Objective:To establish the methods for the quality evaluation and hesperidin content determination of Radix Zanm-thoxyli Avicennae. Methods: The moisture, ash and extract content was respectively detected according to the methods described in Chinese Pharmacopoeia 2010 edition, and the content of hesperidin was detected by HPLC. Results: The moisture contents of Radix Zanmthoxyli Avicennae from seven different areas were between 7. 27% and 9. 65%; the total ash contents were between 5. 28% and 7. 90%;the acid-insoluble ash content were between 0. 39% and 0. 86%;the water-soluble extract contents were within the range of 5. 95%-10. 72% by infusion and 8. 16%-12. 67% by hot dipping;the alcohol extract contents were within 5. 88%-10. 97% by infu-sion and 2. 38%-3. 83% by hot dipping. The content of hesperidin was 0. 40%-2. 68%. Conclusion:The established methods can be used to evaluate the quality of Radix Zanmthoxyli Avicennae.

Objective To investigate the significance of peroxisome proliferator-activated receptor (PPAR)γexpression in the lung tissue of rats with chronic hypoxic pulmonary hypertension (HPAH). Methods Forty male Sprague-Dawley rats were randomly divided into four groups (n=10 for each group):normal control group (NC), hypoxia control group-one-week (HC-1w), hypoxia control group-two-week (HC-2w) and hypoxia control group-three-week (HC-3w). Normal control group was raised under normal oxygen condition in ventilated animal cage for three weeks. The other HC groups were placed in a low oxygen chamber (O2 concentration of 10%) from 9:00 AM-5:00 PM (8 h/d) everyday by one week, two weeks and three weeks. The mean pulmonary arterial pressure (mPAP), right ventricular systolic pressure (RVSP) were detected. The index of right ventricular hypertrophy RV/(LV+S) was measured by dissecting rat heart. The morphological changes of the small pul-monary arteries were observed by HE staining, and the percentage of vascular wall thickness (WT%) was calculated. The ex-pression level of PPARγprotein was detected by Westren blot assay. Results The mPAP, RVSP and RV/(LV+S) were sig-nificantly higher in HC groups than those of NC group (P<0.05). The morphology of pulmonary arteries showed vessel wall thickening and vessel lumina stenosis in HC groups compared with that of NC group. The PPARγexpression in lung tissue was significantly lower in HC groups than that of NC group, and the downward trend was more obvious with the extension of time. Conclusion PPARγplays an important role in the occurrence and development of chronic hypoxic pulmonary hyper-tension.

DEK protein is an abundant chromatin protein in metazoans with highly conserved nuclear factor. This protein is a unique member of its family and is preferentially expressed in actively proliferating and malignant cells. Recently, much attention has been paid to the role of DEK protein in the development of various cancers, which was originally discovered in a subset of acute my-elogenous leukemia. Oncogene DEK is overexpressed in several malignancies including hepatocellular carcinoma, glioblastoma, retino-blastoma, bladder cancer, malignant melanoma, and cervical cancer. Oncogene DEK is a chromatin remodeling protein that supports cancer cell proliferation and invasion. In this review, we summarized research advancements in the correlation between DEK protein and oncogenesis.

Objective To investigate the effects of pyrrolidine dithiocarbamate (PDTC) on plasma concentrations of proinflammatory cytokines and myocardial energy metabolism induced by cardiopulmonary bypass (CPB) .Methods Twelve healthy mongrel dogs of both sexes weighing 13.5-17.5 kg were randomly divided into 2 groups (n = 6 in each group) : PDTC group and control group. The animals were anesthetized with intraperitoneal 2.5% pentobarbital sodium 25 mg?kg-1, intubated and mechanically ventilated. In PDTC group PDTC 30 mg?kg-1 was given i.v. after tracheal intubation while in control group normal saline was given instead of PDTC. Aorta was clamped for 60 min and then undamped for 60 min reperfusion during CPB. Blood samples were taken before (baseline), 30 and 60 min after aortic clamping and 30 and 60 min after aortic unclamping for determination of plasma concentrations of TNF-? and IL-1?. Myocardial tissue was obtained before and 60 min after aortic clamping and 60 min after aortic unclamping for determination of myocardial content of adenine nucleotide (ATP, ADP, AMP, TAN, EC) and expression of ICAM-1 protein.Results Plasma TNF-? concentration was increased after aortic cross-clamping as compared to the baseline value before clamping in both groups but the TNF-? concentration was significantly lower in PDTC group than in control group (P

lymorphism of CD14 gene is a risk factor for diabetic nephropathy.

