We have immunophenotyped the tumor-infiltrating mononuclear cells, especially the I_2 (HLA-DR) antigen and OKT_9 antigen expression on lymphocyte infiltrates to determine the activity of infiltrating T cells in 63 cases of advanced stomach and colon cancer. In addition, 18 specimens for biopsies from patients with chronic gastritis and colitis were used as controls. In most cancer specimens, the infiltrating T cells were defficient of I_2 antigen, only 10 out of 63 cases had about 10--30% I_2 positive infiltrating T cells, and nearly no OKT_9 positive infiltrating T lymphocytes. This finding is quite different from that in nomal mucosa and chronic inflammatory mucosa, with more I_2 positive lymphocytes in their infiltrates, indicating that the infiltrating T lymphocytes in tumor tissue inhibited by ome inhibitors present in their environment and contrabuting to the explanation why the tumor mmunity can not control the growth and spread of the tumor and lead to the elimination of the tumor.

Localization of MG_7 antigen was investigated in situ by immunohistochemic and immunoclectron microscopic technique in 62 cases of gastrointestinal carcinoma (GICA). The detective rate of MG_7 antigen was 80. 77% in gastric carcinoma (21/26 cases) and 94.44% in colorectal carcinoma (34/36 cases). Its expressing intensity and distribution were associatcd with the differentiation of tumor cells. Most of the poorly differentiated tumors showed much stronger MG_7 antigen expression and cytoplasmic staining, while most of the differentiated tumors showed membranic or mixed staining, mainly distributed in lumen side. Immunoelectronimicroscopic study revealed that MG_7 antigen predominantly existed in lumen margin microvilli, cell membrane and cytoplasm bencath the cell membrane in well differentiated tumor cells, existed in secretory granules, Golgicomplex and endoplasmic reticulum in poorly differetiatd tumor cells. The finding suggest that MG_7 antigen be a kind of secretory substance syntheszed by GICA cells. The mutual relationship between MG_7 antigen localization and tumor cell differetiation as well as its clinical significance were also discussed.

The mononuclear cells in early skin lesions of 10 patients with extensive psoriasis vulgaris in progressive stage were studied by biotin-avidin-peroxidase method using monoclonal antibodies. The results show that increased mononuclear cells in the early skin lesions, expressing HLA-DR antigen mostly, consist of T lymphocytes and macrophases with a preponderance of T lymphocytes, B lymphocytes are absent, activated T lympocytes are usually in close proximity to DR~+ cells including dentritic cells and keratinocytes, indicating that the initial psoriatic lesion correlates closely with imbalance of cellular immunity.

OBJECTIVE: To analyze the false-negative proportion and reasons of positioning test in benign paroxysmal positional vertigo (BPPV). METHOD: Sixty-eight cases were reviewed and analyzed during February to April 2009. All patients had typical clinical history of BPPV and diagnosed by positioning test,and all were treated with PRM successfully. If positioning test at the first time were negative, examinations repeated after either head shaking or return visits. RESULT: Thirteen patients (19.12%) appeared false-negative in positoning test, and 11 cases showed typical nystagmus after head shaking,while 2 cases were diagnosed by return visitis the next day. There were no statistically difference between false-negative group and positive group in age (P > 0.05), gender (P > 0.05), course of disease (P > 0.05) and types of semi-circular canal (P > 0.05). CONCLUSION Re-examinations after head shaking or return visits are useful for the diagnosis in the suspected BPPV patients who have negative positioning test.
Benign Paroxysmal Positional Vertigo ; diagnosis ; therapy ; False Negative Reactions ; Humans ; Patient Positioning

OBJECTIVE: To compare the vertigo controlling situation between the endolymphatic sac decompression(ELSD) and semicircular canal occlusion (SCO) in stage 4 Ménière disease. METHOD: Fourteen patients who underwent endolymphatic sac decompression and 9 patients who underwent semicircular canal occlusion from 2009 to 2013 were followed. All patients has complete preoperative examination and postoperative follow-up. RESULT: The vetigo control of the patients underwent endolymphatic sac decompression: completely control 35.7%; basic control 28.6%; partly control 14.3%; the vetigo control of the patients underwent semicircular canal occlusion: completely control 88.9%; basic control 11.1%. CONCLUSION ELSD and SCO are alternative methods for the patients of stage 4. SCO has a much highter complete vertigo control rate. For the patients without practical listening, SCO is a better choice.
Endolymphatic Sac ; surgery ; Humans ; Meniere Disease ; surgery ; Otologic Surgical Procedures ; methods ; Semicircular Canals ; surgery ; Vertigo ; surgery