No abstract available.
Humans ; Listeria* ; Meningitis, Listeria*

No abstract available.
Listeria* ; Meningitis, Listeria* ; Transplantation*

No abstract available.
Listeria monocytogenes* ; Listeria* ; Macrophages* ; Salmonella typhimurium* ; Salmonella*

Listeria momocytogenes is a positive gram bacillus, not producing odontoblast, often seen in soil, straw and grass and plants. Listeria can infect to herbs, cooked food and cheese and paste in fridge. Listeria gets in human body by digestion of contaminated food. The disease caused by listeria is dangerous with the mortality rate can up to 30% of cases. To prevent listeria-caused diseases, food safety has to be promoted, especially for people with exposure (infants, pregnant women, immune-compromised people). Biochemical tests to differentiate Listeria momocytogenes from other forms of non-harm listeria variations help diagnose at the earliest possible stage.
Food ; Listeria Infections ; Diagnosis

No abstract available.
Animals ; Listeria* ; Listeriosis* ; Mice*

No abstract available.
Arthritis ; Bacteremia* ; Female* ; Humans ; Knee Joint* ; Knee* ; Listeria monocytogenes* ; Listeria*

No abstract available.
Child* ; Humans ; Listeria monocytogenes ; Listeria* ; Listeriosis* ; Meningitis ; Sepsis

Aims: This study was designed to evaluate the effectiveness of the synthesised carvacrol loaded chitosan nanoparticles (CLCNPs) on the growing and pre-formed biofilms of Listeria monocytogenes isolated from slaughterhouses. Methodology and results: The swab samples were collected from knives, hocks and cutting tables representing slaughterhouses meat contact surfaces (MCS), while those samples from walls and floors represent slaughterhouses meat non-contact surfaces (MNCS). The bacteriological analysis revealed the existence of L. monocytogenes with a prevalence rate of 3.3, 10 and 6.7% for knives, hocks and cutting tables, respectively and 2.2 and 6.6% for walls and floors, respectively. The isolates L. monocytogenes were assayed for biofilm production by the crystal violet binding assay method. Among the 10 L. monocytogenes isolates, 10%, 50% and 30% of the isolates were found to be strong, moderate and weak biofilm producers, respectively. The activities of carvacrol, chitosan nanoparticles (NPs) and CLCNPs against the only strong biofilm producer strain of L. monocytogenes were tested by microtiter plate assay. The minimum inhibitory concentrations (MIC) values were 3.75 mg/mL for CAR, 5 mg/mL for chitosan NPs and 0.62 mg/mL for CLCNPs. CLCNPs inhibit the produced biofilm by 35.79, 73.37 and 77.76%, when 0.5 MIC, 1 MIC and 2 MIC were used, respectively. Furthermore, the pre-formed L. monocytogenes biofilms were significantly reduced from 1.01 (control) OD570 to 0.40 and 0.29 OD570 by applying 2 MIC and 4 MIC doses, respectively. Conclusion, significance and impact of study The data generated is promising to develop bio-green disinfectants to inhibit biofilm formation by L. monocytogenes in the food processing environment and control its adverse effects for consumers.
Chitosan--chemistry ; Listeria monocytogenes ; Nanoparticles

Listeriosis in a healthy child is extremely rare. A previously healthy 9-year-old male was admitted with pyrexia, headache and lethargy. The CSF cultures, taken on admission and on the 5th day, showed growth of Listeria monocytogenes. L. monocytogenes was identified by various methods, including polymerase chain reaction(PCR). Serologically, it showed type 1/2a. However, blood cultures, which were taken on the same days, revealed no growth of L. monocytogenes. Ceftriaxone and amikacin had no effect on the progress of the disease. The combination of ampicillin plus gentamicin, which is regarded as the treatment of choice for L. monocytogenes infection, had an effect in this case. Patient showed severe neurological symptoms and signs, such as diplopia, esotropia, nystagmus, ptosis and other meningeal signs during the hospital days, even though all such symptoms and signs were improved at discharge. We report the case with brief review of related literatures.
Amikacin ; Ampicillin ; Ceftriaxone ; Child* ; Diplopia ; Esotropia ; Fever ; Gentamicins ; Headache ; Humans ; Lethargy ; Listeria monocytogenes ; Listeria* ; Listeriosis ; Male ; Meningitis, Listeria*

Aims: This study aims to isolate lactic acid bacteria (LAB) from various food sources to obtain a potent strain against Listeria monocytogenes. Methodology and results: A total of 68 LAB isolates were selected to evaluate their antimicrobial activity against L. monocytogenes, a foodborne pathogen and a causative agent of listeriosis. The selected isolate was identified and characterized. The isolate C23 from cabbage showed the highest antimicrobial activity against L. monocytogenes ATCC 7644 with inhibition ability of 73.94%. The isolate was closely related to Lactobacillus brevis by 16S rRNA sequencing and subsequently deposited in GenBank with an accession number of MN880215, named as L. brevis C23. The cell free supernatant (CFS) of L. brevis C23 had high tolerance in low pH and was able to withstand up to 60 °C. The proteinaceous nature of the antimicrobial agent was also confirmed through the enzymatic test. The CFS was stable on different detergents as well as bile salts. Under transmission electron microscopy (TEM), the inhibitory effect of CFS against L. monocytogenes was proven by causing cell lysis. Conclusion, significance and impact of study Bacteriocin-like inhibitory substances (BLIS) of L. brevis C23 showed very promising potential in food industrial application.
Lactobacillales ; Listeria monocytogenes ; Foodborne Diseases ; Sprains and Strains