Objective To explore the effect of antidepressant adjuvant treatment for chronic prostatitis (CP),and to find an effective treatment for the clinical therapy of CP.Methods 88 patients with CP were randomly divided into two groups,the control group 41 patients were treated with the antibacterials moxifloxacin,the treatment group 47 patients were treated with the moxiiloxacin treatment and paroxetine adjuvant therapy.The clinical symptoms,life quality (QOL) and Zung depression scale score of the two groups were evaluated and compared.Results After four weeks of treatment,voiding difficulty endless,urinary urgency,perineal discomfort symptoms of the treatment group were improved significantly compared with the control group (x2 =23.563,18.348,15.874,all P ＜ 0.05).After four weeks of treatment,QOL,Zung depression scale scores the treatment group were more significant decline than before treatment and the control group after treatment (t =0.554,0.615,0.382,0.336,all P ＜ 0.05).Conclusion Antidepressant adjuvant therapy improves the psychiatric symptoms of CP patients,which can effectively improve the clinical efficacy of antibiotic therapy of CP.

Objective To discuss the pathogenesis and clinical characteristics of acute relapsing pancreatitis .Methods The clini-cal data and relevant data of 274 acute pancreatitis cases from January 2010 to and December 2012 were analyzed ,of which one group of 231 cases were first onset and the other group of 43 cases were relapsing .Results The probability of relapse of acute pan-creatitis were highest from 1 month to half a year after first onset (20 cases ,accounting for 46 .51% );biliary factors were the major causes of first onset and relapse cases ,where the relapsing group was higher than first onset group ,with statistical significance (P<0 .05);the relapsing group had more severe symptoms of hyperlipidemia than the first onset group ,with significant difference (P<0 .05) ,The relative risk (OR value) of relevant factors were higher than biliary factors ;improper diet ,alcohol and other factors of the relapsing group were lower than the first onset group ,without statistical significance (P> 0 .05);there was no significant difference between the occurrence rate of severe pancreatitis and the occurrence rate of surgeries and deaths .Conclusion Biliary diseases and hyperlipidemia are closely related with the relapse of acute pancreatitis .

Objective To explore the reversion of astragaloside and NMDA receptor antagonist against the hyperphosphorylation of protein Tau induced by fetal intrauterine distress in neonatal rats.Methods The analysis of variance of factorial design was setted up two intervention factors which were fetal intrauterine distress (two levels:no disposition;a course of fetal intrauterine distress)and the drugs (three levels:iv Saline;iv astragaloside;iv MK -801).When the neonatal rats grew to 12weeks,the hippocampus was removed from the neonatal rats.Detected the content of glutamate in the hippocampus of rats by high performance liquid chromatography.The expression of protein Tau which includes p -AT8Ser202 and GSK -3β1H8 in the hippocampus of rats were detected by the methods of immunohistochemistry staining.Results Fetal intrauterine distress could significantly up -regulate the content of glutamate,which was not affected by MK -801,in the hippocampus of neonatal rats which was reduces by the astragaloside (P <0.05).And both influences presented subtracting effects (P <0.05).Fetal intrauterine distress and the drugs do not affect the total protein Tau in the hippocampus of rats (P <0.05).Fetal intrauterine distress could up -regulate the hyperphosphorylation of protein Tau in the hippocampus of neonatal rats which could be reduced by astragaloside and MK -801 (P <0.05).And the influences between fetal intrauterine distress and the drugs presented subtracting effects (P <0.05 ).Conclusion Our results indicate that fetal intrauterine distress reduce the hyperphosphorylation of protein Tau in neonatal rats though up -regulating the content of glutamate.GSK-3βis the key protein in this signaling pathway.

Objective To explore protective effects and potential underlying mechanisms of panax notoginseng (PTS) on retinal ganglion cell (RGC) in diabetic rats. Methods SD rats were randomly divided into control group, diabetic group and treatment group. The diabetic model rats were induced by intraperitoneal injection of streptozotocin. Rats were giv-en PTS 50 mg·kg-1·d-1 in treatment group. One month later, the coexistence of nogo receptor and Brn3a (special marker of RGC) was observed by immunofluorescence staining double-labeled method. The expression of Nogo receptor was detected by Western blot assay. The level of malondialdehyde (MDA) in retina was measured with detection kit. HE-staining was in-troduced to reveal the number of retinal RGC. Results A large number of Brn3a and Nogo receptors were co-existed in the retina. The Nogo receptor was exclusively expressed in RGC, which was up regulated in diabetic group compared with that of control group. The level of retinal MDA was increased and the number of RGC decreased in diabetic group than that of con-trol group (P＜0.001). Compared with diabetic group, there were decreased retina Nogo receptor, decreased level of MDA and increased number of RGC in treatment group (P＜0.001). Conclusion PTS attenuates diabetes-induced loss of RGC, which may ascribe for down-regulation of retina Nogo receptor and decreased oxidative stress.

