1.Quality standard for Duhuo Jisheng Mixturae
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To establish the quality standard for Duhuo Jisheng Mixture (Radix Angelicae Pubescentis, Herba Taxilli, Cortex Fraxini, etc.) METHODS: Herbs in Duhuo Jisheng Mixture were identified by TLC, and osthol content was determined by TLCS. RESULTS: Rhizoma Chuanxiong; Radix Angelicae Sinensis; Radix Saposhnikoviae; Radix Gentianae Macrophyllae; Cortex Eucommiae; Herba Asari; Radix Paeoniae Alba could be determined. Osthol showed a good linear relationship at a range of 0.466 - 3.600 ?g,r= 0.999 2 . The average recovery was 95.4% . CONCLUSION:The methods are available with a reproducibility and can control the quality of this mixture.
2.Limit test for aristolochic acid in Herba Asari by HPLC
Chinese Traditional Patent Medicine 1992;0(06):-
AIM:To study the detection limits of aristolochic acid in Herba Asari preparation by HPLC. METHODS: Whether Asarum hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.) Kitag contained aristolochic acid A or not by HPLC and MS,and the difference in the reference substance and the sample solution was compared. RESULTS: Aristolochic acid A could not be found in A.hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.)Kitag by HPLC,but be found by MS. CONCLUSION:A.hetertropoides Fr.Schmidt ver.Mandshuricum (Maxim.)Kitag contained trace aristolochic acid,but it is difficult to measure aristolochic acid in Herba Asari by HPLC.The baseline noise of the sample solution can interfere with the detection limit of the trace compound compound
3.Study on Xiaojin Cinnabar Floated Sustained Release Preparation
Chinese Traditional Patent Medicine 1992;0(02):-
Objective: To study the floating and sustained release performance of Xiaojin Cinnabar Floated Sustained Release Capsules. Methods: HPMC was used to form the framwork of floated sustained release capsules containing Xiaojin Cinnabar. The content, floating time in vitro and releasing rate were studied.Results: The floating time in vitro was over 3 hours. The total released amount in 3 hours came to 60%. The conventional Xiaojin Cinnabar didn't have the floating performance. Conclusion: Xiaojin Cinnabar Floated Sustained Relegse Capsules is superior to the conventional Xiaojin cinnabar in the sustained release performance.
4.A case report of young male benign myocarditis.
Lin-lin ZHANG ; Yu-jie ZHOU ; Yu-yang LIU
Chinese Journal of Cardiology 2009;37(5):463-464
Adult
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Humans
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Male
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Myocarditis
6.Expression of phosphoinositide 3 kinase in rats with acute lung injury induced by oleic acid
Yan LIN ; Yan LIU ; Senyang YU
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
Objective To study the expression of phosphoinositide 3 kinase (PI3-K) and its relationship with lung cell apoptosis in rats with acute lung injury induced by oleic acid. Methods 60 healthy male SD rats were divided into 5 groups randomly, with 12 rats for each group. 0.25ml/kg oleic was injected into the tail vein in 4 oleic groups in which the rats were respectively sacrificed after 1h, 2h, 4h, 6h and 24h by exsanguination. In the control group 0.25ml/kg of saline was injected intravenously and sacrificed 2h later. Arterial blood gas, left lung wet/dry ratio, lung index, protein content of BALF, and pulmonary permeability index (PPI) were determined. Fas-L expression of lung tissue was determined by immunohistochemical method and protein expression of PI3-K was determined by Western blot and immunohistochemical method. Results PaO_2 was lowered, and left lung wet/dry ratio, lung index, and protein content of BALF and PPI were increased obviously after oleic acid injection. Fas-L and PI3-K in bronchus epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells were highly expressed in the lung of ALI rats, and peak value of PI3-K appeared earlier than Fas-L. Conclusion Fas-L related apoptosis of bronchial epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells participated in the pathogenesis of earlier period of ALI through PI3-K transduction pathway.
