Objective: To design a upper computer software which can achieve data acquisition, display, motion control for sickbed-control test system. Methods: In Visual C++6.0 environment, take advantage of Advantech's development kit, and integrate multi-thread and dual-buffer technology to achieve. PC translate user's intentions into control commands, then sent commands to PCI1240, PCI1716 through the PCI interface, PCI1240 drive stepper motor to control the movement of the bed, while PCI1716 collect movement state information. Results: Movement can be stopped immediately by clicking the stop button even during the reciprocating motion, and solve screen flicker when drawing the real-time curves. The software has been test in bed-control system many times and achieved good results. Conclusions: This paper's method realized the sickbed's motion control, data acquisition, data storage and display, compared with the method that using single chip machine and general electromotor, our method makes bed movement more precise and smooth, more function are achieved, and the software has been successfully used in the sickbed-control system.

Objective To evaluate the clinical curative effect of Liuwei Nengxiao capsules combined with acupoint application for functional constipation. Methods According to the order of treatment, 105 patients with functional constipation were divided into combined treatment group, Liuwei Nengxiao group and acupoint application group with random number table, 35 cases in each group. Combined treatment group was treated with both Liuwei Nengxiao capsules orally taking and Tongbian Fang acupoint application in Shenque. Liuwei Nengxiao group was treated with Liuwei Nengxiao capsules alone, and acupoint application group was treated with Tongbian Fang acupoint application in Shenque alone. The course of treatment lasted for 10 days. The symptom scores were assessed before and after treatment, to evaluate the efficacy. Results The symptom scores of the three groups all decreased after treatment (P<0.05). The total effective rates of combined treatment group, Liuwei Nengxiao group and acupoint application group were 100%(35/35), 94.3% (33/35) and 88.6% (31/35), respectively, and the clinical effect of combined treatment group was better than that of other two groups (P<0.01). Conclusion The clinical effect of Liuwei Nengxiao capsules combined with acupoint application in treating functional constipation is superior to that of separate applications.

Objective To study the effects of Celastrus orbiculatus extracts (COE) on the expression of VEGF and CECs of Hepal-6 tumor bearing mice.Methods Animal Models of Hepal-6 portability liver tumor bearing mice were established and randomly assigned to six groups (eight mice per group) as follows:untreated control group,solvent vehicle control (DMSO) group,physiological saline control group,cisplatin-treated group,and different dosages COE-treated groups (40 mg/kg,20 mg/kg,10 mg/kg).To approach the effects of COE on the expression of VEGF and CECs in Hepal-6 toumor bearing mice.Results The ELISA and flow cytometer showed that COE could significantly inhibit the expression of VEGF(45.24±21.36) pg/ml and CECs (0.83 ± 0.10) ％ compared with the physiological saline control group (P＜ 0.01 ) with the dose of 20mg/kg.Conclusion COE could significantly down-regulate the expression of VEGF and CECs.It may be related with the effect of COE in inhibiting angiogenesis of tumor.

ObjectiveTo explore the effects of splenectomy on hepatic fibrosis and on the expression of PDGF-B in the liver and PDGF-BB in the serum of rats with hepatic fibrosis. MethodsBy hypodermic injection CCl4, we established 65 rat models with hepatic fibrosis, splenectomies were performed in the three groups at different phases: before hypodermic injection CCl4 (A group), five weeks after hypodermic injection CCl4 (B group), and ten weeks hypodermic injection CCl4 (C group). The control groups were established at the same time, with samples of the livers and serum of the rats taken in different phases. The expressions of PDGF in the liver were detected by immunohistochemistry technique and the degree of hepatic fibrosis was detected by HE staining. The serum levels of PDGF-BB were analyzed by ELISA technique. ResultsAbsorbance values of PDGF-B in the experimental group were significantly lower than the control groups (P＜0. 05). Serum levels of PDGF-BB of the rats after splenectomy were significantly lower than those in the control groups (P＜0.05). HE and Masson's staining showed that the progression of Hepatic fibrosis was slow in the A group. Hepatic pathologic state was significantly relieved in the B group and the inflammation and fibrosis was relieved in the C group. Conclusion Earlier period splenectomy could delay the proceeding of experimental hepatic fibrosis. After splenectomy the decline in the level of PDGF may be one of the mechanisms causing the delay.

