From the aspects of advantages, barriers, opportunities and challenges, this paper analyzed the environmental factors of Shanghai medical resources serving people in the Pan-Yangtze River Delta. As envisaged by the author, such services will provide medical resources information to patients from outside Shanghai, probe into feasible ways to medical insurance based on experiences drawn from serving such patients, help with the resources integration of medical systems in Shanghai, and probe into the potentials of telemedicine and distant medical e-learning, as well as improve hospital management levels and service functionality.

Objective To develop a control program running on the magnetic induction escape decompression control timer. Methods The software was developed by VHDL in a modular style under the environment of Quartus II. Results The control software stores decompression profile and accurately controls the commitment of the decompression process. Conclusion The program can work accurately according to the profile selected and meet our needs perfectly.

Objective To investigate the influence of pain on liver function recovery after hepatectomy Methods Clinical data of 257 patients with primary hepatocellular carcinoma were analyzed retrospectively. The patients were divided into effective analgesia group(NRS ≤ 3 points)and ineffective analgesia group(NRS > 3 points)according to the digital hierarchy(NRS). The serum liver function indexes including ALT,AST,TBiL, ALB and PT were compared in 2 groups. Results There was no significant difference in preoperative liver function between 2 groups but the levels of ALT ,AST ,PT and TBIL at 1st ,3rd ,5th and 7th days postoperatively were lower in effective analgesia group than those in ineffective analgesic group after surgery(P<0.05). The incidence of the delay of liver function was lower in effective analgesia group than that in ineffective analgesic group. Conclusion Effective analgesic treatment can reduce liver damage and accelerate liver function recovery.

Objective To investigate the changes of high mobility group box 1 (HMGB1),glutathione peroxidase(GPX3) in thyroid carcinoma and thier relationship with pathological characteristics.Methods The levels of HMGB1 and GPX3 were detected by using ELISA in 153 cases of thyroid cancer,120 cases of benign thyroid nodules and 120 healthy controls.The changes of HMGB1 and GPX3 in the patients with thyroid cancer were analyzed.Their application value in thyroid cancer was analyzed by using the receiver operating characteristic(ROC) curve.Results The plasma HMGB1 level and the HMGB1 positive expression rate in the thyroid cancer group were higher than those in the benign thyroid nodules group and control group(P＜0.05),while the plasma GPX3 level and GPX3 positive expression rate in the thyroid cancer group were lower than those in the benign thyroid nodules group and control group(P＜0.05).There were no significant differences in the levels of plasma HMGB1,GPX3 and the positive rates of HMGB1 and GPX3 between the thyroid benign nodule group and the healthy control group (P ＞ 0.05).The plasma H MGB1 expression in thyroid cancer was closely related with the infiltration degree,clinical stage and lymph node metastasis(P＜ 0.05),and the GPX3 expression was related with the tumor stage and lymph node metastasis(P＜0.05).The ROC curve showed that the sensitivity and specificity of HMGB1 and GPX3 for diagnosing thyroid cancer were 76.3％ and 85.5％,70.8％ and 82.2％ respectively.Conclusion Plasma HMGB1 and GPX3 expression levels are closely associated with the occurrence and progression of thyroid cancer.

Objective To purify rabbit anti-hRBBP10 polyclonal antibody.Methods The recombinant fusion protein PTC-hRBBP10 was expressed in the E.coli and was purified through amylose resin chromatography column and superose 12 gel fitration .The purified PTC-hRBBP10 was coupled to the NHS-activated sepharoseTM to prepare affinity chromatography column to purify rabbit anti-hRBBP10 polyclonal antibody. Results ① PTC-hRBBP10 was expressed and purified successfully with relative molecular mass(Mr) of 80?103 and its purity could reach about 95%.② Purified Rabbit anti-hRBBP10 polyclonal antibody could bind Specifically to PTC-hRBBP10.Conclusions With better specificity, The purified rabbit anti-hRBBP10 polyclonal antibody provide condition for studying the function of the protein, and helps to develop new techniques of tumor diagnosis and treatment.

Objective To prepare rabbit anti-human RBBP10 polyclonal antibody and then character them.Methods New Zealand rabbit was immunized with RBBP10 protein,Polyclonal antiserum was analyzed by ELISA and Western blot.Results The titer of the antiserum obtained in this experiment determined by ELISA was up to 1∶16000 and the sensitivity was at least 10?g/?l.Western blot results show the antibody has high specificity.Conclusions Preparation the Rabbit anti-human RBBP10 Polyclonal antibody in this method is successfully,The expression of the RBBP10 protein in tumor can be detected in immunohistochemistry method,it is useful.

