Objective To observe the short-term fusion rate of using osteophyte filled cage in ante-rior cervical disectomy and fusion. Methods From May 2007 to May 2009, 103 patients receiving anterior cervical disectomy, spine fusion by different surgeons were performed with cages containing exclusively au-tologous osteophyte collected from both anterior and posterior vertebral edges adjacent to the intervertebral space. There are 69 males, 34 females, from 25 to 77 years of age, average 53.6 years. 60 patients received one level fusion. 33 and 10 patients received two-level and three-level fusion respectively. Patients were re-viewed 3 months after operation and fusion was evaluated by X-ray film, reconstructive CT and MR. Results 1. Only 1.9% (2/103) of patients' X-ray films indicate non-fusion (internal fixation failure) while 31.7% (32/ 103) of patients' CT and 30.1% (31/103) of patients' MR indicate non-fusion or being confusing to make a diagnosis. 2. The diagnostic coherence between CT and MR as to evaluate fusion is good (kappa=0.686, P= 0.00). 3. The total fusion rate was 77.7%(80/103) while the highest fusion rate achieved by single surgeon was 90%(18/20). The total one level fusion rate was 80%(48/60) while the highest rate by single surgeon was 88.2%(15/17). The total two-level fusion rate was 78.6%(26/33) while the highest rate by single sur-geon was 90%(9/10). The total three-level fusion rate was 60%(60/100) while the highest rate by single surgeon was 100%(3/3). No statistic significant difference in fusion rate was found between any two of the three groups. Conclusion The method of using osteophyte filled cage can acquire ideal short-term fusion rate in one and two-level anterior cervical disectomy, fusion and plate fixation.

Objective:To study the relationship betwen the mechanism of autoimmune disease and CD4+CD25+ T cell population in NIK mutated mice-aly mice.Methods:NIK mutated mice-aly/aly mice were used as model,aly/+mice as NIK normal control;cell populations were determined by FACS and the thymus structure were analyzed by immunohistochemistry.Results:The CD4+CD25+CD8- population were remarkably decreased in aly mice;and the UEA-1 positive cells were absent in aly mice.Conclusion:The autoimmune disease in aly mice might be the result of deceased the CD4+CD25+ population;the UEA-1 positive cells might play an important role in the development of CD4+CD25+ population. [

Objective To explore the long-term clinical effect and safety of tacrolimus (FK506) in renal transplant recipients. Methods There were 126 recipients received FK506 based immunosuppressive therapy and 109 recipients received Cyclosporine A (CsA) based immunosuppressive therapy enrolled into our study according to the criterion and were followed up for 3 years. The dose and trough blood concentrations of FK506, incidence of rejection and side-effect, survival of patients and grafts were recorded in detail. Results The 1-, 3-year patients/grafts survival of FK06 group and CsA group were 98.4?% / 96.8?% , 95.2?% / 90.5?% and 97.2?% / 96.3?% , 94.4?% / 89.0?% , respectively. The incidence of acute rejection was 13.5?% and 19.3?% ( P

Objective To improve the capability of diagnosing and treating renal chromophobe cell carcinoma (RCCC) and the recognition of this subtype of renal cell carcinoma (RCC). Methods The clinical data of 15 cases of RCCC (10 men and 5 women) were analyzed.Their age ranged from 47 to 74 years,with a mean of 57 years. Radical nephrectomy was performed in all these cases. Results Postoperative pathological findings confirmed the diagnosis of RCCC.The pathologic TNM stage of RCCC was as follows:pT 1N 0M 0 in 6 cases,pT 2N 0M 0 in 5,pT 3bN 0M 0 in 2,pT 1N 2M 0 in 1 and pT 2N 2M 0 in 1.The pathologic grade of RCCC was G 2 in 10 cases and G 3 in 5.Eleven cases were followed up.During the follow-up of 2 to 31 months (mean, 19 months),1 died of heart attack,1 had local recurrence and 9 were surviving tumor free. Conclusions RCCC is a morphologically distinctive uncommon subtype of RCC.Radical nephrectomy is the first choice for the treatment of RCCC.Compared with other types of RCC at the same stage and of the same grade,RCCC has a better prognosis.

