The internet addiction disorder is an important problem under the internet circumstance.Improving self-regulated ability is a good method to avoid the problem.The article uses the selfregulated circle theory to gain the self-regulating strategy in three phases:forethought,performance or volitional control,self-reflection.These strategies include making goal,planning strategy,self-observation and self-record,attribution.

Objective To screen enterohemorrhagic Escherichia coli(EHEC) strain and declare it as a standard strain of China Medical Bacterial Species Conservation and Management Center(CMCC).On the base,to prepare strain reference and genomic DNA reference of EHECand declare them as national drug reference with independent intellectual property rights in our country.Methods According to GB4789.6-2016 National Food Safety Standards-Food Microbiology TestDiarrheagenic Escherichia coil Test,the EHEC strain was screened from 160 Escherichia coli strains from patients with diarrhea and declared as a standard strain of CMCC according to management regulations.EHEC bacterial solution and genomic DNA solution were prepared,and freeze-drying technology was used to prepare 600 strain(10~3 CFU/sample) and genomic DNA(20 ng/sample) samples respectively.20 strain and 20 genomic DNA samples were randomly selected for uniformity test.Samples storing at 25 ℃ and 37 ℃ for 1,3,5 and 7 d were taken respectively to test the transportation stability.Then the samples were tested for the short-term storage stability by storing at 4 ℃ for 7,14 and 28 d,and for the long-term storage stability by storing at 20 ℃ for 14,28 and 60 d.Three laboratories were organized for collaborative calibration.20 food products were chosen as the substrate to evaluate the application effect of strain samples.Results The one EHEC strain selected from 160 Escherichia coli strains from patients was finally declared as the CMCC(B) 43207standard strain.In the uniformity test,F_(strainsample)=0.662 0.05,and eae,stxl and stx2 of 20genomic samples were all positive.After storage at 25 and 37 ℃ for 7 d,-20 ℃ for 60 d and 4 ℃ for 28 d,the viable bacteria content of the strain samples was still 103 CFU/sample,and eae,stxl and stx2 of the genomic samples were positive.EHEC strains and genomic DNA samples selected randomly were identified as EHEC by three laboratories,the viable bacteria content was 10~3 CFU/sample,and eae, stxl and stx2 were all positive.It was detected in 20 kinds of food substrates after adding samples,but not in the background control.EHEC strain and the genomic DNA sample were included in drug standard materials in our country,numbered 80024 and 80056,respectively.Conclusion The prepared EHEC strain and genomic DNA standard materials with independent intellectual property rights can improve the timeliness of EHEC testing and make up for the gap in our country.

Anoikis is a special programmed cell death and plays important roles in both physiological regulation and pathogenesis of many diseases, including atherosclerosis, acute coronary syndrome, cardiac failure, aneurysm and tumors. This review focuses on the association of the anoikis with instability and rupture of atherosclerotic plaque.

Objective To investigate the perfusion characteristics of normal pancreas and pancreatic carcinoma on multi-slice spiral CT(MSCT).Methods Perfusion CT for pancreatic cancer was performed with sixteen-slice spiral CT in 26 patients(all of them were then confirmed by pathology).Eighteen patients without pancreatic disorders served as controls.Contrast medium-enhanced dynamic CT was performed,and the data were analyzed by using commercial software to calculate tissue blood flow(BF),blood volume(BV),mean transit time(MTT),and vascular permeability-surface(PS)area product.Perfusion parameters of normal pancreas and tumors tissues were compared.Results Technical failures were experienced in 1(3.85%)subjects of pancreatic carcinoma.The mean BF,BV,MTT and PS in pancreatic carcinoma were(68.89?73.42)mL/(100 mg? min),(10.02?3.59)mL/100 mg,(8.23?3.26)s,(28.63?9.35)mL/(100 mg?min),respectively.The mean BF,BV,MTT and PS in normal pancreas tissue were(173.53?71.33)mL/(100 mg?min),(14.43?3.15)mL/100 mg,(7.15?5.45)s,(13.98?8.53)mL/(100 mg?min),respectively.The mean BF,BV and PS between pancreatic carcinoma and normal pancreas were statistically significant(BF,PS:P0.05).Conclusion CT perfusion imaging can be used to identify patients with pancreatic carcinomas,because individual differences exist in the results of CT perfusion.