This paper reported a rapid hydrolysis method of food protein using microwave oven and high pressure vessel technique. Tryptophan in hydroly-zate was determined by fluorimetric spectroscopy method. The hydrolytic agent was 5 M NaOH solution. Under the operative condition of the microwave oven (microwave output power 650W, 2 minutes 15 seconds and 65W 2 minutes) the highest tryptophan contents of the pure protein lysozymum and food SRM liver, cabbage and wheat flour were obtained. The results of the t-test showed that there was no significant difference between the tryptophan contents determined by using the operative condition of the microwave oven mentioned above and 145℃ 4h hydrolysis method. The recovery of the method was 93.3%-107%, and the coefficient of variation obtained by parallel determination of 10 lysozymum sample was 4.2%.

OBJECTIVE: To analyze the chemical constituents of essential oil of Acorus calamus from different habitats in order to provide scientific basis of exploitation of A.calamus.METHODS: The essential oil was extracted from A.calamus by steam distillation.The chemical constituents were analyzed by GC-MS.The relative contents of chemical constituents were calculated using normalization method.RESULTS: 39 kinds of chemical components were identified in A.calamus from Yunnan province,accounting for 80.51% of total amount of essential oil.37 kinds of chemical components were identified in A.calamus from Sichuan province,accounting for 81.96% of total amount of essential oil.There were 31 kinds of same chemical constituents in A.calamus from Yunnan province and Sichuan province.The content of ?-asarone was highest in A.calamus from Yunnan province (13.46%) and that of ?-asarone was highest in A.calamus from Sichuan province (10.01%).CONCLUSION:The study can provide scientific basis for the further development of A.calamus.

Objective:To explore the diagnostic value of MRI in the diagnosis ofbone Eosinophilic GranulomaI. Methods:The clinical and imaging materials of 13 patients with eosinophilic granuloma of bone proved by histopathology were analyzed retrospectively.The imaging examination included plain films(n=13),CT(n=12),and MRI(n=13).Results:MR identified all lesions;With one exception,all lesions were hypointense on T1-Weighted images and hyperintense on T2-Weighted images;The lesions and associated soft tissue abnormalities were very conspicuous on short T1 inversion sequences and T1-Weighed post-contrast images;Follow-up MRI studies in two patients after chemotherapy showed decreased size and enhancement of lesions compared with the baseline studies.Three lesions were not identified on plain films.MRI showed greater abnormality than plain radiographs did.CT could identify all lesions also,and the main imaging findings included bone destruction,adjacent bone cortex involvement,periosteal reaction and sof t tissue mass or swelling. Conclusion:MRI can clearly demonstrate the extent of bone involved, the change of soft tissue and sequestrum.It can also acquire the anatomic and pathologicd details of bone and and soft tissue.But X-ray film and CT may improve the diagnostic and differential diagnostic accuracy.

Objective:To compare the immunizing efficiency in mice with recombinant adenovirus inoculated by intranasal, intramuscular or oral routes.Methods:BALB/c mice were immunized with 108 PFU recombinant adenovirus expressing rotavirus VP4 antigen intranasally, intramuscularly or orally.The mice were boosted twice with the same dose by the same route. Serum, stool and intestine specimens were collected to detect rotavirus VP4 specific antibodies by ELISA. Mice were mock treated with adenoviral vector and PBS as the blank control.Results:Inoculation with the recombinant adenovirus by these 3 routes elicted rotavirus VP4 specific serum and intestinal antibodies(P

Total saponins in crude drug and induced callus of Gynostemma pentaphyllum (Thunb. )Mak. grown in Jiangxi Province were analysed by spectrophotometry and TLC. Results sh-owed that the quality and quantity of saponins in crude drugs valicd collsiderably with cu-lture area, the life cycle and their crgans. The quality and quantity of saponins in callusalso varied due to source of clone. It providcd some cvidence for the production and harv-est of G. pentaphyllum.