Objective To develop an HPLC analytic method for determination of cyromazine residue in water.Methods Chromatographic column of YMC C18(150 mm?4.6 mm,5 ?m)was used,mobile phase was methanol+0.02 mol/L NH4Ac=15+85(V/V),flowing rate was 1.0 ml/min,DAD detection wavelength was 210 nm.Results Regression equation was y=243.8x-4.2,r=0.999 96.The minimal detectable concentration was 0.010 mg/L;the rate of recovery was 94%-100% and the relative standard deviation was less than 3.0%.Conclusion The method is satisfactory in precision,accuracy and sensitivity.

OBJECTIVE:To determine the concentration of cefdinir in rat plasma,and investigate its pharmacokinetics. METH-ODS:High performance capillary electrophoresis (HPCE) was adopted by using fused-silica capillary (75 μm,total length of 30 cm,effective length of 21.5 cm),buffer solution of 20 mmol/L citric acid,injection voltage of 10 kV for 10 s,separation voltage of -20 kV,and detection wavelength of 214 nm. 6 rats were intragastrically received cefdinir solution(20 mg/kg). 0.2 mL blood sample was taken from the tail vein before and 0.25,0.5,1,1.5,2,3,4,6,12,24 h after administration. BAPP 2.0 software was used to calculate the pharmacokinetics parameters. RESULTS:The linear range of cedinir ranged 0.2-50 μg/mL(r=0.9997), lower limit of quantification was 0.2 μg/mL. The intra-day(n=6)and inter-day RSDs of(n=3)precision test were no more than 12.2%;RSD of stability test was no more than 9.10%(n=6);method recovery rate was 95.4%-114.3%(RSD=9.0%,n=6);matrix effect was 63.5%-70.2%(RSD=10.39%,n=6). The t1/2 of cefdinir in rats in vivo was(0.54±0.01)h,MRT was(1.90± 0.14)h,cmax was(32.92±0.81)μg/mL,tmax was(1.50±0.02)h,AUC0-24 h was(46.65±0.44)μg·h/mL and AUC0-∞was(46.83± 0.44)μg·h/mL. CONCLUSIONS:The method is rapid,accurate and simple,and can be used for the determination of cefdinir con-centration in rat plasma and its pharmacokinetics research.

Objective To observe the effect of Jianpi Buxue Decoction on the protein expression of neurokinin 1 receptor (NK1R) and CD34 in C57BL/6 mice of lung cancer after chemotherapy, and to explore the improvement of chemotherapy-induced nausea and vomiting and myelosuppression treated with Jianpi Buxue Decoction. Methods Forty C57BL/6 mice were transplanted with Lewis lung cancer cells in the armpit of left anterior limb after being fed for 7 days, and then were randomly divided into lung cancer group, model group, and high-, middle- and low-dose Chinese medicine groups. Model group and Chinese medicine groups were injected intraperitoneally with cyclophosphamide (80 mg/kg) for one day. Lung cancer group and model group were given normal saline. Chinese medicine groups were administered with high-, middle- and low-dose of Jianpi Buxue Decoction (25, 12.6, 6.25 g/kg respectively) for 14 days. After the modeling, all of the mice were sacrificed, and then the brains and spleens were sampled. Western blotting method was used to detect the protein expression of NK1R and CD34. Results Compared with lung cancer group, the protein expression of cerebral NK1R and splenic CD34 in the model group was increased significantly ( P<0.01) . Compared with the model group, the protein expression of NK1R in mice brain tissues of high-, middle- and low- dose Jianpi Buxue Decoction groups was decreased significantly (P<0.01) , while the protein expression of CD34 in spleen tissues of middle-and low-dose Jiangpi Buxue Decoction groups was increased obviously ( P<0.05) . Conclusion Jianpi Buxue Decoction has an effect on down-regulating the protein expression of NK1R in brain tissues and on up-regulating the protein expression of CD34 in spleen tissues of C57BL/6 mice with lung cancer after chemotherapy, indicating that Jianpi Buxue Decoction probably can relieve chemotherapy-induced nausea and vomiting, and can improve the myelosuppression after chemotherapy.

Objective To analyze the different spreading patterns and the prognostic factors of nasopharyngeal adenocarcinoma (NPAC).Methods The clinical and radiological data of 39 pathologically confirmed consecutive cases of NPAC were retrospectively reviewed.Results In the 1 1 patients with CT/MRI-detected cranial nerve (CN)involvement,there were ACC (n=7),CAC (n=2)and MEC (n=2)(P <0.05).Lymph node metastasis occurred in 40% of CAC,7.7% of ACC and 1 6.7% of MEC (P =0.035). Significant differences were observed in 5 year OS and DFS rates between patients with and without CT/MRI-detected CN involve-ment (P <0.001;P =0.001).In 1 9 patients with early stage disease (stageⅠ-Ⅱ),significant difference was observed in 5 year OS rate between the surgical treatment group and non-surgical treatment group (P =0.038),and the difference was not significant in DFS (P =0.056).Conclusion CT and MRI are valuable tools not only for discovering the different spreading patterns of NPAC, but also for its prognosis.

