2.The application of the OSCE evaluation system on the medical skills examination and the evaluation on its intrinsic influencing factors
Zhiqing LIANG ; Jihong BAI ; Lisha YANG ; Jinrong ZENG ; Yigang YUE
Chinese Journal of Medical Education Research 2014;13(12):1234-1238
Objective To investigate the application of OSCE evaluation system on the medical skills examination of clinical medical students and the significance of this system on the training of their medical skills.Methods 20 teachers examed 150 students by the OSCE evaluation system with 4 test stations,by comparing the score of the students of different test stations by one-way ANOVA and evaluating the system by questionnaire survey with Likert 5 on the degree of satisfaction and Likert 3 on effects and intrinsic influencing factors of the system.Results The score of the first and forth test stations was lower than that in the other stations(P<0.05).8/5.48% students and 1/5% teachers were not satisfied with the system.The OSCE evaluation system could exam the psychological diathesis,ability of communication,cooperation,and clinical thinking,practical skill of the students and its effects are moderate (the score was more than 2.0).Evaluation on the intrinsic influencing factors:Students considered the questions were more difficulty in the 2nd,3rd,1st,4th test stations order.4/20% teachers considered the questions of the second test station was easy.8/40% teachers considered the duration of the second test station was too long.More than 70% students and teachers considered the other indexes were rational.Conclusion The OSCE evaluation system can play an effective role in directing the teaching and learning.It can also help to culture the comprehensive capacity of the students.We should gradually improve the design of the system by considering the intrinsic influencing factors.
3.Benign mimickers of prostatic adenocarcinoma: diagnostic features and differential diagnosis.
Liang CHENG ; Wenbin HUANG ; Yuan LI ; Lisha WANG
Chinese Journal of Pathology 2014;43(1):52-58
Adenocarcinoma
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metabolism
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pathology
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Atrophy
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Biomarkers
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metabolism
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Diagnosis, Differential
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Humans
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Male
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Prostate
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pathology
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Prostatic Diseases
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metabolism
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pathology
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Prostatic Hyperplasia
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metabolism
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pathology
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Prostatic Neoplasms
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metabolism
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pathology
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Prostatitis
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metabolism
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pathology
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Xanthomatosis
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metabolism
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pathology
4.Preparation of TPGS-modified artesunate liposomes and their in vitro anti-tumor activity
Cheng HU ; Kun LIANG ; Rui AN ; Xinhong WANG ; Lisha YOU
Chinese Traditional Patent Medicine 2017;39(3):492-498
AIM To prepare D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-modified artesunate liposomes and to investigate the in vitro anti-tumor activity.METHODS The liposomes prepared by thin-film dispersion method were characterized by transmission electron microscopy and particle size analyzer,and the encapsulation efficiency was determined by ultrafiltration centrifugation.The liposomes' cytotoxicity to human hepatoma HepG2 cells was evaluated by MTT method.RESULTS The average particle size,PDI,Zeta potential,encapsulation efficiency,drug loading of the liposomes were 126.7 nm,0.182,-10.1 mV,78.8% and 18.38%,respectively.The liposomes displayed a significant inhibition on HepG2 cells with the IC50 value of 0.034 μmol/mL.CONCLUSION Compared with non-TPGS-modified artesunate liposomes,the TPGS-modified artesunate liposomes prepared by this method afford smaller vesicle size,better stability and higher encapsulation efficiency with stronger in vitro anti-tumor activity.
5.Study on extracting process and formulation of Mashi Granules
Jun LI ; Dianjia SUN ; Jiaguo LIU ; Lisha ZHU ; Liang TENG ;
Chinese Traditional Patent Medicine 1992;0(10):-
Objective: To ascertain the process condition of extraction Mashi Granules(Herba Ephedrae, Gypsum Fibrosum, Radix Puerariae, Herba Methal, Semen Armeniacae Amerum, etc.) Methods: In combination with area under the curve of hypothermy in rabbits, process water, macerating time and boiling times were used as parameters in uniformidy design. And then appropriate excipients were selected on the basis of hygroscopicity and granulation. Results: 20 times amount of water as much as herbs was added, macerating time reached as far as 40 min, and extracted 3 times, 2h a time. Excipient formula consisted of lactose and mannitol(4∶1, w/w). Conclusion: The experimental results provide the basis for the ascertainment of extracting process and formulation of Mashi Granules.
