Objective To construct an expression vector containing esp1 in order to observe the effect of esp1 gene on the apoptosis of A549 cells. Methods A conditional plasmid vector containing esp1 was constructed, and transfected into A549 cells. The expression level of esp1 was detected by fluorescence microscopy, the chromosomes and the apoptosis, proliferation were detected. Results Transfection of IRE-EGFP-esp1 into A549 cells reduced improper chromosome segregation, cell proliferation and increased the apoptosis of A549 cells. Conclusion Up-regulation of esp1 not only has inhibitory effects on the abnormal proliferation and chromatid separation of tumor cells, but induces the apoptosis of A549 cells.

The Seedcoat structure of Cuscuta L. was damaged by treating the seed with various organic solvents or concentrated sulfuric acid for different durations of time or by mechanicalmethods. It was found that the surface layer and hilum were erosion-nonresistant; the palisades had orderly arranged thick cell walls and contain phenols. They are th e main barrier controlling the entrance of water through seedcoat. Erosions with concentrated sulfuric acid are effective methods for overcoming its impermeability and raisiug germination capacity of seeds.

Objective To build clinical nursing pathway for artificial abortion operation and explore the effect of this method. Methods The nursing pathway was designed and carried out. The satisfaction degree with nursing, status of patients' awareness about contraception and artificial abortion operation was compared before and after the nursing pathway was implemented. Results Before and after the nursing pathway was implemented,the proportion of missing item of operation record was 0.636 and 0.013, the satisfaction degree with nursing was 77.0% and 92.3%, rate of patients' awareness about contraception and artificial abortion operation was 47.6% and 96.1%. Conclusions The clinical nursing pathway for artificial abortion operation may improve the degree of satisfaction- and the status of patients' awareness about contraception and artificial abortion operation, and decline the proportion of missing item of operation note, it ensures high nursing quality and safety surgery, which is worthy of popularizing.

Objective To investigate the correlation and clinical diagnosis significance between ultrasound elastography and the distribution of myofibroblast in breast tumor.To assess the value of ultrasonic elastography and myofibroblast in the diagnosis of breast cancers.MethodsThree-hundred and fifteen patients recruited from May 2009 to November 2010 were divided into benign group and malignant group according to postoperative pathological diagnosis results considered as gold standard.The clinical value of the score of ultrasonic elastography was evaluated.The expression levels of CD34 and α-SMA protein in breast tissues were examined by immunohistochemistry.ResultsThe difference was statistically significant for the expression level of CD34 and α-SMA between malignant breast tumor patients compared with benign ( P ＜0.05).The expression level of CD34 was lower,but α-SMA was higher in the patients of breast cancer compared with benign tumor.However,the expression level of CD34 and α-SMA was just the opposite in the patients of benign tumor.The expression level of CD34 was the negative correlation with the elastography score ( P ＜0.05) in the breast tumor,but α SMA just the opposite situation.Conclusions The score of ultrasound elastography can represent MFS distribution characteristics in breast tumors in the result that α-SMA +/CD34- can determine the existence of myofibroblast.

Objective To investigate the effect of methylation of the APC gene on expression and the correlation with clinical data in pancreatic cancer.Methods Sixty postoperative tissue samples with pancreatic cancer were collected in the First Affiliated Hospital of Zhengzhou University from August 2010 to January 2011,20 benign pancreatic disease tissues were collected as control groups.APC promoter methylation and gene expression levels were detected by Methylation Specific PCR (MSP),Real Time PCR (RT-PCR) and Western blot in 60 pancreatic carcinoma,42 metastasis and 20 benign pancreatic disease tissues,then analyze the relation between methylation of the APC gene and the clinical data.Results APC promoter methlation was observed 48.53％,46.67％ and 1.16％ in pancreatic carcinoma,metastasis and benign pancreatic disease tissue,respectively.Methylation of APC in pancreatic carcinoma and metastasis increased significantly compared with control tissues (x2 =12.903,14.402; P ＜ 0.05).There were no statistically significant differences of APC expression in these tissues (P ＞ 0.05).There was a significant correlation between methylation of APC and clinicopathological stage (x2 =6.801,P ＜ 0.05),but no correlation with gender,age,tumor size,histological grade and metastasis (x2 =0.727,1.311,0.372,0.148,0.017 ; P ＞ 0.05).Conclusion The methylation of APC gene is closely related with pancreatic carcinoma inogenesis and the clinicopathological stage,but do not effect the expression of APC in tissues.

