Objective To study the distribution and drug‐resistance of pathogens isolated from vaginal secretion in the patients with vaginitis in Dalian area so as to provide an evidence for clinical diagnosis and treatment .Methods The statistical analysis was performed on the detection results of pathogens isolated from vaginal secretion in 5 308 patients with vaginitis .Results In 5 308 patients with vaginitis ,the routine detection of vaginal secretion found that 810 cases(15 .3% ) were bacterial vaginosis ,1 110 cases (20 .9% ) were candida positive and 60 cases (1 .1% ) were trichomoniasis positive ,in which 3 526 cases were performed the patho‐gen detection ,2 615 strains of pathogen were isolated ,including 1 948 strains (74 .5% ) of mycoplasma ,136 strains (5 .2% ) of Chlamydia ,17 strains (0 .7% ) of Neisseria gonorrhoeae ,276 strains(10 .6% ) of candida ,140 strains (5 .4% ) of Gram‐positive cocci and 98 strains(3 .7% ) of Gram‐negative bacilli .The susceptibility rates of Ureaplasma urealyticum to ofloxacin ,levofloxacin ,spar‐floxacin and roxithromycin were lower ,which were 7 .8% ,18 .2% ,26 .3% and 27 .2% respectively ,the susceptibility rates to clar‐ithromycin ,doxycycline and minocycline were higher ,which were 89 .4% ,99 .8% and 99 .9% respectively .The drug resistance of Mycoplasma humenis was higher .The more sensitive antibacterial drugs were only josamycin ,doxycycline and minocycline ,the sen‐sitivity rates were 75 .3% ,96 .2% and 97 .3% respectively ,which to other antibacterial drugs were less than 7 .0% .Candida albi‐cans was generally sensitive to antifungal drugs .The susceptibility rates of Staphylococcus aureus to commonly used antibacterial drugs were higher .All of 24 strains of Streptococcus agalactiae were sensitive to penicillin .No Gram‐positive coccus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to imipenem and meropenem was found .Conclusion The pathogens causing vaginitis are very complex .In the treatment of female vaginitis ,clinicians should rationally use antibacterial drugs according to the results of pathogens detection and drug susceptibility test .

microRNA (miRNA) is a class of endogenous small non-coding RNA (20-25 nt) which can regulate the expression of protein-coding genes post-transcriptionally and participate in a series of critical biological processes including cell proliferation,differentiation,metabolism,apoptosis,et al.Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma subtype.DLBCL is defined as the malignant lymphoma which originates from the B cells,with aggressive clinical behaviors and significant clinical heterogeneity.In recent years,the researches on miRNA in DLBCL have shown that several kinds of miRNA directly involved in the occurrence and development of tumors and there is a correlation between the miRNA expression profiles and molecular subtypes of DLBCL.As a kind of new molecular target,miRNA has a broad prospect in the application both for diagnosis and biotherapy of DLBCL.

Objective:To investigate the clinical features,pathological changes of 34 cases that had suffered infantile hepatic glycogen storage disease in order to improve the recognition.Methods:The clinical manifestations of 34 cases of infantile hepatic glycogen storage disease during May 2005 to May 2009 were collected.The hepatic tissues from liver biopsy were observed under microscope and electron microscope.Results:Hepatomegaly and growth retardation were the main clinical manifestations of hepatic glycogen storage disease, partial cases were complicated with the manifestations of hypoglycemia.Hypoglycemia,hyperlactacidemia and abnormality of liver function were detected.And the pathology features were observed under microscope and electron microscope.Conclusion:The clinical manifestations of infantile hepatic glycogen storage disease were common,and the final diagnosis can depende on liver biopsy.

Objective To detect the expression of mutational p53 and inducible nitric oxide synthase in ovarian neoplasm and study the correlation between them. Methods The expression of p53 gene and inducible nitric oxide synthase was detected by immunohistochemistry method. Results ①The expression of p53、iNOS in healthy ovary,benigh and malignant ovarian tumor increased gradually(909%、13.13%、65.31%;18.18%、20.00%、61.22%) (P

Objective To detect the expression of mutational p53 and inducible nitric oxide synthase(iNOS) in ovarian neoplasm,and study the correlation between them. Methods The expression of p53 gene and inducible nitric oxide synthase was detected by immunohistochemistry method.Results(1)The expression of p53、iNOS in healthy ovary,benigh and malignant ovarian tumor increased gradually(9.09%,13.13%,65.31%;18.18%,20.00%,61.22%) P