6.Curative evaluation of magnetic resonance spectroscopy on hepatic VX2 carcinoma implanted in rabbit after 5-Fu interventional thermochemotherapy
Liang CHEN ; Lipeng CHEN ; Wenfeng LIU ; Hongzhen WU ; Lisha LAI
Cancer Research and Clinic 2014;26(10):663-666,669
Objective To compare magnetic resonance spectroscopy (MRS) characteristics and changes before and after 5-Fu interventional thermochemotherapy (ITC) as well as to analyze the correlation of Cho/Lip and apoptosis index (AI) in hepatic VX2 carcinoma animal model.Methods 20 rabbit models of hepatic VX2 carcinoma were implanted successfully.Ten days later,as tumor diameter≥ 10 mm,routine scan and 1H-MRS were performed before one day and after 14 days on all of rabbits by Philips 1.5T MR scanner.The changes of Cho peak,Lip peak and the ratio of Cho/Lip before and after the treatment were analyzed.Specimens of AI were examined and compared with the ratio of Cho/Lip.Results The Cho peak and Lip peak were increased before ITC.After ITC,the Cho peak and the ratio of Cho/Lip decreased than before.The Lip peak was further increased than that of before.AI was correlated negatively with the ratio of Cho/Lip (r =-0.56,P =0.02).Conclusions The metabolites of different ROI are matched with the levels and scopes of AI.1H-MRS can be used to evaluate tumor cell apoptosis and energy metabolism changes of the rabbit models of hepatic VX2 carcinoma with the treatment.
7.Effect of Ganoderma polysacchride on intracellular free calcium and intracellular pH in murine T cells
Mingchun LI ; Linsheng LEI ; Qingbiao WANG ; Dongsheng LIANG ; Ziming XU ; Shuqin YANG ; Lisha SUN
Chinese Pharmacological Bulletin 2001;17(2):167-170
AIM To extend the approach of the act ion of ganoderma polysacchride on intracellular signal events in T cells. METHODS Laser scanning confocal microscope imaging of the calcium and p H fluorescent indicator dye Fluo-3/AM and SNARF-1/AM were used to determine th e kinetic changes of [Ca2+]i and [pH]i in murine T cells induced b y a ganoderma polysacchride,designated GLB7. RESULTS It was fou nd that GLB7(20 mg*L-1) could increase [Ca2+]i and [pH]i at 1 min were 334.7%±16.4%(n=3)、171.6%±10.4%(n=3) respectively. T he increase in [Ca2+]i induced by GLB7 was due to the influx of extr acellular Ca2+ and intracellular Ca2+ release through both IP3-se nsitive and IP3-insensitive Ca2+ stores, and increase in [pH]i indu ced by GLB7 was relative to Na+/H+ exchange systems and [Ca2+]i. GLB 7 did not influence [Ca2+]i and [pH]i in murine T cells induced by Con A(3 mg*L-1). CONCLUSION Stimulation of the increase in [Ca2+]i and [pH]i may be an important channel for gano derma polysacchrides to achieve their pharmacological actions.
8.Effects of Ganoderma Polysacchrides on PKC and PKA Activities in Murine T Cells
Mingchun LI ; Dongsheng LIANG ; Ziming XU ; Linsheng LEI ; Qingbiao WANG ; Shuqin YANG ; Lisha SUN
China Pharmacy 2001;12(2):78-81
AIM:To investigated the effects of Ganoderma polysaccthride(GLB7)on protein kinase A(PKA) and protein kinase C(PKC) activitives in murine T cells.METHODS:A new ion-pair reversed-phase high liquid chromatography method was used to determinate the activities of PKA and PKC in T cells.RESULTS:GLB7 could markedly increase the activities of PKA and PKC in murine T cells in a dose-dependent manner.The peak time was at 5 min and 20 min and the activities of PKA and PKC returned to basic level at 20 min and 1.5h respectively.GLB7 could induce translocation of PKC and antagonize the inhibition effect of staurosporine(10μ mol· L-1) on PKC in T cells.CONCLUSION:The immunopotentiating and antitumour effects of Ganoderma polysacchride may be associated with its activation on PKA and PKC in murine T cells.
