Objective To investigate the protein and mRNA expression of NF-κB and zinc finger protein A20 in the keloid and normal skin and to explore its underlying molecular biological mechanism.Methods The protein and mRNA expression of NF-κB p65 and zinc finger protein A20 in keloid and normal skin was detected by immunohistochemistry and real time quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results The protein and mRNA expression of NF-κB p65 in keloid tissues was higher than those in normal skin tissues (P＜0.05).The protein and mRNA expression of zinc finger protein A20 in keloid tissues was lower than those in normal skin tissues (P＜0.05).Conclusions NF-κB signal pathways and zinc finger protein A20 could play a role in keloid pathogenesis.

Objective To investigate therapeutic effect of bromhidrosis by botulinum toxin A injections and situation of bromhidrosis recurrence.Methods Clinical data of bromhidrosis patients were analyzed in our hospital from January 2013 to June 2014.A total of 89 cases were involved in this study and randomized into two groups:observation group (42 cases) were treated by botulinum toxin A,and patients with the control group (47 cases) were treated by small skin excision.The total effective rate,incidence of complications and recurrence rate in both groups were compared.Results After treatment for 3 months,the total effective rate of both group had no statistical difference (P＞ 0.05).After different treatment,the incidence of complications in observation group of patients was 2.38％ (1/42),and that in the control group was 14.89％ (7/47);complication rates of control group patients was significantly higher than that of the observation group,with statistical significance (x2 =4.245,P＜0.05).After 9 months follow-up,31 cases in the observation group of patients showed bromidrosis recurrence,with recurrence rate of 73.81％;2 patients in the control group showed bromidrosis recurrence,with recurrence rate of 4.26％.Log-Rank test showed that recurrence rate in observation group was significantly higher than that of control group (x2 =43.21,P ＜0.05).Conclusions Botulinum toxin A injection is an uninvasive method for the treatment of bromhidrosis with fewer complications and good effect,but the postoperative recurrence rate is higher and it needs injections for many times.

OBJECTIVETo study the possibility of removal melanin granules from autogenic acellular dermal matrix of giant nevus tissue by H2O2 bleaching technique.

METHODSA total of 32 skin specimens (0.5 cm x 0.5 cm) from giant nevus tissue and 1 piece (0.5 cm x 0.5 cm) of normal skin were obtained from the surgical removal. One giant nevus tissue was chosen as control. The others and the normal skin tissue were treated with solution of 0.25% Dispase II for digestion for 24 hours under normal temperature to remove epidermis. Then each piece was immerged into solution of 0.5% Triton X-100 for digestion for 48 hours in normal temperature. One giant nevus tissue and the normal skin tissue were chosen as control. The others were immerged into solution of different concentrations of H2O2, treated under different temperature and lasting for different period. Lastly, all specimens were treated with HE staining, immunohistochemical staining, light microscopy and so on.

RESULTSAfter giant nevus tissues were treated with solution of 0.25% Dispase II and immerged into solution of 0.5% Triton X-100 in normal temperature, nevus cells and all other cellular components of pigmented nevus tissues can be effectively removed, there were the cavities left by removal of cells without any residual cell debris, but still remaining part of pigment. Then each specimen were immerged into solution of different concentrations of H2O2, under different temperature and lasting for different period which can remove residual melanin granules. In solution of 3% H2O2 for 36 h under 37 degrees C, can remove all the melanin particles, the content of collagen type I in the obtained specimen was not changed. Collagen fibers were uniform in thickness, regular in arrangement with no obvious degeneration.

CONCLUSIONSWith solution of 0.25% Dispase II and solution of 0.5% Triton X-100 in normal temperature, all cells in nevus tissue can be removed effectively. Further treatment with 3% H2O2 at 37 degrees C for 36 h can remove all the melanin particles, while collagen type I has no obvious change. The preparation of acellular dermal matrix of the giant nevus may possibly be applied as autologous tissue implant to repair tissue defects.

