1.Effect of up-regulated esp1 gene expression on apoptosis of A549 cells
Journal of Third Military Medical University 2003;0(08):-
Objective To construct an expression vector containing esp1 in order to observe the effect of esp1 gene on the apoptosis of A549 cells. Methods A conditional plasmid vector containing esp1 was constructed, and transfected into A549 cells. The expression level of esp1 was detected by fluorescence microscopy, the chromosomes and the apoptosis, proliferation were detected. Results Transfection of IRE-EGFP-esp1 into A549 cells reduced improper chromosome segregation, cell proliferation and increased the apoptosis of A549 cells. Conclusion Up-regulation of esp1 not only has inhibitory effects on the abnormal proliferation and chromatid separation of tumor cells, but induces the apoptosis of A549 cells.
2.Studies on the Seed Germination Characteristic of Dodder (Cuscuta L.)
Chinese Traditional and Herbal Drugs 1994;0(12):-
The Seedcoat structure of Cuscuta L. was damaged by treating the seed with various organic solvents or concentrated sulfuric acid for different durations of time or by mechanicalmethods. It was found that the surface layer and hilum were erosion-nonresistant; the palisades had orderly arranged thick cell walls and contain phenols. They are th e main barrier controlling the entrance of water through seedcoat. Erosions with concentrated sulfuric acid are effective methods for overcoming its impermeability and raisiug germination capacity of seeds.
4.Establishment of clinical nursing pathway for artificial abortion and effect assessment
Xiaomei WANG ; Yuehong LIU ; Xiangqian LIU ; Lisha LI ; Chengyun FENG
Chinese Journal of Practical Nursing 2011;27(20):5-7
Objective To build clinical nursing pathway for artificial abortion operation and explore the effect of this method. Methods The nursing pathway was designed and carried out. The satisfaction degree with nursing, status of patients' awareness about contraception and artificial abortion operation was compared before and after the nursing pathway was implemented. Results Before and after the nursing pathway was implemented,the proportion of missing item of operation record was 0.636 and 0.013, the satisfaction degree with nursing was 77.0% and 92.3%, rate of patients' awareness about contraception and artificial abortion operation was 47.6% and 96.1%. Conclusions The clinical nursing pathway for artificial abortion operation may improve the degree of satisfaction- and the status of patients' awareness about contraception and artificial abortion operation, and decline the proportion of missing item of operation note, it ensures high nursing quality and safety surgery, which is worthy of popularizing.
5.Research progress on animal models of depression and their evaluation methods
Tao XUE ; Lisha WU ; Xinmin LIU ; Qiong WANG
Acta Laboratorium Animalis Scientia Sinica 2015;(3):321-326
Depression, a seriously harmful disease to peoples ’ physical and mental health , is prevailing world-wide.But the etiology and pathological mechanism is still not fully uncovered .With the development of social economy and the pace of life speeding up , increasing pressure and vulnerable emotion cause depression incidence increased rapidly .For the further research on depression , study of depression mechanism and antidepressant drugs highly depends on effective ani -mal models .The degree of similarity of animal models to human disease status and the accuracy and reliability of evaluation methods of animal models directly influence the value of the resuts of experiments .In this article we introduce the common-ly used animal models of depression , the evaluation indicators at organism , organ, and molecular levels , and to provide theoretical references for the further studies on depression .
6.Dickkopf-1 inhibits vasculogenic mimicry formation of colon can-cer and relevant mechanism
Lisha QI ; Zhiyong LIU ; Wangzhao SONG ; Runfen CHENG ; Baocun SUN
Chinese Journal of Clinical Oncology 2015;(20):1007-1011
Objective:To investigate the inhibitory effect of Dickkopf-1 (Dkk1) on vasculogenic mimicry (VM) formation and the relevant mechanism. Methods:CD34-PAS dual staining and immunohistochemical staining were used to detect and analyze the re-lationship between VM existence and Dkk1 expression in 217 human colon cancer tissue samples;three dimensional (3D) culture was used to detect the influence of Dkk1 on tube structure formation and on VE-cadherin expression;a subcutaneous mouse xenograft mod-el was made to further validate the inhibitory role of Dkk1 on VM formation in vivo. Results:VM-positive samples indicated a lower expression of Dkk1(P<0.05);colon cancer cells with Dkk1 overexpression exhibited a decreased ability to form tube-like structure and a decreased expression of VE-cadherin;Dkk1 inhibited the VM-formation abilities of human colorectal carcinoma cell line xenograft tu-mor tissue. Conclusion:Dkk1 inhibits the VM formation of colon cancer.
