A method of preparation of Chondroitin su(?)fate from Raja porosa cartilage was presented, using the extraction process of increasing concentration of strong bases, the hydrolysis of complex enzyme and the alcohol precipitat(?)on method. The technologic condition was researched. The white powder of Chondroitin sulfate was obtained at the yield rate of 2.82% and the purity of 93.6%.

Objective:To observe the clinical efficacy of electroacupuncture and rehabilitation exercises in the treatment of cerebral infarction.Methods:Seventy-four patients with cerebral infarction were randomized into treatment group and control group in visit sequence.Thirty-seven cases in treatment group were treated with electroacupuncture and rehabilitation exercises,and other 37 cases in control group were treated with electroacupuncture alone.The treatment was given once a day and there was a 2-day interval after any consecutive 5 treatments.After total 30 treatments,Fugl-Meyer scale was used to evaluate motor function and Barthel Index to evaluate the activities of daily living.Results:After treatments,the motor function of paralyzed limb and activities of daily living were obviously improved in both groups,with more significant improvement in treatment group than in control group (P＜0.05).Conclusion:Combination of electroacupuncture and rehabilitation exercises has better effects than simple electroacupuncture in the treatment of cerebral infarction.

Objective To investigate the clinic effect of hypocellular acute myelogenous leukemia (AML) patients with CAG regimen. Methods 29 cases with hypocellular AML were treated with CAG regimen. Results There were 14 cases achieved complete remission (CR), accounting for 48.3 %; 7 cases partial remission (PR), accounting for 24.1%. The total effective rate was 72.4 %, inducing 7 cases of NR, one case dead in early time. Conclusion CAG regimen has been proved to be effective in shortening the period of reduced peripheral blood granulocytes and decreasing the death rate related to chemotherapy, hence,a secure and effective therapy in treating hypocellular AML.

Objective To investigate the effect of continuous blood purification(CBP) in the patients with severe acute pancreatitis(SAP). Methods Twenty-six patients with severe acute pancreatitis were divided into the control group and the observation group randomly. Fourteen cases in the control group were given conventional treatment only, 12 cases in CBP group were given continuous blood purification as well as the conventional treatment. The APACHE Ⅱ score and CRP of pre- and intra-treatment at the 2nd and 5th day were observed in the two groups. The duration of mechanical ventilation, the duration of ICU stay, the mortality of hospital were observed as well. Results The APACHE Ⅱ score and CRP of the second day and the fifth day of intra-treatment in the control group were 1.61±3.00,10.76±4.30, (149.71±60.06) mg/L and (101.21±33.98) mg/L, respectively, which were significantly different to those of (8.20±1.87) mg/L, (6.19±1.49) mg/L, (109.17±23.91) mg/L,(70.83±25.45)mg/L in the CBP group(P＜0.05). The duration of mechanical ventilation,the duration of ICU stay and the mortality of hospital were (13.29 ± 6.23) d, (16.00 ± 6.86) d, 21.43% in the control group, which were significantly lower than those of (8.00 ±5.27)d,(10.33 ±4.81)d,8.33% in the other group (P ＜0.05).Conclusions CBP is an effective method for the patients with severe acute pancreatitis to shorten the duration of mechanical ventilation and the ICU stay,and decrease the mortality of hospital.

Local recurrence and distant metastasis is the major factor of impacting long-term survival for patients with esophageal carcinoma after radical resection. Postoperative radiotherapy and chemotherapy is expected to reduce the rate of local recurrence and distant metastasis, but views for the value of postoperative radiochemotherapy were inconsistent in the past. In recent years, scholars have performed much more clinical studies on postoperative radiotherapy and chemotherapy and found that the combined postoperative radiotherapy and chemotherapy after surgery significantly reduces the recurrence rate and distant metastasis rate and can enhance the survival of the patients.

Objective: To investigate the effects of Zhu Yun Pill(ZYP) in the treatment of infertility due to luteal phase defect(LPD).Methods: 163 infertility patients due to LPD were randomly divided into three groups in terms of ZYP group,progesterone group and combination group(ZYP and progesterone).Then the therapeutic efficacy was judged by the pregnancy rate(PR),endometrial biopsy,the level of serum hormone and basal body temperature before and after the treatment.Result: 40 women were pregnant in ZYP group(cure rate,33.33%),8 in the progesterone group(cure rate,26.67%) and 9 in the combination group(cure rate, 69.23%).The symptomatic amelioration and total effective rate of ZYP group were obviously higher than single exogenous progesterone group(P

Humanism care meets the need of the development of the whole society, the need of transformation of modern medicine pattern it is also the great need for uremia patients who thirst for health.Uremia patients with psychological distress will be greatly improved through following aspects: enhancing quality of medical staff,making more communications with patients and providing health education to them.In a word,humanism care for uremia patients will make them feel more love and improve their living states.

Objective To investigate the expression of S100 and MMP-7 in human cholangiocarcinoma and their correlation with the perineural invasion of cholangiocarcinoma.MethodsThe expression of S100 and MMP-7 in 36 cases of human cholangiocarcinoma tissues and edjunct cancer tissues were analyzed by immunohistochemistry method,combining with the clinical documents analysis.ResultsThe positive rates of S100 and MMP-7 in 36 cases of human cholangiocarcinoma were 61.11%(22/36) and 83.33%(30/36),respectively,while in edjunct cancer tissues were 30.56%(11/36),0.0%(0/36).The positive rate of Hematoxylin and Eosin staining indicated perineural invasion was significantly correlated with S100(P0.05).There was a significant positive correlation between S100 and MMP-7(P

AIM To study the effect of Topotecan(TPT) on cell cycle and apoptosis of hunman lung cancer SPC A 1 cells and the role of Caspase 3 and bcl 2 involved in TPT induced apoptosis. METHODS Cells were incubated with TPT(5,10,15 and 20 mg?L -1 ) for 24 h. Apoptosis was detected by TUNEL staining, electronic microscope and DNA agarose gel electropheresis. Flow cytometer was used to analyse cell cycle changes and to detect apoptotic rates and bcl 2 expression of cells treated with TPT or TPT and 50 ?mol?L -1 Ac DEVD CHO(specific Caspase 3 inhibitor). RESULTS Apoptosis of cells treated with TPT(5 mg?L -1 ) was not detected, but apoptosis rates increased with the increase of TPT concentration and there was significant difference between each concentration group( P

In the hospital administration,the establishment and the implementation of the strategic planning of the hospital occupies a very important position.This paper,combined with the actual situation of a children's hospital in Chongqing,describes the hospital rational use of three level management system of the hospital,office,department,in order to get the full investigation of the national macro policy,the development of the medical industry and the status of the hospital and other aspects,and then supports efficient workflow to complete the hospital strategic planning.This paper also focuses on the target implementation,program implementation and project implementation of three levels,in order to illustrate the process for the implementation of the strategy to provide a reference of.hospital strategic planning formulation and implementation.