Objective To study the compound oral contraceptives(COC)application in patients with missed abortion after.Methods 600 cases of missed abortion from March 2016～2017 year in March in our hospital,were randomly divided into observation group(n=300)and control group(n=300),the observation group was treated with compound oral contraceptive therapy,the control group were given routine treatment,compared two groups of patients.Results The observation group of patients with vaginal bleeding time,menstrual recovery time was significantly shorter than the control group(P<0.05),endometrial thickness was significantly greater than the control group(P<0.05),the incidence of Intrauterine Residue and uterine cavity adhesion rate,pelvic infection rate was significantly lower than the control group(P<0.05); the two groups of patients with postoperative weight change in March the amount of vaginal bleeding,compared no significant difference,no statistical significance.Conclusion The application of compound oral contraceptives in the treatment of missed abortion patients after curettage,shorten the time of vaginal bleeding,reduce the amount of vaginal bleeding,reduce the incidence of postoperative complications and promote postoperative recovery of menses.

OBJECTIVE:To study the safety of polylatic acid used as sustained-release drug carrier in brain.METHODS:The toxicity and apoptosis of in vitro primary cultured cortical neurons in rats were evaluated using CCK-8 and Hoechst33258 staining after treated with polylatic acid and compared with the normal control group.Self-made polylatic acid blank tablet was implanted into rat brain and after 30 days the neuronal function was assessed through behavior ability testing,HE staining and Nissl staining,compared with sham groups and normal control group.RESULTS:When neurons were cultured for 7,9 and 12 days in polylatic acid group,there was no significant difference in absorbance value of CCK-8 and apoptosis rate between normal control group and polylatic acid group.Light inflammatory was observed in rat brain tissue in polylatic acid blank tablet group after 30 days,as compared with sham group.But the morphology,number and function of neuron had no obvious change.CONCLUSION:Polylactic acid has no significant inhibitory and inducing apoptosis effect on in vitro cultured cortical neurons and also has no obvious toxicity on brain tissue.

Objective The effect of the pesticides on sister-chromatid exchange and micronuclei frequency of garlic root tip cells was researched. Methods The garlic root tips were treated with 13.65 mg/L phoxim, 25 mg/L lambda-cyhalothrin respectrely.With the positive and negative control, the garlic root tips were treated with 2%CP and distilled water respectively. Sister-chromatid exchange(SCE) and micronucle(iMCN)frequencies were calculated. Results As for the SCE frequency,lambda-cyhalothrin was the same as the negative control, metaldehyde and phoxim were more than the negative control (P

Objective:To explore the effects of VEGF and CD44V6 on breast cancer MCF-7 cells by NDGA.Methods:MCF-7 cells in the logarithmic growth state were selected and measured.There were four cell groups(concentration:0,1,10,100?mol/L)in the experiment.After 48h treatment of NDGA,the expression of VEGFmRNA was measured by RT-PCR and the expression of CD44V6 was examined by flow cytometry.Results:After treatment of NDGA,the expressions of VEGFmRNA and CD44V6 in NDGA groups were significantly lower than that of the control group(F=2996.13,P

AIM: To develop a method for optimizing of supercritical fluid extraction (SFE) of paeoniflorin. METHODS: On the basis of one-factor test and the Box-Behnken central-composite design response surface with 3 factors and 3 levels was adopted. A second order quadratic equation for SFE of paeoniflorin was built. Response surface and contour were graphed with the extraction rate of paeoniflorin as the response value. Based on the analysis of the response surface plots and their corresponding contour plots,the effects of extraction pressure,extraction temperature,and methanol concentration were explored. RESULTS: By using the new method discussed above, the optimum extraction condition was obtained as follows: 18. 9 MPa extraction pressure,extraction cosolvent temperature 59. 9 ℃,68. 5% methanol was chosen as cosolvent with the flow rate of 0. 4 mL/min,and extraction time 60 min. CONCLUSION: Under the optimized condition,extraction rate of paeoniflorin on theoretical value is 7. 85 mg/g.

