OBJECTIVE:To investigate the characteristics and regularity of ADR in our hospital,and to provide reference for clinical rational drug use. METHODS:262 ADR reports of the hospital during 2013-2014 were summarized and analyzed. RE-SULTS:Among 262 cases of ADR,most of ADR occurred in elderly patients aged above 60 years old,accounting for 46.56%. 239 cases were induced by intravenous drip,accounting for 91.22%;anti-infective drugs induced 97 cases of ADR,accounting for 37.02%,among them there were 48 cases of cephalosporin-induced ADR,accounting for 49.48%(total 97 cases);most of them were lesion of skin and its appendants,being 96 cases and accounting for 36.64%. Among 262 cases,132 cases were improved af-ter drug withdrawal(50.38%);no drug sequelae or death cases was found. CONCLUSIONS:To master the characteritics and reg-ularity of ADR contribnte to redcue and aroid the repeat of ADR.

Objective To detect the level of Tnl and T.2 type cytokines in the patients with lym-phoma in order to find out the laboratory evidence of tumor immunotherapy. Methods The levels of serum IFN-γ, TNF-α, IL-2, IL-4, IL-5 and IL-10 were measured by cytometric bead array (CBA) in 92 patients with lymphoma, and 70 normal sera as control. Results The levels of TH1 type cytokincs in 92 patients with lymphoma were: IFN-γ (34.26 ± 33.48) pg/ml, TNF-α (8.17 ± 10. 09) pg/ml, IL-2 (3.74 ±1.72) pg/ml; and the levels of TH2 type cytokines were: IL-10 (6. 28±8.56) pg/ml, IL-5 (3.53 ±3.20) pg/ml, IL-4 (6.22±7.13) pg/ml. The levels of TH1 and TH2 cytokines in lymphoma patients were significantly higher than those in controls(P ＜ 0.01) except TNF-α. And the rate of IL-2/IL-4 was signifi-cantly decreased in lymphoma patients(P ＜0.01). The level of IL-10 in Ⅲ/Ⅳ stage lymphoma patients was much higher than that in Ⅰ/Ⅱ stage patients(P ＜0.01). The level of IFN-γ/was significantly decreased in aged patients with lymphoma (P ＜0. 05). Conclusion TH 1/TH2 is imbalance in lymphoma, which may provide clinical index for the evaluation of progrossien and prognosis. In the lymphoma patients TH1/TH2 shifts to TH2, which may be the mechanism of tumor arising and transferring by immune escape from immu-nosurveillance.

AIM:To establish some HPLC-digitized fingerprint spectrum(HPLC-DFPS)in Liuwei Dihuang Dripping Pill preparation and to apply it to identification of medicinal materials.METHODS:Based on loganin and paeonoside contents adopted as qualitative and quantitative factors,relative retention value as creteria,RP-HPLC was carried out on inertsil ODS:column,mobile phase consisted of methanol-water(0.2% formyl acid),detection wavelength was set at 240 nm.RESULTS:Using above optimum chromatograph conditions,HPLC-DFPS of relative medicinal materials and "Liwei Dihuang Dripping Pill" preparation were established.By comparison a group of characteristic peaks could be used to identify medicinal materials.CONCLUSION:This result indicates that it is practical to use HPLC-DFPS for identification of Liuwei Dihuang Dripping Pill.

Objective:To establish the drug utilization evaluation(DUE)criteria for isophane protamine biosyntheti(30R)to provide reference for the rational drug use. Methods:Referred to the domestic and foreign literatures and the authorized guidelines for diabetes treatment,and combined with the Delphi expert consultation method,the drug utilization evaluation criteria was established,and after the evaluation,intervention and revaluation,the final criteria were determined. Results:The drug utilization evaluation criteria for isophane protamine biosyntheti(30R)contained three parts,including medication indications,medication process and medication results,which showed the clinical feasibility. Conclusion:The established DUE criteria for isophane protamine biosyntheti(30R)can not only be applied in the drug utilization evaluation,but also provide reference for the DUE in medical institutions.

Objective To study the changes of serum levels of soluble vascular adhesion protein (sVAP-1), hyaluronic acid (HA) in chronic hepatitis C (CHC) patients, to analysis the relationships between serum sVAP-1 and liver function、HA ,to explore the role of sVAP-1 on the pathogenesis of CHC. Method In our research, 88 cases CHC patients were divided into 2 groups,30 cases serum HCV RNA negative (CHC1 gruop)、58 cases serum HCV RNA opsitive (CHC2 gruop);30 cases healthy individuals were enrolled in our research;the serum levels of sVAP-1 and HA were determined by ELISA; Liver function was assayed by automatic biochemistry analyzer;HCV RNA load was measured by real-time polymerase chain reaction (PCR). Results In CHC1,CHC2 and healthy control groups,serum level of sVAP-1 were (112.75 ± 39.00),(154.24 ± 45.88)and (72.23 ± 35.82) ng/mL(F=38.76,P<0.01),respectively; serum level of HA were(87.03 ± 24.95),(132.98 ± 33.54)and (75.07 ± 24.09)ng/mL,respectively (F=47.44,P<0.01);The concentrations of serum sVAP-1、 HA gradually had been increasing with level of ALT (all P<0.05);Especially,significantly positive associations were highly between serum sVAP-1 and ALT 、AST and HA (r=0.711、0.628、0.816, all P<0.001). Conclusion Serum sVAP-1 may be closely related to the hepatic cell inflammatory injury and liver fibrosis in CHC patients.