Objective To conduct the performance verification of the Stago STA-R automatic blood coagulation analysis system once every year to evaluate the analysis system for ensuring its accuracy and reliability .Methods According to the related docu-ments of the Clinical and Laboratory Standards Institute (CLSI) ,5 items of prothrombin time (PT ) ,activated partial clotting en-zyme live time (APTT) ,thrombin time (TT) ,fibrinogen (Fib) and antithrombin Ⅲ (ATⅢ) were selected to conduct the verifica-tion and preliminary evaluation on the accuracy ,imprecision ,carry-over rate ,linearity range and reference interval of the instrument analysis system .Results The relative bias(SE% ) of accuracy in each item was less than 5% .The intra-batch and inter-batch im-precisions (CV) were less than 3% and 5% respectively .The carry-over rates were within 4% .The linearity of Fib and AT Ⅲ was very well ,r2 were 0 .9980 and 0 .9979 respectively .Validated reference interval was consistent with quotative reference range . Conclusion The Stago STA-R automatic blood coagulation analysis system has good analytical performance ,can provide accurate and reliable test results for clinical doctors .

Purpose To evaluate the left ventricular regional diastolic function in coronary heart disease (CHD) patients without regional wall motion abnormality by real-time three-dimensional speckle tracking imaging (RT3D-STI) to investigate the value of RT3D-STI in the diagnosis of early CHD. Materials and Methods 140 CHD patients with 420 coronary artery main branches were enrolled and divided into 4 groups according to the results of coronary angiography (CAG): normal group (101 coronary artery branches without stenosis), mild stenosis group (112 coronary artery branches with stenosis<50%), moderate stenosis group (95 coronary artery branches with stenosis 50%-75%) and sever stenosis group (112 coronary artery branches with stenosis>75%). Three dimensional full volume images were collected through the chest, and the corresponding strain imaging diastolic index (SI-DI) of each direction was calculated, their value for the diagnosis of coronary artery disease was analyzed. Results The longitudinal, area and radial SI-DI of mild coronary artery stenosis group were lower than those of normal group (P<0.05);the longitudinal, area, radial and circumferential SI-DI of moderate coronary artery stenosis group were lower than normal group (P<0.05) and the area, longitudinal and circumferential SI-DI were lower than those of the mild stenosis group (P<0.05);the longitudinal, area, radial and circumferential SI-DI were all lower than those of normal and mild stenosis group (P<0.05), and the area, longitudinal and radial SI-DI were all lower than the moderate stenosis group (P<0.05). ROC curve showed that the sensitivity of longitudinal SI-DI (84.0%) was highest, while the specificity of area SI-DI (78.9%) was the highest. Conclusion RT3D-STI has important significance for early detection and diagnosis of CHD, regional diastolic function decrease of the coronary artery supply area occurs earlier than systolic function reduction, and diastolic function is more sensitive for myocardial ischemia, as a result, SI-DI is able to reflect the stenosis degree of coronary artery in a certain extent.

Preterm birth is the core issue of neonatology and perinatology researches,and the incidence of preterm birth increasing overall in recent years.With the development of modem health care,preterm birth survival rate has been increasing to some extent,while the prognosis is poor because of serious complications.Epidemiological studies of preterm birth is essential for improving the diagnosis and treatment of premature infants,and this summary introduces the clinical epidemiology of preterm birth through the definition,incidence,related factors,major complication and so on.

Hepatocellular carcinoma(HCC)shows high malignancy and metastasis. Hepatitis B virus X protein (HBx), a virus protein coded by hepatitis B virus, plays an important role in HCC metastasis. Recently, it was reported that HBx activates metastatic signal-pathways through MMPs, VEGF, uPA etc.,and promotes metastasis of hepatocellular carcinoma.

Juvenile myopia has become the most prominent issues to students' healthy growth in recent years,attracted widely concerns in the community.Evidence-based eye scientific research has shown that there is no method to cure myopia.Preventive treatment explores the unique effects of early interventions by providing patients with guidance on young people's daily life,diet and myopic prevention measures,which may effectively improve the incidence of juvenile myopia and has well social benefits.