Homoacetogens are a group of microorganisms with application potential to produce chemicals and biofuels by the bioconversion of synthesis gas. In this study, we collected waste activated sludge samples to screen homoacetogens by Hungate anaerobic technique, and studied the effect of pH on acetate and alcohol production from H2/CO2 gas. The mixed culture contained Clostridium ljungdahlii, Lysinibacillus fusiformis and Bacillus cereus. Acetate concentration achieved 31.69 mmol/L when the initial pH was 7. The mixed culture containing homoacetogen could converting H2/CO2 to acetate, which provides an efficient microbial resource for the bioconversion of synthesis gas.
Acetates ; chemistry ; Bacteria ; classification ; Biofuels ; Carbon Dioxide ; Hydrogen ; Sewage ; microbiology

[Objective]To investigate the prophylactic effects of intra-articular injection of salvia miltiorrhiza on joint adhesion. [Methods]Twenty-four Japanese male rabbits were randomly divided into 3 groups.All right hind knees were made in this way: the auterior wall of suprapatellar bursae was scraped, the posterior wall of suprapatellar bursae and the infrapatellar fat pad were cut off. Each knee was injected with physiological saline 0.3 ml for group A, sodium hyaluronate injection 0.3 ml for group B, and salvia miltiorrhiza injection 0.3 ml for group C. All right hind knees were immobilized in the extension position with a long leg plaster cast for 4 weeks, and injected weekly.[Results]After 4 weeks, the joint movement range in group B or C was larger than that in group A (P<0.01), and there was no significant difference (P>0.05)compared to group B and group C. The scoring of joint adhesions in group B or C was smaller than that in group A (P<0.01), and there was no significant difference(P>0.05)compared to group B and group C. The scoring of histological changed of the synovial membrane in group B or C was smaller than that in group A (P<0.01), and there was no significant difference(P>0.05)compared to group B and group C.Total collagens content of the synovial membrane in group B or C was smaller than that in group A (P<0.01), while that of group C was less than in group B(P<0.01). [Conclusion]Similarly to sodium hyaluronate, salvia miltiorrhiza can effectively prevent joint adhesions.

Objective:To investigate the effects of pregnancy and lactation nonylphenol (NP) exposure on the balance of Treg/Th17 cells in the brain of offspring mice and the related mechanisms.Methods:Thirty pregnant C57BL/6 mice were randomly divided into three groups: control group (drinking distilled water), and NP-treated groups (drinking 0.2 μg/ml or 2.0 μg/ml NP water solution). ELISA kit was used to analyze the levels of TNF-α, IFN-γ and IL-17, flow cytometry was used to analyze the frequency of Treg and Th17 cells in spleen, quantitative RT-PCR was used to analyze the RORγt, Foxp3 mRNA, Western blot was used to analyze the protein expression of RORγt, Foxp3 and PI3K/Akt/mTOR signal pathway, and immunofluorescence was used to analyze the expression of Iba1 in the brain tissue of offspring mice.Results:Compared with the control group, NP exposure increased the serum levels of IL-17 and TNF-α in male offspring mice ( P<0.05), and decreased the levels of IFN-γ( P<0.05). Flow cytometry analysis showed that the percentage of Th17 cells in the spleen of male offspring mice exposed to NP (0.2 μg/ml or 2.0 μg/ml) was significantly higher than that of the control group, while the percentage of Tregs cells was lower. Compared with the control group, the expression levels of Foxp3 proteins in the brain tissue of male offspring mice exposed to NP (0.2 μg/ml or 2.0 μg/ml) was significantly lower, accompanied by a dramatic increase in RORγt protein levels ( P<0.05). Similar mRNA expression was also observed in qRT-PCR analysis. The protein expression levels of mTOR (p-mTOR) and its upstream related regulators[PI3K, p-Akt (Ser473), p-Akt (Thr308)] in the brain of male offspring mice increased gradually during the period of exposure to NP( P<0.05). Immunofluorescence analysis showed that compared with the control group, the number of Iba1 positive cells in brain tissue of male offspring mice exposed to NP (0.2 μg/ml or 2.0 μg/ml) increased significantly ( P<0.05). Conclusions:Maternal exposure to NP during pregnancy and lactation may affect the development/function of neurons in offspring through neuroimmune axis, and increase the risk of neurodevelopmental disorders in offspring.

Objective:Dysmenorrhea is the common gynecological problem among adolescent girls and women of reproductive age. This study aim to develop a dysmenorrhea Quality of Life (QoL) scale based on Traditional Chinese Medicine (TCM) theory.Methods:We conducted focus group discussions and in-depth interviews with TCM gynecologists and patients, and adapted items from previously published scales. We generated an initial pool of 41 items with 8 domains. Delphi method was used to item preliminary selection. Then, we administered the items to a sample of adolescent girls ( n=200), and the distribution of survey items, discrete trend, factor analysis, correlation coefficient, Cronbach’s α coefficient were used to select items. Results:After two rounds of Delphi, a total of thirty items were included in the dysmenorrhea QoL scale. The expert positive coefficient were 100% and 83.3% with high motivation. The authoritative coefficient were greater than 0.7, the results showing authoritative and reliable. The Kendall’s coefficient of concordance W was 0.333 ( χ2=128.271, P<0.001). In sample analysis, the items were deleted when they met more than two standards. The final scale retained 20 items, covering 8 dimensions. Conclusions:The methods for selection of dysmenorrhea QoL scale based on TCM theory were preferable. Given the paucity of research in this area, this new dysmenorrhea QoL scale may provide opportunities for the patient-reported outcome (PRO) evaluation in the field of TCM.