BACKGROUND: Edema and systemic disorder of energy metabolism after spinal cord injury can induce a series of amplifying effect, which can deterio rate the injured level. Matrix metalloproteinase-9 (MMP-9) is able to digest the basal lamina and damage the close joint of capillary vessel by proteolysis,which induces blood-brain barrier breakdown and brain edema formation.OBJECTIVE: To investigate the spatial characteristic of the MMP-9 in acute injured spinal cord so as to investigate the effect of MMP-9 on secondary spinal injury.DESIGN: A completely randomized study.MATERIALS: The study was completed in the Department of Pathology,Medical College of Wuhan University. Totally 40 healthy male Sprague Dawley rats were divided randomly into control group and model group.Five rats in control group were treated with laminectomy. Other 35 rats in model group was used to establish model of spinal cord injury.METHODS: After anesthetized, complete spinal cord tissue was taken out. Tissue of T8.9 spinal cord was cut out and every 3 serial sections of each tissue were taken out randomly for immunochemical analysis of MMP9 expression. Spinal cord tissue at injured segment was taken with 5 rats at each time point. Five visual fields (×400) of every slice were gathered randomly in injured area (sham-operation region in control group), average absorbency was determined then.MAIN OUTCOME MEASURES: Expression of MMP-9 in the acute in jured tissue of spinal cord.RESULTS: Totally 40 rats entered the final analysis. The expression of MMP-9 was observed 1 hour in model group with the peak time of 1 to 2 days and also seen on 7 days. The MMP-9 positive cells were mainly expressed on ischemic neuron, vascular endothelial cells and neutrophilic granulocyte. MMP-9 was not assayed in the control group.CONCLUSION: The early expression of MMP-9 and the characteristics of spatial distribution after spinal cord injury play a key role in tissue edema and inflammatory cell infiltration.

Objective Use the automatic microbiological analysis system which for the streptococci (ATB rapid 32 Strep) to identify streptococci, and compare the examination speed and accuracy with the internationally recognized bacterium identification golden method API 20 Strep (streptococci identification biochemistry system) to enable the clinical microorganism room use ATB rapid 32 Strep identification system correctly, and provide the etiology basis to clinical promptly.Methods Isolate the bacterium from patient blood, cerebrospinal fluid, pig heart, pork and so on, then separate the streptococci in form to the blood plate, lay them in carbon dioxide incubator, after 35 ℃ 24 hours. Use ATB rapid 32 Strep,API 20 Strep and the traditional manual method to identify 18 strains of bacterias and carry on the medicine sensitive experiment.Results ATB rapid 32 Strep identified 16 strains as streptococcus suis II, 2 strains as streptococcus bovis II; API 20 Strep identified 17 strains as streptococcus suis II, 1 strain can not be identified; the tradition manually method identified all of the 18 strains as streptococcus group D . These 18 strains all sensitive to the Chloramphenicol , clindamycin, erythromycin, levofloxacin, penicillin, quinupristin/dalfopristin, cefotaxime, vancomycin and sulfafurazole , and resistant to the tetracyclin.Conclusion ATB rapid 32 Strep can identify the streptococcus suis in 4 hours , and the coincidence rate with API 20 Strep is 94.4%.So ATB rapid 32 Strep is one kind of system which could identify bacterium simply,quickly,accurately,and provide infectious disease etiology information for clinical immediately, satisfy the requirement of clinician and the microorganism examines.

Background Keratectasia after laser in situ keratomileusis (LASIK) is one of the serious complications which affect the vision.The measurement of cornea posterior elevation is an important way helping to find this change.Objective The aim of this study was to investigate the change of cornea posterior elevation after LASIK in myopic eyes with astigmatism and affecting factors.Methods A series case-observational study was adopted.One hundred and twenty-seven myopic eyes with astigmatism of 66 patients who received LASIK in 2008 May through 2010 January in Beijing Tongren Hospital were retrospectively analyzed to evaluate the change of cornea posterior elevation following the LASIK.The parameters related to cornea posterior elevation were measured and compared before and 3 months,6 months and 1 year after surgery,respectively,with Oculyzer anterial segment analysis system.Results The height values from posterior cornea highest point,the lowest point and central vertex central elevation zone were (12.20±3.39),(-19.02±7.38) and (1.05 ±3.25) μm respectively before LASIK and were (14.38±3.80),(-18.55±7.11),(2.83±4.81)μm in3 months and (13.99±3.38),(-17.57±6.54),(2.45±4.61) μm in 6 months after LASIK.They were (14.40±3.85),(-17.76±6.00),(2.16±5.00) μm in 1 year after surgery.Significant increases were found in the vertex height and central elevation after LASIK compared with before surgery(highest pointq=6.813,5.594,6.875,all at P＜0.001.central vertex:q=4.488,P=0.002;q =3.530,P=0.013 ;q =2.799,P =0.047).However,no significant difference was seen in various time points after LASIK (P＞0.05).A positive correlation was obtained between the height value of the posterior cornea central vertex with the spherical equivalent (SE),maximum cutting depth or cutting proportion 1 year after LASIK (r =0.295,0.297,0.295,all at P=0.001),and there was a negative correlation between it with residual stroma (r=-0.208,P=0.019).The intraocular pressure (IOP) measured with noncontact tonometry (NCT) was (14.24±3.33) mmHg before LASIK,and those of 3 months,6 months and 1 year were (8.42± 1.90),(8.61 ± 1.64) and (8.76± 1.64)mmHg after LASIK,showing a significant lowing in IOP after LASIK in comparison with before LASIK (q =29.851,28.317,26.337,all at P＜0.001).But no significant change was found in the different time points after LASIK (P＞0.05).There was significant difference in the IOP corrected by Ehlers after LASIK (P＞0.05).The IOP before LASIK had positive correlation with the central elevation of posterior cornea surface 1 year after LASIK(r=0.258,P =0.003).Conclusions The cornea posterior elevation increases slightly early stage after LASIK but retains stable level with lapse of time.The refraction diopter before LASIK is the main factor affecting the posterior corneal shape.To reduce the risk of corneal ectasia,more attention should be paid to the reserving of appropriate corneal residual stroma and smallest degree of eccentricity during the ablation procedure.

