Objective:To evaluate the current situation and the developing trend of 5-HT3 receptor antagonists used in 25 hospital of Wuhan during the period of 2003～2005 in order to provide reference for clinical doctors and pharmacists. Method:The 5-HT3 receptor antagonists were statistically analyzed in respect to the purchase sum of money,DDDs and daily costs. Result:During the 3 years, purchase sum of money of 5-HT3 receptor antagonists were yearly increasing. The share of the market of domestic drugs showed an increasing tendency,while the imported drugs decreased year by year. Granisetron, ondansetron and tropisetron were much more frequently used than others. Conclusion:The market of the oral agents of 5-HT3 receptor antagonists will further expand in China.

Objective To explore the causes of misdiagnose of ectopic pregnancy by retrospective analysis in our hospital cases,in order to increase the diagnose and curative level in ectopic pregnancy.Methods Clinical data of the misdiagnosis of 96 cases from the 357 patients of ectopic pregnancy in our hospital from year 1993 to 2002,including materials of histories ,symptoms signs and laboratory examinations were retrospectively analyzed.Results The misdiagnosis rate of ectopic pregnancy in our hospital was 26 9%, most of them were treated as early pregnancies, threatened abortions etc. The causes of misdiagnosis were neglectful of asking essential history, no overall physical examination and misleading by the laboratory results.Conclusions The effectively preventive measures against misdiagnosing of ectopic pregnancy were strengthen vigilance of ectopic pregnancy,familiar with the knowledge of atypical ectopic pregnancy and the essential examinations on gravida.

Consensus ; Humans ; Infant, Newborn ; Jaundice, Neonatal ; diagnosis ; therapy ; Practice Guidelines as Topic

Electrocardiography ; Humans ; Myocardial Infarction ; physiopathology ; Myocardial Perfusion Imaging ; methods

As our country lack professionals of public health,higher demand to the preventive medicine is further made with the transformation of medical model and the construction of the public health system,and the reformation in preventive medicine education of higher learning is explored to cultivate the practical professionals of public health by modulating education program and courses,modifying the cultivating plan,emphasizing the knowledge of liberal arts and management,strengthening the cultivation of ability and combining theory with practice.

BACKGROUND:The ligamentum flavum is important to connect the rear of the spine. If the flexibility of the ligamentum flavum is decreased due to degeneration, in the role of various factors, it can reduce the effective space of the spinal canal to induce spinal cord and nerve root compression, thereby resulting in neurological symptoms, even paralysis or residual disease. OBJECTIVE:To study the mechanism of over-dose fluoride on the degeneration and ossification of the ligamentum flavum in rats. METHODS:Thirty male Sprague-Dawley rats were randomly divided into two groups: experimental group was given distiled water containing NaF (mass fraction 10-4) and control group was given the same amount of distiled water. After 3 and 6 months, the bone mineral density, Ca2+ and P3+ contents in serum and bone tissue and serum alkaline phosphatase activity were detected, respectively. Meanwhile, the ligamentum flavum specimens were taken for X-ray examination and histopathological observation. RESULTS AND CONCLUSION: In the experimental group, chalk stripes or spots occurred in the lower incisors of five rats at 3 months after treatment and dental fluorosis that was mostly in chalk color was found in al the rats at 6 months after treatment; moreover, the bone density of the lumbar spine significantly increased (P < 0.05). Compared with the control group, the contents of serum Ca2+ were significantly higher, while the contents of P3+were significantly lower in the experimental group at 3 and 6 months after treatment (P < 0.05); the serum alkaline phosphatase activity was also significantly higher in the experimental group than the control group at 3 and 6 months after treatment (P < 0.05). X-ray films, at 6 months after treatment, showed that the bone density of the lumbar vertebra was increased obviously; pathological observation showed that the number of elastic fibers in the ligamentum flavum was reduced further, a large amount of colagen fibers, fibroblasts and calcium deposition were found, and the ossification of the ligamentum flavum was visible at the lamina attachment site. These findings indicate that the over-dose fluorides can lead to the degeneration and ossification of the ligamentum flavum in Sprague-Dawley rats, which may plan an important role in the ossification of the ligamentum flavum.

