The internet addiction disorder is an important problem under the internet circumstance.Improving self-regulated ability is a good method to avoid the problem.The article uses the selfregulated circle theory to gain the self-regulating strategy in three phases:forethought,performance or volitional control,self-reflection.These strategies include making goal,planning strategy,self-observation and self-record,attribution.

Objective To screen enterohemorrhagic Escherichia coli(EHEC) strain and declare it as a standard strain of China Medical Bacterial Species Conservation and Management Center(CMCC).On the base,to prepare strain reference and genomic DNA reference of EHECand declare them as national drug reference with independent intellectual property rights in our country.Methods According to GB4789.6-2016 National Food Safety Standards-Food Microbiology TestDiarrheagenic Escherichia coil Test,the EHEC strain was screened from 160 Escherichia coli strains from patients with diarrhea and declared as a standard strain of CMCC according to management regulations.EHEC bacterial solution and genomic DNA solution were prepared,and freeze-drying technology was used to prepare 600 strain(10~3 CFU/sample) and genomic DNA(20 ng/sample) samples respectively.20 strain and 20 genomic DNA samples were randomly selected for uniformity test.Samples storing at 25 ℃ and 37 ℃ for 1,3,5 and 7 d were taken respectively to test the transportation stability.Then the samples were tested for the short-term storage stability by storing at 4 ℃ for 7,14 and 28 d,and for the long-term storage stability by storing at 20 ℃ for 14,28 and 60 d.Three laboratories were organized for collaborative calibration.20 food products were chosen as the substrate to evaluate the application effect of strain samples.Results The one EHEC strain selected from 160 Escherichia coli strains from patients was finally declared as the CMCC(B) 43207standard strain.In the uniformity test,F_(strainsample)=0.662 0.05,and eae,stxl and stx2 of 20genomic samples were all positive.After storage at 25 and 37 ℃ for 7 d,-20 ℃ for 60 d and 4 ℃ for 28 d,the viable bacteria content of the strain samples was still 103 CFU/sample,and eae,stxl and stx2 of the genomic samples were positive.EHEC strains and genomic DNA samples selected randomly were identified as EHEC by three laboratories,the viable bacteria content was 10~3 CFU/sample,and eae, stxl and stx2 were all positive.It was detected in 20 kinds of food substrates after adding samples,but not in the background control.EHEC strain and the genomic DNA sample were included in drug standard materials in our country,numbered 80024 and 80056,respectively.Conclusion The prepared EHEC strain and genomic DNA standard materials with independent intellectual property rights can improve the timeliness of EHEC testing and make up for the gap in our country.

Anoikis is a special programmed cell death and plays important roles in both physiological regulation and pathogenesis of many diseases, including atherosclerosis, acute coronary syndrome, cardiac failure, aneurysm and tumors. This review focuses on the association of the anoikis with instability and rupture of atherosclerotic plaque.

Objective To investigate the perfusion characteristics of normal pancreas and pancreatic carcinoma on multi-slice spiral CT(MSCT).Methods Perfusion CT for pancreatic cancer was performed with sixteen-slice spiral CT in 26 patients(all of them were then confirmed by pathology).Eighteen patients without pancreatic disorders served as controls.Contrast medium-enhanced dynamic CT was performed,and the data were analyzed by using commercial software to calculate tissue blood flow(BF),blood volume(BV),mean transit time(MTT),and vascular permeability-surface(PS)area product.Perfusion parameters of normal pancreas and tumors tissues were compared.Results Technical failures were experienced in 1(3.85%)subjects of pancreatic carcinoma.The mean BF,BV,MTT and PS in pancreatic carcinoma were(68.89?73.42)mL/(100 mg? min),(10.02?3.59)mL/100 mg,(8.23?3.26)s,(28.63?9.35)mL/(100 mg?min),respectively.The mean BF,BV,MTT and PS in normal pancreas tissue were(173.53?71.33)mL/(100 mg?min),(14.43?3.15)mL/100 mg,(7.15?5.45)s,(13.98?8.53)mL/(100 mg?min),respectively.The mean BF,BV and PS between pancreatic carcinoma and normal pancreas were statistically significant(BF,PS:P0.05).Conclusion CT perfusion imaging can be used to identify patients with pancreatic carcinomas,because individual differences exist in the results of CT perfusion.

With the further deepening of the information revolution, traditional Chinese medicine, information science and technology are combined to give birth to Chinese medicine information science. Chinese medicine information science is developed and evaluated by mutual integration of Chinese medicine, computer science and information science, which is composed of the acquisition, processing and interpretation of method and system of patients’ data. Traditional Chinese medicine information technology can be used for ophthalmology of traditional Chinese medicine, so as to solve its dilemma, which there is no set of recognized system of syndrome differentiation and diseases are difficult to combine, with a purpose to take the advantage of traditional Chinese medicine in health management of ophthalmology diseases, and provide new possibilities for the development of traditional Chinese ophthalmology.

Hepatocellular carcinoma(HCC)shows high malignancy and metastasis. Hepatitis B virus X protein (HBx), a virus protein coded by hepatitis B virus, plays an important role in HCC metastasis. Recently, it was reported that HBx activates metastatic signal-pathways through MMPs, VEGF, uPA etc.,and promotes metastasis of hepatocellular carcinoma.

OBJECTIVE: Controversy over the presence of neuronal apoptosis in acute brain injuries has not been resolved due to the lack of direct evidence. In this article, the authors briefly review the current research progress of apoptosis and discuss the relationship between acute brain injuries and neuronal apoptosis.DATA SOURCES: A computer-based retrieval for relevant papers with abstracts indexed in Medline database published in English full text between January 1995 and February 2005 was conducted using the key words of "acute brain injuries" and "Neuronal apoptosis". Related articles in Chinese full text published between January 2000 and December 2004 were also searched in Chinese periodical full text database, Wanfang databases with key words of "acute traumatic brain injury, neuronal apoptosis".STUDY SELECTION: The retrieved articles were first examined to exclude review articles and repetitions, and only randomized and non-randomized controlled experiments or clinical trials on acute traumatic brain injury and neuronal apoptosis were included.DATA EXTRACTION: Altogether 136 articles directly related to acute traumatic brain injury and neuronal apoptosis were collected, among which 19 met the inclusion criteria with the other 117 removed for repetition or irrelevancy.DATA SYNTHESIS: The valid articles were reviewed to identify the factors related to neuronal apoptosis, apoptotic network theory, detection methods of apoptosis, and the relationship between acute traumatic brain injury and neuronal apoptosis as well as the possible mechanisms.CONCLUSION: Neuronal apoptosis occurs after acute traumatic brain injury, which induces intrinsic and extrinsic neuronal environment changes including increased extracellular excitatory amino acid content, massive free radical production and abnormal gene expression. These factors interact with each other and modulate neuronal apoptosis through multiple pathways.

Transient ischemic attack (TIA) is the most important early warning signal of cerebral infarction, and it is also the critical period for the prevention of cerebral infarction. If we effectively control the risk factors of TIA, the onset and the progress of TIA can be prevented and controlled, This article reviews the correlation between the risk factors of TIA and prognosis.

Juvenile myopia has become the most prominent issues to students' healthy growth in recent years,attracted widely concerns in the community.Evidence-based eye scientific research has shown that there is no method to cure myopia.Preventive treatment explores the unique effects of early interventions by providing patients with guidance on young people's daily life,diet and myopic prevention measures,which may effectively improve the incidence of juvenile myopia and has well social benefits.