Objective To study the expression of Ephrin‐B4 receptor (EphB4) in hepatocellular carcinoma (HCC) and its clinical significance ,and to analyze the effect of EphB4 on the proliferation of HCC cells .Methods The expression level of EphB4 in HCC tissues and matched paracancerous liver tissues of 60 cases of HCC patients was assessed by reverse transcriptase‐polymer‐ase chain reaction (RT‐PCR) and immunohistochemistry .The correlation between the expression of EphB4 in HCC tissues and clin‐ical pathologic parameters was analyzed by chi‐square test .Univariate survival analysis was carried out by Kaplan‐Meier Log‐rank test .The effect of EphB4 on the viability of HCC cells was furtherly analyzed by MTS .Results The results of RT‐PCR showed that the mRNA level of EphB4 in HCC tissues (1 .39 ± 0 .80) was significantly higher than in matched paracancerous liver tissues (0 .56 ± 0 .33) ,the difference was statistically significant (P< 0 .01) .Data from immunohistochemistry showed that the positive rates of EphB4 protein in HCC tissues and matched paracancerous liver tissues were 81 .7% and 23 .3% ,respectively .Moreover ,the expression of EphB4 in HCC tissues was relevant to AFP level ,tumor size and TNM stage(P<0 .05) .The three‐year survival rate of HCC patients with positive expression of EphB4 protein was 22 .5% ,and that of HCC patients with negative expression of EphB4 protein was 54 .5% ,the difference was statistically significant (P<0 .05) .Overexpression of EphB4 significantly enhanced the ability of hepatocellular carcinoma cell proliferation (P<0 .05) .Conclusion EphB4 expression was significantly up‐regulated in HCC ,which was associated with HCC progression and prognosis ,and EphB4 could promote the proliferation of HCC cells ,which could be used as a marker of HCC progression .

OBJECTIVE To acknowledge the important factor of bacterial infection of patients after tracheotomy. METHODS Thirty five patients′ lower respiratory tract sputa were sent to conduct bacterial culturing and determine their susceptibility to antibiotics and analyze results with statistics methods. RESULTS Total 8 main kinds of bacteria were isolated and drug resistance rate of Gram-positive cocci over 50 percent was erythromycin,that of(Gram-negative) bacilli included ampicillin,gentamicin,ciprofloxacin,and cefuroxime.The average time of antibiotics usage among 35 patients was(27?19.17) days and 12 kinds of antibiotics were used. CONCLUSIONS The time of usage of different antibiotics is an important factor to aggravate patients′ infection after tracheotomy.

Adenocarcinoma ; diagnosis ; therapy ; Guidelines as Topic ; Humans ; Thyroid Neoplasms ; diagnosis ; therapy

Adenocarcinoma, Clear Cell ; diagnosis ; metabolism ; pathology ; Adenocarcinoma, Mucinous ; diagnosis ; metabolism ; pathology ; Biomarkers, Tumor ; metabolism ; Carcinoma, Endometrioid ; diagnosis ; metabolism ; pathology ; Cystadenocarcinoma, Serous ; diagnosis ; metabolism ; pathology ; Diagnosis, Differential ; Endometrial Neoplasms ; diagnosis ; metabolism ; pathology ; Female ; Humans ; Immunohistochemistry ; Uterine Cervical Neoplasms ; diagnosis ; metabolism ; pathology

Objective To study the influence of combined anti-CD-40L and low dose CsA immunotherapy on the induction of immune tolerance in orthotopic liver transplantation rats.Methods Recipients were divided into the following groups.Group A,SD→SD;Group B,SD→Wistar without any treatment;Group C,SD→Wistar with CsA therapy from d1 to d5;Group D,SD→Wistar with CsA from d1 to d5 and anti-CD40L mAb in d0 and d2,and Group E,SD→Wistar with CsA from d1 to d5 and anti-CD40L mAb in d0 and d2 plus autotransfusion before operation.The recipient survival time,histologic changes of allograft and the level of cytokines in peripheral blood were observed.Results The survival period of recipients in Groups A,D and E was significantly longer than that in Groups B and C.The acute rejection response in Groups D and E decreased prominently.The serum level of IL-2 and IFN-? in Group B were significantly higher than those in other groups,while the serum level of IL-4 and IL-10 in Groups D and E was elevated much more than those in Group B(P

Objective To evaluate the role of MRA,IPVG and DUS in the hemodynamics studies of portal hypertension.Methods Thirty-eight patients with portal hypertension were examined with Philips Gyroscan 1.0 Tesla MR imaging system.3D-DCE MRA and 2D-PC MR were used for study of portal venous anatomy and its hemodynamics.The results were compared with those obtained from IPVG and DUS.Results 3D-DCE MRA could clearly display the anatomical imaging of portal venous system and its imaging quality was better than that of IPVG.The data of hemodynamics from 2D-PC MR including diameter,blood velocity and blood flow were closely correlated to those from DUS.Conclusion As a non-invasive technique,MRA can display the anatomy of portal venous system and measure its hemodynamics exactly.It should be applied as the first choice in hemodynamics evaluation for portal hypertension.

