Objective To evaluate the clinical curative effect of Liuwei Nengxiao capsules combined with acupoint application for functional constipation. Methods According to the order of treatment, 105 patients with functional constipation were divided into combined treatment group, Liuwei Nengxiao group and acupoint application group with random number table, 35 cases in each group. Combined treatment group was treated with both Liuwei Nengxiao capsules orally taking and Tongbian Fang acupoint application in Shenque. Liuwei Nengxiao group was treated with Liuwei Nengxiao capsules alone, and acupoint application group was treated with Tongbian Fang acupoint application in Shenque alone. The course of treatment lasted for 10 days. The symptom scores were assessed before and after treatment, to evaluate the efficacy. Results The symptom scores of the three groups all decreased after treatment (P<0.05). The total effective rates of combined treatment group, Liuwei Nengxiao group and acupoint application group were 100%(35/35), 94.3% (33/35) and 88.6% (31/35), respectively, and the clinical effect of combined treatment group was better than that of other two groups (P<0.01). Conclusion The clinical effect of Liuwei Nengxiao capsules combined with acupoint application in treating functional constipation is superior to that of separate applications.

Objective: To design a upper computer software which can achieve data acquisition, display, motion control for sickbed-control test system. Methods: In Visual C++6.0 environment, take advantage of Advantech's development kit, and integrate multi-thread and dual-buffer technology to achieve. PC translate user's intentions into control commands, then sent commands to PCI1240, PCI1716 through the PCI interface, PCI1240 drive stepper motor to control the movement of the bed, while PCI1716 collect movement state information. Results: Movement can be stopped immediately by clicking the stop button even during the reciprocating motion, and solve screen flicker when drawing the real-time curves. The software has been test in bed-control system many times and achieved good results. Conclusions: This paper's method realized the sickbed's motion control, data acquisition, data storage and display, compared with the method that using single chip machine and general electromotor, our method makes bed movement more precise and smooth, more function are achieved, and the software has been successfully used in the sickbed-control system.

Cardiovascular Diseases ; diagnosis ; genetics ; Humans ; MicroRNAs ; blood ; genetics

Adult ; Aged ; Bronchoalveolar Lavage Fluid ; chemistry ; cytology ; Chemokine CCL3 ; metabolism ; Humans ; Macrophage Migration-Inhibitory Factors ; metabolism ; Macrophages ; metabolism ; pathology ; Male ; Middle Aged ; Silicosis ; metabolism ; pathology

With the development of society and the improvement of people's living standards, the effect of Chinese medicine in treatment and health care is more and more prominent. The herbal decoction pieces are the important part of Chinese medicine,it can be applied directly to clinical treatment and it's also the raw material of Chinese patent medicine. Therefore, the quality of herbal decoction pieces is quite important. The parts of the production of herbal decoction pieces are numerous, and there are possibilities of adverse effects on the quality of the herbal decoction pieces in every part. In this paper, we based on the production chain of herbal decoction pieces, analyzed the main problem that affect the quality of herbal decoction pieces in the part of selection of Chinese herbal medicines, planting, purchasing, processing, packaging, storage and transport, such as the poor quality of seed and seedlings of plant-based Chinese medicines, some plants left their place of origin and have been introduced in the place that is not suitable for this kind of plant, the insufficient growth time and the excessive harmful substances. The purchasers and the accepters lack of professional knowledge and professional ethics. The mechanism of processing is not clear, the standards can not be uniformed, and lack of qualified person in processing, etc. So we suggest: intensify the basic research of key scientific issues. Improve the quality of persons who work in herbal decoction pieces; Establish an "integration" mode of operation in herbal decoction pieces enterprise; Breeding high quality plant resources, establish the large-scale planting basement; Make the packing of herbal decoction pieces standard; Establish the modernization traditional Chinese medicine logistics enterprise.
Chemistry, Pharmaceutical ; economics ; manpower ; standards ; Drug Packaging ; economics ; manpower ; standards ; Drug Storage ; economics ; standards ; Drugs, Chinese Herbal ; chemistry ; standards ; Humans ; Medicine, Chinese Traditional ; economics ; standards ; Quality Control

