0.05). Conclusions The LTs synthesis is elevated in RSV bronchiolitis and LTs may participate in the pathogenesis of RSV bronchiolitis. Leukotriene receptor antagonist may play an important role in RSV bronchiolitis. However there still needs further research on the relationship between LTs and eosinophils count in the pathogenesis of RSV bronchiolitis.

Objective To observe the effect of self mading Tongfengxiao decoction in treatment of acute gouty arthritis. Methods 69 patients were randomly divided into two groups. In the control group,33 patients were given diclofenac sodium sustained-release capsules and allopurinol treatment;In treatment group ,36 patients were given to the self mading Tongfengxiao decoction in addition to the western medicine received by the control group. The two groups were all treated for 14 days. Results The total effective rate was 94.4% for treatment group and 87.8% for control group respectively. Therefore, the difference of treatment effect between twp groups was significant ( P ＜0. 05 ) ;Also, the treatment group performed better than control group in term of reducing serum uric acid( P ＜ 0. 05).Conclusion The treatment of Tongfengxiao decoction combined western medicine for acute gouty arthritis had a good synergistic effect, and could reduce serum uric acid and improve clinical symptoms.

Objective To determine the role of C-reactive protein(CRP) in evaluating the activity of ulcerative colitis. Methods Analysis was used to estimate the correlation of CRP with mean platelet volume in 41 patients with active ulcerative colitis and 17 patients ulcerative colitis in re-mission. The effects on disease severity were analyzed subsequently. Results Active UC patients sera CRP level(8.58 ±2.43)mg/L,which level of severe UC CRP (14.38 ±4.46)mg/L,the moderate level of CRP UC(6.68 ±2.35)mg/L,mild level of CRP UC(4.94 ±1.21)mg/L, while the remission stage UC patients CRP levels(3.05 ±0. 88)mg/L. CRP was found to be significantly higher in sera of patients with active ulcerative colitis than that in patients in remission (P ＜ 0.05). The sera level of CRP in inflammatory bowel disease at severe stage were significantly higher than those of medium stage (P ＜ 0. 05). CRP was observed to be slightly increased in sera of patients at mild stage (P ＜ 0. 05). Conclusion The increase of CRP could reflect the severity of active ulcerative colitis.

Objective To explore the effect of gamma-aminobutyric acid (GABA) on the growth, apoptosis and telomerase activity of Cholangiocarcinoma cell line QBC939. Methods Cholangiocarcinoma cell line QBC939 was cultured by routine method, and then treated with different concentrations of GABA (1-1000 μmmol/L). The proliferation, apoptosis and cell cycle of QBC939 cells was investigated by MTT, Flow cytometry and transmission electron microscopy. The telomer-ase activity of QBC939 cells was examined by modified PCR-ELISA assay. Radioimmunoassay was used to measure the intracellular cyclic adenosine monophosphate(cAMP) content. Results The dif-ferent concentrations of GABA inhibited the growth of QBC939 cells and promoted the apoptosis. The apop-totic rate of QBC939 cells was increased from 4. 8% to 28. 03%, which had significant difference (P<0.05). It had no effect for distribution of cell cycle. Cell nuclear condensation and apoptotic bodies were seen by transmission electron microscopy. Telomerase activity was inhibited by GABA(0. 82±0. 048 vs 0.56±0. 054, P<0.05). The content of intracellular cAMP was increased with the increase of GABA concentration in a dose-dependent manner [(0. 59±0. 049) nmol/L vs (0. 82±0. 033)nmol/L, P<0. 05]. Conclusion GABA can inhibit the proliferation of QBC939 cells by promoting apoptosis and inhibiting telomerase activi-ty, which may be mediated by the information transmission of post-receptor.