Objective To study influences of magnetic field on behavior of depressive rats under chronic unpredictable mild stress,and possible mechanisms under such phenomena.Methods Thirty-six Wistar rats were divided into model group,magnetic field group and control group (n =12 in each group) according to random number table.The model group and the magnetic field group received CUMS stimulation daily for 5 weeks,while the control group received no any treatment.At the end of the fifth week,rats in the magnetic field group were placed in the center of a magnetic machine (400 mT),and the model group was raised freely for4 weeks.The severity of depressive behaviors,cortisol levels and contents of C-reactive protein in blood and urine sample were tested pre-treatment,at the end of 5 weeks and 9 weeks.Results After 5 weeks,consumption of glucose solution of the control group was higher than the other two groups (P ＜ 0.05).After 5 and 9 weeks,body weights of the magnetic group and the model group were lower than the control group (P ＜ 0.05).At the end of 9 weeks,performance of the magnetic group as indicated by frequency of grid crossing,grooming time and suspending tail test were improved to a significantly greater extent than the other two groups (P ＜ 0.05).In addition,the urine content of cortisol in 24 hours in magnetic field and model groups was higher than the control group at the end of 5 weeks,and the serum level of cortisol in the magnetic field group were significantly higher than the model group at the end of 9 weeks (P ＜ 0.05).Conclusion The 400mT magnetic field can reduce the severity of behavior changes of rats with CUMS-induced depression,which might be attributable to its influence on the cortisol levels in blood and urine.

A multi-channel central monitoring system based on B/S mode is designed. It is consistent of the central monitoring server software, the Internet Explorer in the client PC, and a physiological signal collector. The server software can accept two client's request of data transfer, and real-time monitor several patients through Internet. The client uses the B/S mode, and just need to install an ActiveX control. The only thing users should to do is opening the Intemet Explorer, from which the waveform and signals are shown and transferred. The system extends the central monitoring service from hospital to the community, and is benefit for patient's long-term observation and cutting fees. Additionally, it can be a new solution of network based health care devices.

Objective:To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning(RIP) and explore its significance.Methods: All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly.Each group was divided into 4 time points:12h,24 h,48 h and 72 h group.There were 6 rats in each group.Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group.The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min, repeat 3 times.To observe the pathological variation of hippocampus CA1 region by HE dyeing;to test the expression of TLR4 and IL-6 by immunohistochemical staining.Results: Compared to contrast group, neuronal loss and swelling reduced significantly in RIP group.Compared to sham group, the TLR4 and IL-6 expression in contrast group and RIP group increased significantly ( P<0.05 ) .Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly(P<0.05).Conclusion:RIP dose have protective effect on cerebral ischemia.The effect may be associated with the inhibition of TLR4 and IL-6 expression.

Objective To establish the chromogenic substrate method to determine the activity of cytochrome C.Methods Used TMB as the chromogenic substrate, reacted at 37 ℃ for 15 min, generated the yellow products, and detected the absorbance at 450 nm.The experimental design method is the 4 ×4 parallel line quantitative analysis.ResuIts The activities of cytochrome C injection samples have been determined.The linear regression equation was Y=0.9875 X -1.0221,R2 =0.9996.The accuracy and repeatability were 1.1 % and 3.6 %.ConcIusion The chromogenic substrate method was simple operation, sensitive and can be used to determine the activity of cytochrome C.