7.Study on determination methods of chymosin potency
Jing LI ; Yue WANG ; Lisha LIU ; Xue REN ; Huihong FAN
Chinese Journal of Biochemical Pharmaceutics 2015;35(10):118-121
Objective To investigate the influencing factors and improve potency methods of chymosin, to verify the stability and applicability of the national standard of chymosin.Methods The effects of different formula milk powder substrate and enzyme concentration on the determination of the activity of chymosin were studied.3 ×3 dose-response parallel line method was established.The results were compared with the different methods of absolute and relative methods.Results The different formula milk powder had a significant effect on the determination of the absolute potency of the activity of chymosin.The concentration of the enzyme was a power function relationship with the milk clotting time.Compared with the absolute potency, reproducibility of the relative potency of the results was better in different laboratories.The suitable doses in 3 ×3 dose-response parallel line method were 0.35,0.44,0.55U/mL.The confidence limit rate was less than 5%.The potency of the national standard of chymosin (140712-201302) was not significantly different between 2013 and 2015.In a certain dose range, the dose-response of the national standard of chimosin and gastropylor complex or lamb'tripe extract was linear, and the two lines were parallel.Conclusion A lot of factors can affect on the potency of chymosin.Relative potency is determinate by reference standard which can eliminate the influence of different substrates, different operators and endpoint judgment on the determination in order to make results have comparability between laboratories.The test design of 3 ×3 dose-response parallel line can control the test deviation better than the single point determination.The stability of the national standard of chymosin(140712-201302) is good, and is suitable for the potency of chymosin of the products of gastropylor complex and the extract of the lamb.
8.Expression and significance of changes of TLR4 and IL-6 in hippocampal CA1 area of rats after ischemia adaptation in distance
Jiang ZHANG ; Lisha CHANG ; Dali WANG ; Tao ZHOU ; Huiying LIU
Chinese Journal of Immunology 2015;(8):1045-1048
Objective:To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning(RIP) and explore its significance.Methods: All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly.Each group was divided into 4 time points:12h,24 h,48 h and 72 h group.There were 6 rats in each group.Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group.The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min, repeat 3 times.To observe the pathological variation of hippocampus CA1 region by HE dyeing;to test the expression of TLR4 and IL-6 by immunohistochemical staining.Results: Compared to contrast group, neuronal loss and swelling reduced significantly in RIP group.Compared to sham group, the TLR4 and IL-6 expression in contrast group and RIP group increased significantly ( P<0.05 ) .Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly(P<0.05).Conclusion:RIP dose have protective effect on cerebral ischemia.The effect may be associated with the inhibition of TLR4 and IL-6 expression.
9.Determination of the activity of cytochrome C by the chromogenic substrate method
Lisha LIU ; Yue WANG ; Jing LI ; Feifei LI ; Huihong FAN
Chinese Journal of Biochemical Pharmaceutics 2015;37(7):138-140
Objective To establish the chromogenic substrate method to determine the activity of cytochrome C.Methods Used TMB as the chromogenic substrate, reacted at 37 ℃ for 15 min, generated the yellow products, and detected the absorbance at 450 nm.The experimental design method is the 4 ×4 parallel line quantitative analysis.ResuIts The activities of cytochrome C injection samples have been determined.The linear regression equation was Y=0.9875 X -1.0221,R2 =0.9996.The accuracy and repeatability were 1.1 % and 3.6 %.ConcIusion The chromogenic substrate method was simple operation, sensitive and can be used to determine the activity of cytochrome C.
10.Molybdenum Target X-ray Diagnosis of Ductal Carcinoma in Situ of Breast:A Report of 20 Cases
Chunhua CHEN ; Lisha LIU ; Cuiyan ZHENG ; Haiyan LIN
Journal of Practical Radiology 2001;0(09):-
Objective To study radiologic (molybdenum target X-ray) findings of ductal carcinoma in situ (DCIS) of breast.Methods The molybdenum target X-ray findings of breast DCIS confirmed by operation or pathology in 20 cases were analyzed.All cases were examined with molybdenum target X-ray at axial and lateral oblique positions in both breasts.Results Among 20 patients,16 cases presented calcifications in the lesions,3 cases showed simple masses,1 case appeared as small dense focus.Conclusion Calcifications are mainly X-ray appearances of DCIS,special attention should be payed to the signs such as masses,small dense focus and so on.