Objective To investigate the expression and significance of Slit2 in different time point after the spinal cord injury of rats.MethodSeventy adult wistar rats were randomly divided into three groups: spinal cord injury by fully transection on T10 level spinal cord (Group A); laminectomy and the spinal cord uninjuried (Group B); natural without operation (Group C).Then the rats were sacrificed and the spinal cord was taken out fresh quickly on different time-point(12h, 1, 3, 5, 7d after operation). The tissues perfusion by formaldehyde were taken out on 3, 5, 7, 14d after operation. The expressions of Slit2 were tested by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical measurement. By means of above,the author investigated the expressions and location of Slit2 after the spinal cord injury.All data were statisticsed by SPSS 11.5 software.ResultThe expression of Slit2 mRNA of appeared in the spinal cord tissue 12 hours after injury, reached peak on the 3rd day, declining gradually later. The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.ConclusionAs an important factor in axonal growth-guidance,the author crewed that Slit2 may be participated in the regeneration and rehabilitation of axons after the spinal cord injury.

Animals ; Cytotoxicity, Immunologic ; Down-Regulation ; Humans ; Influenza A Virus, H1N1 Subtype ; immunology ; physiology ; Influenza, Human ; immunology ; therapy ; virology ; Killer Cells, Natural ; immunology ; virology

Objecive To explore the curative effects of microwave endometrial ablation (MEA) for menorrhagia. Methods A total of 60 cases of menorrhagia, who had failed to respond to drug therapy and no longer desired pregnancy, were treated by MEA. Results Except for 1 case of cervical cannel adhesion, there were no other complications such as uterine perforation. Follow-up for 2 years in all the cases showed amenorrhea in 12 cases (20%), scanty menstruation in 34 cases (57%), normal menstruation in 6 cases (10%) and no improvement in 8 cases(13%), the total effective rate being 87%. The effective rate was 100% (35/35) in patients with dysfunctional uterine bleeding, 70% (14/20) in patients with endometrioma and 60% (3/5) in patients with hysteromyoma , respectively. Conclusions MEA finds a place in the treatment of menorrhagia of whatever causes. It is simple and safe, but the indications must be strictly followed for improving the curative effects.

OBJECTIVE To inquire into the risk factors of uremia patients with fungus infection and search for the effective methods to prevent and control it.METHODS All the cases of the 82 uremia patients with fungus infection in the clinic of our hospital were analyzed.RESULTS The sites of the fungus infection were intestines and stomach,blood vessel,respiratory tract,oral cavity,reproductive tract,skin soft tissue and urinary tract.The common fungus infection was Candida albicans,about 60.61 per cent.The risk factors of uremia patients with fungus infection were the old age,several underlying diseases,hypoimmunity,not using medicine properly during the treatment and invasive operation.CONCLUSIONS The effective methods to prevent and control fungus infection are as the follows: to improve the staff′s awareness of the danger of fungus infection,be strict to the rules of hospital isolation and sterilization,and be try to reduce invasive operation.We should strengthen the cleaning of the wards and the crew's hands,strengthen the monitoring of quality and pay more attention to the primary treatment.We should use the medicine properly,especially the use of antibiotics,immune suppressants and hormones,and pay attention to result of culture from the microbiological laboratory.We should cure the underlying diseases actively and improve the suffers′ immunity function.

Objective: To review the experience of using the transit time flow measurement(TTFM) in coronary artery bypass grafting(CABG). Methods: From Sept.2001 to May, 2002,90 cases underwent CABG. Among them, 40 cases with off-pump beating heart (OPCAB) and 50 with conventional coronary bypass grafting. All graft patency were verified intraoperatinlly with TTFM. The flow value of left internal mammary artery (LIMA) in 30 cases was also detected by the same way before grafting to left anterior descending (LAD). Results: There was no operative death and severe complications. The mean flow value of LIMA in 30 cases was (42.9?33.0)ml/min. The actual blood flow value, measured simultaneously by exsanguination was (37.4?28.8)ml/min. Excellent correlation was demonstrated. The mean pulsatility index value (PI) was 1.00?0.04. The flow curve showed both diastolic and systolic filling. The mean flow value of LIMA to LAD, saphenous vein graft (SVG) or radial artery (RA) to circumflex coronary artery (LCX) or diagonal (D), SVG or RA to right coronary artery (RCA) or post anterior artery (PDA) were (29.9?9.5)ml/min, (33.7?17.5)ml/min and (31.5?19.2)ml/min. The PI values were 2.47?0.88, 4.00?1.90 and 2.60?1.30, respectively. The typical flow curve was shown in all LIMA to LAD, and SVG (or RA) to LCX (or D). Blood flow filling was mainly during diastole with minimal systolic peaks during the isovolumetric ventricular contraction. SVG or RA to RCA presents a particular flow pattern characterized by a dual filling. 6 grafts were revised basing on unsatisfied flow curves, PI, and the mean flow or all of them. Significant technical error such as conduit kinking, stenosis of distal anastomosis was found. Conclusion: The patency of graft in CABG can be verified in intraoperatively by use of TTFM. The decision of checking or revising a graft can be made basing on parameters acquired from the TTFM device. To correctly analyze the parameters warrants good clinical results.