Objective To improve the level of diagnosis and treatment of non-specific ureteritis. Methods The clinical data of 11 cases of non-specific ureteritis (6 men and 5 women) were retrospectively analyzed. Their mean age was 48 years (range,24～67 years).The lesions were detected in upper part of the ureter in 1 case,in middle part in 3,and in lower part in 7.All the lesions were unilateral strictured;of them unifocal strictures were found in 9 cases and multifocal strictures in 2.Open surgery was performed in 7 cases,biopsy and double J stent insertion in 4;of the 4 cases balloon dilation was performed in 1. Results Pathological findings confirmed the diagnosis of non-specific ureteritis.During follow-up of 6 months to 2.4 years (mean,1.6 years) for 10 cases,hydronephrosis was cured in 3 cases,alleviated in 6,unchanged in 1.Of these cases,2 underwent nephroureterectomy.No recurrence and canceration were found in the ureters of all cases. Conclusions Clinically,exploratory operation and biopsy are very important for suspected disorders of ureter before performing nephroureterectomy.

Objective To investigate the clinical features of anuria caused by pediatric congenital bilateral ureteral stenotic obstruction,and to improve the diagnosis and treatment of the disease.Methods This series included 7 infants(2 males and 5 females;age range,35-57 d) with congenital bilateral ureteral stenotic obstruction.The clinical presentations were characterized by sudden anuria or oliguria.B-ultrasound,cystography and puncture pyelography were performed in all of them,and magnetic resonance urography(MRU) in 3.Based on the definite diagnosis,resection of stenotic segment of the ureter or ureteral implantation was performed on them.Results The diagnosis was confirmed by puncture pyelography in all of the 7 cases.Six cases underwent phase I surgery and recovered.No ureteral stenosis and cystoureteral reflux occurred during a follow-up of 2-4 years.The renal function was normalized in them.One case had stenosis recurrence due to scar formation,and was cured by the second operation.Conclusions Anuria caused by pediatric congenital bilateral ureteral stenosis should be diagnosed and treated as early as possible.Definite diagnosis can be established by puncture pyelography and MRU.Resection of the obstructive segment of the ureter,protection of renal function,and ureteral implantation are the main therapies.

The current mental health of college students can not be optimistic.This paper summarized college students' psychological crisis from the perspectives of psychological crisis and crisis intervention,came up with updated research outcomes and deficiencies,and pointed out the direction of its future research and development.

Objective Clearing HIV provirus is the key to cure AIDS .The study was to construct the Tre enzyme eukaryotic expression vector and identify its function in specific recognition of loxLTR sequence in HIV provirus . Methods Tre gene was in-serted into eukaryotic expression vector pcDNA 3.1 gene recombination manipulation by genetic recombination techniques including gene synthesis , PCR, restriction enzyme digestion and ligation .EGFPpA-LoxLTR sequence was inserted into pmCherry-N1 vector and was tested by restriction enzyme digestion , PCR and sequencing .Constructed vectors were electroporated into HeLa cells , then using fluorescence microscopy to observe fluorescence intensity changes . Results PCR, restriction enzyme digestion , electrophoresis and sequencing confirmed that Tre enzyme eukaryotic expression vector had been constructed successfully , and it could specifically recog-nize and cut loxLTR sequence after being transfected into Hela cells . Conclusion Constructed Tre enzyme eukaryotic expression vector can be expressed in Hela cells and specifically recognize loxLTR sequence , which has prepared the experimental ground for fur-ther studies of clearing HIV provirus .

Objective To explore the expression level of calmodulin (CaM) in non-small cell lung carcinoma (NSCLC) and its clinical significance. Methods The expression levels of CaM and CaM mRNA were detected in 64 human NSCLC samples by immunohistochemistry and in situ hybridization, respectively. Results The positive rates of CaM mRNA and CaM protein were 60.4% and 65.6%, respectively. The positive rates of CaM mRNA in low-differentiated samples were significantly higher (70.0%) than those in moderate- to well-differentiated samples (45.3%). The expression rates of CaM in Ⅲ-Ⅳ stages (81.8%) were significantly higher than those inⅠ-Ⅱstages (51.6%) (P

Objective To explore the relationship of the expression levels of laminin (LN) and calmodulin (CaM) in non-small cell lung carcinoma (NSCLC) with its metastasis and prognosis. Methods The expressions of CaM and LN were detected in 64 human NSCLC samples by immunohistochemistry. Results The expressions of LN and CaM were closely associated with the differentiation and the grades of NSCLC as well as the lymph node metastasis. The positive rates of LN in adenocarcinoma and squamous cell carcinoma were 45.1% and 69.7 (P0.05), respectively. The positive rate of LN protein in low-differentiated samples (36.6%) was significantly lower than that in moderate- to well-differentiated samples (61.7%), but the positive rates of CaM were 73.3% in low-differentiated samples and 41.1% in moderate- to well-differentiated samples (P