OBJECTIVE:To establish a HPLC method for the determination of in vitro drug release of Baicalin liposomes.METHODS:The separation was performed on a C18 column(150mm?4.6mm,5?m)at 25℃;the mobile phase was composed of methanol-water-phosohoric acid(47∶53∶0.2)at a flow rate of 1.0mL?min-1.The ultraviolet wavelength was set at 280nm and the sample size was 10?L.RESULTS:The calibration curve showed good linearity in the range of 1.01～80.80?g?mL-1(r=0.999 8)and the average recovery rate was 100.05%(RSD=0.93%).CONCLUSION:The method is sensitive,simple and accurate,and applicable for the study of in vitro drug release of Baicalin liposomes.

To investigate the common mutation patterns of hereditary nonpolyposis colorectal cancer(HNPCC) genes.MethodsUsing PCR-agarose electrophoresis and PCR-SSCP to screen the hMSH2, hMLH1 genes mutation of 9 HNPCC patients and 4 family members. ResultsAbnormal shifting of PCR productions was identified in 7 patients and 2 HNPCC family members out of 9 different families. ConclusionPCR-agarose and PCR-SSCP are simple, rapid , sensitive, economic and specific methods that can be used to detect HNPCC gene hMSH2, hMLH1 mutations.

After the concept of damage control resuscitation is introduced, in the most severely injured casualties, when the lethal triad of hypothennia, acidosis, and coagulopathy are present, resuscitation prac-tice for damage control usually focuses on rapid reversal of acidosis and prevention of hypothermia, while di-rect treatment of eoagulopathy has been relatively neglected. Recent studies have brought back to light the importance of treating the coagulopathy of trauma at an earlier stage. This review focused on the importance and the right time of treating this disorder, analyzed the methods of addressing the early coagalopathy in damage control resuscitation and gave some advices.

A cochlear implant (CI) is a kind of surgically implanted electronic device that provides a sense of sound to a person who has severe to profound sensorineural hearing impairment. However, studies have shown that the performance in music perception for CI users has not been achieved to an ideal level. It is important to evaluate the ability to perceive music using well designed music perception test materials, in order to improve the quality of life for cochlear implant users. This paper reviewed the studies on assessing music perception ability for adult cochlear implant users from the existing literature.
Cochlear Implantation ; Cochlear Implants ; Humans ; Music ; Pitch Perception

[Aim] Discuss Chen's treatment effect of infants viral myocarditis ayyhythmia. [Methods] He made suit medicine on two cases and analysed the disease'classification and main clinical manifestation and relevant therapy and his empirical formula, according to some factors ,such as the etiology and pathogens, course of disease and so on. [Results] (1)For viral myocarditis early disease caused by tachyarrhythmia, Mr. Chen advocated using the methods to treat which is clearing away heat and toxin and calming the pulse, the prescription used is Qingxinjiedu Decoction;however, those ones with il ness are offered a way of suppiementing qi and nourishing yin and warming the heart yang in order to remove pulse frequency, Chen used the modified YiQiShengMai Decoction for treatment.(2) The professor Chen paid attention to clearing hot and humid and supplementing qi and warming yang to cure slow arrhythmia due to acute viral myocarditis,the modified Huolian Decoction is used for this disease; For the people with a long il ness delayed healing, Chen worked a Decoction by himself which is Wenyangfumai Decoction,so that it could supplement qi and warm yang and invigorate the circulation of blood through pulse; For the slow arrhythmia appeared to sequela period, Chen thought that treatment should be insisted on promoting blood circulation to remove blood stasis and warming and activating the heart yang, the prescription used is Tongmai oyal liquid.[Conclusion]Professor Chen had gained a satisfied effect to treat infants viral myocarditis arrhythmia with the methods of clearing away heat and toxin, supplementing qi and nourishing yin and warming the heart yang and removing pulse frequency from the different stages and types.