Adenocarcinoma ; metabolism ; pathology ; surgery ; Aged ; Humans ; Intestinal Neoplasms ; metabolism ; pathology ; surgery ; Intestine, Small ; Ki-1 Antigen ; metabolism ; Lymphoma, Large B-Cell, Diffuse ; metabolism ; pathology ; surgery ; Male ; Neoplasms, Multiple Primary ; metabolism ; pathology ; surgery ; Rectal Neoplasms ; metabolism ; pathology ; surgery

Animals ; Chromosome Deletion ; Chromosomes, Human, Pair 13 ; Genetic Therapy ; Humans ; Liposarcoma ; genetics ; metabolism ; therapy ; Proto-Oncogene Proteins c-mdm2 ; metabolism ; Retinoblastoma Protein ; metabolism ; Soft Tissue Neoplasms ; genetics ; metabolism ; therapy

Objective To study the effect of psychological counseling for patients with thoracic surgery recovery period.Methods Ono hundred patients with lobectomy were selected,they were divided into experimental group and control group with 50 cases each according to the treatment method.Two groups of patients were in the ward completed indwelling catheter,inserted into the right double lumen endotracheal tube by high seniority anesthesiologists,postoperative given perfect analgesia.Two group patients were treated with routine operation before the visit,assess the condition and signed the anesthesia informed consent,the test group were given psychological support therapy,explain in detail postoperative recovery period may be uncomfortable,eliminate the anxiety of patients,establish the confidence to overcome the disease,Two groups of patients after surgery by the recovery room nurse to give psychological counseling,complete extubation and record the restlessness,24 h after the patient satisfaction questionnaire.Results In test group patients were able to follow instructions and coordinate nurse smooth tube removal,15 cases of patients need to brake,additional sedatives and dehyed extubation,the difference between the two groups has statistical significance (P ＜ 0.05).The satisfaction questionnaire in test group was significantly higher than that in control group:100％ (50/50) vs.82％ (41/50),there was statistical difference (P ＜ 0.05).Conclusion Psychological counseling can effectively avoid agitation in recovery period of patients with postoperative chest tube removal,smooth.

Adoptive Transfer ; methods ; trends ; Cells ; pathology ; Cells, Cultured ; pathology ; Humans

Objective To evaluate male bulbourethral sling procedure for the treatment of post prostatectomy urinary incontinence. Methods 7 male patients with severe post prostatectomy incontinence underwent the male bulbourethral sling procedure,the patients being completely incontinent before treatment. Results The patients have been followed up for 14～26 months with a mean of 20.Ideal continence has been achieved in 6 and significantly improved in 1. Conclusions Bulbourethral sling procedure is effective for post radical prostatectomy and intrinsic sphincter deficiency urinary incontinence.

Objective To investigate the effects of DLXNP on hemorrheology and bulbar conjunctiva microcirculation (BCM) in rabbit and to explore its effects of activating the blood and eliminating the stagnant. Methods The blood stagnant syndrome model was reproduced by injecting dextran 500 into the marginal ear vein. The BCM and the hemorrheology properties were detected and compared. Results Compared with the normal group, the model group's indexes of whole blood relative and whole blood reducing viscosity improved (P

Objective To probe the effects of Danlongxingnaopian on hemorrheology and bulbar conjunctiva microcirculation(BCM)in dog and to explore its effects of activating the blood and eliminating the stagnant.Methods The blood stagnant syndrome model was reproduced by injecting dextran 500 into the vein.The BCM and the hemorrheology properties were detected and compared.Results Compared with the normal group,indexes of the model group such as whole blood relative viscosity,whole blood reducing viscosity and plasm viscosity improved,erythrocyte sedimentation rate and erythrocyte sedimentation rate K increased,erythrocyte aggregation index and erythrocyte transmutation index increased,BCM's morphological mark and flow habitus mark increased(P