Objective To study the effect of promoting blood circulation to remove blood stasis by Qiangxin Fumai Granule (QXFMG). Method The rat model with blood stasis was induced by the hyodermic injection of adrenaline and ice water-bathing. The blood sample was collected by the puncture of the abdomen aorta and the effect of QXFMG on hemorheological parameters was observed. Result Comparing with the control groups, the QXFMG groups can depress the whole blood viscosity under low shear rate, the erythrocytic clustering index and the content of fibrinogen obviously. Conclusion QXFMG can improve the hemorheology level of rat with acute blood stasis by depressing the whole blood viscosity under low shear rate, restraining the clustering of erythrocyte and depressing the content of fibrinogen, which is prior to the Compound Danshen piece.

Objective To study the release of synthetic gene expression induced by vasopressin Ⅱ (U Ⅱ ) in hypoxic pulmonary hypertension. Methods Rat model of HPH was establish. RIA was used to observe the different time points of hypoxia in plasma and the dynamic changes of U Ⅱ , ADM content in bronchoalveolar lavage fluid (BALF). The impact of the release of U Ⅱ , as well as the relation-ship among U Ⅱ , ADM and HPH were explored to reveal the role of U Ⅱ in the pathophysiology of HPH. Results Rat HPH model was successfully established. Hypoxia promoted the expression, synthesis and release of U Ⅱ in lung tissue. U Ⅱ involved in the pathogenesis of HPH. HPH took place in the development of U Ⅱand was positive correlated with ADM. Conclusion The two peptides have opposite physiological effects on blood vessel, which suggest that these two peptides play an important role in maintaining the balance between the pul-monary circulation and lung ventilation as well as the stability of pulmonary artery pressure.

Objective To explore the early diagnosis and treatment of bronchopulmonary dysplasia (BPD) in premature. Methods Observing and analyzing the onset ,diagnosis of BPD and the outcome treated by dexamethasone in 12 cases that were hospitalized in NICU during the period from January 1994 to January 1999.The follow up study was done for 6 to 12 months after their discharge. Results All of these 12 infants treated by dexamethasone were cured and discharged. The time period of hospitalization was 31 to 67(50?14) days. The one year follow up study after discharge showed that the prognosis of 11 patients were good, only 1 infant suffered from repeated respiratory tract infection. Conclusion Careful observation on clinical manifestations and lab work should be done to those premature babies who suffered from BPD easily for early diagnosis. The early application of dexamethasone in infants with BPD showed perfect results.

Objective To investigate gene variation and the relationship between gene variation and pathogenicity of transfusion transmitted virus(TTV).Methods The TTV DNA in the serum sample from a blood donor(BD) and a chronic non-A-G severe hepatitis(CSH) patient with TTV infection was amplified by using PCR.The purified PCR product was cloned and 10 clones from each case were sequenced.The sequences were compared among different clones and analyzed by Phylogentic tree.Results There were two different TTV strains in the BD and seven different TTV strains in the chronic non-A-G severe hepatitis patient.The TTV clones in the BD were of G1a subtype and those of the CSH were of G1a and G1b subtype.Conclusion Gene variant of TTV was much more complicated in the CSH patients than that in the BD ones.

Objective To investigate the effect of the choice of the different models of venous indwelling needle on coronary flash dual-source computed tomography (CT) angiography.Methods 120 patients were randomly divided into three groups (40 cases in each group) who were selected to use 18G,20G,22G indwelling needle.They underwent CT coronary imaging.We measured the CT value of left and right coronary artery opening,right vein opening plane and the right ventricular cavity.We also compared the pain of patients during puncture.Results 120 cases were completed inspection.There was significant difference in the strengthen value of left and right coronary artery and CT value of the right ventricle cavity between 18G and 20G.The difference in the strengthen value of left and right coronary artery and CT value of the right ventricle cavity between 18G and 22G was also significantly different.There was no significant difference in all monitoring points between 20G and 22G.Three types of right coronary vein were not statistically significant.Conclusions Model 20G indwelling needle get the best flash dual-source CT image and better patients' acceptance.

