Objective To investigate the effect of Platelet-activating factor(PAF)in pathogenesy of asthma in high altitude Methods ELISA and other methods were used to detect the quantity of platelet(PLT)and the level of serum platelet-activating factor(PAF)in the plain control group、the plateau control group and the plateau asthma patients group.Results The quantity of PLT in plain control group and plateau control group were significantly lower than that of plateau asthma patients group.(P

ObjectiveA method was proposed for the determination of the concent of total glucoside of peaony(TGP) fromPaeonia lactifloriaPall. by UV spectrophotometry.MethodsBased on the extract with 80% acidities alcohol, extracted by aether and n-butyl alcohol, TGP was obtained fromPaeonialactifloriaPall.. The determination was established with paeoniflorin which was the main component of TGP as a contrast by UV spectrophotometry at 230 nm.ResultsA good liner relationship between concentrationof paeoniflorin and its absorbance was obtained,and the liner range was between 0.040 2~0.201 1 mg/ml. The averagerecovery was 101.81%.ConclusionThis method is simple rapid and reliable. It could be used for the semi-quantitative determination of TGP of paeony in paeonialactifloria pall..

Objective To investigate the influencing factors of medication compliance of glucocorticoid for patients with interstitial lung disease (ILD). Method The outline in-depth interviews with 15 patients with ILD was done using content analysis method, and the influence factors of patients medication compliance were analyzed. Result The influence factors of medication compliance in ILD patients can be summarized as the following three themes: patients′individual factors, factors related to medical and absence of social and family support. Conclusion Nurses should master the influencing factors of hormone medication adherence in ILD patients, ready to take appropriate intervention measures, improve the medication compliance in patients with ILD hormone and promote their rehabilitation.

Combined Modality Therapy ; methods ; trends ; Heart Defects, Congenital ; therapy ; Humans

Adult ; Drug Resistance, Viral ; genetics ; Female ; Genotype ; Guanine ; analogs & derivatives ; pharmacology ; therapeutic use ; Hepatitis B virus ; drug effects ; genetics ; Hepatitis B, Chronic ; drug therapy ; genetics ; Humans

Amikacin ; adverse effects ; Anti-Bacterial Agents ; adverse effects ; Child ; Drug Eruptions ; etiology ; Erythema Multiforme ; chemically induced ; Humans ; Male

Objective:To investigate the compatibility stability of muscular amino acids and peptides and nucleosides for injection in different infusions to provide basis for clinical application .Methods: The compatibility stability of muscular amino acids and pep-tides and nucleosides for injection respectively in 0.9%sodium chloride injection , 5% glucose injection , 10%glucose injection and glucose and sodium chloride injection was studied , and the indices included the appearance , pH value , number of insoluble particles and contents of hypoxanthine and polypeptides .Results:All the solutions were transparent .The pH value and the contents of hypoxan-thine and polypeptides showed no significant changes .When muscular amino acids and peptides and nucleosides for injection was mixed with 10%glucose injection , the number of insoluble particles (≥10 μm) was the smallest , which met the requirement in Chi-nese pharmacopoeia (2015 edition,volume Ⅳ).When it was mixed with 0.9% sodium chloride injection, 5% glucose injection and glucose and sodium chloride injection , the number of insoluble particles (≥10 μm) was beyond the limits .The number of insoluble particles (≥25 μm) in all the solutions met the requirement .Conclusion: The most suitable solvent for muscular amino acids and peptides and nucleosides for injection is 10%glucose injection .

Objective To explore the nervous mechanism of intestinal function disorder after intestine congestion through observing the changes of acetylcholinesterase (AchE) neuron and nitric oxide synthase neuron (NOS) expression in nervous system of rat intestinal tract. Methods Sprague Dawley rats were divided into experimental groups (intestine congestion 20min and 60min group) and the control group. Then the spreading specimens of intestinal myen-teric plexus of ileums were collected and stained by AchE and nicotinamide-adenine dinucleotide phosphate-D (NADPH-d) histochemistry, to observe and compare the density of distribution and staining of AchE and NOS positive neurons. Result Compared to the control group, the number and positive expression of AchE positive neuron in intestinal myen-teric plexus of ileums in the experimental group rats decreased (P

Objective:To investigate the levels of serum leptin and their effect on Th 1/Th2 balance and the association between serum leptin levels and clinical manifestations in obese children with asthma.Methods: All 56 children with physician-diagnosed moderate-to-severe persistent asthma and 56 healthy controls were included.The children with asthma and without asthma were divided into two groups,obese (n=28) and non-obese (n=28) by body mass index,respectively.Asthma severity was assessed by the asthma symptom score.Peripheral blood samples were collected to determine the levels of leptin , IFN-gamma and IL-4.The results were correlated with the parameters studied.Results:Serum leptin levels were significantly higher in the obese asthma group than in the non -obese asthma group ,as well as being significantly higher in the children with asthma than in the controls ( P<0.01 ) , whereas IFN-gamma levels were significantly higher and IL-4 levels were significantly lower in the obese asthma group than in the non-obese asthma group( P<0.01 ).In addition, the obese asthma group showed higher asthma symptom scores and significantly lower FEV 1 (% of predicted) than did the non-obese asthma group(P<0.01).There was a significantly positive correlation between leptin ,IFN-gamma and IL-4 levels only in the obese asthma group ( P<0.01 ).Conclusion:Leptin is involved in the pathogenesis of asthma in obese and non-obese children ,and its effect is more pronounced in the former.In the presence of high leptin levels , only obese children with asthma exhibited Th1 polarization,with higher IFN-gamma levels and greater asthma severity.

AIM:To investigate the effects of fucoidan on the angiogenesis of multiple myeloma cells in vitro, and its related mechanisms .METHODS:The human multiple myeloma RPMI 8226 cells and human endothelial cells were cultured in vitro.The growth inhibition rate of RPMI 8226 cells was examined by MTT assay .The cell cycle and apoptosis rate were measured by flow cytometry .RPMI 8226 cells were treated with fucoidan for 72 h, and the cell culture superna-tant was collected .The VEGF concentration was examined by ELISA , and the tube formation assay was applied to assess the angiogenic activity .After treatment with fucoidan for 72 h at different concentrations , the protein levels of HIF-1α, VEGF, p-AKT and p-ERK1/2 were detected by Western blot .RESULTS: Fucoidan inhibited the growth of RPMI 8226 cells in a dose-and time-dependent manner .After treatment with fucoidan for 72 h, the cell cycle was arrested at G 1 phase , and the apoptotic rate of RPMI 8226 cells was increased with the increasing concentration of fucoidan , which was much higher than that in control group (P<0.05).The VEGF concentration was significantly decreased with the increa-sing concentration of fucoidan .The numbers and areas of the capillary-like structures decreased while the concentration of fucoidan increased, and those at 100 mg/L were less than those in the control (P<0.05).The protein levels of HIF-1α, VEGF, p-AKT and p-ERK1/2 in fucoidan group were significantly lower than those in control group (P<0.05).CON-CLUSION:Fucoidan inhibits the secretion of VEGF in multiple myeloma cells , and reduces angiogenesis induced by mul-tiple myeloma cells .It inhibits the protein expression of HIF-1αand VEGF , which may be related to inhibiting the phos-phorylation of AKT and ERK1/2.