Objective To investigation the relationship between health management and prognosis of chronic prostatitis. Methods Three hundied and forty-six cases of chronic prostatitis were divided into two groups. The control group treatment was local washing and medicine for three months. The experiment group treatment was local washing, medicine, and health management for three months. NIH-CPSI was used for the results. Results There was no significant different between the experimental group(165 cases)and the control group(154 cases)before treatment in NIH-CPSI. The effective rate of the the experimental group was 88.5%, and that of the control group was 64.9% after treatment. It is effective for cure and the prognosis of the experiment was better than the control (P<0.05). Conclusion It is suggested that it is more effective for the prognosis of chronic prostatitis after the intervention of health management.

An inflatable liquid epidural balloon was used to simulate an intracranial space-occupying lesion.Two groups of dogs with 8 in each were employed.Under different conditions and time intervals,intracranial pressure(ICP)was continuously recorded,and pressure-volume index(PVI),volume-pressure response(VPR),and cerebrospinal fluid pulse pressure(CSFPP)were measured silmutaneously.It was found that PVI was in significant negative linear correlation with ICP,while VPR and CSFPP in significant positive linear correlation with IVP(P

Objective To introduce the wavelet zero -cross stimulation algorithm and based on which to design and implement the cochlear implants. Methods Through DSP and ICETEK-VC5416, the WZS speech coding strategy was simulated, and also the presented WZS strategy was compared to CIS strategy by hardware implementation and computer simulation. Results The processed audio signals, output from eight channels, were synthesized and played through loudhailer and could be heard with high fidelity. Conclusion The wavelet zero-cross stimulation(WZS) strategy, which generates stimulating pulsatile series at zero-cross points in the domain of wavelet transform, can keep the temporal correlation and coherence of speech remained in stimulating pulsatile series and reflects the characteristic of temporal coding for cochlea.

Objective:To study the effects of LeFort I osteotomy in the treatment of old maxillary fracture.Methods:30 patients with old maxillary fracture were treated with LeFort I osteotomy,the rehabilitation of occluding relation and masticatory function were observed after operation.Results:28 patients had well-pleasing occluding relation and masticatory function; 2 patients dissatisfied with their masticatory function, because of unbalance of nerve-muscle after their occlusal plane were rebuilt.Conclusion:LeFort I osteotomy can be a method in the treatment of old maxillary fracture.

Objective To evaluate the abilities and thresholds of stroke volume variation (SVV) and pleth variability index (PVI) in predicting fluid responsiveness during increased intra-abdominal pressure.Methods 28 patients undergoing laparoscopy-assisted radical gastrectomy were selected.PV1 was continuously displayed by the Masimo.Radical 7.All patients were also monitored with Vigileo/FloTrac system.Haemodynamic data such as MAP,HR,SVI,SVV,PI,PVI and C VP were recorded before and after volume expansion(HES 6％,7ml/kg).Fluid responsiveness was defined as an increase in SVI≥ 15％ (△ SVI ≥ 15).Results The SVV threshold of 9.5％ before volume expansion was able to diserimihate the responders from the non-responders with a sensitivity of 100％,and a specificity of 63.6％.The threshold for PVI was 14.0％,the sensitivity of 100％ and specificity of 81.8％ were obtained.There was no significant difference between the area under the receiver operating characteristics (ROC) curves of SVV and PVI(0.981,0.939,respectively),and there was significant correlation between the baseline SVV and the baseline PVI(r =0.740,P ＜ 0.01).Conclusion SVV and PVI can predict fluid responsiveness accurately during increased intra-abdominal pressure,the baseline SVV is correlated well with baseline PVI,and the ability of SVV and PVI in predicting fluid responsiveness is similar.

Purpose To investigate the correlation of the expression of Cyclin D1 and BRAF ( V600E) mutation in papillary thyroid carcinoma ( PTC) and their clinical significance, and to analyze their role in the pathogenesis of PTC. Methods The expression of CK34βE12 and MC protein was detected by immunohistochemical EnVision method in 52 cases of PTC and 52 cases of thyroid benign lesions (25 cases of nodular goiters, 15 cases of Hashimoto’s thyroiditis, 12 cases of follicular adenoma), and the mutation status of BRAF gene protein in above specimens were detected by polymerase chain reaction and DNA sequencing. Expression of Cyclin D1 and BRAF (V600E) mutation in papillary thyroid carcinoma and their correlation clinicopathologic features were also analyzed. Results The positive rate of Cyclin D1 in 88 cases of PTC was 84. 6%,and the immunoreactivity score (4. 6 ± 2. 4), compared with thyroid be-nign lesions (1. 3 ± 1. 6), was statistically significant difference (t=8. 525, P<0. 01). Expression of Cyclin D1 was closely related with lymph node metastasis, capsular invasion, tumor stage Ⅲ+Ⅳ and BRAF (V600E) mutation (P<0. 05), but not related with age, gender, number and diameter of tumor nodules. In 52 cases of PTC BRAF gene mutation rate was 63. 5%, but in thyroid benign lesions mutation rate was 0, with significant difference (P<0. 01). The BRAF mutation is closely related with lymph node metastasis, capsular invasion and tumor stage Ⅲ+Ⅳ(P<0. 05), but not related with age, gender, number and diameter of tumor nodules. The expression of Cyclin D1 in BRAF (V600E) mutation group was stronger, the positive expression rate was significantly higher than that of wild group (100% vs 57. 9%), and expression of the mean score was higher than that of the wild group (5. 7 ± 1. 6 vs 4. 0 ± 2. 5);the comparison between the two groups showed statistical difference (t=2. 652, P<0. 05). Conclusions The expression of Cyclin D1 and BRAF (V600E) mutation are positively related, which are also closely related with the occurrence and development of PTC, and can be used as an index to predict the invasion and metastasis in PTC.

