Objective To explore the difference of hand hygiene requirement between guide-line of skill practice for national examinations of doctors' qualification(clinical practitioners) and stan-dard for hand hygiene for healthcare workers in health care settings for some sterile techniques. Methods Eight sterile techniques in guideline of skill practice for national examinations of doctors' qualification(clinical practitioners) such as changing a dress and taking out stitches, aspiration, ure-thral catheterization, arteriovenous puncture, thoracentesis, abdominal puncture, lumbar puncture and bone marrow puncture were chosen and the results were compared with standard for hand hygiene for healthcare workers in health care settings. Results Three of eight sterile technics mentioned hand-washing, six of eight mentioned wearing sterile gloves, and zero of eight mentioned antiseptic han-drubbing; however, only one of six (16.67%) before wearing sterile gloves mentioned handwashing. Conclusion The requests of hand hygiene between guideline of skill practice for national examina-tions of doctors' qualification (clinical practitioners) and standard for hand hygiene for healthcare workers in healthcare settings are not concordant , which from the root leads to the inconsistence of doctors' learning at different stages of education and training . It is suggested that the examination organization departments should refer to the relevant provisions of the state in the preparation of guidelines, and regulate medical practice behavior with consistent standards.

Objective To re-evaluate Danhong injection and analyze the rationality of medication. Methods 400 patients treated with Danhong injection in Taiyuan Centre Hospital from February to August, 2010 were monitored centralizedly.Their medication and the results were analyzed and evaluated. Results The improvement rate was 95.1%, and the incidence of adverse reaction was 0.5%. The problems showed in the use of the drug were non-indication medication,inappropriate selection of solvent, and unsuitable compatibility with other drugs. Conclusion Danhong injection has the advantages of perfect effects and safe. The mode of medication monitoring by clinical pharmacist is worth promoting.

Objective To understand the effect of environment variables on anxiety in the soldiers at high altitude.Methods A total of 267 soldiers at high altitude were investigated with the self-rating anxiety scale,the environmental scale and Eysenck personality questionnaire.Results Compared with norm(29.78±0.46),the soldiers at high altitude had higher sore of anxiety(37.22±6.64;t=17.92,P＜0.01).One-way ANOVA and independent.samples t test showed that the anxiety(38.37±5.93,36.65±6.951,36.75±6.88)of military people at high altitude had not evidently difference in different altitude(≥3587.5m,3587.5～2767.9m,≤2767.9m).Parental culture.length of military service(F=3.25,P＜0.05)and neuroticism(R=0.52,P＜0.01)had evidently effect on the depression of soldiers.Conclusion The occurrence of anxiety in the soldiers at high altitude has a higher level.Parental culture.1ength of military service and neuroticism had predictive value for depression.

The view of "Harmony between Man and Nature" in Song-Ming period,taking "the same rule of man and nature" as mode,"clearing the rules according examining the things" as means,made the doctors of vital gate school in traditional Chinese medicine set up a life pattern of "taiji(vital gate)-yin and yang-five elements(zang-fu viscera)",so as to explore the origin of life and mystery.The study on the characteristics of view of "Unity of Heaven and Man" in different historical stages is conducive to grasp the track and skeleton of the formation of traditional Chinese medical theories,lay the foundation for the further development of traditional Chinese medicine.

Objective To construct,express,purify,identify and label the TAT-Smad7-HA fusion protein (protein transduction domain of trans-activator,human Smad7 and hyaluronic acid) and to validate its transduction activity in the cultured human primary keloid fibroblast cells. Methods TAT-PTD,Smad7 and HA fragments were sequentially inserted into pET32a(+) to construct the pTAT-Smad7-HA prokaryotic expression vector. After the expression of target fusion protein was induced to express by IPTG,affinity purification,Western blot analysis,enterokinase cleavage,target protein capture and FITC labeling were subsequently performed by turns to obtain the FITC-TAT-Smad7-HA fusion protein and to further observe its transduction activity in the human primary KFB cells in vitro. Results The prokaryotic expression vector for the TAT-Smad7-HA fusion protein,named as pTAT-Smad7-HA was successfully constructed,and the target fusion protein was efficiently induced to express,covering more than 25% of the total bacterial proteins,successfully purified with a purity of more than 95% purity,desalted by desalting column,identified by Western blotting,thioredoxin removed and FITC labeled. Finally,a fusion protein of FITC-TAT-Smad7-HA with the approximately molecular weight of 50?103 was successfully purified and its high transduction activity in KFB cells was validated. Conclusion The highly-purified FITC-TAT-Smad7-HA fusion protein and the validation of its high transduction activity in KFB cells have provided an experimental foundation for further studies on the role the human Smad7 protein playing in the TGF-?/Smads signal transduction pathway and further elucidation of the pathogenesis of keloid formation.