Objective To investigate the changes of region cerebral homodynamic and cerebral vascular autoregulation in traumatic brain injury(TBI) by dynamic CT perfusion(CTP) imaging in rat.Methods Forty-five SD rats underwent CTP,including 10 control rats and 35 TBI rats,on 1,6,24 h,and day 3,7,14,21 after TBI.The region of interest(ROI) was located near traumatic area within 2 mm and mirror area of relative brain hemisphere.False color pictures and dynamic rCBF,rCBV,MTT and PS were obtained in different time.Pathological examination was also done.Results The rCBF,rCBV on 1 h after TBI was lower than that of control group,and significantly higher on 6-24 h after TBI.They reached the peak on day 3.PS had a similar change compared with rCBF,rCBV,but a longer duration.MTT showed to moderate changes except an isolated tide on 3 days after TBI.The rCBF on mirror area of relative brain hemisphere was almost stable except 3 days after TBI,which was lower than that of control group.Meanwhile,the rCBV on mirror area of relative brain hemisphere was normal.Conclusion CTP is an advanced technique to investigate the changes of region cerebral homodynamic and cerebral vascular autoregulation in TBI.The early hypoperfusion and next hyperperfusion around traumatic areas after TBI suggest that the dysfunction of cerebral vascular autoregulation should be committed to the change of homodynamic in TBI.

During the development of pancreatic cancer,the local and whole immune state of the patients is constantly changing,and through which the cancer cells escape from the immune attack.The main immunologic mechanism includes the induction of tumor immune suppression cells and producing the related cytokines.The changes of the whole immune status include the increase of immunosuppressive cells,the change of the immune function of T cell subsets and the inhibition of natural killers and lymphokine activated killer cells.In the local tumor,the infiltrating lymphocytes loss the function,and the ratio of Thl/Th2 cytokines is imbalance.

Objective To sum m arize the key points, difficulties and relevant practical experiences for ana-lyzing the scene of solved intentional hom icide cases. Methods The data of 88 solved intentional hom i-cide cases in a county from 2004 to 2013 w ere collected and the retrospective analysis w as perform ed. Results The num ber of local fem ale victim s obviously higher than non-local fem ale victim s and the num ber of non-local suspects is obviously higher than local suspects. The num ber of M ale suspects show ed higher com pared w ith the fem ale. M ost of them w ere tem porary w orkers, unem ploym ent or farm ers w ith less education backgrounds. The m ain causes of victim s’ death w ere m echanical injury or asphyxia. The m urders w ere acquaintances in m ost intentional hom icide cases. The m otive of the stranger m urders w as com m only m oney. The m urder behavior types of hom icide cases w ere related w ith people, m oney and sexual assault. C am ouflage and guilty behavior show ed the m ost significance. Conclusion The accurate identification of suspects is one of the m ost im portant task in forensic investigation and re-flects the im portance of the crim inal scene analysis for intentional hom icide cases. It also provides the direction of future research.

BACKGROUND:With elevated medical levels, limb salvage surgery for limb malignant tumor is valuable day by day. At present, the limb salvage surgery has substituted amputation and becomes mainstream and development direction of present surgical treatment of limb tumors. However, so far, there are no unified surgical indications for proximal femur tumor, which are controversial. OBJECTIVE:To investigate the efficacy of artificial total hip arthroplasty combined with extended resection of tumor sections for proximal femoral bone tumors. METHODS:Patients with proximal femoral bone tumors in the Second Xiangya Hospital of Central South University were selected and divided into control group and observation group, with 30 patients in each group. According to the disease, lesion curettage, aneurysm wal inactivation, autologous and (or) al ogeneic bone, mixed bone graft and bone graft fixation were selected to treat the control group. The observation group patients received extended resection of tumor sections and total hip arthroplasty. The operative time, intraoperative blood loss, hospital stay and joint function of two groups were compared. 2 years later, patients were revisited. Metastasis and recurrence rate, death rate and quality of life of two groups were compared. RESULTS AND CONCLUSION:There was no significant difference in intraoperative blood loss and surgery time between the two groups (P>0.05). However, hospital stay of patients in the observation group was shorter than the control group. The excellent and good rate of recovery of joint function (83%) was higher in the observation group than in the control group (53%). The metastasis and recurrence rate within 2 years after surgery (7%) and death rate (3%) were lower in the observation group than in the control group (30%, 23%). Various indicators of quality of life of patients in the observation group were significantly better than the control group (P<0.05). These results confirmed that artificial total hip arthroplasty in the treatment of proximal femur tumors is safe and effective.

In the hospital administration,the establishment and the implementation of the strategic planning of the hospital occupies a very important position.This paper,combined with the actual situation of a children's hospital in Chongqing,describes the hospital rational use of three level management system of the hospital,office,department,in order to get the full investigation of the national macro policy,the development of the medical industry and the status of the hospital and other aspects,and then supports efficient workflow to complete the hospital strategic planning.This paper also focuses on the target implementation,program implementation and project implementation of three levels,in order to illustrate the process for the implementation of the strategy to provide a reference of.hospital strategic planning formulation and implementation.