Objective To analyze the implementation effects of peer-assisted learning on critical thinking ability of undergraduate nursing students. Methods A total of 411 college nursing students were selected with 203 students of Class One, Class Three, and Class Five from Grade 2012 majoring in nursing were taken as the control group, 208 students of Class Two, Class Four, and Class Six as the experimental group. In the course of basic nursing practice, the control group was trained by the traditional practice, and the experimental group was trained by the way of peer mutual aid. Critical thinking disposition inventory Chinese version (CTDI-CV) was adopted to evaluate the level of critical thinking ability of the nursing students of both groups. SPSS 17.0 was used and the data of the two groups were compared by t test. Results The score of experimental group students' critical thinking ability and the score of 7 dimensions including their seeking truth, analysis ability, self-confidence, inquisitiveness, cognitive maturity, open mind and systematic ability were higher than those before the experiment (P<0.05). The total score of the experimental group students' critical thinking ability and the score of 5 dimensions including seeking truth , analysis ability, self-confidence, curiosity and cognitive maturity were higher than those of the control group (P<0.05). Conclusion Peer-assisted learning is beneficial to the improvement of critical thinking ability of nursing undergraduate students.

To evaluate the influence of different brands of syringes on performance parameters and application quality of syringe pump, we carried out matching experiment of infusion/syringe pump analyzer Rigel Multi-Flo, made by ALK Co. in Sweden, for 3 different types of syringes to measure the flow rate, occlusion alarm pressure, occlusion time and bolus volume of 26 syringe pumps. Data of quality control were analyzed with statistical method. The results demonstrated that there were significant differences in the flow rate, occlusion alarm pressure, occlusion time and bolus volume (P<0. 01) between those of common syringes and original syringes. This study points out that the health care providers should select compatible syringes for syringe pumps, which provides evidence and guidance to assure the reasonable and safe application of syringe pumps in clinical practice.
Infusion Pumps ; Quality Control ; Syringes

BACKGROUND: Previous research has indicated that both insulin-like growth factor Ⅰ (IGF-Ⅰ) and basic fibroblast growth factor (bFGF) play an important role in cell proliferation and differentiation. However, the effect on biological characteristics of human dental papilla mesenchymal cells (hDPMCs) still remains unclear. OBJECTIVE: To research the effect of IGF-Ⅰ and bFGF on the proliferation and differentiation of hDPMCs.METHODS: hDPMCs were isolated and cultured in DMEM/F12 culture media containing 1% or 10% fetal bovine serum. The fourth-passaged hDPMCs were incubated in culture media containing 0, 0.1, 1, 10 and 100 μg/L bFGF and 0, 25, 50, 75 and 100 μg/L IGF-Ⅰ(0 μg/L as control group), respectively. At 96 hours after culture, proliferative activity was measured with MTT assay. The corresponding growth factor culture media were used in 10 μg/L bFGF group, 100 μg/L IGF-Ⅰgroup, bFGF + IGF-Ⅰ group, and control group, respectively. At days 1, 3, 5, and 7 after culture, the proliferative activity was detected using MTT assay, and alkaline phosphatase (ALP) activity was measured using modified enzyme kinetics method. RESULTS AND CONCLUSION: At the 0-100 μg/L mass concentration scope, both bFGF and IGF-Ⅰcould accelerate proliferation of hDPMCs, and the proliferation ability of bFGF was superior to that of IGF-Ⅰ; moreover, the combination of bFGF and IGF-Ⅰcaused a synergetic action to proliferation of hDPMCs. The maximal valid concentration of bFGF was 10 μg/L, and the maximal action concentration of IGF-Ⅰwas 100 μg/L. At 0-7 days, the effect of bFGF on the ALP activity of hDPMCs was not obvious, but the effect of IGF-Ⅰon ALP activity of hDPMCs became greater with the time passing; furthermore, the combination of bFGF and IGF-Ⅰcould generate a synergetic action on increasing the ALP activity.

The construction of on campus practice base is crucial for improving the quality of practical teaching.We put forward a construction pattern of‘grid type' pharmacy teaching comprehensive laboratory.It covers three-leveled experimental platform and professional teaching laboratory according to the research and development process of new drug.It also forms the communication network between learning and working demand of students.This provides new ideas for the construction of pharmacy teaching laboratory in newly-built university.