1.Preparation and Clinical Application of Yousaiqing Suppository
Youping LIU ; Zhenhua YANG ; Changxiao LIU
China Pharmacy 2001;12(6):338-339
OBJECTIVE: To prepare Yousaiqing suppository for treating ulcerative colitis and observe its clinical therapeutic effect.METHODS: The ingredients of suppository, PASA and berberine HCl, were determined with RP-HPLC and the therapeutic effect was observed in comparison with sulfasalazine suppository.RESULTS: This method of quality control was feasible and the effective rate for ulcerative colitis amounted to 70.7% .CONCLUSION: The quality control method is simple, reliable and suitable to routine analysis and the therapeutic effect is satisfactory.
2.Research progress of medical therapies for chronic myeloid leukemia
Suxiao LIU ; Youping WANG ; Lin CUI
Chinese Journal of Biochemical Pharmaceutics 2016;36(8):157-159
Chronic myeloid leukemia(CML)is a pluripotent stem cell disease characterized by the proliferation of myeloid cell.Ph chromosome has been found in 95% of CML patients.BCR-ABL fusion protein expressing on Ph chromosome displayed constitutive tyrosine kinase activity.The fusion protein could phosphorylate and activate downstream substrates continually in the process of cell signal transduction,and then cells proliferate and migrate constantly,resulting the occurrence of CML.In recent years, people have been tried various methods to cure CML, which included developing some chemotherapy drugs and herbal extracts,as well as designing and synthesizing the inhibitors targeting BCR-ABL tyrosine kinase.Clinical studies showed that these drugs have been achieved remarkable effects.This paple aimed to review for drugs treated in CML.
3.Determination of dehydroxypresenegenin in Polygalae tenuifolia by HPLC
Youping LIU ; Deguang WAN ; Ying SONG ;
Chinese Traditional and Herbal Drugs 1994;0(09):-
Object To establish an HPLC method for the determination of dehydroxypresenegenin for the quality control of Polygalae tenuifolia Willd. Methods Dehydroxypresenegenin was separated by ODS column (125 mm ? 4 mm, 5 ?m) with a mobile phase of methanol 0 05% phosphoric acid (74∶26) and detected at a wavelength of 210 nm. Results The calibration curve was linear in the range of 0 585~11 7 ?g,with a correlation coefficient of 0 999 99 . The average recovery was 99 21% (n=5), and RSD=0 25% (n=5). Conclusion The method is simple and accurate, with good repeatability and can be used for the quality control of Polygalae tenuifolia Willd and compound preparations of Radix Polygalae.
5.Study on Chemical Constitutes of Ligusticum chuanxiong and Content Determination of Ligustilide
Ling ZHANG ; Youping LIU ; Min LI ; Huiyong LI ; Jingxian FAN
China Pharmacy 2005;0(15):-
OBJECTIVE:To study the chemical constitutes of Ligusticum chuanxiong and to establish the method for the content determination of ligustilide. METHODS:The compounds were extracted and percolated by ethanol. Then the samples were separated using silica gel and identified by 1H-NMR,13C-NMR data.HPLC was used to assay the contents of ligustilide. RESULTS:Three compounds were isolated and their structures were identified as Z-ligustilide,Z-6,8',7,3'-diligustilide and Z,Z'-6,6',7,3'a-diligustilide. The contents of ligustilide were no less than 0.70%.CONCLUSION:The separation method can be used to prepare high-purity ligustilide reference substance. And the determination method can be used for quality control of L. chuanxiong.
6.Determination of Vitamin B_1 and Vitamin B_6 in Gengnianling Capsules by HPLC Simultaneously
Yuan HU ; Bing WU ; Lin CHEN ; Youping LIU
China Pharmacy 2005;0(21):-
OBJECTIVE:To determinate vitamin B1 and vitamin B6 in Gengnianling capsules by HPLC simultaneously .METHODS: The separation was performed on Hypersil-ODS C18 column, methanol - sodium hexanesulfonate solution(20 : 80) was used as mobile phase with a flow rate of 0.8ml/ min and detection wavelength of 280nm.RESULTS: Linear correlations with peak area scores were achieved when the sample size of vitamin B1 and vitamin B6 were with a range of 0.884?g-2.652?g (r = 0.9 999) and 0.714?g-2.142?g(r = 0.9 999) .respectively, the average recovery of which were 95.87%(RSD = 0.82%) and 101.96% (RSD = 0.86%), respectively .CONCLUSION: The method is simple, accurate and it can be used for quality control of Gengnianling Capsule.
