Objective: To design a upper computer software which can achieve data acquisition, display, motion control for sickbed-control test system. Methods: In Visual C++6.0 environment, take advantage of Advantech's development kit, and integrate multi-thread and dual-buffer technology to achieve. PC translate user's intentions into control commands, then sent commands to PCI1240, PCI1716 through the PCI interface, PCI1240 drive stepper motor to control the movement of the bed, while PCI1716 collect movement state information. Results: Movement can be stopped immediately by clicking the stop button even during the reciprocating motion, and solve screen flicker when drawing the real-time curves. The software has been test in bed-control system many times and achieved good results. Conclusions: This paper's method realized the sickbed's motion control, data acquisition, data storage and display, compared with the method that using single chip machine and general electromotor, our method makes bed movement more precise and smooth, more function are achieved, and the software has been successfully used in the sickbed-control system.

Objective To evaluate the clinical curative effect of Liuwei Nengxiao capsules combined with acupoint application for functional constipation. Methods According to the order of treatment, 105 patients with functional constipation were divided into combined treatment group, Liuwei Nengxiao group and acupoint application group with random number table, 35 cases in each group. Combined treatment group was treated with both Liuwei Nengxiao capsules orally taking and Tongbian Fang acupoint application in Shenque. Liuwei Nengxiao group was treated with Liuwei Nengxiao capsules alone, and acupoint application group was treated with Tongbian Fang acupoint application in Shenque alone. The course of treatment lasted for 10 days. The symptom scores were assessed before and after treatment, to evaluate the efficacy. Results The symptom scores of the three groups all decreased after treatment (P<0.05). The total effective rates of combined treatment group, Liuwei Nengxiao group and acupoint application group were 100%(35/35), 94.3% (33/35) and 88.6% (31/35), respectively, and the clinical effect of combined treatment group was better than that of other two groups (P<0.01). Conclusion The clinical effect of Liuwei Nengxiao capsules combined with acupoint application in treating functional constipation is superior to that of separate applications.

Objective To study the risk factors of central lymph node (CLN) metastasis in papillary thyroid carcinoma (PTC),to provide the basis for clinical treatment.Methods A total of 407 patients with PTC in thyroid surgery,the Third Affiliated Hospital of Harbin Medical University from January 2010 to June 2012 who had undergone at least ipsilateral CLN were studied.These factors included normal situation,thyroidglobulin antibody or thyroid microsomal antibody,family history,pathological features (the size of primary,tumor multifocal,tumor location and capsular invasion),TNM staging and CLN metastasis.Univariate analysis and multivariate analysis were performed using x2 test and binary Logistic regression test,respectively.Results The CLN metastasis rate was 51.351％ (209/407) of the 407 patients with PTC.The CLN metastasis was significantly associated with age,tumor multifocal,the size of primary tumor,capsular invasion and TNM (x2 =21.080,14.974,47.671,12.858,8.765,all P ＜ 0.01).Sex,thyroid globulin antibody or thyroid microsomal antibody,family history and tumor location were not associated with CLN metastasis (x2 =1.457,1.106,0.000,0.260,all P ＞ 0.05).Multivariate analysis indicated that age (＜ 45 year),the size of primary tumor (≥ 1.0 cm),tumor-muhifocal,and TNM (T3 + T4) were the independent risk factors for CLN metastasis [odds ratio (OR) =0.937,2.347,0.380,0.389,all P ＜ 0.01].Conclusions Age (＜ 45 year),the size of primary tumor (≥ 1.0 cm),tumor-multifocal and TNM (T3 + T4) are risk factors for CLN metastasis in PTC.Dissection of CLN should be considered for PTC patients with these factors.