Audiology ; methods ; Hearing Tests ; methods ; Humans ; Time Factors

Objective To establish musculoskeletal model and finite element model of kidney and blood stasis type knee osteoarthritis (KOA),and to analyze the cartilage stress characteristics of kidney and blood stasis type KOA.Methods Data from knee CT,MRI of kidney and blood stasis type KOA volunteers was used to construct musculoskeletal model and finite element model based on AnyBody and ANSYS software.Surface electromyogram (sEMG) verification and mutual verification were conducted.KOA bone stress and strain parameters were observed at the moving angles of 0°,-40°,-90°,-180° of the KOA musculoskeletal model,in order to explore the cartilage stress characteristics.Results When the positions of kidney and blood stasis type KOA patients varied from standing to squatting,the knee cartilage surface stress revealed a nonlinear increasing trend.Kidney and blood stasis KOA patient's knee cartilage stress near the distal end was significant different from that of normal subjects (P＜0.05),while the KOA patient's knee cartilage stress near the distal end and proximal front side had no significant differences with that of normal subjects (P＞0.05).Conclusions For kidney and blood stasis type of patients with KOA,cartilage surface stress displays a nonlinear increasing trend along with the stress concentration at the motion cartilage surface,which is consistent with the clinical features.

Objective:To design a tuberculosis(TB) laboratory equipment management system based on Internet of Things (IOT), and put forward the specific plan of IOT technology used in TB laboratory equipment management.Methods: Bases on data acquisition technology, wireless mobile terminal based on wireless LAN technology, radio frequency identification (RFID) technology and computer technology, the Web application system architecture built equipment management system to realize internal information sharing and management of laboratory equipment.Results: The tuberculosis laboratory equipment management system using RFID networking technology and 2-dimensional bar code identification based on recognition, positioning and tracking of equipment, built system architecture by the user login module, device management module, equipment ledger management module and public affairs workflow management module. It has high efficiency, and is convenient for data query, updating and unifying management, information monitoring, real-time dynamic effect of maintenance equipment for positioning and bio safety management.Conclusion:The application of program links TB laboratory equipment management system networking and related technology based on IOT can achieve recognition, positioning and tracking of laboratory equipment, and also can effectively improve the overall level of laboratory equipment management intelligence and laboratory management efficiency.

After the status quo of ESI-covered pharmacology, toxicology and clinical medicine in domestic universities of traditional Chinese medicine was described, the papers and highly-cited papers published by these universities, total citations, citations of each paper were ranked and compared.The related problems and development tendency of different subjects were analyzed with suggestions put forward for the construction of first class subjects in domestic colleges and universities of traditional Chinese medicine.

Objective To investigate the changes of waist muscle density and area in the subjects with spiral CT scan examination,and through the quantitative analysis of different age and gender to get the degree of lumbar muscle degeneration.Methods 240 cases of lumbar CT scan reconstruction images (including the patients suffering from low back pain)were collected.According to the age,20 - 29 years old group,30 - 39 years old group,40 -49 years old group,50-59 years old group,60-69 years old group,and 70-79 years old group were set up.The lumbar transverse psoas muscle,lumbar side muscle,erector spinae cross-sectional areas (CSA)and mean CT values were measured at different levels.Scanning equipment:Toshiba Activion 16-slice spiral CT scanners, acquisition range from the waist 1 to S1.Results ①Compared between the measurement results of the same age groups (except 70-79 years old group),the average CSA of the male at the same level waist was higher than the female (P <0.05).②Compared between the same level groups of 20-29 years,30-39 years and 40-49 years, there were no significant differences (P >0.05).Compared between the same level groups of 50-59 years,60-69 years and 70-79 years,there was no significant difference (P >0.05).③Compared the CSA of man’s erector spinae muscle between 40-49 years group and 50-59 years group,there were significant differences (P <0.05).④Compared the same level CSA of female between 20-29 years,30-39 years,40-49 years,50-59 years,60-69 years,70-79 years groups,and the adjacent groups,there were no significant differences (P >0.05).⑤The same level average density of erector spinae muscle was lowest compared with the psoas major muscle and quadratus lumborum muscle,and falling faster,the psoas major muscle was relatively higher.The same level average density of female’s erector spinae muscle was lower than man’s,specially in 70-79 years group.Conclusion Multi-slice spiral CT can be used to diagnose the lumbar degenerative conditions.