Objective To discuss the application of early intraperitoneal perfusion combined with intravenous chemotherapy in patients with advanced colorectal cancer.Methods 100 patients with advanced colorectal cancer were randomly divided into two groups, 50 cases in each group.The control group was treated with intravenous chemotherapy alone after surgery.The observation group was given low perfusion combined with intravenous chemotherapy after surgery.The clinical effect and adverse reaction of the two groups were compared.Results The total effective rate and 3-year survival rate of the observation group(86.0%,34.0%) were significantly higher than those of the control group(68.0%,20.0%).The recurrence rate and metastasis rate of the observation group(14.0%,10.0%) were significantly lower than those of the control group(34.0%,28.0%), the differences were statistically significant(x2=4.482,4.486,5.482,5.263,all P<0.05).At the same time,there was no significant difference in the incidence of adverse reactions between the two groups(P>0.05).Conclusion Early intraperitoneal perfusion combined with intravenous chemotherapy in the treatment of advanced colorectal cancer can improve the long term survival rate of patients,and the curative effect is accurate.Therefore,it has the value of popularization and application in clinic.

OBJECTIVE:To study anti-arrhythmic effects of Lianshen lyuping dripping pills in rats,mice,guinea pigs. METH-ODS:48 SD rats were randomized into a normal control (isometric normal saline) group,a model (isometric normal saline) group,a propafenone (positive control,270 mg/kg) group and Lianshen lyuping dripping pills high-dose,medium-dose and low-dose(210,140,70 mg/kg)groups. The rats’tails were given the drugs iv at a time,10 min later,aconitine(10 mg/L,3 μg/min) iv. The doses of aconitine were recorded when ventricular premature beat(VP),ventricular tachycardia(VT)and ventricular fibril-lation(VF)developed in ECG. 48 guinea pigs were grouped and given drugs as above and 10 min later,were given uabain iv(1 mg/L,1 μg/min). The doses of uabain were recorded when VP,VT and VF developed in ECG. 120 KM mice were grouped and given drugs ip at a dose as above at a time,30 min later,were placed in the beaker containing 4 ml chloroform. When respiratory arrest occurred,ventricular fibrillation rate of mice were calculate.120 Wistar rats were grouped and given drugs at a dose as above,following typical arrhythmia reaction,given 0.8% BaCl2 (0.25 ml/kg) iv and sublingually at a time;48 Wistar rats were grouped and given drugs at a dose as above and,following typical arrhythmia reaction, given 5%CaCl2(0.28 ml/kg)iv and sublin-gually at a time. Recovery of sinus arrhythmia rate of rats were determined.48 SD rats were grouped and given drugs at a dose as above,after the administration ig once daily for 7 consecutive days,the rat models of myocardial ischemia reperfusion were estab-lished,and then VT,VF incidence rate and death were recorded. RESULTS:Compared to the normal control group,those in the model group were all abnormal in all indexes. Compared to the model group,those in Lianshen lyuping dripping pills high-dose, medium-dose and low-dose groups used more aconitine and uabain at the time of the occurrence of VP,VT and VF,had a lower occurrence rate of VF in mice and higher recovery of sinus arrhythmia rate in rats . The rats in Lianshen lyuping dripping pills high-dose and medium-dose groups which had myocardial ischemia reperfusion demonstrated a lower of VT,VF incidence rate and death rate. There were statistically significances (P<0.01 or P<0.05).CONCLUSIONS:Lianshen lyuping dripping pills have an anti-arrhythmic effects in animals to some degree.