Objective:To study the dynamic frictional resistance in the buccal segment between preadjusted brackets coupled with different archwires,at different times under artificial saliva condition.Methods: The dynamic friction of different combinations of 4 kinds of preadjusted brackets and 4 archwires were tested simulating archwires sliding in the buccal segments at 4 different times(0,15,30,45 d)under artificial saliva condition by using Instron universal testing machine.Results: The dynamic frictional resistance of combinations of 3M MBT bracket and all archwires were the smallest.The combinations of 0.46 mm inch stainless steel round wire and all brackets produced significantly lower dynamic frictional resistance than that of other archwires.The 0.48 mm?0.64 mm inch stainless steel rectangular wire produced the highest dynamic frictional resistance combined with all preadjusted brackets.The dynamic frictional resistance of combinations of archwires and brackets were highest at the 15th day and smallest at the 30th day under artificial saliva condition.Conclusion: 0.46 mm stainless steel round wire produces the smallest dynamic frictional resistance combined with all preadjusted brackets.3M MBT brackets are more favorable to sliding mechanics compared with other brackets.As for time,the lowest dynamic frictional resistance exhibits at the 30th day under artificial saliva condition.

To enhance the fusion of graft bone in thoracolumbar vertebrae and minimize the postoperative loss of correction, short-segment pedicle screw fixation was reinforced with posterior moselizee bone grafting in vertebrae for spinal fusion in patients with thoracrolumbar vertebrate fractures. Seventy patients with thoracrolumbar vertebrate fractures were treated by short-segment pedicle screw fixation and were randomly divided into two groups. Fractures in group A (n=20) were reinforced with posterior morselized bone grafting in vertebrae for spinal fusion, while patients group B (n=50) did not receive the morselized bone grafting for bone fusion. The two groups were compared in terms of kyphotic deformity, anterior vertebral height, instrument failure and neurological functions after the treatment. Frankel grading system was used for the evaluation of neurological evaluation and Denis scoring scale was employed for pain assessment. The results showed that the kyphosis correction was achieved in both group A and group B (group A: 6.4 degree; group B: 5.4 degree)/ At the end of follow-up, kyphosis correction was maintained in group A but lost in group B (P=0.0001). Postoperatively, greater anterior height was achieved in group A than in group B (P<0.01). During follow-up study, anterior vertebral height was maintained only in Group A (P<0.001). Both group A and group B showed good Denis pain scores (P1 and P2) but group A outdid group B in terms of control of severe and constant pain (P4 and P5). By Frankel criteria, the changes in neurological functions in group A was better than those of group B (P<0.001). It is concluded that reinforcement of short-segment pedicle fixation with morselized bone grafting for the treatment of patients with thoracolumbar vertebrae fracture could achieve and maintain kyphosis correction, and it may also increase and maintain anterior vertebral height. Morselized bone grafting in vertebrae offers immediate spinal stability in patients with thoracolumbar vertebrate fractures, decreases the instrument failure and provides better postoperative pain control than without the morselized bone grafting.
Bone Screws ; Bone Transplantation/*instrumentation ; Bone Transplantation/*methods ; Kyphosis/etiology ; Kyphosis/*surgery ; Lumbar Vertebrae/pathology ; Lumbar Vertebrae/surgery ; Nervous System Diseases/etiology ; Postoperative Complications ; Spinal Fractures/*surgery ; Spinal Fusion

OBJECTIVE To survey the current situation of disinfection and supply rooms in 34 hospitals of Hubei Province under the Committee of Hubei Distinfection.METHODS On the basis of certain references,an questionaire was designed to survey on site.RESULTS All 34 hospitals were taken disperse control mode of disinfection;the education was not good enough;most of the disinfection and supply rooms covered limited space,without meeting the standards of Ministry of Health.The rate of equipement usage was low;the working spheres were narrow.CONCLUSIONS There is not any control mode of disinfection and supply rooms in these 34 hospitals;if working staff and equipment are improved,the working spheres can be broaden.it should be transferred to the centralized control mode of disinfection.

Protocarcinogenic gene Her-2/neu was closely related to the development of gastric cancer. Herceptin is an antibody according to Her-2/neu and is used for treating breast cancer. Topo Ⅱ α is the important Nuclear enzymes needed by DNA duplicate, and is closely related to the proliferation of malignant tumors, so it is the important target enzymes of cancer chemotherapy. Her-2/neu and Topo Ⅱ α are located in the same chromosomes, there are correlation between the two genes' proliferation or loss. We reviewed the expression of Her-2/neu and Topo Ⅱ α in the stomach and the new progress of the correlation between the two genes home and abroad in this paper, which provides new methods for the prevention and treatment of gastric cancer.