Objective To investigate the effects of long term exposure of 18F-FDG-PET/CT X-rays on rabbit immune system.Methods The rabbits were irradiated with 18F-FDG-PET/CT every day and this irradiation treatment continued for 3 months.At each month,the total number of white blood cells,thymus index and spleen index,phagocytic index,immune globulins,TNF-α and IL-1 in peripheral blood and serum were detected.Results After long-term 18F-FDG-PET/CT irradiation,the total number of white blood cells increased,the thymus and spleen index decreased (t =3.144-72.903,P ＜ 0.05),the phagocytic index and phagocytic percentage decreased after 1 or 2 months(t =2.719-11.859,P ＜ 0.05),and the number of immune cells marked with CD4 + increased but CD8 + decreased(t =6.433-29.877,P ＜ 0.05).In addition,the level of immune globulin in the serum decreased,but the expressions of TNF-α and IL-1β increased (t =2.720-28.775,P ＜ 0.05).Conclusions 18F-FDG-PET/CT has some damage effects on the immune system of male Japanese big ear rabbit,suggesting that the negative effects of PET-CT should be paid close attention to during clinical application.

The main clinical symptoms of Parkinson disease (PD) are resting tremor, muscle rigidity and bradykinesia. There is currently no effective treatment for PD, and dyskinesia symptoms affect the quality of life of patients with PD. The Chinese medicine therapy used for reinforcing kidney and activating blood circulation in treatment of PD was reported to achieve good clinical effects.

Objective To explore the expression of breast cancer susceptibility gene 1 (BRCA1) in human colorectal cancer and its correlation with the efficacy of platinum-based chemotherapy.Methods Seventy-eight cases of colorectal cancer patients treated with platinum-based chemotherapy after surgery were collected.The surgical specimens of them were taken.Fourteen normal colonic mucosa specimens and 12 non-cancerous tissues of colorectal cancer specimens were obtained.The expression of BRCA1 in tissues was detected by immunohistochemistry and analyzed by x2 test.The colorectal cancer patients were followed up for survival time.Comparison of survival analysis was performed by Kaplan-Meier survival curves and Log-rank test.Results The positive rate of the BRCA1 expression in colorectal cancer tissue (52.6 ％,41/78) was lower than that of para-cancer tissue (11/12,x2 =6.518,P=0.011) and normal colonic mucosa tissue (13/14,x2 =7.949,P=0.005).The poorer the differentiation of colorectal cancer,the lower the positive rate of BRCA1 (x2=14.160,P=0.001).The median disease-free survival time of BRCA1 negative colorectal cancer patients was 51.0 months (95 ％ CI:47.7 to 54.4 months),which was longer than that of BRCA1 positive patients (45.0 months,95 ％CI:36.6 to 53.4 months,x2 =4.367,P=0.032).Conclusions Receiving oxaliplatin based chemotherapy may be a survival benefit for BRCA1 negative colorectal cancer patients.The expression of BRCA1 may be an index for chemotherapy options and prognosis evaluation for colorectal cancer patients after surgery.

AIM　To investigate the therapeutic effect of boanmycin (BAM, bleomycin A6) on colon carcinoma 26 and its hepatic metastasis in mice. METHODS　A series of models including subcutaneous transplantation, orthotopic transplantation in cecum subserosa, intra-hepatic transplantation of tumor, and intra-splenic transplantation of tumor accompanied with hepatic metastases were employed. In addition, LEICA Q 500IW image analysis system was used to determine the area of metastatic lesions in the liver in histopathological sections. RESULTS　BAM at 5 mg*kg-1 and 2.5 mg*kg-1 inhibited the growth of subcutaneous tumors by 78.7% and 61.9%, and the orthotopic tumors by 99.4% and 90.0%; and the intra-hepatic tumors by 86.9% and 75.7%, respectively. Determined by the numbers of metastatic nodules, BAM at 10 mg*kg-1 and 5 mg*kg-1 inhibited hepatic metastases from intra-splenic transplantation by 97.6% and 56.8%; and evaluated by image analysis of metastatic lesions, by 100% and 63.3%, respectively. CONCLUSION　Boanmycin is highly effective in a panel of models including the subcutaneous, orthotopic, intra-hepatic transplanted tumors, and hepatic metastases of murine colon carcinoma 26.