1.Analysis of current situation of radiotherapeutic equipments used in PLA
China Medical Equipment 2009;(8):34-36
Medical electron linear accelerator and Gamma-ray stereotactic radiotherapy system,two kinds of large scale medical equipment,both have an medical treatment on patient by imposing a high-level of radiation.The accuracy of deferent dose and validity of therapy programe play a very important role to patients'security and treatment effect.Both the military and local governments Pay a lot of attention to the two kinds'allocation and quality control.As technical administrative department.the Medical Equipment Application Quality Test and Research Center of PLA analyse current situation of the two kinds detailedly and point out the existing problems based on quality of the two kinds of equipment used in military medical agencys.
2.Effect of exogenous transforming growth factor-beta 2(TGF-?_2) and bone morphogenetic protein 2(BMP_2) and allografts bone on radius defects healing of experimental rabbits
Orthopedic Journal of China 2006;0(09):-
[Objective]To investigate the effect of exogenous TGF-?2and BMP2 and allografts bone on radius defects.[Method]The model of adult rabbit radius defects was made and treated with TGF-?2 and BMP2 respectively or in combination and allografts bone in the radius defects.A group:BMP2 and allografts bone.B group:autogenous bone.C group:TGF-?2 and allografts bone.D group:BMP2 and TGF-?2 and allografts bone.Fracture healing was evaluated in different time by radiograph,biomechanical tests,measurement of the bone mineral density and calcium content in callus.[Result]The results of B group excelledthose of A,C,D group in aspects of calcium content in callus,the ability of radius defects and biomechanical strength of bone(P
3.Survey of Infective Disease in Community
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To investigate the status of community health services and offer a scientific basis for making a direction of community to control the infective disease.METHODS Totally 1503 outpatients were investigated retrospectively for infective diseases in community.RESULTS The average age of these patients was 65,the number of females was much more than males.The most frequently infective diseases were upper respiratory tract infection,bronchitis,periodontitis,etc.CONCLUSIONS It′s necessary to strengthen the community health service and to establish an effective system of health service.The patients must cultivate a health habit of life,they not only need in time diagnosis and treatment,but also enhance own knowledge about health and disease prevention.
6.Eradication of Haemophilus influenzae in Biofilms in vitro by Moxifloxacin
Dong WANG ; Ying WANG ; Youning LIU
Chinese Journal of Nosocomiology 2006;0(07):-
OBJECTIVE To determine the activity of moxifloxacin on bacterial biofilms formed by Haemophilus influenzae in vitro.METHODS Formation of bacterial biofilm was examined by crystal violet assay,viable cells counting in biofilms was also carried out.Alterations of biofilms were measured under varying concentrations of moxifloxacin.RESULTS Optical density values of biofilms were significantly decreased at the concentrations higher than 4 mg/L of moxifloxacin.The similar results were obtained for viable cell counting.Bacteria in biofilms were eliminated partly or completely at concentrations higher than 0.25 mg/L.CONCLUSIONS Moxifloxacin is able to destroy the biofilms and reduce viable cells counting at high concentrations.
7.Simultaneous Determination of Main Components in Compound Telmisartan Tablets by HPLC
Yanming LIU ; Xuefang WANG ; Dong WANG
China Pharmacist 2015;18(12):2171-2172,2179
Objective:To establish an HPLC method for the simultaneous determination of telmisartan and amlodipine besylate in tablets. Methods:The isocratic separation was achieved on a Phenomenex C18 column (150 mm × 4. 6 mm, 5 μm) with the mobile phase composed of 50 mmol·L-1 sodium dihydrogen phosphate buffer (pH 6. 0, 0. 5% triethylamine)-acetonitrile (40:60, v/v). The flow rate was 1. 0 ml·min-1 , the detection wavelength was 254 nm, the column temperature was 30℃ and the sample size was 20μl. Results:Telmisartan could be well separated from amlodipine besylate under the conditions mentioned above. The linearity be-tween the peak area and the concentration was obtained within the range of 4. 0-80. 0 μg·ml-1(r=0. 999 9) for telmisartan and 1. 0-20. 0 μg·ml-1(r=0. 999 9) for amlodipine besylate. The mean recovery of telmisartan and amlodipine besylate was 99. 90% and 100. 52%, and RSD was 0. 74% and 1. 48%, respectively (n=9). Conclusion:The method is specific, stable and accurate in the determination of compound telmisartan tablets.