Objective To investigate the clinical effects of four different bowel preparation methods for colonoscopy.Methods 620 patients examined by coloscopy were randomized into four groups (group A,B,C and D),with each group in 155 cases.Group A received magnesium sulfate.Group B received compound polyethylene glycol.Group C received magnesium sulfate combined with simethicone.Group D received compound polyethylene glycol combined with simethicone.The quality and influencing factors,the detection of polyps and adverse events were compared between the four groups.Results The good bowel cleaning rate of group A,B,C and D were respectively 84.52％,91.61％,85.81％ and 94.19％.The good bowel cleaning rates of group A and C were higher than those of group B and D (x2 =4.32,P ＜ 0.05;x2 =7.63,P ＜ 0.05;x2 =4.10,P ＜ 0.05;x2 =6.06,P ＜ 0.05.).The bubble free rate of group A,B,C and D were 86.45 ％,87.74％,92.26％ and 90.96％.The bubble free rates of group C and D were higher than those of group A and B (x2 =4.17,P ＜ 0.05;x2 =4.76,P ＜ 0.05;x2 =5.02,P ＜ 0.05;x2 =4.23,P ＜ 0.05).The polyp detection rates of group A,B,C and D were 35.4％,36.8％,51.6％ and 54.2％.Group C and D showed higher quality of foam eliminating and detection rate of polyps as compared with group A and B (x2 =8.24,P ＜0.05;x2 =11.04,P ＜0.05;x2 =6.95,P ＜0.05;x2 =9.53,P ＜0.05).The incidence of adverse reactions of group A and C (21.3％ and 19.4％) were higher than group B and D (7.8％ and 6.5％) (x2 =11.86,P＜0.05;x2 =14.95,P ＜0.05;x2 =9.18,P ＜0.05;x2 =11.95,P ＜0.05).The frequency of colonoscopy,constipation,medication and examination time interval,bowel preparation methods and adverse events were the the influ ence factors of bowel preparation.Conclusion The quality of bowel preparation of compound polyethylene glycol electrolyte powder is better than magnesium sulfate with high security.Treatment of combination with simethicone can improve bowel preparation quality.Many factors can affect bowel preparation,which need to be paid attention to.

BACKGROUND:Daclizumab can be special y combined with the inerleukin-2 receptor on the surface of activated T cells in human body, and this method can reflect the close of interleukin-2 receptor thus inferring the effect of induction therapy. At present, the daclizumab has been widely used in renal transplantation, but there is no consensus on its clinical application in liver transplantation. OBJECTIVE:To investigate the expression of serum CD25+T cells and soluble interleukin-2 receptor in the patients receiving daclizumab for liver transplantation during perioperative period. METHODS:A total of 58 patients received orthotopic liver transplant for the first time were included and then the patients were randomly divided into two groups:control group (n=28) and treatment group (n=30). The patients in the two groups were treated with tacrolimus, mycophenolate mofetil and corticosteroids triple immunosuppressive regimen. The patients in the treatment group received immune induction therapy with daclizumab, and the patients in the control group did not receive daclizumab. RESULTS AND CONCLUSION:Flow cytometry and enzyme-linked immunosorbent assay showed the expression levels of CD25+T cells in the treatment group were significantly lower than those in the control group at different time points after liver transplantation (P<0.01);and the expression levels of soluble interleukin-2 receptor in the treatment group were lower than those in the control group during transplantation and at the first day after transplantation (P<0.05, P<0.01). At 6 months after transplantation, the incidence of acute rejection was decreased in the treatment group (P<0.01). The results indicate that daclizumab can effectively suppress the expression level of CD25+T cells, as wel as the expression level of soluble interleukin-2 receptor in the peripheral blood in the early stage of liver transplantation, thus effectively reducing the rate of acute rejection.

Objective To investigate the effect of nursing intervention on bowel cleaning of patients with senile constipation during electronic bowel preparation for colonoscopy.Methods Forty patients with senile constipation undergoing routine bowel preparation for colonoscopy were treated with nursing intervention.The bowel cleaning effect and the satisfaction degree after bowel cleaning were assessed. Results The qualification rate of bowel cleaning reached 92.5%,and the excellence rate of cleaning comfort 95.0%.Conclusion The pertinent nursing intervention to the elderly patients with constipation undergoing electronic colonoscopy may improve the rate of cleaning the bowels as well as their level of comfort so that the patients can live smoothly through the electronic colonoscopy.

The health check-up results of 11 514 subjects in 2011 at Beijing Hospital were retrospectively analyzed,including the results of 13C urea breath test for detection of Helicobacter pylori (Hp) and HbA1c test.The results showed that the prevalence rate of Hp infection was significantly higher in females (31.77％) than that of males (29.36％) (x2 =7.355,P ＜0.05) ; the highest prevalence was found in 35-60 y age group (31.17％).Hp positive rates in subjects with body mass index (BMI) ≥28kg/m2 and central obesity were 31.55％ and 30.44％,respectively; there were no significant differences from those with normal weight and non central obesity.Subjects with positive Hp infection had higher levels of HbA1 c,compared to those with negative Hp infection (t =-2.276,P =0.01).In subjects with BMI ≥24 kg/m2 and central obesity,HbA1c level was higher in Hp positive subjects than that in Hp negative ones (P ＜0.05).Diabetes mellitus was positively associated with Hp infection (OR 1.282; 95％ CI 0.941-1.799),particularly in obese patients (OR 1.459 ; 95 ％ CI 1.070-2.071).The results indicate that Hp may be involved in the impairment of glucose regulation,particularly in those with higher body mass index.