Acellular Dermis ; Endopeptidases ; pharmacology ; Epidermis ; Humans ; Hydrogen Peroxide ; pharmacology ; Melanins ; Nevus ; pathology ; Nevus, Pigmented ; pathology ; Octoxynol ; pharmacology ; Skin Lightening Preparations ; pharmacology ; Skin Neoplasms ; pathology ; Skin Pigmentation ; drug effects ; Skin Transplantation ; Surface-Active Agents ; pharmacology

Objective To investigate the aesthetic standard of leg length in young unmarried females.and to provide an additional reference for leg-plasty.Methods According to the data of the 531 young healthy women aged 17 to 20 years,the mean,standard of height,weight,hip circumference,lowaer limb length,leg length,leg girth,lower leg length-girth index,and leg girth index were calculated.Correlations between these parameters were analyzed by SPSS 15.0 software.Results Heightwas(163.22±4.03)cm,weight was(53.82±6.12)kg,hip circumference was (91.51±4.56)cm,whole leg was(75.95±2.97)cm,leg was(37.97±1.83)cm,length of leg maximal girth to tibial tuberosity(14.13±0.74)cm,length of leg medium girth to tibial tuberosity (18.99±0.92)cm,leg maximal girth(34.91±1.82)cm,leg minimal girth(20.30±1.06)cm,leg length to height ratio was 23.26±0.90,lower leg to whole leg ratio 50.01±1.95,leg maximal girth to hip circumference was 38.18±1.66,lower leg length-girth index was 92.12±5.86,leg girth index was 58.21±2.84.and the distance from leg maximal girth point to tibial tuberosity to leg length ratio was 37.26±1.78.Distance from leg maximal girth point to tibial tuberosity to leg length ratio and leg girth index had significant correlation with height,and hip circumference,but had no significant correlation with weight.Lower leg length-girth index had significant correlation with height,weight,and hip circumference.Conclusion These data have some reference value on analyzing the characteristics of human female leg cosmetology.

Objective To study the aesthetic standard and parameters of abdominal profile in young females, in order to provide anatomic data for abdomino plasty and umbilicus reconstruction or transposition. Methods Measurements were conducted in 281 young health females, including height, weight, abdominal length, abdominal width and umbilicus location. All data were analyzed. Results The abdominal length, abdominal width, length from appendix ensiformis to omphalion, length from appendix ensiformis to middle point between appendix ensiformis and symphysion were (37. 93 ± 2. 15), (36. 98 ± 2. 71), (21. 10±1.80), and (18. 96±1. 08) cm, respectively. Abdominal length was positively correlated with height. Abdominal width, and the ratio between abdominal length and width were positively correlated with weight. The location of umbilicus was lower 2. 1 cm than middle point between appendix ensiformis and symphysion. Conclusion The abdominal profile is significant for clinical reference during abdomino plasty and umbilicus reconstruction or transposition.

OBJECTIVETo investigate the application and therapeutic effect of frontal muscle fascia compound flap suspension for congenital blepharoptosis in children.

METHODSFrom July 2010 to March 2012, 21 children (23 eyes) with congenital blepharoptosis were treated with frontal muscle fascia compound flap suspension. The therapeutic effect was observed and followed up.

RESULTS17 patients were followed up for 3-6 months with relapse in one case. The ptosis was corrected in the other 1 cases.

CONCLUSIONThe frontal muscle fascia compound flap suspension is effective and practical with minimal morbidity and lower complication for the correction of congenital blepharoptosis.

Blepharoplasty ; methods ; Blepharoptosis ; congenital ; surgery ; Child ; Facial Muscles ; Fascia ; transplantation ; Frontal Bone ; Humans ; Oculomotor Muscles ; Recurrence ; Surgical Flaps ; transplantation

Objective To study what affect the incidence and recurrence of keloids. Methods A total of 476 cases were treated with surgery plus adjunctive radiation therapy, intraleisional injection of triamcinolone acetonaide acetate, or surgery plus postoperative topical medicine. The family history and other clinical data were analyzed, including patients' age, sex, the duration of keloids, initiating factors, location, size, treatment method, and recurrence of the keloids. Results Keloids occurred in both male and female with the rate of 1. 83 ∶ 1. 00, with a predominance in woman aged from 22 to 45 years old. Trauma and pierce were the most significant initiating factors. The recurrence was affected by patients family history, treatment methods and the location of keloids, and less affected by keloids size, patients' age and sex. Conclusions Both surgery following adjunctive radiation therapy and intraleisional Kenalog injection are useful methods, which are prior to surgery plus postoperative topical medicine. The keloids incidence has a preponderance in some patients with family history. Therefore,genetic research is important to identify the pathogenetic factors in the keloids.