8.Laparoscopy combined with esophagogastroscopy for achalasia of the cardia:Report of 25 cases
Long LIU ; Qiusheng WANG ; Lei DONG
Chinese Journal of Minimally Invasive Surgery 2005;0(08):-
Objective To investigate the value of laparoscopy combined with esophagogastroscopy in the treatment of achalasia of the cardia. Methods Twenty-five patients with achalasia of the cardia between October 2003 and April 2006 in this department underwent the HellerDor operation under laparoscopy combined with esophagogastroscopy.Results A1l the operations were successfully accomplished.The operating time was 60~240 min(mean,107 min) and the intraoperative blood loss,5~60 ml(mean,22 ml).Intraoperative mucosal perforation was encountered in 4 patients.All the patients got out of bed for activities at 8 hours after operation,with first passage of flatus,removal of the gastric tube,and liquid diet intake at 1~3 days after operation(mean,1.5 days).The esophageal manometry showed the lower esophageal sphincter(LES) pressure had decreased to normal levels at 7 days after operation.The 24-hour ambulatory pH studies showed a high reflex index in 3 patients,but without symptoms.Upper gastrointestinal barium swallow showed the dilated esophagus had a 8~26 mm(mean,18 mm) decrease in diameter at 8 days after operation.The length of postoperative hospitalization was 8~11 days(mean,8.5 days).Follow-up for 1~30 months(mean,5.4 months) in all the patients showed 23 patients were asymptomatic and 2 had intermittent dysphagia.Conclusions Heller-Dor operation under laparoscopy combined with esophagogastroscopy has advantages of precise positioning,little invasion,less pain,and good effects,increasing the quality of operation as well as decreasing the incidence of complications.
9.Clinical application of domestic harmonic scalpel in laparoscopic cholecystectomy
Lei DONG ; Qiusheng WANG ; Long LIU
Chinese Journal of Minimally Invasive Surgery 2005;0(12):-
Objective To explore the clinical value of domestic harmonic scalpel in laparoscopic cholecystectomy. Methods Modified laparoscopic cholecystectomy (LC) was performed in 36 cases under general anesthesia. The operation was completed by using domestic USI harmonic scalpel in 21 cases (USI Group) and by using imported harmonic scalpel in 15 cases (GEN Group). Results The operation was laparoscopically accomplished in both of the groups, without conversions to open surgery. Of the USI Group, the operating time was 42.6?7.5 min, the postoperative drainage was 34.3?14.0 ml, the length of postoperative hospitalization was 4.9?0.9 d, the speed for cutting cystic duct was 335.1?180.3 ?m/s, the speed for cutting cystic artery was 524.2?127.8 ?m/s, and the denaturalization width of cystic duct was 2 047.6?376.3 ?m. Of the GEN Group, the operating time was 47.0?9.0 min, the postoperative drainage was 38.0?3.9 ml, the length of postoperative hospitalization was 4.3?0.9 d, the speed for cutting cystic duct was 403.5?120.1 ?m/s, the speed for cutting cystic artery was 513.5?125.9 ?m/s, and the denaturalization width of cystic duct was 2 266.7?306.3 ?m. There was no differences in these parameters between the two groups ( P
10.Differentiation of PC12 cells into neurons induced by NGF
Xiuli WANG ; Dong CHEN ; Jiamei LIU
Journal of Jilin University(Medicine Edition) 2006;0(05):-
Objective To study the condition of pheochromocytoma cells(PC12 cells)differentiation into neurons induced by nerve growth factor(NGF).Methods There were five groups :10,20,50,and 80 ?g?L-1 NGF groups and control group which contained 10% fetal calf serum.PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h,respectively.The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h.Results The number of MAP2 positive cells was significantly increased in 20,50,and 80 ?g?L-1 groups compared with control group and the most significant concentration was 50 ?g?L-1(P