7.Effects of dexamethasone on cell signal transaction system ERK and PI3-K of rat with acute lung injury
Yan LIN ; Yan LIU ; Senyang YU
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Objective The effects of dexamethasone on cell signal transaction system ERK and PI3-K were studied in acute lung injury (ALI) rat in order to provide a theoretical basis for clinical treatment. Methods 36 healthy male SD rats were randomly divided into 3 groups, 12 rats for each group: injured group, dexamethasone treated group and control group. 0.25ml/kg oleic acid was injected via caudal venous into each animal in the former two groups to form ALI models. Then, for dexamethasone treated group, each animal received 1.0mg/kg of dexamethasone 15 min after oleic acid injection; the same amount of saline was given to the animals in injured group, and the animals in control group were injected the same amount of saline at the last two times. All animals were killed by common carotid artery bleeding 2 hours after the last injection. The indexes of PaO2, wet/dry weight of left lung, pulmonary permeability and lung pathology were investigated. The expression of PI3-K, ERK and P-ERK were determined by Western blot and immunohistochemical method. Results PaO2 declined in the animals of injured group, while left lung wet/dry ratio and pulmonary permeability increased obviously, and pulmonary edema and transparent film were observed in injured lung pathology. Compared to injured group, those indexes were improved in dexamethasone treated group. PI3-K, ERK and P-ERK were highly expressed in bronchus epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells of the animals in injured group, the expression of PI3-K, ERK and P-ERK were reduced in dexamethasone group, but the expression in the both groups were still higher than that in control group. Conclusions The higher expression of PI3-K and ERK were involved in the regulation of earlier period of ALI, and dexamethasone may improve ALI by repressing the high expression of PI3-K and ERK.
8.Clinical study on chemotherapy combined radioactive seed intersti-tial brachytherapy for locally advanced non-small cell lung cancer
Xiulan LIU ; Yingna BAO ; Yu LIN
Chinese Journal of Clinical Oncology 2013;(16):992-996
Objective: To evaluate the clinical effect and adverse reactions of radioactive seed interstitial brachytherapy com-bined with etoposide (EP) regimen concurrent chemoradiotherapy treatment for locally advanced non-small cell lung cancer. Methods:All 24 cases of locally advanced non-small cell lung cancer received three-dimensional conformal intensity modulated radiation therapy by using 6 MV X-ray to obtain 95%60-66 Gy/30-33 F planning target volume. All cases received radiation therapy five times a week. EP regimen chemotherapy concurrent with radiotherapy was given to 24 patients. The therapy included VP-16, 60 mg/m2 intravenous in-fusion for 1 d to 5 d, and DDP 50 mg/m2 intravenous infusion on the 1st, 8th, and 28th therapy day. Chemotherapy with EP regimen was given for four cycles, in which two cycles were given during radiotherapy and the remaining cycles were completed after radiotherapy. The patients were reexamined three months after concurrent chemoradiotherapy was completed. Patients with residual tumor, con-firmed via positron emission tomography/computed tomography, underwent 125I radioactive seed implantation interstitial brachytherapy to complement the dose of tumor. Results: The response rate was 83.3% (20/24); the local control rates of 3, 6, 9, 12, 18, and 24 months were 87.5% (21/24), 83.3% (20/24), 75.0% (18/24), 70.8% (17/24), 58.3% (14/24), and 50.0% (12/24). The median survival was 20.2 months. The one-year survival rate was 62.5%, and the two-year survival rate was 37.5%. The following main toxicities were observed:the incidence of radiation-induced lung injury was 25%;the incidence of radiation esophagitis was 33.3%;the incidence of grades Ⅰ to Ⅱ gastrointestinal reactions was 82.3%; the incidence of reducing neutropenia was 87.5%, in which the incidence of gradesⅠtoⅡwas 75.0%, gradeⅢwas 12.5%, and gradeⅣwas 0%. Conclusion:EP regimen concurrent radiotherapy and chemo-therapy combined with radioactive seed interstitial brachytherapy for locally advanced non-small cell lung cancer is effective and has few serious adverse reactions, thus making this approach worthy of promotion.
9.Progress in single nucleotide polymorphism with cancer susceptibility and its clinical application
Chunling LIU ; Dianke YU ; Dongxin LIN
Chinese Journal of Laboratory Medicine 2012;35(7):579-584
Single nueleotide polymorphisms (SNPs) are the most common genetic variants in human genome.Candidate gene,genome-wide association studies (GWASs) and exome sequencing which base on SNPs have made a great progress in identifying cancer susceptibility.The development and application of high resolutions in SNPs has played an important role in clarifying the mechanism,prevention,diagnosis and targeted therapy in cancers.
10.Analysis of Economic Factors on Prevention and Cure of Childhood Asthma in Qingdao Area
Rongjun LIN ; Yongfeng YU ; Xiaomei LIU
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(01):-
Objective To probe into the medical cost and economic losses of childhood asthma. Methods Total 200 of asthmatic children aged 2~16 yrs were diagnosed according to asthma OPD and enrolled in this study from the Affiliated Hospital of Medical College of Qingdao University during 2005—2006. All of them were taken the epidemiologic and economic survey. Results Among them, 184 were from kindergartens, primary or middle schools. There were totally 7 629 days for the asthmatic children absent from class per year because of illness. The overall direct economic loss was RMB 798 467.00 and indirect loss was RMB 210 997.80 yearly. Conclusion Asthma not only brings about great impact on children physical and mental health, but also exerts massive economic loss on the society and families. The whole society and medical workers should pay more attention to it and try to reduce its harm to a relatively low level.