7.Clinical study of combined interventional embolization for the treatment of portal hypertention
Xuejian LIU ; Enshan LI ; Youping MA ; Chunqing ZHANG
Chinese Journal of General Surgery 1993;0(01):-
0.05).(4) Free pressure of portal vein(FPP) significantly declined at post-operation in the two groups(P
8.Clinical trial registration system and evidence-based medicine
Taixiang WU ; Youping LI ; Guanjian LIU ; Jing LI
Journal of Integrative Medicine 2007;5(3):229-33
The authors briefly introduced the management of clinical test for new drug development, clinical trials for drugs prepared in hospital and post-market drugs, and other types of clinical trials. The mechanism of WHO International Clinical Trial Register Platform (WHO ICTRP), Chinese Clinical Trial Register (ChiCTR) and Chinese Clinical Trial Registration and Publishing Collaboration (ChiCTRPC) were also introduced. The authors suggested the trialists to practice the basic philosophy of evidence-based medicine as the rules of their thought and action, and considered that this is the inner guarantee system for the validity of clinical trials.
9.Research Progress on Fungi and Mycotoxin Infection of Chinese Medicinal Materials
Xin ZHANG ; Fu WANG ; Hongping CHEN ; Youping LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(11):2381-2388
Chinese medicinal materials are likely to be polluted by various kinds of fungal toxins during the plantation,collection,transportation and storage,which results in the possibility that quality,safety and treatment effect fails to meet the requirement of safe medicine.This paper reviewed the domestic and overseas studies on the pollution of fungus and fungal toxins in herbs,and made an introduction to the limit standard of fungus and fungal toxins.This paper discussed the importance to establish the inspection standard of various fungal toxins in Chinese medicinal materials and their limit standards to provide references to medication safety and improvement of quality control standard system of Chinese medicinal materials.
10.Effects of acupuncture on the ratio of CD4+ CD25+ regulatory T cells and expression of transcription factor Foxp3 in patients with septic shock
Youping JIN ; Liping JIANG ; Jing GUO ; Yongjun TAO ; Jianjun LIU
Chinese Journal of Primary Medicine and Pharmacy 2017;24(18):2784-2787
Objective To study the effect of acupuncture on the ratio of CD4+ CD25+ regulatory T cells and expression of transcription factor Foxp3 in patients with septic shock.Methods Sixty-four patients with septic shock were randomly divided into two groups by using the random number table method.Acupuncture group (34 cases) was treated with both western medicine and acupuncture,and control group(30 cases) was treated with western medicine.The period of treatment was 7 days.After treatment,the ratio of CD4+ CD25+ T cells in peripheral blood was determined by flow cytometry.And the expression of Foxp3 mRNA in peripheral blood was detected by quantitative real time PCR.Results After treating for 7 days,the ratios of CD4+ CD25+ Treg cells and CD4+ CD25+ Foxp3 + Treg cells were (20.23 ± 1.12) % and (78.70 ± 7.65) % respectively in peripheral blood of the control group,which in the acupuncture groupwere (17.32 ± 0.78) % and (68.53 ± 8.01) %,the differences were statistically significant between the two groups(t =2.587,2.749,all P < 0.05).The levels of Foxp3 mRNA in peripheral blood were (1.21 ±0.02) and (1.02 ± 0.04) in the control group and acupuncture group,the difference was statistically significant(t =2.119,P < 0.05).Conclusion Acupuncture can adjust immune status of patients with septic shock by reducing the ratio of CD4+ CD25+ Treg cells and down-regulating the expression of Foxp3 mRNA.