AIM:To establish some HPLC-digitized fingerprint spectrum(HPLC-DFPS)in Liuwei Dihuang Dripping Pill preparation and to apply it to identification of medicinal materials.METHODS:Based on loganin and paeonoside contents adopted as qualitative and quantitative factors,relative retention value as creteria,RPHPLC was carried out on inensil ODS:column,mobile phase consisted of methanol-water(0.2% formyl acid),detection wavelength was set at 240 nm.RESULTS:Using above optimum chromatograph conditions,HPLC-DFPS of relative medicinal materials andLiwei Dihuang Dripping Pillpreparation were established.By comparison a group of characteristic peaks could be used to identify medicinal materials.CONCLUSION:This result indicates that it is practical to use HPLC-DFPS for identification of Liawei Dihuang Dripping Pill.

Objective To investigate the distribution of age and relationship of ESR with HCT.Methods The HCT,age and ESR datas of the patients which all ages were above 40 years were Collected,then count the mean and standard deviation x±s with different groups of HCT,t test was made to identify the significance of difference.Results HCT:The levels of hct in males was 0.47±0.26 in higher groups of HCT,0.39±0.27 in normal groups and 30.01 ±3.59 in lower groups.The levels of HCT in fe-males was 0.42±0.17 in higher groups,0.30±0.36 in normal groups,0.27±0.45 in lower groups;Age:The average age in males was 57.41±10.62 years in higher HCT groups of patients,67.23 ±12.75 in lower hct groups,there were significantly difference between them.The avarage age in females was 60.70 ± 11.60 in higher HCT groups of patients,61.60 ± 12.40 in lower HCT groups,there were no significantly difference between them.ESR:The levels of ESR in males was (3.95 ±3.26)mm/h in higher HCT groups,(61.61±40.04)mm/h in lower HCT groups;The ESR in females was (28.26±28.62)mm/h in higher HCT groups, (60.20±43.71)mm/h in lower HCT groups,there were significantly difference between different groups included both males and femals.Conclusion When the HCT were decreased,the age and ESR were increased in males,the ESR were also increased but had no relationship with ages in females.Conjuction the age and ESR,it could improve the prevention and monitoring in heart-brain blood disease through the HCT.

Objective To evaluate CT and bronchofiberscopic value in diagnosing bronchogenic stenosis.Methods The CT and bronchofiberscopic appearances of bronchogenic stenosis in 45 cases were analysed comparatively with the results of the pathological examinations.Results Among the 45 cases, there were central pulmonary carcinoma in 25 patients, endobronchial tuberculosis in 10 patients, bronchogenic pneumonia in 9 patients and radiation pneumonia in 1 patient all confirmed pathologically. Conclusion CT and bronchofiberscope are of respectively advantages in diagnosing bronchogenic stenosis,combining both of them,the diagnosis and differential diagnosis of bronchogenic stenosis can be improved.

Objective: To ascertain the process condition of extraction Mashi Granules(Herba Ephedrae, Gypsum Fibrosum, Radix Puerariae, Herba Methal, Semen Armeniacae Amerum, etc.) Methods: In combination with area under the curve of hypothermy in rabbits, process water, macerating time and boiling times were used as parameters in uniformidy design. And then appropriate excipients were selected on the basis of hygroscopicity and granulation. Results: 20 times amount of water as much as herbs was added, macerating time reached as far as 40 min, and extracted 3 times, 2h a time. Excipient formula consisted of lactose and mannitol(4∶1, w/w). Conclusion: The experimental results provide the basis for the ascertainment of extracting process and formulation of Mashi Granules.

Objective:To revise the Chinese version of the Parental Psychological Flexibility Questionnaire (PPFQ) and test its validity and reliability.Methods:Totally 1015 parents from 2 primary school of Beijing were recruited and asked to complete the Chinese version of PPFQ.Item analysis was conducted to identify valid items.The parents were randomly allocated into two groups where one subset (n =510) was used for exploratory factor analysis (EFA),and the other (n =505) for confirmatory factor analysis (CFA) and discriminant validity.In addition,responses of 389 participants selected by simple random sampling was assessed in criterion validity with the Cognitive Fusion Questionnaire (CFQ),Acceptance and Action Questionnaire-Second Edition (AAQ-Ⅱ]),Self-Rating Anxiety Scale (SAS),Self-Rating Depression Scale (SDS),Mindful Attention Awareness Scale (MAAS),Trait Coping Style Questionnaire(TCSQ) and Conners Child Behavior Rating Scale (CBRS).Seven weeks later,225 of the participants were retested.Results:The exploratory factor analysis extracted 3 components,and explained 58.1％ of the total variance.The factor loading of items ranged between 0.63 and 0.86.The confirmatory factor analysis verified the 3-factors model (x2/df=1.77,NFI =0.94,IFI =0.97,GFI =0.96,TLI =0.96,CFI =0.97,RMSEA =0.04).Discriminant validity testing demonstrated the three revised factors had adequate discriminant validity in relation to one another (Ps ＜ 0.01).Criterion validity results showed that the total and subscale scores of the PPFQ were negatively correlated with the scores of CFQ,AAQ-Ⅱ,SAS,SDS,TCSQ-NC,CBRS (r =-0.16--0.52,Ps ＜ 0.01),and were positively correlated with the scores of MAAS,TCSQ-PC (r =0.14-0.37,Ps ＜ 0.01).The total and subscale scores of the PPFQ demonstrated good internal consistency Cronbach's α were 0.77-0.86 and test-retest reliability coefficient were 0.55-0.75.Conclusion:It suggests tthe validity and reliability of the adapted Chinese version of the Parental Psychological Flexibility Questionnaire be satisfactory,and could be used in the clinical and research work in China.