In order to investigate the growth of in vitro cultured microencapsulated adre nal cortical tissues of rats, we produced the microcapsules by using sodium alginate, calcium chloride and poly-L-lysine, etc. The adrenal glands were taken out of 24 rats, half of the adrenal glands were microencap sulated (capsule group) while the other half were left free (noncapsule group). After 36 hours' in vitro culture, the concentrations of aldosterone and cortisol in culture solution were measured by radioimmunoassay. The two groups con tinued to be cultured for 24 and 36 hours with ACTH stimulation. The concentrations of aldosterone and cortisol at 24 and 36 hours were detected respectively. Parts of adrenal cortical tissues were observed under light microscope and electron micros cope. The results showed that the concentrations of aldoste rone and cortisol in the microencapsulated adrenal cortical tissues were signifi cantly higher than those of noncapsule group (P＜0.01). In the capsule group , the concentration of cortisol after 36 hours' ACTH stimulation was significantly higher than that before ACTH stimulation (P＜0.01). After 24 and 36 hours' ACTH stimulation, the concentrations of aldosterone and cortisol were sign if icantly higher in the capsule group than in the noncapsule group. In the nonca psule group, regardless of ACTH stimulation, no significant change was seen in the concentration of aldosterone and cortisol (P＞0.05). The cellular structure of adrenal tissues remained intact under light microscopic and electron microscopic observations, and the cells remained alive well. These data suggest that in vitro cultured cells of microenc ap sulated adrenal cortical tissues remain alive well. Microcapsule has not influen ced the secretory function of adrenal tissues. Microencapsulated tissues have a good response to ACTH stimulation.

Objective:Asthma sufferers exhibit high prevalence of anxiety/depression.Elevated tumor-necrosis factor-alpha (TNF-α) levels in peripheral system and central nervous system (CNS) are associated with anxiety/depression,whereas brain-derived neurotropic factor(BDNF) has anti-depressant effects.An anti-asthma herbal medicine intervention ASHMI inhibits peripheral TNF-α secretion in an animal model of asthma.We hypothesize that ASHMI and its compounds may have modulatory effects on CNS TNF-α and BDNF production.We sought to determine the effect of ASHMI and individual herb constituents on brain microglial cell TNF-α production,and identify the active compounds that suppress TNF-α and increase BDNF.Methods:BV-2 mouse microglial cells were pre-treated with ASHMI or extracts of Ganoderma lu-cidum (G.lucidum),Sophora flavescens Ait (S.flavescens),and Glycyrrhiza uralensis Fischer (G.uralensis),the herbal constituents in ASHMI,or individual compounds isolated from G.uralensis at different concentrations and then stimulated with LPS.TNF-α levels in culture supernatants were measured by ELISA.The effect of active compounds on NFκB signaling pathway and on BDNF production were determined by western blotting and ELISA,respectively.Results:ASHMI produced dose-dependent inhibition of TNF-α secretion by cultured-mouse micro-glia BV2 cells.Of the three herb extracts in ASHMI,only G.uralensis significantly and dose-dependently inhibited TNF-α production.Among the 5 flavonoids isolated from G.uralensis,isoliquiritigenin was the most effective.Isoliquiritigenin suppression of TNF-α production was asso-ciated with attenuation of p-NF-κB expression,and was accompanied by increased BDNF secretion.Conclusion:ASHMI and its effective flavonoid,isoliquiritigenin,inhibited TNF-α production by LPS stimulated microglial cells and elevated BDNF levels,which may prove to have anti-CNS inflamma-tory and anti-anxiety effects.

OBJECTIVE: To investigate the early effectiveness of transiliac-transsacral screws internal fixation assisted by augmented reality navigation system HoloSight (hereinafter referred to as "computer navigation system") in the treatment of posterior pelvic ring injuries. METHODS: A retrospective analysis was made in the 41 patients with posterior pelvic ring injuries who had been treated surgically with transiliac-transsacral screws between June 2022 and June 2023. The patients were divided into navigation group (18 cases, using computer navigation system to assist screw implantation) and freehand group (23 cases, using C-arm X-ray fluoroscopy to guide screw implantation) according to the different methods of transiliac-transsacral screws placement. There was no significant difference in gender, age, body mass index, causes of injuries, Tile classification of pelvic fracture, days from injury to operation, usage of unlocking closed reduction technique between the two groups ( P>0.05). The time of screw implantation, the fluoroscopy times, the guide wire adjustment times of each screw, and the incidence of complications were recorded and compared between the two groups. The position of the transiliac-transsacral screw was scanned by CT within 2 days after operation, and the position of the screw was classified according to Gras standard. RESULTS: The operation was successfully completed in both groups. The time of screw implantation, the fluoroscopy times, and the guide wire adjustment times of each screw in the navigation group were significantly less than those in the freehand group ( P<0.05). There were 2 cases of incision infection in the freehand group, and the incision healed by first intention after active dressing change; there was no screw-related complication in the navigation group during operation and early period after operation; the difference in incidence of complications between the two groups (8.7% vs. 0) was not significant ( P=0.495). According to the Gras standard, the screw position of the navigation group was significantly better than that of the freehand group ( P<0.05). CONCLUSION Compared with the traditional freehand method, the computer navigation system assisted transiliac-transsacral screws internal fixation in the treatment of posterior pelvic ring injuries has advantages of improving the accuracy of screw implantation and reducing radiation damage and the time of screw implantation.
Humans ; Retrospective Studies ; Surgical Wound Infection ; Replantation ; Body Mass Index