Objective The hsa-miR-106b-25 gene cluster is involved in various biological processes of carcinoma .This study aims at a prediction and function analysis of the hsa-miR-106b-25 gene cluster, miR-106b, miR-93, and miR-25, so as to provide some evidence for further studies on the functions of the three miRNAs and the mechanisms of their interaction . Methods We obtained the sequences of miR-106b, miR-93, and miR-25 from the miRBase, predicted their target genes with TargetScan , PicTar, and miRanda, and used 3 or more experimentally verified target genes from the miRTarbase as the gene set for further bioinformatic analysis .We predic-ted the biological processes of the target genes by GeneOntology analysis and enriched KEGG ( Kyoto Encyclopedia of Genes and Genomes) by pathway analysis, produced protein-protein interaction ( PPI) networks with STRING . Results The target genes of the miR-106b-25 gene cluster were significantly enriched in such biological processes as the regulation of macromolecule metabolism , regulation of metabolic process , and cell cycle process , while the KEGG pathway mainly in glioma, melanoma, prostate cancer , and gallbladder carcino-ma.The proteins encoded by the targeted genes of showed complicated interactions , and those encoded by the KAT2B, PTEN, TP53, CDH1, MDM2, E2F1, RB1, and SMAD7 plaid a core role in the interac-tion network. Conc lusoi n MiRNAs of the miR-106b-25 gene cluster regulate the downstream target proteins involved in tumorigenesis by participating in the cell cycle and cancer signaling pathway .

Network examination and learning has become the main form of test and training for nurses, doctors and pharmacists in the field of medicine and health. The system design of Jsp+Servlet+Extjs 4.1+Tomcat server+Mysql database implements the simulation training and learning function of the medical qualification examination by system positioning , functional development and hierarchical construction . Question bank should be developed and optimized according to the examination syllabus and achievement analysis. Test sites, examples, difficulties and so on have been developed into micro courses. With the sup-port of the system, the micro courses and examination questions have been linked together to achieve the organic integration of examination and guidance. This kind of examination system based on network and on-line learning has good compatibility and strong pertinence. It can be perfected according to the application of learning end.

Cardiovascular Diseases ; Humans ; Natriuretic Peptide, C-Type

Objective To understand and identify the molecules related to the natural resistance to Schistosoma japonicum infection in Mirotus fortis. Methods Sera from Mirotus fortis without schistosome infection were collected. The S.japonicum adult worm cDNA library was immunologically screened with the sera. The positive recombinants were identified, cloned, sequenced and analysed with software and internet. Results Seven genes encoding antigens relevant to sera antibodies in Mirotus fortis were cloned and sequenced. These antigens included glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors(SERPIN), 70 kDa heat shock protein(HSP70), 22\^6 kDa membrane-associated antigen, paramyosin (Sj97), cytochrome C and cathepsin B. Conclusion Many protein molecules might have been involved in natural resistance to \{S.japonicum\} infection in Mirotus fortis. The above 7 kinds of molecules may be identified as new candidates of vaccine against \{S.japonicum\} infection.

Patients undergoing major oncological surgery for head and neck cancer (SHNC) have a particularly high risk of nosocomial infections.We aimed to identify risk factors for ventilator-associated pneumonia (VAP) in patients undergoing SHNC.The study included 465 patients who underwent SHNC between June 2011 and June 2014.The rate of VAP,risk factors for VAP,and biological aspects of VAP were retrospectively evaluated.The incidence of VAP was 19.6％ (n =95) in patients who required more than 48 h of mechanical ventilation.Staphylococcus (37.7％),Enterobacteriaceae (32.1％),Pseudomonas (20.8％),and Haemophilus (16.9％) were the major bacterial species that caused VAP.The independent risk factors for VAP were advanced age,current smoking status,chronic obstructive pulmonary disease,and a higher simplified acute physiology score system II upon admission.Tracheostomy was an independent protective factor for VAP.The median length of stay in the ICU for patients who did or did not develop VAP was 8.0 and 6.5 days,respectively (P =0.006).Mortality among patients who did or did not develop VAP was 16.8％ and 8.4％,respectively (P ＜ 0.001).The potential economic impact of VAP was high because of the significantly extended duration of ventilation.A predictive regression model was developed with a sensitivity of 95.3％ and a specificity of 69.4％.VAP is common in patients who are undergoing SHNC and who require more than 48 h of mechanical ventilation.Therefore,innovative preventive measures should be developed and applied in this high-risk population.