Objective] The article summarizes the experience of using sanren decoction to treat pediatric diseases by Professor Liu Lin, the famous TCM physician of Hubei. [Methods] Through following Professor Liu Lin clinical diagnosis, organize his medical records, summarize the experience and thoughts of using Sanren decoction to treat pediatric diseases, and for some cases. [Results] Professor Liu Lin believes that the physiological and pathological characteristics of children prone to cause hot and humid accumulation, pediatric Chih-Yin is not substantial, with their lungs often delicate and spleens often inadequate, they prone to get hot and humid accumulation after sick, and later appear a variety of symptoms including upper burner, center burner and lower burner. Therefore, the treatment of pediatric disease should take the heat into account, bearing in mind the residence site of heat evil and syndrome differentiation. Professor Liu Lin makes good use of Sanren decoction to treat a variety of pediatric diseases, judging the residence site of heat evil and clinical manifestations, giving the corresponding treatment flexibly. Such as the treatment of bronchitis with almonds as the monarch herb to expectorant cough, treatment of infantile anorexia with White Kou Ren as the monarch herb to activate spleen and remove dampness, treatment of children with nephrotic syndrome with Yiyiren as the monarch herb for penetration of water, treatment of HFMD with the Sanren to regulate Qi of triple burner, according to the symptoms to medicate and always has good clinical effect. [Conclusion] Professor Liu Lin using Sanren decoction to treat pediatric diseases is effective, his thought is worthy of depth study and promotion.

Objective:To study the inhibitory effects of adenovirus mediated vascular endothelial cell growth inhibitor 151(VEGI_(151)) on venous endothelial cell proliferation. Methods: VEGI_(151) gene was cloned into adenovirus vector pCA13 and the product was packaged and amplified with 293A cells.The expressed protein was identified by Western blot. X-gal staining was used to determine the transfection efficiency of the recombinant adenovirus system. The inhibitory effect of Ad-VEGI_(151) on ECV304 cell proliferarion was observed and assessed by violet crystal assay. The protein expression of VEGI_(151) gene in ECV304 cells was detected by immunohistochemistry. Results: Liposome-mediated pCA13-VEGI_(151) and pJM17 cotransfection of 293A cells (through homologous recombination of intracellular plasmid) is a practical and feasible method to obtain high-titre recombinant adenovirus. The constructed Ad-VEGI_(151), with high transfection efficiency, significantly inhibited ECV304 cells proliferation and expressed functional protein in target cells. Conclusion: Ad-VEGI_(151) can express bioactive protein in target cells and can inhibit the proliferation of venous endothelial cells, and this may pave a new way for gene therapy of tumors and neovascular diseases.

Antithyroid drugs(ATD)is the main treatment for hyperthyroidism and its adverse reactions have been much concerned by physicians. Methimazole(MMI)and propylthiouracil(PTU)are the two common antitithyroid drugs used currently. Generally, the ATD are safe and effective, though their clinical adverse reactions are also relatively common. The toxic effects include liver damage and leukocytopenia, antineutrophil cytoplasmic antibody-associated pulmonary small-vessel vasculitis, hypoglycemia, allergic reactions, muscle impairment,and so on. They are usually reversible and disappear spontaneously when the drug is discontinued. However,the serious rare side effects can also occur and there may have potentially deadly threatening effects which need to be cautious for the clinicians. MMI is usually preferred over PTU because it has significantly fewer side effects. And unlike the dose-dependent side effects of MMI, there has no significant correlation between adverse reaction and drug dosage in using PTU. Moreover, PTU has more severe hepatotoxity than MMI, even fatal liver impairment and liver failure. The risk of liver damage from PTU is an important concern, particularly in children. For this reason, MMI is the first choice for treating children with hyperthyroidism.

Objective To discuss the pathogenesis and clinical characteristics of acute relapsing pancreatitis .Methods The clini-cal data and relevant data of 274 acute pancreatitis cases from January 2010 to and December 2012 were analyzed ,of which one group of 231 cases were first onset and the other group of 43 cases were relapsing .Results The probability of relapse of acute pan-creatitis were highest from 1 month to half a year after first onset (20 cases ,accounting for 46 .51% );biliary factors were the major causes of first onset and relapse cases ,where the relapsing group was higher than first onset group ,with statistical significance (P<0 .05);the relapsing group had more severe symptoms of hyperlipidemia than the first onset group ,with significant difference (P<0 .05) ,The relative risk (OR value) of relevant factors were higher than biliary factors ;improper diet ,alcohol and other factors of the relapsing group were lower than the first onset group ,without statistical significance (P> 0 .05);there was no significant difference between the occurrence rate of severe pancreatitis and the occurrence rate of surgeries and deaths .Conclusion Biliary diseases and hyperlipidemia are closely related with the relapse of acute pancreatitis .

In this article, we summarize the main policy on encouraging nongovernmental investors to establish medical institutions in Wenzhou , Zhejiang Province.Based on the survey of private medical institutions in Wenzhou , we find that there are still many issues that should be resolved in terms of nongovernmental investors establishing pri -vate hospitals , such as the difficulty in implementing construction projects , spending too long time applying for the medical insurance unit , the complicated application procedures of incentive funds , the unequal status with public health hospitals in financing and the development of human resource for health , and the low-level administration and social reputation.Therefore , for promoting the sustainable development of private health hospitals , it is of great im-portance to have a breakthrough in the implementation of the policy ,such as supporting policies reform , financing and talent support , strengthening supervision , and so on.