Objective To investigate the clinical efficacy of Ilizarov bone transport combined with dressing change in the treatment of acute tibial osteomyditis accompanied with massive soft tissue defection.Methods From July 2009 to september 2012, 14 patients with acute tibial osteomyelitis associsted with large-area soft tissue necrosis and defection were treated in Traumatic Orthopaedics Department of the Central Hospital of Binzhou.All cases were Gustillu Ⅲ type open fracture, and Ⅲ C type injury in 11 casas accompanied with vascular injure.Associated with contralateral lower limb in 4 casas.They were 10 male and 4 female,aged from 21 to 45 years old(average age of 35 years old).The duration from injury to surgery ranged from 12-24 days,averaging of 19 days.All cases were treated first with excision of the infected or necrosis bone segment and soft tissue.The wound keep opening, followed by llizarov bone transport combined with dressing change to repair the bone and soft tissue defection.The clinical efficacy was assessed using the Paley's evaluation system at the last follow-up.Results All the patients were followed up for 24 to 36 months (average of 27 months).The bone defection rapaired was from 5 to 1 3 cm(average of 7 cm).The mean duration of distraction was 4 months, range from 2 to 6 months.All cases' gain s.ufficient soft tissue coverage the later period of Ilizarov bone transport.All the fracture and bone defection were cured.Infection were cured in 13 cases, and 1 case converted to chronicle osteomyelitis, and the cure rate was 93％.According to the Paley' s system, the bone union was excellent in 11 cases, good in 2 cases, and fair in 1 Cases, with a good-to-excellent rate was 93％.The functional outcome was excellent in 10 cases,good in 2 cases,and fair in 2 cases,with a good-to-excellent rate of 86％.Conclusion Ilizarov bone transport and dressing change is one reliable and effective treatment of tibial acute infected and soft tissue defection.

ObjectiveA comparison between intragate and ECG-respiration triggered techniques was performed to determine their differences in measuring the structure and function of the heart at 7.0 T.MethodsTen normal ICR mice aged five to six weeks were examined on a 7.0 T MR scanner.A central slice with papillary muscle included at the short-axis view was scanned with a FLASH-cine bright blood sequence,FLASH-cine black blood sequence,IG-FLASH-sat-cine black blood sequence,and IG-FLASH-cine bright blood sequence.The area of the left ventricle of the end systole and end diastole ( including and excluding the myocardium) was measured with manually outlined ROIs.The increased area of the left ventricle and the myocardium from the end systolic to end diastolic phases was calculated.The signal intensity was measured from 8 ROIs which were evenly located at the myocardium of the end systole,and the mean and standard deviation were then determined.The coefficient of variation ( CV ) was derived by dividing the mean into the standard deviation.ResultsThere was no significant difference ( the increased area of the myocardium t =0,P =1,the increased area of the left ventricle t =2.12,P =0.06) in the function index between the ECG-triggered black blood sequences [the increased area of the myocardium (0.100 ±0.018) cm2,the increased area of the left ventricle (0.060 ± 0.024) cm2] and intragate black blood sequences[the increased area of the myocardium (0.090 ± 0.014) cm2,the increased area of the left ventricle (0.060 ±0.012) cm2].No significant difference(the increased area of the myocardium t =1.56,P =0.15,the increased area of the left ventricle t =2.08,P =0.07 ) in the function index was observed between the ECG-triggered bright blood sequences [the increased area of the myocardium ( 0.100 ±0.018) cm2,the increased area of the left ventricle(0.060 ±0.014) cm2]and intragate bright blood sequences [the increased area of the myocardium (0.090 ±0.019) cm2,the increased area of the left ventricle (0.050 ±0.015) cm2].Furthermore,there was no significant difference(t =1,P =0.34) in the CV of the myocardium signal intensity of bright blood sequences between the ECG-triggering ( 0.050 ± 0.013 ) and intragate (0.040 ± 0.015 ),but significant difference ( t =4.51,P =0.001 ) in the CV of the myocardium signal intensity of black blood sequences between the ECG-triggering ( 0.070 ± 0.033 ) and intragate ( 0.160 ± 0.046 ) was obtained.ConclusionsThe intragate sequences could take the place of the ECG gate sequences in functional analysis of the heart( including bright blood and black blood sequences).The bright blood intragate sequences also could replace the bright blood ECG-triggered sequences in analyzing the signal of the myocardium.

The reasons why the relationships between doctors and patients hasn't advanced to a full and true harmonious stage in our country are multiple.From the legal point of view,the research on the important legal issues of doctor-patient relationship is deficient.The law regulating the relationship is hysteretic and even lacking.The system settling the doctor-patient conflict is distempered and imperfect.Both the doctors' and patients' legal knowledge and consciousness are inadequate.Therefore,it is necessary to discuss the legal measures from the four aspects to construct a harmonious medical service system.

Objective To discuss the value of Valsalva experiment combined with improved MRI tilt tomography applying on the pharyngeal ostium of eustachian tube opening function of patients with nasopharyngeal carcinoma.Methods 1 7 cases with patholog-ically proved NPC underwent conventional MRI plain and enhanced scan.In the eustachian tube area oblique transection T2 WI and T1 WI scanning was done before and after the Valsalva experiment.The invasion of eustachian tube and its surrounding structures was analyzed and the eustachian tube function was evaluated.Results Valsalva experiments combined with improved MRI trans-verse oblique scan could clearly display the whole structure of the eustachian tube and surrounding tissue invasion compared with conventional scanning MR scan.And it could better evaluate the eustachian tube opening function for patients suffered nasopharynge-al carcinoma.Conclusion Valsalva experiments with improved MRI oblique transverse imaging could achieve individualized scan of the eustachian tube,and could be used for evaluating the pharyngeal opening function of eustachian tube in patients with nasopharyn-geal carcinoma.

In recent years,the course of pathogenic biology of Nanhua University has made great achievements because the discipline characteristics has been stressed.Therefore,the reputation of Nanhua University is improved with the elevation of this discipline.The development patterns of Nanhua University provide some experience for the building and development of general university.