By taking Vrije University Medical Center as an example, this paper introduced the cur-rent Neuroradiologist education training system which had the characteristics of training in a comprehen-sive and focused way, multi-disciplinary integration and paying attention to the combination of academic and clinical research. Through the comparison of the status quo of China's sub specialist training, Neuro-radiologist education training system in the Netherlands provided an important reference for China's sub specialty training.

Objective To compare the clinical effect and adverse reactions of different concentrations of dezocine epidural anesthesia for cesarean section.Methods According to digital table,120 cases of elective cesarean section were randomly divided into 4 groups with 30 cases in each group, the group D1 was given with dezocine 2.5mg,the group D2 with dezocine 5mg,the group D3 with dezocine 10mg,the group M with morphine 2mg.All the drugs used in postoperative epidural analgesia after cesarean section.To observe resting pain and conduct the VAS scoring at postoperative 4,8,12,24h and record various adveme reactions.Results The VAS scores at postoperative 4,8,12h in the group D1 were lower than those in the D2,D3,M with statistical difference(all P<0.05).The VAS scores at postoperative 4,24h had no statistical difference among D2,D3,M groups(all P>0.05).The VAS scores at postoperative 8h in the group D3 were lower than those in the D2,D3,M with statistical difference(all P<0.05). There were no statistical differences among 4 groups at postoperative 24h(all P>0.05).The incidence rates of nause-a and vomiting the group M were higher than that in the group D1,D2 and D3,showing statistical difference among them(P<0.05).Conclusion Appropriate concentrations of dezocine used in postoperative epidural analgesia after cesarean section has definite effect and less adverse reactions.

3 cm ?3 cm) in 19 cases (0.8%). No nursing-related complications occurred in-hospital. Conclusion Standardized nursing management is related to the prognosis of aged ACS patients and is an important part of the perioperative preparation for primary PCI.

Objective To observe the treatment effect of Shah Mette Lo/fluticasone inhalation(Seretide)for stable stage of chronic obstructive pulmonary disease (COPD).Methods 50 patients with COPD were randomly divided into treatment group and control group.The treatment group was given Seretide (specifications of 250μg)1 -2 ceiling/time,2 times/day,after 24 weeks of treatment,the control group was given 2mg of regular salbutamol com-bined with budesonide 1mg atomization inhalation,2 times daily.The clinical symptoms were compared before and aftertreatment.Results In the treatment group,acute seizure frequency was significantly reduced,cough,wheezing singing decreased or disappeared,self -care ability improved,the control group had no obvious change.The control group:before treatment FEV1 (1.1 ±0.4)L;FEV1 % predicted value(22.5 ±5.1 )%;FEV1 /FVC%:(25.3 ±5.8)%;after treatment FEV1 (1.5 ±0.7)L;FEV1 % predicted value(29.4 ±6.2)%;FEV1 /FVC%:(32.8 ±6.6)%.The treatment group:before treatment FEV1 (1.1 ±0.6)L;FEV1 % predicted value (24.5 ±5.6)%;FEV1 /FVC%:(26.7 ±6.6)%;after treatment FEV1 (1.7 ±0.6)L;FEV1 % predicted value(33.4 ±7.8)%;FEV1 /FVC%:(37.8 ± 8.6)%.Conclusion In patients with stable COPD inhaled Seretide treatment can significantly improve lung func-tion,and it is better than sardine an alcohol combined with budesonide inhalation therapy.