Objective To determine whether the anti-proliferation effect of L-arginine is due to inhibiting the expression of cyclin dependent kinase-2 (CDK2)、 CyclinE and proliferation cell nuclear antigen (PCNA) in blood vessel after balloon injury. Methods Rats were randomized into three groups: Group S (sham operation group), Group C (balloon injury control group) and Group L (balloon injury + L-arginine group). After 14 days, blood samples were collected for biochemical studies, and the thoracic aortas were harvested for immunohistochemistry. The expression of CDK2、 CyclinE and PCNA were measured by means of computer image analayzer. Results The levels of plasma nitric oxide (NO) in group C were significantly lower than those in group S. Compared with group C, the levels of plasma NO increased (P

Objective Investigate the relation between decrease of apoptosis caused by delayed preconditioning and expression of SMAC and XIAP.Methods Sprage-Dawleyt rats were divided into four groups: control,sham,I/R and IPC/SWOP.The rats in I/R group underwent ischemia for 1 hour by classic artery ligation and reperfusion for 1 hour.The rats in IPC/SWOP group underwent tree cycles of 5-minute ischemia and 5-minute reperfusion 24 hours prior to the index occlusion.Cell apoptosis was measured by flow cytometry,the activity of caspase-3 was also measured.The expression of SMAC and XIAP in cytosol of myocardial cell was measured by Western blot.Results Cell apoptosis rate,activity of caspase-3 and expression of SMAC significantly increased in I/R as compared with control(P

Objective To establish the quality standard for Xiaozhong Tablets (Radix Angelicae Sinensis, Pheretima, etc.). Methods TLC was performed to Radix Angelicae Sinensis, Pheretima, Ursolic acid. The content of Aconitine was identified by TLC. HPLC was used to determine Strychnine content. Results The characteristic for identification by TLC was distinct and hightly specific. The quantification method of Strychnine had the linear range of 0.52～1.56 ?g (r =0.9998). The average recovery was 99.9% (RSD=1.77%, n =5). Conclusion The methods of identification and quantification were accurate, realizable and reproducible. It can be used effectively for the quality control of Xiaozhong Tablets.

Objective To evaluate the role of Toll-like receptor 4 (TLR4) in mitigation of inflammatory responses following myocardial ischemia-reperfusion (I/R) injury by sufentanil postconditioning in rats.Methods Thirty Wistar rats were randomly divided into 3 groups (n =10 each):sham operation group (S group); I/R group; sufentanil postconditioning group (SP group).Myocardial I/R was produced by temporary ligation of left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion.In group SP,sufentanil 0.6 μg/kg was injected intravenously at 5 min before reperfusion.The myocardial specimens and blood samples were taken at the end of 120 min reperfusion for determination of the interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations and TLR4 content (by ELISA).Results Compared with S group,the levels of TLR4,IL-6 and TNF-α were significantly increased in the other two groups.Comparedwith I/R group,the levels of TLR4,IL-6 and TNF-α were significantly decreased in group SP.Conclusion The mechanism by which sufentanil postconditioning mitigates inflammatory responses following myocardial I/R injury may be related to downregulation of TLR4 expression in myocardial tissues of rats.

Objective To improve the level of diagnosis and treatment of acute appendicitis in leukemia.Method 17 cases of acute appendicitis in leukemia that were treated from 1980 to 2002 were reviewed.Result All 17 cases underwent appendectomy,16 survived the immediate postoperative period,only one died.Conclusions Leukemia were predisposed to acute appendicitis than normal during chemotherapy period and our experience supports the surgical management of acute appendicitis in leukemia.Because immunosuppression and thrombocytopenia were the main causes of additional surgical dangers,relative methods should be applied in perioperation period.The complications of incision were common,so the observation of incision should be emphasized.