Cardiovascular Diseases ; diagnosis ; genetics ; Humans ; MicroRNAs ; blood ; genetics

Objective To analyze death causes in patients with acute myocardial infarction complicated by cardiac rupture.Methods The patients who were admitted with cardiac rupture after acute myocardial infarction in Beijing Anzhen Hospital Affiliated to Capital Medical University from January 2012 to December 2014 were enrolled.These patients were divided into death group and survival group.Then statistical analysis was performed for the clinical data of these patients in two groups,Logistic regression analysis was used to analyze the risk factors with statistical significance.Results A total of 59 patients diagnosed cardiac rupture after acute myocardial infarction were enrolled in this study,including 50 patients died,and 9 patients survived.There were significant differences between the two groups of patients with clinical baseline data.Compared with the survival group,death group had higher age ((69.94 ± 9.24) years old vs.(61.56 ± 9.14) years old,t =2.511,P =0.015),the higher incidence of malignant arrhythmia (22.0％ vs.0,x2 =4.070,P =0.044),the lower proportion of alcohol abuse (12.0％ vs.44.4％,x2 =5.704,P =0.017),higher fasting blood glucose ((8.97 ±3.98) mmol/L vs.(6.06± 1.25) mmol/L,t =4.153,P＜0.01) and triglycerides ((1.78±0.50) mmol/L vs.(1.39±0.20) mmol/L,t =4.146,P＜0.01),higher proportion of pulmonary arterial hypertension(22.0％ vs.0,x2 =4.070,P =0.044) and brain natriuretic peptide (406.50 (110.00,570.28) ng/L vs.33.00 (20.00,176.00) ng/L,P=0.004),smaller the left ventricular end diastolic diameter((47.76±5.13) mm vs.(52.22 ±4.66) mm,t =-2.434,P =0.018).The use of fondaparinux sodium (26.0％ vs.0,x2 =4.920,P =0.027),heparin (48.0％ vs.88.9％,x2 =5.138,P =0.023) and nitrates (72.0％ vs.100.0％,x2 =5.361,P =0.021)were significantly differences.The occurrence of acute heart failure in patients in death group was significantly higher than the survival group (11.0％ vs.0,x2 =3.258,P =0.071),but the difference was not significant.Logistic regression analysis showed that old age (OR =1.151),fasting blood glucose (OR =1.974)and heart rupture were significandy correlated (P＜ 0.05).Conclusion Cardiac rupture patients have a high mortality rate after myocardial infarction.Advanced age and fasting blood glucose were risk factors,while the use of common heparin is protective factor.Patients should be evaluated in a timely manner to assess the prognosis and to take targeted measures.

Anthrax is a malignant infectious disease caused by Bacillus anthracis spores,after entering the host Bacillus an-thracis produces and releases anthrax toxin,which is the main cause leading to death of the host. The anthrax toxin is composed of two enzymatically active components:lethal factor(LF)and edema factor(EF),and one shared receptor binding and translocation com-ponent:protective antigen(PA). PA combined with LF is called lethal toxin(LeTx),while PA combined with EF called edema toxin (EdTx). Currently,the main drugs for treating anthrax are antibiotics,but antibiotics can only kill part of anthrax spores and bacte-ria,and cannot inhibit the activity of anthrax toxin. So it is necessary to develop novel drugs for inhibiting anthrax toxin. This review summarizes the evolution of small-molecule inhibitors of anthrax toxin respectively targeting PA,LF and EF.

Objective To investigate effect serum Bcl-2 and Bax in patients with myoma of uterus by Jiang Sanqi.Methods 92 patients with uterine fibroids were selected,and randomly divided into experiment group and control group.Control group of 46 cases treated with goserelin once 3.6 mg every month,subcutaneous injection,mifepristone 12.5 mg,once a day for oral treatment.Experiment group of 46 cases treated with Jiang Sanqi,0.5 g/kg,once a day for boiling water, for 3 months.The level of Bcl-2 and Bax in serum, clinical efficiency, diameter of tumor and complications were compared after treatment.Results The levels of Bcl-2 of experimental group patients was lower than control group, the level of Bax was higher than that of the control group (P<0.05).The total efficiency of experimental group(89.13%)was higher than that of the control group(71.74)%(P<0.05). The experimental group tumor diameter was smaller than the control group(P<0.05).Complications of the experiment was lower than the control group (P<0.05).Conclusion Jiang Sanqi can significantly reduce the serum Bcl-2 of patients with uterine fibroids,increase the expression level of Bax, reduce the uterine fibroids diameter effect, reduce the incidence rate of hormone suppression of drug side effects, has a great significance in clinical.