Objective To investigate the effect of nuclear factor-kappa B(NF-κB) on the expression of parathyroid hormone receptor (PTH1R) and serum calcium level in rats with acute necrotizing pancreatitis (ANP) rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham operation group (SO), ANP group, pyrrolidine dithiocarbamat (PDTC, an inhibitor of NF-κB) pretreated ANP group (PDTC), with 8 rats each group. ANP model was induced by injecting 5% sodium taurodeoxyebolate into biliary pancreatic duct. Serum calcium was measured and the expression of NF-κB was determined by western blot and the expression of PTH1R was examined by RT-PCR plus western blot 6 h after ANP induction. Results The NF-κB proteins of bone and kidney tissues in ANP group were 1.53±0.08 and 0.47±0.19, respectively; in PDTC group they were 0.61±0.13 and 0.36±0.06 with significant difference (P<0.05). The PTH1R mRNA expressions of bone and kidney in ANP group were 0.27±0.08 and 0.29±0.06, respectively; the PTH1R proteins expressions were 0.87±0.07 and 0.31±0.09, respectively. The corresponding values in PDTC group were 0.57±0.27 and 0.37±0.11, 1.13±0.17 and 0.68±0.15, respectively, with significant difference (P<0.05). The activity of NF-κB increased in ANP group, the expressions of PTHI R decreased significantly. Compared with ANP group, the activity of NF-κB decreased and the levels of PTH1R and serum calcium increased in PDTC group (P<0.05). Conclusions NF-κB may indirectly down-regulate the expression of PTH1R, which resulting into the hypocalcaemia of ANP. PDTC may alleviate hypocalcaemia in ANP.

Objective To investigation the relationship between health management and prognosis of chronic prostatitis. Methods Three hundied and forty-six cases of chronic prostatitis were divided into two groups. The control group treatment was local washing and medicine for three months. The experiment group treatment was local washing, medicine, and health management for three months. NIH-CPSI was used for the results. Results There was no significant different between the experimental group(165 cases)and the control group(154 cases)before treatment in NIH-CPSI. The effective rate of the the experimental group was 88.5%, and that of the control group was 64.9% after treatment. It is effective for cure and the prognosis of the experiment was better than the control (P<0.05). Conclusion It is suggested that it is more effective for the prognosis of chronic prostatitis after the intervention of health management.

In the tumor microenvironment, inflammatory cells regulate intracellular signaling transduction mediated by the corresponding nuclear transcription factor by releasing inflammatory factors ,which leads to transformation of epithelial cells to mesenchymalcellsand eventually promote tumor metastasis. Because of the intimate relationship between inflammation and tumor metastasis , it would be helpful for further enhancement of effectiveness of tumor therapy if we could better understand both the biological character of tumor and the related inflammatory factors.

AIM To investigate if scallop (Placopecta magellanicus) skirt glycosaminoglycan (SS-GAG) inhibits the proliferation of vascular smooth muscle cell (VSMC) as heparin does so and to clarify its mechanism. METHODS The inhibitory effects of SS-GAG on the proliferation of rat thoracic aorta and abdominal aorta VSMC induced by fetal bovine serum (FBS) or basic fibroblast growth factor (bFGF) were determined by cell counting, crystal violet staining and MTT colorimetry. The effects of SS-GAG on the expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor (PDGF) in VSMC proliferation induced by bFGF were evaluated by immunohistochemical technique (LSAB method) and computer image analysis system. RESULTSSS-GAG exerted antagonistic effects on VSMC proliferation induced by 20% FBS and 50 μg·L-1 bFGF at concentrations ranging from 50 mg·L-1 to 200 mg·L-1 and repressed the increasing expression of PCNA and PDGF. CONCLUSION SS-GAG significantly inhibits the proliferation of VSMC, which may be carried out through repression of PDGF and PCNA expression.

Objective　To evaluate the effect of inducing differenation of all trans retinoic acid (ATRA) on immunologic function of patients with gastric cancer. Methods　T-lymphocyte subsets(T-Ls) and interleukin-2 receptor(sIL-2R) of 56 patients with gastric cancer after treatment of ATRA were studied. Results　In radical gastric cancer resection group, the serum CD3,CD4 and CD4/CD8 rate were higher and sIL-2R were lower than those in the control group, after treatment of ATRA, CD3,CD4 and CD4/CD8 rate and sIL-2R were as high as those in the control group. In the non-operative or palliative gastric resection group, CD3,CD4 and CD4/CD8 rates were increased markedly and the serum sIL-2R was decreased significantly than those in the control group. Conclusion　ATRA inducing differenation can improve the immunity of the patients with gastric cancer.

The factors influencing the integration of MOOC and information literacy education in academic libraries were divided into objective factor, subjective factor and social factor according to their connotation and significance. Certain measures for their integration were proposed, such as strengthening network construction, perfecting literacy education systems, and carrying out systemic training.Integration of MOOC and information literacy education in academic libraries can improve information service, promote wide application of MOOC and information literacy ed-ucation in academic libraries.