Objective To clone and characterize the NADH1 gene of Cysticercus cellulosae. \ Methods\ A \{cDNA\} library was constructed from Cysticercus cellulosae and was immunoscreened by using rabbit anti\|Cysticercus cellulosae \{polyclonal\} antibody. The gene structure and its possible function were analyzed by comparing with sequences available in the GenBank, after the insert of positive clone was subcloned and the nucleotide sequence of the insert was \{determined\} by dideoxynucleotide chain termination method. \ Results and Conclusion \ A cDNA clone (named TS5) with a length of \{1 082\} bp was isolated. The 5′ terminal of cloned gene contained one open reading frame of 1-578 bp encoding 192 amino acid residues of mitochondrial NADH dehydrogenase subunit 1 and the 3′ terminal contained three kinds of tRNA genes.

Objective To understand the effect of environment variables on anxiety in the soldiers at high altitude.Methods A total of 267 soldiers at high altitude were investigated with the self-rating anxiety scale,the environmental scale and Eysenck personality questionnaire.Results Compared with norm(29.78±0.46),the soldiers at high altitude had higher sore of anxiety(37.22±6.64;t=17.92,P＜0.01).One-way ANOVA and independent.samples t test showed that the anxiety(38.37±5.93,36.65±6.951,36.75±6.88)of military people at high altitude had not evidently difference in different altitude(≥3587.5m,3587.5～2767.9m,≤2767.9m).Parental culture.length of military service(F=3.25,P＜0.05)and neuroticism(R=0.52,P＜0.01)had evidently effect on the depression of soldiers.Conclusion The occurrence of anxiety in the soldiers at high altitude has a higher level.Parental culture.1ength of military service and neuroticism had predictive value for depression.

Objective:To investigate the effect of initiator on pulsed Nd:YAG laser in the acid resistance of human teeth in vitro. Methods:A total of 25 enamel-samples and 25 dentine-samples were divided into laser plus initiator (a layer of Beijing black ink) group and laser only group.Each of the two groups was divided into five subgroups.The samples in the 5 subgroups were irritated by Na:YAG laser with an energy(J/cm2) of 0,20,30,40 and 50 respectively. Then all samples were immersed in 0.1 mol/L lactic acid demineralization solutions(pH 4.5) for 48 h at 37 ℃. The concentration of Ca 2+ (?10 -6 g) dissolved in the solution was measured by the calcium selective electrode. Results:Laser of 20-50 J/cm2 decreased the Ca 2+ concerntration in demineralization solution(P0.05).The initiator plus laser of 30-40 J/cm2 decreased the Ca 2+ release of dentine samples than laser only group (P

Making use of information technology to build a scientific education information system and to improve clinical teaching quality is an important way to transform traditional teaching model and management mode.In terms of characteristics of education management and clinical work,the article explores the construction of education information system which is based on B/S structure and has six function modules.Besides,the article details the content of each function module.

In this study, endogenous digoxin-like substance (EDLS) in the plasma was measured by radioimmunoassay in patients with congestive heart failure The EDLS levels were significantly increased in patients with heart failure and were positively correlated correlated with the heart function state, Na~+/K~+-ATPasc activity in red cells and the electrolyte change in blood cells. We believed that digoxin-like substance may participatc in the process of the compensatory mechanirsms of the heart failure.

Thyroid nodule has an increasing prevalence during pregnancy,which might caused by the change of internal environment.The research regarding the potential factors of thyroid nodule in pregnancy and approach for the pregnant with thyroid nodule is ongoing.So what's reviewed here is about the specialties of thyroid nodule in pregnancy and the strategies for them.

OBJECTIVE: To investigate the preventive and therapeutic effects of Danshen Decoction on gastric ulcer in experimental animal models. METHODS: The model of water immersion restraint stress ulcer in mice, and the models of acetic acid impaired gastric ulcer and pyloric ligation gastric ulcer in rats were established. The total gastric acid, the activity of pepsin and the amount of gastric wall binding mucus were detected. RESULTS: Danshen Decoction reduced the acute gastric mucosal lesion. The effect of 5.0 or 10.0 g/kg group was obviously better than that of the normal saline control group (P<0.05 and P<0.01 respectively). Danshen Decoction accelerated the healing of ulcer induced by acetic acid significantly, and increased the content of gastric wall binding mucus (P<0.01); and it also inhibited the formation of gastric ulcer induced by pyloric ligation, while having no influence on the secretion of gastric acid and the activity of pepsin. CONCLUSION: Danshen Decoction has a significant anti-ulcerative effect, which may be related to improving the gastric mucosal defensive function.

Objective To observe the effect of Danshen Yin on some gastric mucosal defensive factors in rats with acetic acid-induced gastric ulcer and to explore its anti-ulcer mechanisms.Methods Rat models with acetic acid-induced gastric ulcer were treated with Danshen Yin,ranitidine,and saline respectively for 14 days. After treatment,the ulcer index,serum nitric oxide(NO) level,plasma prostaglandin E2(PGE2)content and the number of micrangium in the granulation at the ulcer base were examined.Results The ulcer index in Dandhen Yin group and ranitidine group was significantly less than that in model group(P

Objective To investigate the effects of pulsed Nd∶YAG laser combined with NaF solution on acid resistance of human dental enamel and dentin in vitro Methods A total of 20 enamel samples and 20 dentin samples were treated with both laser plus NaF, laser only, NaF only, or no treatment(control) Then all samples were immersed in lactic acid demineralization solution(pH 4 5) for 48 h at 37 ℃ The concentration of Ca 2+ (?10 -6 g) dissolved in the solution was measured by atomic absorption spectrophotometry Results In both enamel and dentin samples, the lowest mean Ca 2+ concentration was recorded in laser plus NaF treated group (62 055?5 879,137 474?4 444)?10 -6 g The control samples showed the highest mean of Ca 2+ concentration (143 760?5 443 and 182 726?16 115)?10 -6 g The mean Ca 2+ concentration in laser plus NaF - or NaF - or laser treated group decreased significantly as compared with that in the control( P

AIM: To investigate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2, also known as FLK-1) in laser-induced choroidal neovascularization (CNV) in mouse.METHODS: CNV was induced in C57BL/6 mouse eyes by krypton laser photocoagulation. Choroidal fiuorescein angiography and histopathological examination were used to assess the development of experimental CNV. Cryostat sections from lesions on day 10 after laser treatment and normal eyes were prepared for Immunohistochemistry for FLK-1.RESULTS: Laser-induced CNV developed in all lesions on day 10. The expression of FLK-1 was detected in endothelial cells, retinal pigmented epithelium (RPE)-like cells and fibroblast-like cells in neovascular lesions. In normal adult mouse retinas, FLK-1 expression was mainly observed in RPE cells, inner nuclear and ganglion cell layers.CONCLUSION: Our findings demonstrated that expression of FLK-1 may play a role in the formation of laser-induced CNV in mice, which suggest that FLK-L may be a promising potential target for antiangiogenesis therapy for CNV.