Cathepsin S is one of the cathepsins that distributes generally at lysosomes of histiocyte in humans body.It has variety of actions when it is unbalancing,including promote inflammation,atherosclerosis,diabetes and obesity,etc.Studies in recent years have demonstrated that there are certain correlations of adiponectin with vascular risk factor and cerebrovascular disease.

Audiometry, Speech ; Humans ; Language

Parkinson's disease (PD) is a common neurodegenerative disorder associated with aging. Great progresses have been made toward understanding the pathogenesis over the past decades. It seems that both genetic factors and environmental factors contribute to PD, while the precise pathogenesis still remains unknown. Recently, increasing evidence has suggested that autophagy dysregulation is closely related to PD. Dysregulation of the autophagic pathways has been observed in the brains of PD patients or in animal models of PD, and a number of PD-associated proteins, such as a-synuclein, Parkin and PINK1, were found to involve in autophagy, suggesting a link between autophagy and pathogenesis of PD. In this review, we summarized the role of PD-associated proteins in autophagy pathways. In addition, we described the efficacy of autophagy-modulating compounds in PD models and discussed promising strategies for PD therapy.
Animals ; Autophagy ; Humans ; Parkinson Disease ; physiopathology ; Protein Kinases ; metabolism ; Ubiquitin-Protein Ligases ; metabolism ; alpha-Synuclein ; metabolism

Objective To evaluate the clinical value of multi-slice spiral CT in diagnosing tracheobronchial foreign bodies in chil-dren.Methods 139 patients with airway foreign body confirmed by bronchoscopy were examined with 128-slice spiral CT,and then the CT data was reconstructed by using multiple-planar reconstruction(MPR),curved planar reconstruction (CPR),volume render-ing(VR),minimum intensity projection(MinIP)and virtual endoscopy (VE).The CT results were compared with bronchofibersco-py findings.Results Among the 139 cases,the foreign bodies located at trachea in 13 cases,right bronchia in 75 cases,left bronchia in 48 cases,and bilateral bronchia in 3 cases.137 cases (98.6%)were correctly diagnosed,and 2 cases (1.4%)were missed on CT.Although the difference of the results at trachea and right main stem bronchus was statistically significant (P <0.05),the over-all results was not significant difference between CT and bronchoscopy examination (P =0.340).Associated findings included em-physema in 74 cases,pneumonia in 48 cases,atelectasis in 22 cases,mediastinal displacement in 57 cases,lung interstitial emphyse-ma in 4 cases,pneumomediastinum and pneumoderma in 4 cases,bronchiectasis in 2 cases,and pleural effusion in 4 cases.Conclu-sion MSCT and its postprocessing techniques can accurately and intuitively display the tracheobronchial foreign body and complica-tions,which has important value for clinical application.

[Objective]To evaluate the feasibility of early off-bed exercises after arthroscopically assisted radiofrequency release of gluteal muscle contracture.[Method]Patients of gluteal muscle contracture underwent arthroscopically assisted radiofrequency release of gluteal muscle contracture.Post-operative off-bed time,side effects,duration of urinary catheter drainage,wound healing,early recovery of function,and in-hospital time were studied.[Result]Off-bed time averaged 7 hours(6~8 hours).Two cases complained of being faint and nausea.After allopathic treatment,no serious complications appeared.Averaged duration of urinary catheter drainage was 7.5 hours.Primary healing of wounds were found in 67 patients,while 1 case got delayed wound healing.None of them had any infections.Functional scores averaged 9 at the time of discharging and 10 after 1 month.Post-operative in-hospital time averaged 2.58 days(2~6 days).[Conclusion]Arthroscopically assisted radiofrequency release of gluteal muscle contracture results in early post-operative off-bed time and reduction of suffering,which is propitious to functional recovery,rate of healing,and shorter in-hospital time.