Objective: To investigate the undertected situation in gonococci culture of male urethral secretions. Methods: The 362 cases of male ureethral secreations were simultaneously detected with fluorescence quantitative PCR, germiculture and direct smear method. The results in three detections were statistically analyzed. Results: The fluorescence quantitative PCR positive rate was 29.56%, near to direct smear method (26.52%)（x2=0.83，P>0.05）; the germiculture positive rate(4.42%) was significantly lower than the positive rates of fluorescence quantitative PCR(x2=81.10,P<0.01) and direct smear method(x2=67.60,P<0.01). Conclusion The simple gonococci culture has high missed detection rate(>80%) in detecting male cases. Ensuring the quality of samples before analysis and detecting with two methods or above are the effective measures to improve the gonococci detection rate.

Objective To investigate the vascular protective effect of pretreatment with candesartan on cerebral ischemia in rats.Methods Forty-eight male Sprague Dawley rats were randomly divided into sham operation,ischemia-reperfusion,low-dose candesartan group was randomly redivided into reperfusion 24 h and 72 h subgroups (n = 6).A model of middle cerebral artery occlusion was induced by intraluminal suture method after 4 weeks of drug gavage.Blood pressure was measured preoperatively.The neurological scores were performed after 24 and 72 hours of reperfusion.Then the rats were decapitated and the brains were removed.The infarct volume was measured using 2,3,5-triphenyltetrazolium chloride staining.The expression of vascular endothelial growth factor (VEGF) protein in ischemic regions was detected by immunohistochemical staining and Western blot analysis.Results The neurological scores of the low-dose and high-dose candesartan groups at 24 and 72 hours after reperfusion were significantly better than those of the ischemia-reperfusion group (P = 0.008and 0.001,respectively),and the infarct volume was reduced significantly (P=0.010 and 0.000,respectively).At the second week after medication,the blood pressure was decreased significantly in the high-dose candesartan group,and there was no significant antihypertensive effect in the low-dose candesartan group.The positive expression of VEGF mainly distributed in the vascular endothelial cells around the infarcts.Its expression was further upregulated with the time.It reached the peak after 72-hour reperfusion.The result of Western blot analysis was consistent with that of immunohistochemical staining.Conclusions Candesartan may reduce the infarct volume by upregulating the expression of VEGF in the ischemic region and improve the neurological score.

Objective To determine whether the anti-proliferation effect of L-arginine is due to inhibiting the expression of cyclin dependent kinase-2 (CDK2)、 CyclinE and proliferation cell nuclear antigen (PCNA) in blood vessel after balloon injury. Methods Rats were randomized into three groups: Group S (sham operation group), Group C (balloon injury control group) and Group L (balloon injury + L-arginine group). After 14 days, blood samples were collected for biochemical studies, and the thoracic aortas were harvested for immunohistochemistry. The expression of CDK2、 CyclinE and PCNA were measured by means of computer image analayzer. Results The levels of plasma nitric oxide (NO) in group C were significantly lower than those in group S. Compared with group C, the levels of plasma NO increased (P

Objective To establish the quality standard for Xiaozhong Tablets (Radix Angelicae Sinensis, Pheretima, etc.). Methods TLC was performed to Radix Angelicae Sinensis, Pheretima, Ursolic acid. The content of Aconitine was identified by TLC. HPLC was used to determine Strychnine content. Results The characteristic for identification by TLC was distinct and hightly specific. The quantification method of Strychnine had the linear range of 0.52～1.56 ?g (r =0.9998). The average recovery was 99.9% (RSD=1.77%, n =5). Conclusion The methods of identification and quantification were accurate, realizable and reproducible. It can be used effectively for the quality control of Xiaozhong Tablets.

ObjectiveTo study the characteristics of nonfunctioning parathyroid cysts (NFPTC) for its diagnosis and treatment.Methods 5 cases of NFPTC were hospitalized during 1985～1999. The clinical features, serum calcium evaluation ultrasonography and nuclide scanning were performed. Postoperative pathologic and immunihistochemistry detection in NFPTC were made.ResultsTumours in the 5 cases were removed by operation and diagnosis was verified by pathology. Tumors were all positive for chromogranin A (CgA),synaptophysin(Syn),and neuron specific enolase(NSE). Follow up found no recurrence. ConclusionsSurgical resection is most effective in treating NFPTC and diagnosis is made by pathologic examination.