9.CT/MRI features and pathological analysis of primary renal rare benign tumors
Yingying LIANG ; Xinhua WEI ; Lei MO ; Lisha LAI ; Yuan GUO ; Zhaoxiu WANG ; Xinqing JIANG
Journal of Practical Radiology 2017;33(2):254-257
Objective To improve the diagnostic accuracy of primary renal rare benign tumors by exploring and analyzing the CT/MRI and clinical pathologic features.Methods 9 patients with primary renal rare benign tumors pathologically proven lesions after operation who had CT or MRI exams with contrast were enrolled in our hospital.The radiological and clinical pathological features of all tumors were analyzed respectively.Results The cases were the mixed epithelial and stromal tumor of kidney (MESTK),cystic nephroma(CN),renal leiomyoma(RL)and renal oncocytoma(RO)respectively.Location:6 cases were in left kidney and 3 in right kidney.Size:long diameter 2.5-8.9 cm,mean 5.7 cm;short diameter 2.5-8.4 cm,mean 4.9 cm.The tumor shape included oval(n=7) and irregular(n=2).9 cases protruded from the renal surface.MESTK showed polycystic lesions on preconstrast,various degrees of cystic wall and septum enhancement were detected on enhancement scans.CN showed cystic lesions and calcification on preconstrast, slight septum enhancement were detected on enhancement scans.RL showed heterogeneous density or signal with patchy necrosis and clear border on precontrast,the obvious enhancement was found on cortex and medullary phase and slight washout enhancement was detected on excretory phase.RO showed heterogeneous hypointense on T1 WI,hyperintense on T2 WI and mixed isodensity and hypodensity on CT precontrast.Slight delayed enhancement was found in central scar and washout enhancement was detected in parenchyma on three phases.Conclusion There are some special CT/MRI characteristics for primary renal rare benign tumors,which could improve their diagnosis and differential diagnosis combined with the clinical pathological features.
10.A study of effectiveness and safety of insulin glargine in the treatment of patients with type 2 diabetes mellitus
Qiu CHEN ; Wenqun HAN ; Yongbi LIANG ; Qin ZHANG ; Yao LI ; Zhihuang ZUO ; Lisha SUN
Chinese Journal of Postgraduates of Medicine 2012;35(16):4-7
ObjectiveTo investigate the effectiveness and safety of insulin glargine in the treatment of patients with type 2 diabetes mellitus (T2DM),and verify the new remedy called one central and three steps for T2DM.MethodsUsed multicenter,random,open and self-control study.Two hundred and three cases with T2DM treated with insulin glargine were divided into four groups according to different therapy:30 cases with one needle method,106 cases with one needle and one pill method,48 cases with one long-acting and several short-acting method,and 19 cases with one long-acting,one pill and several short-acting method.The changes of blood glucose,glycosylated hemoglobin (HbA1c),weight and so on before and after treatment were observed.ResultsThe levels of fasting plasma glucose(FPG),2 h postprandial plasma glucose (2hPPG) and HbA1c decreased significantly after treatment than those before treatment[(5.78 ±0.76)mmol/L,(8.37 ±:1.37) mmol/L,(6.81 ±0.38)% vs. (11.73 ±4.49) mmol/L,(16.73 ±4.96) mmol/L,(9.43 ± 2.31 )%,P < 0.01 ].The weight and body mass index had no obvious changes before and after treatment( P > 0.05 ).There was significant difference in the level of FPG,2hPPG and HbA1c before and after treatment in four groups respectively(P<0.01 ).There was only 1 case who occurred hypoglycemia during the treatment.ConclusionThe therapy,one central and three steps,is not only effective and safe,but also convenient and cheap for T2DM.