Objective To evaluate the role of Toll-like receptor 4 (TLR4) in mitigation of inflammatory responses following myocardial ischemia-reperfusion (I/R) injury by sufentanil postconditioning in rats.Methods Thirty Wistar rats were randomly divided into 3 groups (n =10 each):sham operation group (S group); I/R group; sufentanil postconditioning group (SP group).Myocardial I/R was produced by temporary ligation of left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion.In group SP,sufentanil 0.6 μg/kg was injected intravenously at 5 min before reperfusion.The myocardial specimens and blood samples were taken at the end of 120 min reperfusion for determination of the interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations and TLR4 content (by ELISA).Results Compared with S group,the levels of TLR4,IL-6 and TNF-α were significantly increased in the other two groups.Comparedwith I/R group,the levels of TLR4,IL-6 and TNF-α were significantly decreased in group SP.Conclusion The mechanism by which sufentanil postconditioning mitigates inflammatory responses following myocardial I/R injury may be related to downregulation of TLR4 expression in myocardial tissues of rats.

Objective To compare the effects and prognosis of craniotomy evacuation of hematoma and hard tunnel minimally invasive perforation for hypertensive basal ganglia hemorrhage ( HBGH ) . Methods A total of 192 patients with HBGH from January 2005 to December 2013 were retrospectively reviewed , including 96 cases of craniotomy evacuation of hematoma and 96 cases of minimally invasive puncture drainage .No significant differences were found in age , gender, lesion side,and Glasgow coma scale between the two groups ( P >0.05 ).The intraoperative bleeding volume , postoperative residual hematoma , operation time, hospitalization duration , and the postoperative clinical curative effects at 3 weeks and 6 months after operation were compared between the two groups . Results As compared with the craniotomy group , the operation time of the hard tunnel minimally invasive perforation group was significantly shorter [31 (24 -39) min vs.152 (131 -170) min, Z =-11.975, P =0.000], the intraoperative blood loss was significantly less [0 ml in 30 cases and <5 ml in 66 cases vs.(332.4 ±20.2) ml],and the hospitalization duration was significantly shorter [14 (10-17) d vs.64 (44-75) d, Z=-44.217, P=0.000].However, the postoperative residual hematoma was larger in the hard tunnel minimally invasive perforation group than in the craniotomy group [13 (8-17) ml vs.7 (4-12) ml, Z=-11.573, P=0.000].At the third week of postoperation , the Glasgow Outcome Scale in the hard tunnel minimally invasive perforation group was higher [4 (2-4) vs.3 (1-4), Z=-8.215, P=0.000], and the Barthel Index was higher [(59.9 ±4.5) vs.(54.9 ±4.9), t=7.370, P=0.000] than the craniotomy group.At sixth month postoperation, the Glasgow outcome scale in the hard tunnel minimally invasive perforation group was higher [3 (2-4) vs.2 (1-4), Z=-7.448, P=0.000], and the Barthel Index was higher [(64.3 ±8.4) vs.(59.7 ±6.8), t=4.156, P=0.000].No significant differences in mortality were found between the two groups (P >0.05). Conclusion Minimally invasive hard tunnel puncture drainage is significantly superior to craniotomy evacuation of hematoma in the treatment of HBGH and deserves to be promoted for clinical performance .

Objective This study aimed at assessing the long-term efficacy of EVL for esophageal variceal bleeding. Methods Long-term EVL was assessed in 263 patients with variceal bleeding,among them 71 were of Child- Pugh Class A,82 of Class B and 110 of Class C.EVL was repeated at two week intervals until varices were eradicated.Results Esophageal varices were eradicated in 91% of the 238 patients who survived for more than three months after 1 to 10 (average 4) successive ligations.Recurrent variceal bleeding occurred in 15% of the 263 patients and was markedly reduced after eradication of the varices.The overall cumulative survival rates at 1,3,and 5 years were 76%,62%,and 57%,respectively.The prognosis was influenced by Child- Pugh's risk grade.56 of the 263 patients died during the study period,and the major cause of death was liver failure.Conclusion EVL is effective for long-term control of esophageal variceal bleeding.Repeated EVL re- duces rebleeding rate and improves survival in patients who have bled from esophageal varices.