Objective To investigate the methods and effects of anterolateral thigh flap reconstruction of the complicated hip decubitus.Methods The study contained 24 cases of grade Ⅳ hip decubitus reconstructed with anterograde pedicled anterolateral thigh flaps through May 2010 to July 2014.There were 13 males and 11 females,aged 26-64 years (mean 54 years).Defects ranged in size from 12 cm×6 cm to 24 cm× 12 cm.Dimensions of flaps harvested were 14 cm ×8 cm to 30 cm× 14 cm.Eighteen cases of the donor sites were directly sutured and six covered with full thickness skin.Results Twenty-two flaps survived completely,and wound was healed by the first intension.Two flaps developed partial distal necrosis,and wound was healed after dressing treatment.Period of follow-up was 6 months to 2 years.Shape,texture and color of the flaps were well matched with the recipient area.Conclusion The anterolateral thigh flap transplantation has advantages of similarity to the recipient area,large enough flap area and sound expected effective and is a good method for repair of hip decubitus.

Objective To investigate facial-cervical index difference in undergraduates of different height or weight in Han nationality to provide an additional reference for plastic surgery of facialcervical region.Methods Subjects consisted of 200 college undergraduates of Han nationality,aged 20 to 24 years,with average 22.9 ± 1.7,of whom 100 were male and 100 were female.Their height,weight,bizygomatic breadth,facial height,cervical height,neck circumference,neck breadth,shoulder width,cervical-mandibular angle,thorax-cervical angle,shoulder-cervical angle,pulvinar-cervical angle and back-cervical angle were measured and calculated.Results The differences among the indices mentioned above were of statistical significance (P＜0.05);the indices of female subjects of different height were significantly different (P＜0.05);the differences in indices of male subjects of different weight were of statistical significance (P＜0.05);the indices of female subjects of different weight were also statistically different (P＜0.05).The facial-cervical indices in both male and female subjects of different height or weight were of no stistical difference (P＞0.05).Conclusions Male subjects of different height or weight have different cervical height,cervical-mandibular angle,neck height and facial height indices are different;hence,the indices are related to body height;female subjects of different body height have different neck height.Subjects of different weight have different neck circumference,neck breadth,should breadth,shoulder-cervical angle and pulvinar-cervical angle.

Objective To explore the new way of administration and clinical effect of botulinum toxin A in the treatment of focal hyperhidrosis.Methods The clinical efficacy was observed in 132 sites of 28 patients with focal hyperhidrosis,and the degree and range of focal hyperhidrosis were determined by the minor iodine-starch test.50 U of botulinum toxin A was injected in unilateral axillary,palms and soles with BellaVita instrument and 30 U for forehead.Each patient was followed-up in 1 week,2 weeks and every month after injection for 8 months.According to the results of the minor iodine-starch test the objective effect and evaluation score were obtained,and the comprehensive effect evaluation score was calculated with the objective effect evaluation score and the subjective effect evaluation score in each follow-up.Results The comprehensive effect evaluation score before injection of botulinum toxin A was 1.34±3.94,and that after injection was 23.21±9.44 for 1 week,92.41±11.95 for 1 month,98.21±5.60 for 2 months,95.98±5.94 for 3 months,and 86.61±10.17 for 4 months,respectively.Compared with that before injection,the difference was statistically significant (P ＜0.05).The effect decreased slowly after 4 months of injection,and the efficacy was maintained for 8 months (4.46±6.98);compared with that before injection,the difference of the clinical efficacy was not statistically significant (P ＞0.05).Based on the comprehensive effect evaluation scores,the differ ence of the clinical efficacy was not statistically significant between 1 week and 6 months after injection (P＞0.05).Conclusions The clinical effect of botulinum toxin A injected by BellaVita is prompt and effective for focal hyperhidrosis.