromatography,Sephadex G-50 gel filtration and HPLC，respectively.Then it was characterized by HPLC on Protein-PakTM-60 column,IEF-HPCE(isoelectric focusing-high performance capillary electrophoresis),UV spectrum,amino acid analysis,and the N-terminal analysis with DNS-Cl.RESULT:Both HPLC on Protein-PakTM-60 column and IEF-HPCE showed its homogeneity.Its molecular weight was estimated to be 7.079 kD by HPSEC.UV spectrum indicated its maximum absorption at 274nm.Its PI was determined to be 4.08 according to IEF-HPCE.The terminal analysis showed its N-terminal amino acid was blocked.Amino acid analysis of NV-PP-1 indicated that it was rich in Ser,Glu,Gly.CONCLUSION:NV-PP-1 is the first peptide isolated from the anti-inflammatory protein extracts of Nidus vespae.

Objective To investigate the effect and psychological intervention of the treatment of hemodialysis internal fistula with Hirudoid combined with far infrared ray. Methods 80 cases of hemodialysis fistula were diagnosed and treated from January 2014 to December 2016 in Lishui Central Hospital,according to different treatment intervention methods, which were divided into two groups, each group with 40 cases. The control group was treated with routine nursing and far infrared therapy, and the observation group was treated withpsychological intervention and Hirudoid+far infrared therapy. The clinical efficacy, the improvement of clinical symptoms and the incidence of complications were compared between the 2 groups. Results The clinical effect of the observation group was better than that of the control group, the improvement of the clinical symptoms was better than that of the control group,the complication rate was lower than the control group,the difference was statistically significant (P<0.05). Conclusion The clinical effect of psychological intervention plus Hirudoid+far infrared ray in the treatment of hemodialysis fistula patients is significant,which can effectively improve the clinical symptoms and complications in patients with less, and is worthy of clinical application.